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Ankrd27 (NM_145633) Mouse Untagged Clone
CAT＃: MC223380
Ankrd27 (untagged) - Mouse ankyrin repeat domain 27 (VPS9 domain) (Ankrd27), transcript variant 1, (10ug)
CNY 13,490.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | AA408090; BC016493; D330003H11Rik; Varp |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC223380 representing NM_145633
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCTATATGATGAAGATCTCCTGAAAAACCCTTTCTACCTGGCCCTTCAGAAGTGGCGCCCCGACT TGTGCAGCAAGGTGGCCCAGATCCATGGCATTGTCCTAGTGCCTTGCAGAGGAAGCCTGCCCGGCAGTGT GCAGGCCTCCTGCCAGTTCGAGTCCTATGTTTTGGTACCCACGGAAGGACACTTTCAGACCTTAGATGGA AAGGCTGTCGTCATTGAAGGAAACAGGATTAAGCTAGGAGCAGGTTTTGCTTGCCTTCTCTCTGTGCCCA TCCTCTTTGAGGAGACTTTCTACAACGAGAAAGAGGAGAGTTTCAGCATTCTCTGCATTGCTCATCCTTT GGAGAGGAGAGAGACTTCAGAAGAACCTTCAGCGCCTGCAGATCCTTTCTCCCTGAAAACCATCGAAGAT GTGAGAGAATTTTTGGGAAGACACTCAGAGAAATTTGACAAGAACATTGCCTCTTTCCACCGGACGTTCC GAGAATGTGAAAGAAAGAGCCTCCGCCACCACATAGACTCCGTAAATGCTCTCTACACCAAATGCCTCCA GCAGCTTCTCCGAGACTCTCACCTGAAGGTTCTTGCAAAGCAGGAGGCCCAGATGAACCTGATGAAGCAG GCCGTAGAGATGTATGTCCATCACGATATTTACGACCTGATCTTTAAATACGTGGGGACCATGGAGGCGA GCGAGGATGCCGCCTTTAACAAAATCACGAGAAGCCTTCAAGATCTCCAGCAGAAAGACATCGGCGTGAA ACCCGAGTTCAGCTTCAACATCCCTCGGGCAAAGAGAGAGCTGGGTCAGTTGAACAAGTGTACGTCGCCA CAGCAGAAGCTGCTGTGCCTGAGGAAGGTGGTCCAGCTCATGACACAATCTCCCAGCCAGAGAGTGAACT TGGAGACCATGTGTGCCGATGATCTCCTCTCGGTCCTGTTATATCTGCTCGTGAAGACAGAGATCCCTAA CTGGATGGCAAATTTGAGCTACATCAAAAACTTCAGATTTAGCAGCTCGGCCAAAGATGAGCTAGGATAC TGCCTGACCTCAGTCGAGGCTGCGATTGAATACATCCGGCAAGGAAGTCTCTCCACGAAGACGCCTGACG CTGAGGGATTTGGCGACAGACTATTTCTCAAGCAGCGGATGAACTTGCTGTCCCAGATGACATCAACACC CATCGACTGCCTGTTCAAGCACATCGCCTCTGGTAACCAAAAGGAAGTGGAAAGACTTCTGAGCCAAGAT GACCAGGACAAAGACGCCATGCAAAAGATGTGCCACCCACTTTGTTCCTGTGAGGACTGTGAGAAGCTTA TCTCTGGGAGGCTGAATGACCCGTCAGTTGTCACTCCTTTCTCCAGAGATGACAGAGGTCAAACACCCCT CCATGTGGCTGCCCTCTGTGGTCAGGCATCCCTCATTGACTTCCTGGTCTCCAAAGGTGCCGTAGTGAAC GCCACAGACTATCATGGGTCCACCCCCTTACATCTGGCATGTCAGAAAGGCTTCCAAAGTGTGACGCTGC TGCTGCTGCACTACAAGGCCAGCACTGAGGTTCAGGACAACAACGGCAACACCCCGCTGCACCTGGCCTG CACCTATGGGCAGGAGGATTGCGTGAAGGCCCTGGTCTACTATGATGTCCAGGCCTGCAGATTGGATATT GGTAATGAGAAAGGAGACACAGCCCTGCACATTGCTGCACGCTGGGGTTACGAGGGCATCATAGAGACAC TGCTGCAGAATGGAGCCCCCACAGCAGTCCAGAACAGACTGAAGGAAACACCACTCAAGTGTGCTCTGAA CTCGAAGATTCTGTCTATCATGGAAGCTCATCACCTATCCTCTGACAGGAGGCCAAGGCCTTCTGAGGTT CCTGCACAGTCCCCTACACGCTCTGTGGATTCCATCAGCCAGGGCTCCTCCACCTCCAGCTTTTCTTCCA TATCGGTGAGCTTCAGGCAAGAAGAGGTCAAAAAGGACTACAGGGAGGTAGAAAAGCTTCTAAGGGCAGT TGCTGATGGCGATCTAGAAATGGTACGATACCTTTTGGAGTGGACAGAGGATGACCTAGACGATGTGGAA GACGCCATCAGCACGGTGGATCTCGAATTCTGTCACCCCTTATGCCAGTGTCCCAAGTGTGCTCCAGCTC AGAAACTGGCCAGGATTTCTGCCAATGGGCTTAGTGTGAATGTGACCAACCAGGACGGCTTCTCTCCCCT GCACATGGCTGCCCTGCATGGCCGCACAGACCTCGTCCCCCTCCTGCTGAAGCACGGTGCTTACTCAGGT GCCAGAAACACAAGCCAAGCCGTCCCGCTCCACCTGGCCTGCCAGCAGGGGCACTTTCAGGTGGCAAAAT GTCTCTTAGATTCCAATGCAAAACCTAATAAAAAGGACCTCAGTGGGAACACACCCCTCATTTGTGCCTG