Nlrp3 (NM_145827) Mouse Untagged Clone

CAT#: MC223293

Nlrp3 (untagged) - Mouse NLR family, pyrin domain containing 3 (Nlrp3), (10ug)



  "NM_145827" in other vectors (4)

CNY 13,300.00


货期*
7周

规格
    • 10 ug

Product images

经常一起买 (3)
NLRP3 Rabbit pAb
    • 100 ul

CNY 2,640.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms AGTAVPRL; AII/AVP; Cias1; FCAS; FCU; Mmig1; MWS; NALP3; Pypaf1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC223293 representing NM_145827
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACGAGTGTCCGTTGCAAGCTGGCTCAGTATCTAGAGGACCTTGAAGATGTGGACCTCAAGAAATTCA
AAATGCATTTGGAAGATTACCCGCCCGAGAAAGGCTGTATCCCAGTCCCCAGGGGCCAGATGGAGAAGGC
AGATCACTTGGATCTAGCCACACTCATGATTGACTTCAATGGCGAGGAGAAGGCCTGGGCCATGGCTGTG
TGGATCTTTGCTGCGATCAACAGGCGAGACCTCTGGGAAAAAGCTAAGAAGGACCAGCCAGAGTGGAATG
ACACGTGTACATCACATTCCTCTATGGTATGCCAGGAGGACAGCCTTGAAGAAGAGTGGATGGGTTTGCT
GGGATATCTCTCCCGCATCTCCATTTGTAAAAAGAAGAAAGATTACTGTAAGATGTACAGACGACATGTG
AGAAGCAGGTTCTACTCTATCAAGGACAGGAACGCGCGTCTAGGTGAGAGTGTGGACCTCAACAGTCGCT
ACACGCAGCTCCAACTGGTCAAGGAGCATCCAAGCAAGCAGGAGCGGGAGCATGAACTCCTGACCATCGG
CCGGACTAAAATGCGGGACAGCCCCATGAGTTCCCTTAAGCTGGAGCTGCTGTTTGAGCCCGAGGACGGG
CACTCGGAGCCTGTGCACACAGTGGTGTTCCAGGGAGCAGCAGGCATCGGGAAAACCATCCTAGCCAGGA
AGATTATGTTGGACTGGGCACTGGGAAAGCTCTTCAAAGACAAATTTGACTATTTGTTCTTTATCCACTG
CCGAGAGGTGAGCCTCAGGACGCCAAGGAGTCTAGCAGACCTGATTGTCAGCTGCTGGCCTGACCCAAAC
CCACCAGTGTGCAAGATCCTGCGCAAGCCTTCCAGGATCCTCTTCCTCATGGATGGCTTTGATGAGCTAC
AAGGGGCCTTTGACGAGCACATTGGGGAGGTCTGCACAGACTGGCAAAAGGCTGTGCGGGGAGACATTCT
GCTAAGCAGCCTCATCCGAAAGAAACTGCTGCCCAAGGCCTCTCTGCTCATAACGACGAGGCCGGTAGCC
TTGGAGAAACTGCAGCATCTCCTGGACCACCCCCGCCATGTGGAGATCCTAGGTTTCTCTGAGGCCAAAA
GGAAGGAGTATTTCTTTAAGTATTTCTCCAACGAGCTGCAGGCCCGGGAGGCCTTCAGGCTGATCCAAGA
GAATGAGGTCCTCTTTACCATGTGCTTCATCCCCCTGGTCTGCTGGATTGTGTGCACGGGGCTAAAGCAA
CAGATGGAGACCGGGAAGAGCCTGGCCCAGACCTCCAAGACCACTACGGCCGTCTACGTCTTCTTCCTTT
CCAGCCTGCTGCAATCCCGGGGGGGCATTGAGGAGCATCTCTTCTCTGACTACCTACAGGGGCTCTGTTC
ACTGGCTGCGGATGGAATTTGGAACCAGAAAATCCTATTTGAGGAGTGTGATCTGCGGAAGCACGGCCTG
CAGAAGACTGACGTCTCCGCTTTCCTGAGGATGAACGTGTTCCAGAAGGAAGTGGACTGCGAGAGATTCT
ACAGCTTCAGCCACATGACTTTCCAGGAGTTCTTCGCTGCTATGTACTATTTGCTGGAAGAGGAGGCAGA
GGGGGAGACCGTGAGGAAAGGACCAGGAGGTTGTTCAGATCTTCTGAACCGAGACGTGAAGGTCCTACTA
GAAAATTACGGCAAGTTTGAAAAAGGCTATCTGATTTTTGTTGTCCGATTCCTCTTTGGCCTTGTAAACC
AGGAGAGAACCTCTTATTTGGAGAAGAAACTAAGTTGCAAGATCTCTCAGCAAGTCAGACTGGAACTACT
GAAGTGGATTGAAGTGAAAGCCAAGGCCAAGAAGCTGCAGTGGCAGCCCAGCCAACTGGAACTGTTCTAC
TGCCTGTACGAGATGCAGGAGGAAGACTTTGTGCAGAGTGCCATGGACCACTTTCCCAAAATTGAGATCA
ACCTCTCTACCAGAATGGACCACGTGGTTTCCTCCTTTTGTATTAAGAACTGTCATAGGGTCAAAACGCT
TTCCCTGGGTTTTTTTCACAACTCGCCCAAGGAGGAAGAAGAAGAGAGGAGAGGAGGTCGACCCTTGGAC
CAGGTTCAGTGTGTTTTCCCAGACACTCATGTTGCCTGTTCTTCCAGACTGGTGAACTGCTGCCTCACTT
CTAGCTTCTGCCGTGGTCTCTTCTCAAGTCTAAGCACCAACCGGAGCCTCACTGAACTGGACCTCAGTGA
CAATACTCTGGGAGACCCGGGCATGAGGGTGCTGTGTGAGGCACTCCAGCACCCAGGCTGTAACATTCAG
