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Atp2a2 (NM_009722) Mouse Untagged Clone
CAT＃: MC223036
Atp2a2 (untagged) - Mouse ATPase, Ca++ transporting, cardiac muscle, slow twitch 2 (Atp2a2), transcript variant 2, (10ug)
CNY 10,976.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 9530097L16Rik; D5Wsu150e; mKIAA4195; SERCA2; Serca2a; SERCA2B |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC223036 representing NM_009722
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAACGCTCACACAAAGACCGTGGAGGAGGTGCTGGGCCACTTCGGGGTCAACGAGAGCACGGGGC TGAGCTTGGAGCAGGTCAAGAAGCTCAAGGAGAGATGGGGCTCCAACGAATTGCCGGCTGAAGAAGGAAA AACCTTGCTGGAACTTGTGATTGAGCAGTTTGAAGACTTACTAGTTAGAATTTTACTGCTGGCAGCATGT ATATCTTTCGTTTTGGCTTGGTTCGAGGAAGGGGAAGAAACGATTACAGCCTTTGTAGAGCCGTTTGTAA TTCTGCTTATCTTGGTAGCCAATGCAATCGTGGGTGTGTGGCAGGAAAGAAATGCTGAAAATGCAATAGA AGCTCTTAAGGAATATGAGCCTGAAATGGGCAAAGTGTATCGACAGGACAGAAAGAGTGTGCAACGAATT AAAGCTAAAGACATAGTTCCTGGTGATATAGTGGAAATTGCTGTTGGTGACAAAGTTCCTGCTGATATTA GATTGACATCCATCAAGTCTACAACTCTAAGAGTCGACCAGTCAATTCTTACAGGTGAATCTGTCTCCGT CATCAAGCATACTGACCCTGTCCCTGACCCCCGAGCTGTTAATCAAGACAAAAAGAACATGCTCTTTTCT GGTACAAACATTGCTGCTGGGAAAGCTATGGGAGTGGTGGTGGCAACTGGAGTTAATACTGAGATCGGCA AGATCCGGGATGAAATGGTGGCAACAGAACAGGAGAGAACACCCCTACAGCAGAAGCTAGACGAGTTTGG GGAGCAGCTTTCCAAAGTTATCTCCCTCATTTGCATTGCAGTCTGGATCATCAACATTGGGCATTTCAAT GACCCAGTTCATGGTGGCTCCTGGATCAGGGGTGCCATCTACTACTTTAAGATTGCCGTGGCCCTGGCTG TTGCCGCAATCCCTGAGGGTCTGCCTGCTGTCATCACCACCTGCTTAGCTCTTGGAACTCGTAGGATGGC AAAGAAAAATGCTATCGTTCGAAGTCTGCCTTCTGTGGAGACCCTTGGTTGTACTTCTGTTATCTGCTCA GATAAGACAGGCACACTTACCACAAACCAGATGTCCGTGTGCAGGATGTTCATTCTGGACAAAGTAGAAG GTGACACTTGTTCCCTTAATGAGTTCAGCATAACTGGATCCACATATGCACCAATTGGAGAAGTGCAAAA GGATGATAAGCCAGTGAAGTGCCATCAGTATGACGGGCTTGTAGAGTTAGCCACCATCTGTGCTCTGTGT AATGACTCTGCTTTGGATTATAATGAGGCAAAGGGTGTGTATGAGAAAGTTGGAGAAGCTACCGAGACTG CTCTCACGTGCCTGGTGGAGAAGATGAATGTATTTGATACTGAGCTGAAGGGGCTTTCTAAAATAGAGCG TGCAAACGCCTGCAACTCGGTCATAAAGCAGCTGATGAAGAAGGAGTTCACTCTGGAGTTTTCACGGGAT AGAAAATCAATGTCCGTCTATTGTACCCCAAACAAGCCAAGCCGGACATCCATGAGCAAGATGTTTGTGA AGGGGGCTCCAGAAGGTGTCATCGATAGGTGCACCCACATCCGAGTTGGAAGTACCAAGGTCCCCATGAC TCCTGGTGTCAAACAGAAGATTATGTCTGTCATTCGGGAGTGGGGCAGTGGCAGCGACACGCTACGGTGC CTGGCTCTGGCCACTCATGACAACCCACTGAAGAGAGAGGAGATGCACCTGGAAGACTCTGCTAACTTCA TCAAATACGAGACCAACCTGACTTTCGTCGGCTGTGTGGGCATGCTGGATCCTCCCAGGATTGAAGTAGC CTCTTCTGTGAAGCTGTGCCGGCAAGCAGGCATCCGGGTCATCATGATCACTGGAGACAACAAAGGCACC GCTGTGGCCATCTGTCGCCGCATTGGCATCTTTGGGCAGGATGAGGATGTGACATCAAAGGCTTTTACAG GGCGAGAGTTTGATGAATTAAGCCCTTCAGCCCAGAGAGATGCCTGCTTAAATGCCCGCTGTTTTGCTCG AGTTGAACCTTCCCACAAGTCTAAGATTGTTGAGTTCCTTCAGTCCTTTGATGAGATCACAGCTATGACT GGTGATGGTGTGAATGATGCTCCTGCTCTGAAGAAATCTGAAATCGGGATTGCCATGGGCTCAGGGACTG