Cul4b (NM_028288) Mouse Untagged Clone

CAT#: MC222908

Cul4b (untagged) - Mouse cullin 4B (Cul4b), transcript variant 1, (10ug)



  "NM_028288" in other vectors (4)

CNY 12,540.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
Cul4b Antibody - C-terminal region
    • 50 ug

CNY 4,628.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 2700050M05Rik; AA409770; CUL-4B; mKIAA0695
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC222908 representing NM_028288
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCACGTTCAACTAGGTCTAAAGAAAGAAGAGAGAATGACACCGACTCAGAAGATAACAGTTCGGAAA
CCTCCAATCAAGAGCGCCGAAGGTGTCGCCAAGGTCCGCCCCGCCCTCCCTATCCACCTCTTTTACCCCC
CGTGTTCCCTCCCCCAACCCCCCCACCTCAAGTCCGGCGGACCCGGGGGCTTCAGGATCTGGGAGCTATG
AAAAGTGTCTGCCCAGGCACTTCCGGTTTTTCTTCCCCCAATCCCTCTGCTGCTTCTGCTGCTGCTCAGG
AGGTCAGATCTGCCACTGATGGTAATACCAGCACCACTCCGCCCACCTCTGCCAAGAAGAGAAAGTTAAA
CAGCAGCAGTAGCAGCAGTAACAGTAGTAACGAGAGAGAAGACTTTGATTCCACCTCTTCCTCTTCCACC
CCTCCACAACCCAGAGATTCGGCGTCCCCTTCAACCTCGTCCTTCTGCCTTGGGGTTCCAGTGGCCACTT
CCAGCCACGTACCTATACAGAAGAAGCTGCGGTTTGAAGACACCCTGGAGTTTGTAGGGATTGATACGAA
AATGGCTGAGGAATCTTCCTCCTCATCTTCCTCATCTTCGCCAACTGCTGCAACCTCACAGCAGCAGCAG
CAGCAACAACTTAAAACTAAGAGTATATTAATTTCTTCTGTGGCTTCAGTGCACCATGCAAACGGCCTGG
CTAAATCTTCTACCGCGGTCTCTAGCTTTGCTAACAGCAAGCCCGGCTCAGCTAAGAAGTTAGTGATCAA
GAACTTTAAAGATAAGCCTAAATTACCAGAAAACTATACGGATGAGACATGGCAAAAGCTAAAAGAAGCA
GTGGAAGCCATTCAGAATAGTACTTCAATTAAGTACAATTTAGAAGAACTCTACCAGGCTGTAGAAAATC
TTTGTTCTCATAAGATTTCTGCAAATTTGTATAAGCAGCTGAGGCAGATATGTGAAGATCACATCAAAGC
ACAGATTCATCAGTTCAGAGAGGATTCATTGGATAGTGTTCTTTTTCTCAAAAAGATCGATAGATGCTGG
CAAAACCACTGTAGGCAAATGATAATGATCAGGAGCATTTTTTTGTTTCTGGATAGAACTTATGTTCTTC
AGAATTCAATGCTACCCTCCATTTGGGACATGGGATTAGAGTTATTTAGGGCTCATATTATAAGTGATCA
AAAAGTACAGACAAAAACGATTGATGGCATCCTTCTCTTGATTGAGAGAGAGCGGAATGGTGAAGCAATT
GATAGAAGTTTACTTCGAAGCCTTTTAAGTATGCTGTCTGATTTGCAAATTTACCAAGATTCTTTTGAAC
AGCAATTTTTGCAAGAAACTAATCGGCTTTACGCAGCCGAAGGTCAGAAGTTAATGCAAGAAAGAGAGGT
TCCTGAGTATCTTCATCATGTTAATAAACGCCTAGAAGAAGAAGCTGACAGACTTATTACTTACTTAGAT
CAGACCACCCAGAAGTCACTAATTGCTTCTGTTGAAAAACAACTTCTAGGTGAACACTTAACGGCAATTC
TTCAAAAAGGTTTAAATAGCCTCCTTGATGAAAACCGAATTCAAGATTTGTCTCTCCTGTATCAGCTCTT
CAGTAGAGTTCGGGGTGGAGTTCAGGTTCTCTTACAGCAGTGGATTGAGTATATCAAGGCTTTTGGAAGT
ACCATTGTTATTAATCCTGAAAAAGATAAAACTATGGTTCAAGAATTATTGGACTTTAAAGATAAAGTTG
ACCATATAATTGATACCTGCTTTCTGAAAAATGAAAAATTTATCAATGCCATGAAAGAAGCTTTTGAAAC