AGACTGTGGTTGGGGCGCTGCGGACTGTCCCATCAATGCTGCTTCGACATCTCCTCTGTCCTGAGCAGCA
GCCAGAAGCTGGTGGAGCTGGACCTCAGTGACAATGCCCTGGGGGACTTTGGAATCAGATTGCTGTGTGT
GGGACTGAAGCACCTGCTCTGCAACCTCCAGAAACTGTGGTTGGTGAGCTGCTGTCTCACATCCGCGTGT
TGTCAGGATCTCGCATTGGTTCTGAGCTCCAACCATTCTCTGACCAGACTGTACATTGGAGAAAATGCCT
TGGGAGACTCAGGAGTCCAAGTTTTGTGTGAAAAGATGAAGGACCCACAGTGTAACTTGCAGAAGCTGGG
GTTGGTGAATTCCGGCCTTACTTCAATCTGTTGTTCAGCTCTGACCTCTGTGCTCAAAACCAACCAGAAC
TTCACACACCTCTATCTACGAAGCAATGCCCTTGGAGACACAGGACTCAGGCTCCTCTGTGAGGGGCTTC
TGCACCCGGACTGTAAACTACAGATGCTGGAATTAGACAACTGCAGCCTCACCTCACACAGCTGCTGGAA
TCTCTCCACAATTCTGACCCACAACCACAGCCTTCGGAAGCTGAACCTGGGCAACAATGATCTTGGCGAT
CTGTGCGTGGTGACCCTCTGTGAGGTGCTGAAACAGCAGGGCTGCCTCCTGCAGAGCCTACAGTTGGGTG
AAATGTACTTAAATCGTGAAACAAAACGTGCCTTAGAAGCGCTCCAGGAAGAAAAGCCTGAGCTGACTAT
AGTCTTCGAGATTTCCTGGTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_145827
Insert Size 3102 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_145827.3, NP_665826.1
RefSeq Size 4021 bp
RefSeq ORF 3102 bp
Locus ID 216799
UniProt ID Q8R4B8
Gene Summary As the sensor component of the NLRP3 inflammasome, plays a crucial role in innate immunity and inflammation. In response to pathogens and other damage-associated signals, initiates the formation of the inflammasome polymeric complex, made of NLRP3, PYCARD and CASP1 (or possibly CASP4/CASP11). Recruitment of proCASP1 to the inflammasome promotes its activation and CASP1-catalyzed IL1B and IL18 maturation and secretion in the extracellular milieu (PubMed:28847925). Activation of NLRP3 inflammasome is also required for HMGB1 secretion (PubMed:22801494). The active cytokines and HMGB1 stimulate inflammatory responses. Inflammasomes can also induce pyroptosis, an inflammatory form of programmed cell death. Under resting conditions, NLRP3 is autoinhibited. NLRP3 activation stimuli include extracellular ATP, reactive oxygen species, K(+) efflux, crystals of monosodium urate or cholesterol, amyloid-beta fibers, environmental or industrial particles and nanoparticles, cytosolic dsRNA, etc. However, it is unclear what constitutes the direct NLRP3 activator. Activation in presence of cytosolic dsRNA is mediated by DHX33 (By similarity). Independently of inflammasome activation, regulates the differentiation of T helper 2 (Th2) cells and has a role in Th2 cell-dependent asthma and tumor growth. During Th2 differentiation, required for optimal IRF4 binding to IL4 promoter and for IRF4-dependent IL4 transcription. Binds to the consensus DNA sequence 5'-GRRGGNRGAG-3'. May also participate in the transcription of IL5, IL13, GATA3, CCR3, CCR4 and MAF (PubMed:26098997).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (1) represents the longest transcript. Variants 1 and 2 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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