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Tlr9 (NM_031178) Mouse Untagged Clone
CAT＃: MC223287
Tlr9 (untagged) - Mouse toll-like receptor 9 (Tlr9), (10ug)
CNY 10,792.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC223287 representing NM_031178
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTTCTCCGTCGAAGGACTCTGCACCCCTTGTCCCTCCTGGTACAGGCTGCAGTGCTGGCTGAGACTC TGGCCCTGGGTACCCTGCCTGCCTTCCTACCCTGTGAGCTGAAGCCTCATGGCCTGGTGGACTGCAATTG GCTGTTCCTGAAGTCTGTACCCCGTTTCTCTGCGGCAGCATCCTGCTCCAACATCACCCGCCTCTCCTTG ATCTCCAACCGTATCCACCACCTGCACAACTCTGACTTCGTCCACCTGTCCAACCTGCGGCAGCTGAACC TCAAGTGGAACTGTCCACCCACTGGCCTTAGCCCCCTGCACTTCTCTTGCCACATGACCATTGAGCCCAG AACCTTCCTGGCTATGCGTACACTGGAGGAGCTGAACTTGAGCTATAATGGTATCACCACTGTGCCCCGA CTGCCCAGCTCCCTGGTGAATCTGAGCCTGAGCCACACCAACATCCTGGTTCTAGATGCTAACAGCCTCG CCGGCCTATACAGCCTGCGCGTTCTCTTCATGGACGGGAACTGCTACTACAAGAACCCCTGCACAGGAGC GGTGAAGGTGACCCCAGGCGCCCTCCTGGGCCTGAGCAATCTCACCCATCTGTCTCTGAAGTATAACAAC CTCACAAAGGTGCCCCGCCAACTGCCCCCCAGCCTGGAGTACCTCCTGGTGTCCTATAACCTCATTGTCA AGCTGGGGCCTGAAGACCTGGCCAATCTGACCTCCCTTCGAGTACTTGATGTGGGTGGGAATTGCCGTCG CTGTGACCATGCCCCCAATCCCTGTATAGAATGTGGCCAAAAGTCCCTCCACCTGCACCCTGAGACCTTC CATCACCTGAGCCATCTGGAAGGCCTGGTGCTGAAGGACAGCTCTCTCCATACACTGAACTCTTCCTGGT TCCAAGGTCTGGTCAACCTCTCGGTGCTGGACCTAAGCGAGAACTTTCTCTATGAAAGCATCACCCACAC CAATGCCTTTCAGAACCTAACCCGCCTGCGCAAGCTCAACCTGTCCTTCAATTACCGCAAGAAGGTATCC TTTGCCCGCCTCCACCTGGCAAGTTCCTTTAAGAACCTGGTGTCACTGCAGGAGCTGAACATGAACGGCA TCTTCTTCCGCTTGCTCAACAAGTACACGCTCAGATGGCTGGCCGATCTGCCCAAACTCCACACTCTGCA TCTTCAAATGAACTTCATCAACCAGGCACAGCTCAGCATCTTTGGTACCTTCCGAGCCCTTCGCTTTGTG GACTTGTCAGACAATCGCATCAGTGGGCCTTCAACGCTGTCAGAAGCCACCCCTGAAGAGGCAGATGATG CAGAGCAGGAGGAGCTGTTGTCTGCGGATCCTCACCCAGCTCCGCTGAGCACCCCTGCTTCTAAGAACTT CATGGACAGGTGTAAGAACTTCAAGTTCACCATGGACCTGTCTCGGAACAACCTGGTGACTATCAAGCCA GAGATGTTTGTCAATCTCTCACGCCTCCAGTGTCTTAGCCTGAGCCACAACTCCATTGCACAGGCTGTCA ATGGCTCTCAGTTCCTGCCGCTGACTAATCTGCAGGTGCTGGACCTGTCCCATAACAAACTGGACTTGTA CCACTGGAAATCGTTCAGTGAGCTACCACAGTTGCAGGCCCTGGACCTGAGCTACAACAGCCAGCCCTTT AGCATGAAGGGTATAGGCCACAATTTCAGTTTTGTGACCCATCTGTCCATGCTACAGAGCCTTAGCCTGG CACACAATGACATTCATACCCGTGTGTCCTCACATCTCAACAGCAACTCAGTGAGGTTTCTTGACTTCAG CGGCAACGGTATGGGCCGCATGTGGGATGAGGGGGGCCTTTATCTCCATTTCTTCCAAGGCCTGAGTGGC CTGCTGAAGCTGGACCTGTCTCAAAATAACCTGCATATCCTCCGGCCCCAGAACCTTGACAACCTCCCCA