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Mprip (NM_012027) Mouse Untagged Clone
CAT＃: MC223236
Mprip (untagged) - Mouse myosin phosphatase Rho interacting protein (Mprip), transcript variant 2, (10ug)
CNY 13,210.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 9530046C02; AA536749; AI647711; C76423; mKIAA0864; p116Rip; Rhoip3; RIP3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC223236 representing NM_012027
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGCGGCCAAGGAAAACCCGTGCAGGAAATTTCAGGCCAACATCTTCAACAAGAGCAAGTGTCAGA ACTGCTTCAAGCCCCGCGAGTCGCATCTGCTCAACGACGAGGACCTGACGCAGGCAAAACCCATTTATGG TGGCTGGCTGCTCCTGGCTCCAGATGGCACTGACTTTGACAACCCAGTGCACCGGTCACGGAAATGGCAG CGACGATTCTTCATCCTTTATGAGCATGGCCTCTTGCGATATGCCCTGGATGAGATGCCCACCACCCTCC CTCAGGGCACCATCAACATGAACCAGTGCACAGATGTGGTGGATGGGGAGGCCCGCACAGGGCAGAAGTT CTCCCTCTGCATCCTGACACCTGACAAGGAACATTTTATCAGGGCAGAAACCAAGGAGATCATCAGTGGG TGGTTAGAGATGCTCATGGTGTATCCTCGAACCAACAAGCAGAACCAGAAGAAGAAACGGAAAGTAGAGC CACCTACCCCACAGGAGCCTGGGCCTGCTAAGATGGCTGTCACTAGCAGTAGTGGCGGCAGCAGTGGTAG CAGCAGCAGCATTCCCAGTGCTGAGAAAGTTCCCACCACCAAATCCACACTCTGGCAGGAAGAAATGAGA GCCAAAGACCAACCTGATGGGACCAGCCTGAGTCCAGCTCAAAGTCCCAGCCAAAGCCAGCCTCCTGCTG CTTGCACCCCACGGGAACCAGGACTAGAAAGCAAAGAAGATGAAAGCACCATAAGTGGGGACCGTGTGGA TGGTGGTCGGAAAGTACGGGTAGAGAGCGGCTACTTCTCCCTGGAGAAGGCCAAGCAGGACCTGAGGGCT GAGGAGCAGCTGCCCCCACTACTCTCCCCACCCAGCCCCAGCACCCCACACAGCAGGAGGTCCCAGGTAA TTGAGAAATTTGAGGCCTTGGACATTGAAAAAGCAGAGCACATGGAAACCAACATGCTGATTCTGACCAC TCCATCAAGTGACACTCGTCAGGGCCGCAGTGAGAGACGGGCCATCCCTAGAAAGCGGGACTTTGCCAGC GAAGCCCCCACAGCTCCTCTCTCAGATGCTTGTCCCCTGTCTCCACACCGAAGAGCCAAGTCATTGGACA GGAGGTCCACAGAATCCTCCATGACGCCTGACCTGCTGAATTTCAAGAAAGGCTGGCTAACCAAGCAGTA TGAGGATGGCCAGTGGAAGAAACACTGGTTTGTCCTGGCAGATCAGAGCCTGAGATACTACAGGGATTCC GTGGCTGAGGAGGCAGCTGACCTAGATGGAGAAATTAACTTGTCCACTTGCTATGATGTCACTGAGTATC CAGTCCAGAGAAACTATGGCTTTCAGATACATACCAAGGAGGGTGAGTTCACCCTCTCAGCCATGACATC TGGCATACGACGGAACTGGATCCAGACCATTATGAAACATGTGCTCCCAGCATCTGCCCCAGATGTGACC AGCTCATTGCCTGAGGGAAAGAATAAAAGCACATCTTTTGAGACCTGCTCAAGGTCAACTGAGAAGCAAG AAGCTGAGCCAGGAGAGCCAGATCCTGAACAAAAGAAGAGCCGTGCTCGAGAGCGGAGGCGGGAGGGTCG CTCCAAGACCTTTGACTGGGCTGAATTCCGCCCTATACAACAGGCCCTGGCTCAAGAAAGGGCCAGCGCT GTTGGGTCTTCTGATTCTGGTGACCCTGGGTGTCTTGAAGCCGAGCCAGGTGAGCTGGAGCGTGAACGAG CTCGACGTCGAGAAGAACGCCGCAAGCGCTTTGGGATGCTGGACACCATTGATGGACCAGGAATGGAGGA CACAGCCCTACGTATGGACATCGACCGGAGCCCAGGGCTGCTGGGAACCCCTGACCTCAAGACACAGAAT GTCCACGTGGAAATTGAGCAGCGTTGGCATCAAGTGGAGACCACCCCTCTCAGGGAGGAGAAACAGGTGC CCATTGCCCCTCTGCACCTATCCTTGGAGGACAGAAGTGAGCGGCTCTCCACACATGAGCTCACCTCTCT