CAGTGGCTAAGACTGCTTCTGAGATGGTCCTGGCAGATGACAACTTCTCCACCATTGTGGCTGCTGTTGA GGAGGGGCGAGCCATCTACAACAACATGAAGCAGTTCATCCGCTACCTCATCTCATCCAACGTGGGGGAA GTGGTCTGTATCTTCCTGACGGCAGCCCTTGGGTTTCCTGAGGCTTTAATTCCTGTCCAGTTACTCTGGG TCAATCTGGTGACAGATGGTCTGCCTGCCACTGCGCTGGGGTTCAATCCTCCAGACCTGGACATCATGAA CAAACCCCCCCGGAACCCAAAAGAACCACTGATCAGCGGGTGGCTCTTTTTCCGTTACCTGGCTATTGGC TGTTATGTTGGCGCTGCCACCGTGGGTGCTGCTGCATGGTGGTTCATCGCTGCTGACGGCGGTCCAAGAG TCTCCTTCTACCAGCTGAGTCATTTCCTACAGTGTAAGGAGGACAACCCAGACTTCGATGGAGTGGATTG TGCAATCTTTGAGTCCCCATATCCAATGACAATGGCACTTTCTGTTCTAGTAACCATAGAGATGTGTAAT GCCCTCAACAGCTTGTCTGAAAACCAGTCTTTGCTGAGGATGCCCCCCTGGGAGAATATCTGGCTCGTGG GCTCCATCTGCTTGTCCATGTCACTTCACTTCTTGATCCTCTACGTGGAACCTTTGCCGCTCATTTTCCA GATCACACCGCTGAATCTGACCCAGTGGCTGATGGTGCTGAAAATCTCCTTGCCTGTGATCCTCATGGAT GAGACGCTCAAGTTTGTGGCCCGAAACTACCTGGAACAACCCGCAATACTGGAGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_009722 |
| Insert Size | 2997 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_009722.3, NP_033852.1 |
| RefSeq Size | 4353 bp |
| RefSeq ORF | 2997 bp |
| Locus ID | 11938 |
| UniProt ID | O55143 |
| Gene Summary | This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Isoform SERCA2A is involved in the regulation of the contraction/relaxation cycle. Acts as a regulator of TNFSF11-mediated Ca(2+) signaling pathways via its interaction with TMEM64 which is critical for the TNFSF11-induced CREB1 activation and mitochondrial ROS generation necessary for proper osteoclast generation. Association between TMEM64 and SERCA2 in the ER leads to cytosolic Ca (2+) spiking for activation of NFATC1 and production of mitochondrial ROS, thereby triggering Ca (2+) signaling cascades that promote osteoclast differentiation and activation (PubMed:23395171).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 3' coding region and 3' UTR, compared to variant 1. The resulting isoform (a), also known as SERCA2A, has a shorter and distinct C-terminus compared to isoform b. |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG226794 | Atp2a2 (tGFP-tagged) - Mouse ATPase Ca++ transporting cardiac muscle slow twitch 2 (Atp2a2) transcript variant 2, (10ug) |
CNY 12,560.00 |
|
| MR226794 | Atp2a2 (Myc-DDK-tagged) - Mouse ATPase, Ca++ transporting, cardiac muscle, slow twitch 2 (Atp2a2), transcript variant 2 |
CNY 10,960.00 |
|
| MR226794L3 | Lenti ORF clone of Atp2a2 (Myc-DDK-tagged) - Mouse ATPase, Ca++ transporting, cardiac muscle, slow twitch 2 (Atp2a2), transcript variant 2 |
CNY 11,880.00 |
|
| MR226794L4 | Lenti ORF clone of Atp2a2 (mGFP-tagged) - Mouse ATPase, Ca++ transporting, cardiac muscle, slow twitch 2 (Atp2a2), transcript variant 2 |
CNY 11,880.00 |
