Usp33 (NM_001076676) Mouse Untagged Clone
CAT#: MC222481
Usp33 (untagged) - Mouse ubiquitin specific peptidase 33 (Usp33), transcript variant 2, (10ug)
CNY 11,590.00
Product images
![](https://cdn.origene.com/img/defaults-img.jpg)
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | 9830169D19Rik; AA409780; Vdu1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC222481 representing NM_001076676
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGACTTTTCGAAATCATTGTCCACATTTGGATTCAGTTGGTGAAATAACGAAAGAGGACTTGATAC AGAAATCTCTCGGCGCTTGTCAGGACTGTAAAGTCAGAGGACCAAACCTGTGGGCGTGCCTGGAGAATAG GTGCTCCTATGTTGGCTGCGGGGAGTCGCAAGTGGACCACAGCACCATACACTCGCAGGAGACAAAGCAT TATCTAACTGTGAACCTCACCACTCTTCGAGTATGGTGTTATGCTTGCAGCAAAGAAGTATTTTTGGATA GAAAATTAGGAACTCCTCCTTCATTACCTCATGTAAGACAGCCTCAACAAACACAAGAAAACAGTGTCCA GGATTTTAAAATTCCCAGTAATCCAGCATTGAAAACCCCCATGGTTGCTGTGTCTGAAGATCTGGATATA GAAGTGGAAGAGGAAGACGAGCTGAAGGCTAGAGGCCTGACAGGTTTGAAAAACATTGGAAATACTTGTT ATATGAACGCGGCGCTGCAGGCTCTTTCTAACTGCCCACCTTTGACACAGTTCTTTCTTGATTGTGGAGG ACTGGCTAGAACAGATAAGAAACCAGCAATTTGTAAAAGTTATCTCAAACTAATGACCGAGTTGTGGCAC AAGAGCAGGCCAGGATCTGTTGTGCCGGCTAATCTGTTCCAAGGAATTAAAACTGTAAATCCAACTTTTC GAGGTTATTCTCAGCAGGATGCCCAGGAATTCCTTCGCTGTCTAATGGACCTGCTTCATGAGGAGCTGAA GGAGCAGGTCATGGAAATGGAGGAAGAGCCTCAAACACTAACTTCTGAGGAGACGGTGGAGGAAGAGAAG AGCCAGTCAGATGTGGATTTTCAGTCGTGCGAGTCTTGTAGCAGCAGCGAGAAAGCAGAAAATGAGAGTG GCTCCAAAGGCTTTCCTGAGGACAGCAATGAGACCACCATGCTCATCCAGGACGAGGATGACCTGGAGAT GGCCAAAGACTGGCAGAAAGAGAAGGTGTGCAATAAGATCAACAAGGCAAATGCCGATGTAGAACTGGAC AAAGACAGGGACACAGTGTGTGAAACAGTTGACCTAAACAGCCAGGAGACCGTCAAAGTGCAGATACACG GCAGAGCCTCAGAATCTATCACTGATGTCCATCTGAATGACCTAGCTACGTCACAGATCCTTCCTTCAAA TGAAAGTGTTAGTCCACGGTTATCAGCAAGCCCTCCTAAGCTAGGCAGTCTGTGGCCAGGACTGTCGCCT CCACACAAGAAAGCTCAGTCTACATCTGCAAAGAGGAAAAAGCAGCATAAGAAATACAGAAGTGTCATCT CCGACATATTCGATGGAACAGTCATTAGCTCGGTACAGTGTCTGACGTGCGATAGGGTGTCTATAACCCT CGAGACCTTTCAGGATCTGTCCTTGCCGATTCCTGGCAAGGAGGACCTGGCTAAGCTGCACTCCTCCAGT CACCCAACTATAGTCAAAGCAGGGTCATGTGGTGAAGCGTACGCCCCGCAGGGGTGGATAGCTTTCTTCA TGGAGTATGTGAAGAGCTGGTTTTGGGGTCCAGTAGTTACCTTGCAAGATTGTCTTGCTGCCTTCTTCGC CAGAGATGAACTTAAAGGTGACAACATGTACAGTTGTGAAAAATGCAAAAAGTTGAGGAATGGAGTAAAG TTTTGTAAAGTACAGAAGTTTCCTGAGATTTTGTGTATCCACCTTAAAAGATTTCGACATGAACTGATGT TTTCCACCAAAATTAGCACCCATGTTTCCTTCCCCCTGGAAGGCCTCGATCTTCAGCCATTTCTTGCAAA GGACAGCCCAGCTCAGATTGTGACATATGATCTCCTGTCAGTCATCTGTCACCATGGGACTGCAAGTAGT GGGCACTACATTGCCTACTGCCGAAACAATTTAAATAACCTGTGGTATGAGTTTGATGACCAGAGCGTCA CTGAAGTTTCAGAGTCCACTGTACAGAATGCCGAGGCCTACGTCCTTTTCTACAGGAAGAGCAGTGAAGA GGCACAAAAGGAGAGGCGGAGGATATCAAATTTGTTGAACATCATGGAACCTAGCCTCCTTCAGTTCTAC ATATCTCGACAGTGGTTGAATAAATTTAAGACCTTTGCTGAACCTGGCCCTATTTCAAATAATGATTTTC