GCTAGAGAAGGAGCTGGAGCAGAGCCAGAAAGAAGCCTCGGATCTTCTAGAGCAGAACCGGCTTCTACAG GACCAGTTAAGGGTGGCCCTGGGCAGAGAACAGAGTGCCCGGGAGGGCTATGTGCTGCAGGCTACATGTG AGCGAGGCTTTGCCGCCATGGAGGAGACACATCAGAAGAAGATTGAAGACCTACAGAGGCAACACCAGCG GGAGCTAGAGAAGCTGCGGGAGGAAAAAGACCGCCTCCTGGCTGAGGAGACAGCTGCCACCATATCAGCC ATTGAAGCCATGAAGAATGCTCACCGAGAGGAGATGGAACGAGAGTTAGAGAAGAGCCAGCGGTCTCAGA TCAGCAGCATCAATTCAGACATAGAAGCCCTGAGGCGACAGTACCTGGAGGAGCTGCAGTCAGTGCAGCG GGAGCTGGAAGTCCTTTCAGAGCAGTACTCCCAAAAGTGCCTGGAGAATGCACACCTAGCCCAGGCGCTG GAGGCCGAGCGTCAGGCCCTGCGGCAATGCCAGCGTGAGAACCAGGAGCTCAACGCCCACAACCAGGAGC TGAATAACCGCCTGGCTGCGGAGATCACACGGTTGCGGACACTACTGACTGGAGACGGTGGTGGGGAGTC CACTGGGTTGCCTCTCACACAGGGCAAGGATGCCTATGAGTTAGAGGTCTTGTTGCGGGTCAAAGAGTCA GAAATCCAGTACCTGAAACAGGAGATCAGCTCCCTCAAGGATGAACTCCAGACAGCCCTGCGGGATAAGA AGTATGCTAGTGACAAGTACAAAGACATCTACACTGAGCTCAGCATTGCCAAGGCCAAGGCTGACTGCGA CATCAGCAGGCTGAAGGAGCAGCTGAAAGCAGCCACAGAGGCACTGGGCGAGAAATCTCCTGAAGGCACT ACTGTGTCAGGATATGACATAATGAAATCTAAAAGCAATCCTGACTTCCTGAAGAAAGACAGATCCTGTG TTACCCGGCAACTCAGAAACATCAGGTCCAAGTCCGTAATTGAGCAGGTCTCATGGGATAACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_012027 |
| Insert Size | 3075 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_012027.2, NP_036157.2 |
| RefSeq Size | 8784 bp |
| RefSeq ORF | 3075 bp |
| Locus ID | 26936 |
| UniProt ID | P97434 |
| Gene Summary | Targets myosin phosphatase to the actin cytoskeleton. Required for the regulation of the actin cytoskeleton by RhoA and ROCK1. Depletion leads to an increased number of stress fibers in smooth muscle cells through stabilization of actin fibers by phosphorylated myosin. Overexpression of MRIP as well as its F-actin-binding region leads to disassembly of stress fibers in neuronal cells.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) has an additional exon in the 3' coding region that causes a frameshift, compared to variant 1. The encoded protein (isoform 2) has a shorter and distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG224193 | Mprip (tGFP-tagged) - Mouse myosin phosphatase Rho interacting protein (Mprip) transcript variant 2, (10ug) |
CNY 11,310.00 |
|
| MR224193 | Mprip (Myc-DDK-tagged) - Mouse myosin phosphatase Rho interacting protein (Mprip), transcript variant 2 |
CNY 10,260.00 |
|
| MR224193L3 | Lenti ORF clone of Mprip (Myc-DDK-tagged) - Mouse myosin phosphatase Rho interacting protein (Mprip), transcript variant 2 |
CNY 12,160.00 |
|
| MR224193L4 | Lenti ORF clone of Mprip (mGFP-tagged) - Mouse myosin phosphatase Rho interacting protein (Mprip), transcript variant 2 |
CNY 12,160.00 |
