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Kdm7a (NM_001033430) Mouse Untagged Clone
CAT＃: MC222764
Jhdm1d (untagged) - Mouse jumonji C domain-containing histone demethylase 1 homolog D (S. cerevisiae) (Jhdm1d), (10ug)
CNY 12,070.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | A630082K20Rik; BB041802; ENSMUSG00000073143; Jhdm1d; mKIAA1718 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC222764 representing NM_001033430
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGGAGCGGCGGCGGCGGTGGCCGCGGGAGCAGCAGCTGGAGCCGCCGCGGCAGCGGGGTCGGTGT CCGCTCCGGGTCGGGCCTCGGCGCCCCCTCCGCCTCCGCCCGTGTACTGTGTATGCCGGCAGCCGTACGA CGTGAACCGCTTCATGATCGAGTGCGATGTCTGCAAGGACTGGTTCCACGGCAGCTGTGTTGGCGTGGAA GAGCATCATGCTGTGGACATTGACCTGTACCACTGCCCGGACTGTGCAGCTCTACACGGCTCTTCCTTAA TGAAGAAGAGGAGGAACTGGCACAGGCATGACTACACAGAAGTGGATGATGGCTCCAAACCTGTTCAAGC TGGAACTAGGGCCTTTGTTAAAGAACTACGTTCTCGAGTCTTCCCAAGTGCCGATGAAATCATTGTAAAG ATGCATGGCAGTCAGCTGACACAAAGATATCTGGAGAAACATGGATTTGATGTCCCTATTATGGTCCCTA AATTAGATGACCTTGGACTCAGGCTCCCTTCACCTGCTTTTTCAGTGATGGATGTGGAACGTTATGTAGG TGGTGACAAAGTGATAGATGTTATTGATGTGGCAAGGCAGGCGGACAGCAAAATGACACTTCACAATTAT GTTAAATACTTCATGAATCCTGACAGACCAAAAGTGCTAAATGTGATCAGCCTTGAATTTTCGGATACAA AAATGTCTGAATTGGTGGAGGTTCCTGATATAGCCAGAAAACTTTCATGGGTGGAAAATTACTGGCCAGA TGATTCAGTCTTCCCCAAGCCCTTTGTCCAGAAATACTGCTTAATGGGAGTTCAAGATAGCTATACAGAT TTCCACATTGACTTTGGTGGGACTTCAGTCTGGTACCATGTCCTTTGGGGTGAGAAGATTTTTTATTTGA TAAAGCCAACAAATGAAAATTTGGCACTCTATGAATCTTGGAGTTCATCTGTGACCCAGAGTGAGGTGTT CTTTGGAGATAAAGTAGATAAATGCTACAAATGTGTGGTAAAGCAGGGACATACTTTGTTTGTTCCTACA GGGTGGATCCATGCTGTGCTCACTTCTCAGGACTGTATGGCTTTTGGAGGGAACTTTCTGCACAACCTCA ACATTGGCATGCAGCTCAGGTGTTATGAGATGGAAAAAAGACTAAAAACACCAGATCTTTTCAAATTTCC TTTCTTTGAAGCCATATGCTGGTTTGTAGCCAAAAGTTTGCTGGAAACCTTGAAAGAACTGAAAGAAGAT GGTTTCCAGCCTCAAAGTTACTTAGTACAGGGAGTAAAAGCACTGCATACTGCTTTAAAATTATGGATGA AAAAAGAACTTGTATCTGAACATGCCTTTGAGATTCCAGACAATGTTAGACCTGGGCATCTTATTAAGGA ACTTTCTAAAGTAATTCGAGCAATAGAGGAGGAAAATGGCAAACCAGTTAAATCTCAGGGAATTCCTTCT GTGTGTCCAGTTTCACGGCCTTCAAATGAGGCATCTCCACCATATCATTCCCGGAGAAAGATGAGGAAAC TTCGAGATCATAATGTCCGAACTCCTTCTAACCTTGACATTCTTGAGCTCCATACAAGGGAAGTCCTCAA AAGATTAGAGATGTGTCCATGGGAGGAGGACCTACTGAGCTCTAAACTGAATGGAAAATTCAACAAACAT CTCCAGCCATCTTCCACAGTACCTGAGTGGAGAGCGAAAGATAATGATCTACGATTACTGCTGACAAATG GAAGAATAATTAAAGATGAGAGACAGCTGTTTGCAGATCGAAGTCTTTATACAGCAGATAGTGAAAATGA AGAGGACAAGAAGCCAACACAGAATGCAAACATGAAGACAGAACAGAGCTCAGGAAGAGAGGAGGCCGAA AGTCAAGGCTCTCCCAAACCACTCAACAGAATCTTTACAAGTGTGAGATCGGAACTCAGGAGTAGACCGT