TCTGTATCCATGGAGGTATTCCTCCACGAAAAGCGAGTTATATTGAAGACTTAGTTTTGATGCTGCCTCA GAACATTTGGGATAACCTCTATAGCAGGTATGGAGGAGGGCCTGCTGTCAACCATCTCTACATCTGCCAC ACCTGCCAAATTGAGTTAGAGAAGATTGAAAAACGAAGAAAAACCGAATTGGAAATTTTTATTCGGCTCA ACAGAGCATTTCAAGAGGAGGACTCCCCAGCTACTTTTTACTGTATCAGCATGCAGTGGTTTAGAGAATG GGAGAGTTTTGTAAAGGATCCCCCAGGTCCAATCGACAACACTAAAATTGCGGTTACTAAATGTGGCAGT GTGATGCTCAAGCAAGGAGCAGACTCTGGTCAAATTTCAGAAGAAACATGGAATTTCCTGCAGTCTATAT ATGGTGGGGGGCCTGAAGTTATCCTCCGACCTCCAGTTGTTCATGTTGACCCTGATGTACTCCAAGCAGA GGAAAAGATTGAAGTAGAAACTCGCTCTTTGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_001076676 |
Insert Size | 2694 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001076676.2, NP_001070144.1 |
RefSeq Size | 4173 bp |
RefSeq ORF | 2694 bp |
Locus ID | 170822 |
Gene Summary | Deubiquitinating enzyme involved in various processes such as centrosome duplication, cellular migration and beta-2 adrenergic receptor/ADRB2 recycling. Involved in regulation of centrosome duplication by mediating deubiquitination of CCP110 in S and G2/M phase, leading to stabilize CCP110 during the period which centrioles duplicate and elongate. Involved in cell migration via its interaction with intracellular domain of ROBO1, leading to regulate the Slit signaling. Plays a role in commissural axon guidance cross the ventral midline of the neural tube in a Slit-dependent manner, possibly by mediating the deubiquitination of ROBO1. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination of beta-arrestins (ARRB1 and ARRB2) and beta-2 adrenergic receptor (ADRB2). Plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2. Upon dissociation, it is probably transferred to the translocated beta-arrestins, leading to beta-arrestins deubiquitination and disengagement from ADRB2. This suggests the existence of a dynamic exchange between the ADRB2 and beta-arrestins. Deubiquitinates DIO2, thereby regulating thyroid hormone regulation. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses two alternate in-frame splice junctions compared to variant 1, resulting in a shorter protein (isoform 2). |
Documents
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG226447 | Usp33 (tGFP-tagged) - Mouse ubiquitin specific peptidase 33 (Usp33) transcript variant 2, (10ug) |
CNY 9,790.00 |
|
MR226447 | Usp33 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 33 (Usp33), transcript variant 2 |
CNY 8,930.00 |
|
MR226447L3 | Lenti ORF clone of Usp33 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 33 (Usp33), transcript variant 2 |
CNY 10,830.00 |
|
MR226447L4 | Lenti ORF clone of Usp33 (mGFP-tagged) - Mouse ubiquitin specific peptidase 33 (Usp33), transcript variant 2 |
CNY 10,830.00 |