ATTCATTAATAAAAGACCAAATAAGCCAGCTGAACTGATAGCTAAGTATGTGGATTCAAAACTTCGTGCA
GGCAACAAAGAAGCTACAGATGAAGAGCTTGAGAAAATGTTGGATAAAATAATGATTATATTTAGATTTA
TCTATGGTAAAGATGTTTTTGAGGCCTTCTATAAGAAAGATTTAGCCAAACGCCTATTAGTTGGCAAGAG
TGCATCAGTAGATGCTGAAAAATCAATGCTGTCCAAACTGAAACACGAATGTGGAGCCGCTTTTACCAGC
AAGCTTGAAGGAATGTTCAAAGACATGGAGCTTTCTAAAGATATCATGATTCAGTTTAAACAGTATATGC
AGAACCAGAATGTACCTGGCAATATTGAATTAACTGTTAATATCCTGACAATGGGTTACTGGCCAACATA
TGTGCCTATGGAAGTCCATTTGCCGCCAGAGATGGTAAAGCTTCAGGAAATTTTCAAGACATTTTACTTA
GGCAAGCACAGTGGCAGGAAACTCCAGTGGCAGTCAACCTTGGGACACTGTGTGCTAAAAGCTGAATTTA
AAGAGGGCAAAAAAGAACTTCAGGTCTCTCTTTTTCAAACCATGGTGCTGCTAATGTTTAATGAAGGAGA
AGAATTCAGTTTAGAAGAGATCAAGCATGCTACTGGGATAGAGGATGGAGAATTAAGAAGAACACTGCAG
TCACTAGCCTGTGGCAAAGCTAGAGTTCTGGCTAAAAATCCTAAGGGCAAAGATATTGAAGATGGTGACA
AATTTATTTGTAATGACGATTTCAAACACAAACTATTCAGGATAAAGATCAATCAAATCCAGATGAAAGA
AACGGTTGAAGAACAAGCAAGCACTACAGAAAGAGTATTTCAAGACAGACAGTACCAAATTGATGCTGCG
ATCGTTCGAATTATGAAGATGAGAAAAACACTTAGCCACAATCTTCTTGTTTCAGAAGTCTACAATCAGC
TAAAATTTCCAGTTAAACCTGCTGATCTTAAGAAGAGAATAGAATCCCTCATTGACCGGGACTACATGGA
AAGAGATAAAGAAAATCCAAATCAGTACAACTATATTGCATAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_028288
Insert Size 2913 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_028288.5, NP_082564.3
RefSeq Size 5020 bp
RefSeq ORF 2913 bp
Locus ID 72584
UniProt ID A2A432
Gene Summary Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition subunit. CUL4B may act within the complex as a scaffold protein, contributing to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Plays a role as part of the E3 ubiquitin-protein ligase complex in polyubiquitination of CDT1, histone H2A, histone H3 and histone H4 in response to radiation-induced DNA damage. Targeted to UV damaged chromatin by DDB2 and may be important for DNA repair and DNA replication. Required for ubiquitination of cyclin E, and consequently, normal G1 cell cycle progression. Regulates the mammalian target-of-rapamycin (mTOR) pathway involved in control of cell growth, size and metabolism. Specific CUL4B regulation of the mTORC1-mediated pathway is dependent upon 26S proteasome function and requires interaction between CUL4B and MLST8 (By similarity). With CUL4A, contributes to ribosome biogenesis (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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