Ern2 (NM_012016) Mouse Untagged Clone

CAT#: MC222523

Ern2 (untagged) - Mouse endoplasmic reticulum (ER) to nucleus signalling 2 (Ern2), (10ug)



  "NM_012016" in other vectors (4)

CNY 11,690.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms Ern1; Ire1; ire1-beta; Ire1b; mIre1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC222523 representing NM_012016
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGAGACCAGTCCAGAGATTTCAACTGTGGTCTCCTCTCGGGTTCCTGCTGCAGCTTGTGACGTTGC
TGGGGAAACTCGGTCCACAGGTCCAGTCTGTCAGGCCAGAGAGCCTTCTGTTTGTGTCTACCTTGGACGG
AAGTCTCCATGCACTGAACAAGCAGACAGGGGACTTGAAGTGGACAGTGAAGGATGACCCCATCATCCAG
GGCCCAATGTATGTCACAGAAATGGCCTTTCTCTCTGACCCAGCTGACGGCAGCCTGTATGTCTTGGGAA
CCCAGAAACAACAGGGGCTGATGAAACTGCCATTCACCATCCCAGAGCTGGTTCATGCCTCTCCCTGTCG
CAGCTCTGATGGTGTCTTCTACACAGGCCGGAAGCAGGATGCCTGGTTTGTAGTGGACCCTGAGTCAGGG
GAGACTCAGATGACACTGACCACAGAAGGCCTCTCTACTCCTCAACTTTTCATTGGCCGAACACAGTACA
CAGTCTCCATGCATGACCTACGGACCCCAGCCCTGCGGTGGAACACAACCTATCGCCGCTACTCAGCACC
CCTCCTGAATGGCTCACCCGGGAAATACATGAGCCACCTGACATCCTGTGGGATGGGCCTGCTTCTCACT
GTGGATCCAGGAAGTGGAATTGTGCTGTGGACACAGGACCTGGGAGTGCCTGTGACAGGGATTTACACAT
GGCACCAGGATGGTCTGCACCAGCTGCCCCATCTCACACTGGCTCGGGACACCCTACATTTCCTTGTCCT
CCGCTGGGGCCACATCCGTCTTCCTGCCTCCAGCTACCAAGACACAGCCACCCAGTTCTCTTCCTTAGAC
ACCCAGCTTTTGATGACACTGTATGTGGGGAAAGAAGAAGCTGGGTTCTATGTCTCTAAAGCACTGGTCC
ACGCTGGGGTGGCCCTCGTGCCCCGTGGACTGACCCTGGCACCCATGGACGGTCCCACAACAGATGAGGT
GACACTTCAAGTCTCGGGGGAGCGAGAGGGCTCACCCAGCACTGCTGTCAGATACCCCTCAGGCAGCGTG
GCCCTCCCCAGCCAGTGGCTACTCATTGGATACCACGAACCTCCCCCCGTTCTACACACAACCATGCTGC
GGGTTCATCCCATCCCAGGGAAAGTGTCTGCTGAGACACGAGCCTCAGAGGACCTCCATGCTCCACCTGT
CTTTTTTGAGCTCCTAAACCTGAGGAGGGAGGATCCAGAACTACATCCTGAAGAGAAAGCTTCAGACTCT
TATCCAGGGCTGGGATCCCAAGACCTGCTAGCAGCTACCTTCACTGCCATCCTCCTGGGAGCCTGGGTCC
TCTACCTGATGAGGCAGCAGCAGCAGAGCCCTTCAGCACCTGCTGGCCCTCCTGACCTCTCACAGGATGC
TCAGGGACAGCTCTCAAGGGACATCTTACAGGACCAGAGGAGATTTCAAAGCCCCTCAGAGCCAGCCCAG
CCACCTCATGACCCTGAAGCAGGGCAGCCCACCGTGGTGGGGAAGATTTCCTTCAACCCCAAGGATGTGC
TGGGCCGTGGGGCAGGAGGGACTTTTGTTTTCCGAGGACAGTTTGAGGGGCGGGCAGTAGCTGTGAAGAG
GCTCCTCCGAGAGTGCTTTGGCTTGGTTCGGCGAGAGGTGCAGCTGCTGCAAGAGTCTGACCGGCACCCC
AACGTGCTTCGCTACTTCTGCACTGAGCATGGTCCCCAGTTCCACTACATTGCCTTGGAGCTCTGCCAGG
CCTCCTTGCAAGAGTACGTGGAGAGCCCAGACCTAGATCGCTGGGGCCTGGAGCCCACCACAGTGCTGCA
GCAGATGATGTCCGGCCTGGCCCATCTACATTCCTTACACATAGTACACCGTGACCTAAAGCCAGCCAAC
ATTCTCATGGCTGGGCCTGACAGCCAGGGCCAAGGCAGAGTGGTCATCTCTGACTTCGGCCTCTGCAAGA
AGCTACCTGTTGGCCGTTGTAGCTTCAGTCTCCATTCTGGTATTCCCGGCACAGAAGGCTGGATGGCACC
AGAGCTCCTGCAGCTCCCCCCAGACAGCCCGACCAGCGCTGTGGATATCTTCTCCGCAGGCTGCGTATTT
TATTACGTGCTTTCCGGTGGCAGCCACCCCTTTGGAGAGAGTCTCTACCGACAGGCCAACATCCTCTCAG
GGGATCCCTGTCTGGCTCAGCTGCAGGAAGAGACCCATGACAAGGTTGTGGCATTGGACTTAGTGAGAGC
CATGCTGAGCTTGCTGCCCCAGGATCGCCCCTCGGCAGGATGGGTACTGGCTCACCCCCTCTTTTGGAGC
AGAGCTAAGGAGCTCCAGTTCTTCCAGGATGTCAGTGACTGGTTGGAGAAGGAACCAGATCAGGGGCCTC
TCGTGTCGGCGCTGGAGGCAGGAAGCTACAAGGTGGTCCGGGAAGACTGGCACAAGCACATCTCAGCCCC
TCTGCAGGCAGATCTGAAAAGGTTCCGCTCATACAAAGGGACATCAGTTCGAGACCTGCTCCGGGCCATG
AGGAACAAGAAGCACCACTACAGGGAGCTCCCAGCTGAGGTGCGCCAGACACTAGGCCAACTTCCTGCTG
GCTTCATTCAGTACTTCACACAGCGATTCCCCAGGCTGTTGCTGCACACTCACCGTGCCATGAGGACCTG
CGCCTCTGAGAGCCTCTTCCTGCCCTACTACCCACCAGCTCTGGAAGCCAGGAGGCCAGATGCCACAAAG
AGCTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_012016
Insert Size 2736 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_012016.3, NP_036146.2
RefSeq Size 2988 bp
RefSeq ORF 2736 bp
Locus ID 26918
UniProt ID Q9Z2E3
Gene Summary Role in expression of the DDIT3 transcription factor, required for the unfolded-protein response, growth arrest and apoptosis. Has no effect on 28S ribosomal RNA cleavage, unlike the corresponding human protein.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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