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Sema5a (NM_009154) Mouse Untagged Clone
CAT＃: MC223449
Sema5a (untagged) - Mouse sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (Sema5a), (10ug)
CNY 13,780.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 5930434A13; 9130201M22Rik; AI464145; Semaf; semF |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC223449 representing NM_009154
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGGAGCCTGCATCCTTGCATGGCTGTTCTCAAGCCTGGGAGTGTGGAGACTTGCTAGGCCCGAGA CCCAGGACCCTGCCAAGTGCCAGAGAGCTGAGCACCCTGTCGTCTCTTACAAAGAAATTGGCCCCTGGTT ACGGGAGTTCAGAGCCGAGAATGCTGTGGATTTCTCGAGGTTAACATTTGACCCAGGACAGAAAGAACTT GTCGTAGGAGCGAGAAACTATCTCTTCAGATTAGAGCTTGAGGATCTGTCTCTCATCCAGGCTGTGGAGT GGGAGTGTGACGAAGCCACCAAGAAGGCCTGTTACAGCAAGGGCAAATCGAAGGAGGAATGTCAGAACTA CATACGAGTGCTCTTAGTTGGTGGGGACCGGCTATTCACCTGTGGGACCAATGCCTTCACACCTGTCTGT ACCATCCGCTCGTTAAGTAACCTGACTGAGATCCATGATCAGATCAGTGGCATGGCCCGCTGTCCCTACA GTCCCCAGCACAATTCTACCGCCCTACTCACTGCCAGTGGTGAGCTCTATGCTGCAACAGCCATGGATTT CCCAGGGCGAGATCCAGCCATTTACCGAAGTCTGGGCACTTTGCCTCCTCTTCGAACTGCACAGTACAAC TCCAAATGGCTCAATGAACCAAACTTTGTGTCTTCCTACGACATCGGAAATTTCACCTACTTCTTCTTCC GAGAAAACGCCGTGGAACATGACTGTGGGAAGACGGTGTTCTCCAGGGCTGCCCGGGTCTGTAAAAATGA CATTGGAGGGCGATTTCTTCTGGAGGACACCTGGACTACCTTCATGAAGGCTCGCCTCAACTGCTCCCGG CCTGGTGAGGTGCCCTTCTACTACAATGAGCTGCAGGGTACTTTCTTCCTGCCGGAGCTGGATCTGATCT ATGGTATCTTCACCACCAATGTAAACAGCATCGCTGCCTCTGCTGTCTGTGTCTTCAACCTGAGCGCCAT CTCACAGGCCTTCAATGGACCCTTCAAGTACCAAGAAAACTCTCGCTCAGCCTGGCTGCCTTATCCCAAC CCCAACCCCAATTTTCAGTGCGGCACCATGGACCAGGGCCTGTACGTAAACCTGACGGAAAGAAACCTAC AGGATGCTCAGAAGTTCATTCTGATGCATGAAGTGGTGCAGCCAGTGACCACAGTGCCCTCTTTCATGGA GGACAACAGCCGCTTCTCCCACTTAGCTGTTGATGTTGTGCAAGGCAGGGAGACTCTGGTCCACATCATT TATCTGGCCACAGATTATGGCACCATTAAGAAAGTACGAGCTCCCCTGAGTCAGAGCTCAGGCAGCTGTT TGCTGGAAGAGATTGAGCTTTTCCCAGAGAGGAGGAGTGAGCCCATCAGGAGCCTGCAGATCCTCCACAG CCAGAGTGTCCTATTTGTGGGACTACAGGAGCATGTGGCCAAGATCCCCCTGAAGAGGTGCCACTTCCAT CAAACACGCAGTGCCTGCATTGGCGCTCAGGACCCTTACTGTGGCTGGGATGCGGTGATGAAGAAATGCA CCAGCCTGGAGGAGAGTCTGAGCATGACCCAGTGGGATCAGAGCATCCCCACCTGTCCGACCAGAAATCT CACTGTAGATGGGAGCTTTGGCCCATGGTCACCGTGGACACCCTGTACACACACTGATGGCACTGCTGTG GGCTCCTGCCTCTGCCGGTCCCGCTCCTGTGACAGCCCAGCTCCTCAGTGTGGTGGTTGGCAGTGTGAGG GCCCTAGAATGGAGATCACCAACTGTTCCAGGAATGGAGGCTGGACTCCCTGGACCTCCTGGTCTCCGTG