AGAGCCTGAAGCTGCTGAGCCTCCGAGACAACTACCTATCTTTCTTTAACTGGACCAGTCTGTCCTTCCT ACCCAACCTGGAAGTCCTAGACCTGGCAGGCAACCAGCTAAAGGCCCTGACCAATGGCACCCTGCCTAAT GGCACCCTCCTCCAGAAACTCGATGTCAGTAGCAACAGTATCGTCTCTGTGGTCCCAGCCTTCTTCGCTC TGGCGGTCGAGCTGAAAGAGGTCAACCTCAGCCACAACATTCTCAAGACGGTGGATCGCTCCTGGTTTGG GCCCATTGTGATGAACCTGACAGTTCTAGACGTGAGAAGCAACCCTCTGCACTGTGCCTGTGGGGCAGCC TTCGTAGACTTACTGTTGGAGGTGCAGACCAAGGTGCCTGGCCTGGCTAATGGTGTGAAGTGTGGCAGCC CCGGCCAGCTGCAGGGCCGTAGCATCTTCGCGCAGGACCTGCGGCTGTGCCTGGATGAGGTCCTCTCTTG GGACTGCTTTGGCCTTTCACTCTTGGCTGTGGCCGTGGGCATGGTGGTGCCTATACTGCACCATCTCTGC GGCTGGGACGTCTGGTACTGTTTTCATCTGTGCCTGGCATGGCTACCTTTGCTAGCCCGCAGCCGACGCA GCGCCCAAACTCTCCCTTATGATGCCTTCGTGGTGTTCGATAAGGCACAGAGCGCAGTTGCCGACTGGGT GTATAACGAGCTGCGGGTGCGGCTGGAGGAGCGGCGCGGCCGCCGAGCCCTACGCTTGTGTCTGGAGGAC CGAGATTGGCTGCCTGGCCAGACGCTCTTCGAGAACCTCTGGGCTTCCATCTATGGGAGCCGCAAGACTC TATTTGTGCTGGCCCACACGGACCGCGTCAGTGGCCTCCTGCGCACCAGCTTCCTGCTGGCTCAGCAGCG CCTGTTGGAAGACCGCAAGGACGTGGTGGTGTTGGTGATCCTGCGTCCGGATGCCCACCGCTCCCGCTAT GTGCGACTGCGCCAGCGTCTCTGCCGCCAGAGTGTGCTCTTCTGGCCCCAGCAGCCCAACGGGCAGGGGG GCTTCTGGGCCCAGCTGAGTACAGCCCTGACTAGGGACAACCGCCACTTCTATAACCAGAACTTCTGCCG GGGACCTACAGCAGAATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_031178 |
| Insert Size | 3099 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_031178.2, NP_112455.2 |
| RefSeq Size | 3471 bp |
| RefSeq ORF | 3099 bp |
| Locus ID | 81897 |
| UniProt ID | Q9EQU3 |
| Gene Summary | Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:18931679, PubMed:21402738, PubMed:14993594, PubMed:17474149, PubMed:25686612, PubMed:18820679). Plays a role in defense against systemic mouse cytomegalovirus infection (PubMed:14993594). Controls lymphocyte response to Helicobacter infection (PubMed:17474149). Upon CpG stimulation, induces B-cell proliferation, activation, survival and antibody production (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG227011 | Tlr9 (tGFP-tagged) - Mouse toll-like receptor 9 (Tlr9), (10ug) |
CNY 12,376.00 |
|
| MR227011 | Tlr9 (Myc-DDK-tagged) - Mouse toll-like receptor 9 (Tlr9) |
CNY 10,776.00 |
|
| MR227011L3 | Lenti ORF clone of Tlr9 (Myc-DDK-tagged) - Mouse toll-like receptor 9 (Tlr9) |
CNY 12,160.00 |
|
| MR227011L4 | Lenti ORF clone of Tlr9 (mGFP-tagged) - Mouse toll-like receptor 9 (Tlr9) |
CNY 13,176.00 |