CTCTGCCGGCCACCATGAAGTCGCTGCGCTGTTGCTACAGCACGGGGCCTCCATCAATGCTTGCAACAAC AAAGGCAACACGGCCCTGCATGAGGCTGTGATGGGAAGGCACACCCTGGTAGTGGAGCTGCTTCTGTTCT ACGGGGCGTCGGTTGACATCCTGAACAAGAGGCAGTACACGGCGGCGGACTGTGCCGAGCAGGACTCAAA AATAATGGAGTTGCTGCAGGTAGTGCCAGGCTGTGTTGCGTCTCTAGACAGCGTCGAGGAGGCAGACCAC GAGGGTTACGTGACTGTTGAGATCAGGAGAAAATGGAACCCCAAAATGTACAACCTGCCAGAGGAACCTC TTAGGAGACAGTTTTGCCTCATTAACCCGGGGGGTCGGTTCCAGGAGAGGACATCCAGGGAGACTATGGG AAGGGATAGAAGTGTGCCCGATCTTGCAGGACGTTCTCTTCAGGAGCCAGAGAAGCAAAGAGTCACCGGA AAGCAGAGTGACCTGTCAGACCTGAGCAGGTATCAGACCTCTGAGGAAGGAAACAAGGGGCTCCCGGAGA GGCCCGTGTCGAGACAGGCTGCTCCAGGACACCGGCCGATGGTCCGCAGACACACAGTCAATGACGCAGC CATACTCCAGGTCCCAGAGGTGACTGTCCACCTCACCACCCACGAGGCGAGTGTTCCTCAGTCTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_145633 |
| Insert Size | 3147 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_145633.3, NP_663608.3 |
| RefSeq Size | 4292 bp |
| RefSeq ORF | 3147 bp |
| Locus ID | 245886 |
| UniProt ID | Q3UMR0 |
| Gene Summary | May be a guanine exchange factor (GEF) for Rab21, Rab32 and Rab38 and regulate endosome dynamics (By similarity). May regulate the participation of VAMP7 in membrane fusion events; in vitro inhibits VAMP7-mediated SNARE complex formation by trapping VAMP7 in a closed, fusogenically inactive conformation (By similarity). Involved in peripheral melanosomal distribution of TYRP1 in melanocytes; the function, which probably is implicating vesicle-trafficking, includes cooperation with Rab32, Rab38 and VAMP7 (PubMed:19403694, PubMed:21187289). Involved in the regulation of neurite growth; the function seems to require its GEF activity, probably towards Rab21, and VAMP7 but not Rab32/38 (PubMed:19745841, PubMed:22171327). Proposed to be involved in Golgi sorting of VAMP7 and transport of VAMP7 vesicles to the cell surface; the function seems to implicate kinesin heavy chain isoform 5 proteins, GOLGA4, RAB21 and MACF1. Required for the colocalization of VAMP7 and Rab21, probably on TGN sites (By similarity). Involved in GLUT1 endosome-to-plasma membrane trafficking; the function is dependent of association with VPS29 (By similarity). Regulates the proper trafficking of melanogenic enzymes TYR, TYRP1 and DCT/TYRP2 to melanosomes in melanocytes (PubMed:26620560).[UniProtKB/Swiss-Prot Function] |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG218295 | Ankrd27 (tGFP-tagged) - Mouse ankyrin repeat domain 27 (VPS9 domain) (Ankrd27) transcript variant 1, (10ug) |
CNY 11,500.00 |
|
| MR218295 | Ankrd27 (Myc-DDK-tagged) - Mouse ankyrin repeat domain 27 (VPS9 domain) (Ankrd27), transcript variant 1 |
CNY 10,450.00 |
|
| MR218295L3 | Lenti ORF clone of Ankrd27 (Myc-DDK-tagged) - Mouse ankyrin repeat domain 27 (VPS9 domain) (Ankrd27), transcript variant 1 |
CNY 12,350.00 |
|
| MR218295L4 | Lenti ORF clone of Ankrd27 (mGFP-tagged) - Mouse ankyrin repeat domain 27 (VPS9 domain) (Ankrd27), transcript variant 1 |
CNY 12,350.00 |
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