CAGCACGACCTGTGGCATTGGCTTCCAAGTGCGGCAGCGATCCTGCAGCAACCCCACGCCCAGGCATGGC GGGCGCGTGTGCGTGGGCCAGAACAGGGAGGAAAGATACTGCAATGAACATTTGCTCTGCCCACCACACG TGTTCTGGACAGGCTGGGGACCTTGGGAACGGTGCACAGCCCAGTGCGGGGGTGGCATTCAAGCCCGCCG GAGGACCTGTGAAAATGGGCCTGACTGTGCAGGATGCAATGTGGAATACCAGCCTTGTAACACCAATGCA TGCCCGGAGCTGAAGAAGACCACACCCTGGACACCCTGGACCCCTGTCAACATCTCTGACAATGGAGGTC ACTATGAGCAGCGTTTCCGCTATACCTGTAAAGCTCGCCTGCCAGATCCAAATTTGCTGGAAGTAGGAAG ACAGAGGATAGAAATGCGGTACTGTTCCAGCGATGGAACCAGTGGCTGCTCCACAGACGGACTTTCTGGA GACTTTCTAAGAGCTGGGAGATACTCTGCTCATACAGTCAATGGGGCATGGTCAGCCTGGACTTCCTGGT CACAGTGCAGCAGAGACTGCAGCAGGGGCATTCGGAACCGGAAGCGTGTTTGCAACAACCCAGAACCCAA GTTTGGGGGCATGCCATGCCTTGGCCCATCGCTGGAGTTCCAGGAATGCAACATTTTACCTTGTCCAGTG GATGGTGTGTGGTCTTGCTGGTCATCCTGGTCTAAATGTTCAGCAACCTGTGGAGGTGGCCACTACATGA GAACCCGTTCTTGTTCGAATCCAGCCCCAGCATATGGAGGGGACATCTGCCTGGGACTGCACACAGAAGA GGCACTCTGCAACACACAGACCTGCCCAGAAAGCTGGTCAGAGTGGTCAGACTGGTCTGTGTGCGATGCA TCTGGTACCCAGGTCCGTGCTCGGCAGTGCATCCTTCTGTTTCCAGTGGGCAGCCAATGTTCTGGAAATA CTACAGAGAGCCGGCCTTGTGTATTTGACTCTAATTTCATCCCAGAAGTATCTGTGGCAAGATCCAGCAG CGTAGAAGAAAAAAGGTGTGGAGAGTTCAACATGTTCCACATGATGGCCGTGGGGCTTAGCAGTTCCATT CTTGGCTGCCTCCTCACACTGCTTGTCTACACCTACTGCCAGAGGTACCAGCAGCAGTCCCATGATGCAA CTGTCATCCACCCTGTCTCTCCTGCCGCCCTCAACAGCAGCATAACTAACCACATCAACAAACTGGACAA ATATGATTCTGTGGAGGCCATCAAGGCATTTAACAAAAACAACTTGATCCTAGAGGAGAGAAACAAATAC TTCAACCCACATCTCACTGGGAAGACCTATTCCAACGCCTACTTTACAGATCTCAACAATTATGATGAAT ACTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_009154 |
| Insert Size | 3225 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_009154.2, NP_033180.2 |
| RefSeq Size | 10809 bp |
| RefSeq ORF | 3225 bp |
| Locus ID | 20356 |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG223912 | Sema5a (tGFP-tagged) - Mouse sema domain seven thrombospondin repeats (type 1 and type 1-like) transmembrane domain (TM) and short cytoplasmic domain (semaphorin) 5A (Sema5a), (10ug) |
CNY 12,816.00 |
|
| MR223912 | Sema5a (Myc-DDK-tagged) - Mouse sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (Sema5a) |
CNY 11,216.00 |
|
| MR223912L3 | Lenti ORF clone of Sema5a (Myc-DDK-tagged) - Mouse sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (Sema5a) |
CNY 12,640.00 |
|
| MR223912L4 | Lenti ORF clone of Sema5a (mGFP-tagged) - Mouse sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (Sema5a) |
CNY 12,640.00 |
