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Eif4g2 (NM_013507) Mouse Untagged Clone
CAT＃: MC222508
Eif4g2 (untagged) - Mouse eukaryotic translation initiation factor 4, gamma 2 (Eif4g2), transcript variant 1, (10ug)
CNY 11,690.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | DAP; DAP-5; E130105L11Rik; Na; Nat; Nat1; Natm1; p97 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC222508 representing NM_013507
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC GTGGAGAGTGCGATTGCAGAAGGGGGTGCTTCTCGTTTCAGTGCTTCTTCGGGCGGAGGAGGAAGTAGGG GTGCACCTCAGCACTATCCCAAGACTGCTGGCAACAGCGAGTTCCTGGGGAAAACCCCAGGGCAAAACGC TCAGAAATGGATTCCTGCACGAAGCACTAGACGAGATGACAACTCCGCAGCAAACAACTCCGCAAATGAA AAAGAACGACATGATGCAATCTTCAGGAAAGTAAGAGGCATACTAAATAAGCTTACTCCTGAAAAGTTTG ACAAGCTATGCCTTGAGCTCCTCAATGTGGGTGTAGAGTCTAAACTCATCCTTAAAGGGGTCATACTGCT GATTGTGGACAAAGCCTTAGAAGAGCCAAAGTATAGCTCGCTGTATGCTCAGCTATGTCTGCGATTGGCA GAAGATGCACCAAACTTTGATGGCCCAGCAGCAGAGGGTCAACCAGGACAGAAGCAAAGCACAACATTCA GACGCCTCTTGATTTCCAAATTGCAAGATGAATTTGAAAACCGAACCAGAAATGTTGATGTCTATGATAA GCGTGAAAATCCCCTCCTTCCTGAGGAGGAGGAACAGAGAGCCATTGCTAAGATCAAGATGTTGGGGAAC ATCAAATTCATTGGAGAACTTGGCAAGCTTGATCTTATTCATGAATCTATCCTTCATAAGTGCATCAAAA CACTTTTGGAAAAGAAGAAGAGAGTCCAACTCAAAGATATGGGAGAGGATTTGGAGTGCCTCTGTCAGAT AATGAGGACAGTGGGACCTCGATTAGACCATGAACGAGCCAAGTCCTTAATGGATCAGTACTTTGCCAGA ATGTGTTCCTTAATGTTAAGTAAGGAATTGCCAGCCAGGATTCGTTTCCTACTGCAGGATACTGTAGAGT TGCGAGAGCACCATTGGGTTCCTCGCAAGGCTTTTCTTGACAATGGACCAAAGACGATCAATCAAATCCG TCAAGATGCAGTAAAAGATCTAGGAGTGTTTATTCCTGCTCCTATGGCTCAAGGGAGGAATGACTTCTTC CTGGAGGGACCGTTCATGCCGCCAAGGATGAAAATGGATAGGGACCCACTTGGGGGACTTGCTGATATGT TTGGACAAATGCCAGGTAGTGGAATTGGTACTGGTCCAGGAGTTATCCAGGATAGATTTTCACCCACAAT GGGACGTCATCGTTCAAATCAGCTCTTCAATGGCCATGGGGGGCACATCATGCCTCCCACGCAATCGCAG TTTGGAGAGATGGGGGGCAAGTTTATGAAAAGCCAGGGGCTAAGCCAGCTCTACCATAACCAGAGTCAGG GACTCTTATCCCAGCTACAAGGACAGTCGAAGGATATGCCACCTCGGTTTTCTAAGAAAGGACAGCTTAA TGCAGATGAGATTAGTTTGAGGCCTGCTCAGTCGTTTCTAATGAATAAAAATCAGGTGCCAAAGCTTCAG CCCCAGATAACTATGATTCCTCCCAGTGCACAGCCACCACGCACTCAAACACCGCCTCTGGGACAGACAC CTCAACTTGGTCTCAAAACTAATCCACCACTTATCCAGGAAAAGCCTGCCAAGACTAGCAAAAAGCCACC ACCATCAAAGGAAGAACTACTTAAACTGACCGAAGCCGTTGTGACTGACTATCTGAACAGTGGAAATGCC AACGACGCTGTCAGTGGTGTGAGAGAAATGAGAGCTCCAAAACACTTTCTTCCTGAGATGCTAAGCAAAG TGATCATCCTGTCACTTGATAGAAGTGATGAAGATAAAGAAAAAGCAAGCTCTTTAATCAGTTTACTCAA ACAGGAAGGGATAGCCACAAGTGACAACTTCATGCAGGCTTTCCTGAATGTATTGGAGCAGTGCCCCAAA CTGGAGGTTGACATCCCCTTGGTGAAATCTTACTTGGCACAGTTTGCAGCTCGTGCTATAATTTCAGAGT TGGTGAGCATTTCCGAACTAGCTCAACCACTGGAGAGTGGCACCCACTTCCCTCTCTTCTTACTTTGTCT TCAACAATTAGCTAAATTGCAAGACCGAGAGTGGTTAACCGAACTTTTTCAACAAAGCAAGGTCAATATG