CAGAATATTCTGATGGTTCTGATTCAGAAGACTCTGGACCCGACTGCACTGCACTGAAAATTAACTTTGC CACTGAAGATTCTGAAAGTTCAGGTGATGAAAAGAAACATGAAATAACATCACACTTTAAAGAAGAGTCT GATATAGTGAGGAACCTCCTTCAGAAGAGCCAGAAGCCATCTAGACAGGAAATCCCAGTTAAAAGGGAAT GTCCTACCTCGACGAGCACAGAGGAAGAAGCTATTCAGGGCATGCTGTCCATGGCAGGGTTGCACTATTC CTCGTGTTTACAAAGGCAGATACAAAGCACAGATTGCAGTGGTGAGAAGAACTCTCTCCAGGATCCCAGC AGCTGCCACGGCAGTAACCCTGAGTTTAGGCAGTTGTATCGCTGCAATAAACCAGTGGAATTTGGATACC ATGCCAAGACTGAAGATCAGGATTTGATGACTTCTTCCTGGAATAAACAGTTTGATCGAACTTCCAGATT TAATGCTCAGGACTTAAGTAGAAGCCAGAAACACATCAAAAAGGAAAGCTCTTCAGAAATCAATCAGAAG GCACAAAGCAGGCATTGTGTGGACAGCAATAGCTCAAGCATTCAGAATGGAAAGTACACGCTGAATCCCA GCTTGGTTTCATGCCAGATAAGTAATGGCAGCCTGAGCCCAGAAAGGCCTATTGGTGAAACTTCTTTTTC AATGCCCCTTCACCCCACCAAGAGACCTGCATCAAACCCACCACCTATCAGCAACCAGGCAACAAAAGGT AAACGTCCAAAAAAAGGAATGGCAACAGCCAAACAACGTCTTGGGAAGATTCTGAAATTGAACAGAAATG GCCATGCTCGCTTCTTCGTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001033430 |
| Insert Size | 2823 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001033430.4, NP_001028602.2 |
| RefSeq Size | 9553 bp |
| RefSeq ORF | 2823 bp |
| Locus ID | 338523 |
| UniProt ID | Q3UWM4 |
| Gene Summary | Histone demethylase required for brain development. Specifically demethylates dimethylated 'Lys-9' and 'Lys-27' (H3K9me2 and H3K27me2, respectively) of histone H3 and monomethylated histone H4 'Lys-20' residue (H4K20Me1), thereby playing a central role in histone code. Specifically binds trimethylated 'Lys-4' of histone H3 (H3K4me3), affecting histone demethylase specificity: in presence of H3K4me3, it has no demethylase activity toward H3K9me2, while it has high activity toward H3K27me2. Demethylates H3K9me2 in absence of H3K4me3. Has activity toward H4K20Me1 only when nucleosome is used as a substrate and when not histone octamer is used as substrate (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG221543 | Jhdm1d (tGFP-tagged) - Mouse jumonji C domain-containing histone demethylase 1 homolog D (S. cerevisiae) (Jhdm1d), (10ug) |
CNY 7,890.00 |
|
| MR221543 | Jhdm1d (Myc-DDK-tagged) - Mouse jumonji C domain-containing histone demethylase 1 homolog D (S. cerevisiae) (Jhdm1d) |
CNY 6,520.00 |
|
| MR221543L3 | Lenti ORF clone of Jhdm1d (Myc-DDK-tagged) - Mouse jumonji C domain-containing histone demethylase 1 homolog D (S. cerevisiae) (Jhdm1d) |
CNY 9,030.00 |
|
| MR221543L4 | Lenti ORF clone of Jhdm1d (mGFP-tagged) - Mouse jumonji C domain-containing histone demethylase 1 homolog D (S. cerevisiae) (Jhdm1d) |
CNY 9,030.00 |