CAGAAAATGCTGCCAGAAATTGATCAGAATAAGGATCGAATGTTGGAGATTTTGGAAGGAAAGGGACTGA GTTTCTTATTCCCACTCCTTAAATTGGAGAAGGAACTATTGAAGCAAATTAAGCTGGATCCATCCCCTCA AACTATATATAAATGGATTAAAGATAACATCTCTCCCAAACTTCATGTAGATAAAGGATTCGTGAACATC TTAATGACCAGCTTCTTACAGTACATTTCTAGTGAAGTAAGCCCACCCAGCGATGAAACAGATTCTTCCT CTGCTCCTTCCAAAGAGCAGTTAGAGCAGGAAAAACAGCTGCTGCTCTCTTTTAAGCCAGTGATGCAGAA ATTTCTTCATGATCATGTGGATCTACAGGTCAGTGCCCTGTATGCTTTGCAGGTGCACTGTTACAACAGC AGCTTCCCAAAAGGCATGTTACTTCGATTTTTTGTTCACTTCTATGACATGGAAATTATTGAAGAGGAAG CTTTCTTAGCTTGGAAGGAAGACATAACTCAAGAGTTTCCAGGAAAAGGCAAGGCTTTGTTCCAGGTGAA TCAGTGGCTAACCTGGCTAGAAACTGCTGAAGAAGAAGAATCAGAGGAAGAAGCTGACTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_013507 |
| Insert Size | 2721 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_013507.3, NP_038535.2 |
| RefSeq Size | 7760 bp |
| RefSeq ORF | 2721 bp |
| Locus ID | 13690 |
| UniProt ID | Q62448 |
| Gene Summary | Translation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F (eIF4F), which is a cap binding protein complex that consists of three subunits: eIF4A, eIF4E and eIF4G. The protein encoded by this gene shares similarity with the C-terminal region of eIF4G, that contains the binding sites for eIF4A and eIF3; eIF4G in addition, contains a binding site for eIF4E at the N-terminus. Unlike eIF4G which supports cap-dependent and independent translation, this gene product functions as a general repressor of translation by forming translationally inactive complexes. Transgene expression of the apolipoprotein B mRNA-editing enzyme (APOBEC-1) causes extensive editing of this mRNA, which could contribute to the potent oncogenesis induced by overexpression of APOBEC-1. In vitro and in vivo studies in human indicate that translation of this mRNA initiates exclusively at a non-AUG (GUG) codon. This also appears to be true for mouse. Two alternatively spliced transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) encodes the longer protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no quality transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG211132 | Eif4g2 (tGFP-tagged) - Mouse eukaryotic translation initiation factor 4, gamma 2 (Eif4g2), transcript variant 1 |
CNY 9,980.00 |
|
| MR211132 | Eif4g2 (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 4, gamma 2 (Eif4g2), transcript variant 1 |
CNY 9,030.00 |
|
| MR211132L3 | Lenti ORF clone of Eif4g2 (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 4, gamma 2 (Eif4g2), transcript variant 1 |
CNY 10,930.00 |
|
| MR211132L4 | Lenti ORF clone of Eif4g2 (mGFP-tagged) - Mouse eukaryotic translation initiation factor 4, gamma 2 (Eif4g2), transcript variant 1 |
CNY 10,930.00 |
