Nefh (NM_010904) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	mKIAA0845; NF-H; NF200; Nfh
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC223532 representing NM_010904 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGAGCTTCGGCAGCGCCGATGCGCTGCTGGGCGCCCCGTTCGCGCCGCTGCACGGAGGCGGCAGCC TGCACTACTCGCTGAGCCGCAAGGCAGGCCCGGGCGGCACGCGCTCCGCGGCCGGCTCCTCCAGCGGCTT CCACTCGTGGGCGCGGACGTCCGTGAGCTCCGTGTCCGCCTCACCCAGCCGCTTCCGCGGCGCCGCCTCG AGCACCGACTCGCTAGACACCCTAAGCAACGGCCCAGAGGGCTGCGTGGTGGCGGCGGTGGCGGCGCGCA GCGAGAAGGAGCAGCTGCAGGCTCTGAACGACCGCTTCGCGGGCTACATCGACAAGGTGAGGCAGCTCGA GGCGCACAACCGCAGCCTGGAGGGCGAGGCGGCGGCGCTGCGGCAGCAGCAAGCCGGCCGCGCCGCCATG GGCGAGCTGTACGAGCGCGAGGTGCGCGAGATGCGCGGCGCCGTGCTGCGCCTCGGGGCGGCGCGCGGGC AGCTGCGCCTGGAGCAGGAGCACCTGCTGGAGGACATCGCTCACGTCCGCCAGCGGCTGGACGAGGAGGC CCGGCAGCGTGAGGAGGCGGAGGCGGCGGCGCGCGCCCTGGCGCGCTTCGCGCAGGAGGCGGAAGCGGCG CGCGTGGAGCTGCAGAAGAAGGCGCAGGCGCTGCAGGAGGAGTGCGGCTACCTGCGGCGCCACCACCAGG AGGAGGTGGGCGAGCTGCTCGGTCAGATCCAGGGCTGCGGGGCCGCGCAGGCGCAGGCTCAGGCCGAGGC TCGCGACGCCCTCAAGTGCGACGTGACGTCGGCGCTGCGGGAGATCCGCGCGCAGCTCGAAGGCCACGCG GTGCAGAGCACGCTGCAGTCCGAGGAGTGGTTCCGAGTGAGGTTGGACCGACTCTCAGAGGCAGCCAAAG TGAACACAGATGCTATGCGCTCGGCCCAAGAGGAGATAACTGAGTACCGGCGGCAGCTGCAAGCCAGGAC CACAGAGTTGGAGGCCCTGAAAAGCACCAAGGAGTCACTGGAGAGGCAGCGCTCTGAGCTAGAGGACCGT CATCAGGCAGACATTGCCTCCTACCAGGACGCTATTCAGCAGCTGGACAGTGAGCTGAGAAACACCAAGT GGGAGATGGCTGCACAGCTCCGAGAGTACCAGGACCTGCTCAACGTCAAGATGGCCCTGGACATTGAGAT TGCCGCTTACAGAAAGCTCCTGGAAGGCGAAGAGTGTCGGATTGGCTTTGGTCCGAGTCCCTTCTCTCTT ACTGAAGGACTCCCAAAAATTCCCTCCATATCCACGCACATAAAAGTCAAAAGCGAAGAGATGATAAAGG TAGTAGAGAAATCCGAGAAGGAAACTGTGATTGTAGAAGGACAGACAGAAGAGATCCGGGTGACGGAAGG AGTGACAGAAGAGGAGGACAAAGAGGCCCAAGGTCAGGAAGGAGAAGAAGCAGAAGAGGGAGAAGAAAAA GAAGAAGAGGAAGGAGCAGCAGCTACATCTCCCCCTGCAGAAGAGGCTGCATCTCCAGAAAAAGAAACCA AGTCTCGTGTGAAAGAAGAGGCCAAGTCCCCAGGTGAGGCCAAGTCCCCAGGTGAGGCCAAGTCCCCAGC TGAGGCCAAGTCCCCAGGTGAGGCCAAGTCCCCAGGTGAGGCCAAGTCCCCAGGTGAGGCCAAGTCTCCA GCTGAGCCCAAGTCTCCAGCTGAGCCCAAGTCTCCAGCTGAGGCCAAGTCACCAGCTGAGCCCAAGTCTC CAGCTACAGTGAAGTCTCCAGGTGAGGCCAAGTCACCATCTGAGGCCAAATCTCCAGCTGAAGCCAAATC TCCAGCTGAGGCCAAATCTCCAGCTGAGGCCAAATCTCCAGCTGAGGCCAAGTCACCAGCTGAAGCCAAG TCACCAGCTGAAGCCAAATCTCCAGCTACAGTGAAGTCTCCAGGTGAGGCCAAGTCACCATCTGAGGCCA AATCTCCAGCTGAAGCCAAATCTCCAGCTGAGGCCAAATCTCCAGCTGAGGCCAAATCTCCAGCTGAGGT CAAGTCACCAGGTGAGGCCAAGTCTCCAGCTGAGCCCAAGTCACCAGCTGAGGCCAAATCTCCAGCTGAG GTCAAGTCACCAGCTGAGGCCAAATCTCCAGCTGAGGTCAAGTCCCCAGGTGAGGCCAAGTCTCCAGCTG CAGTGAAGTCACCAGCTGAGGCCAAGTCTCCAGCTGCAGTCAAGTCCCCAGGTGAGGCCAAGTCCCCAGG TGAGGCCAAGTCACCAGCTGAGGCCAAATCTCCAGCTGAGGCCAAGTCACCAATTGAGGTAAAATCTCCA GAGAAGGCCAAGACCCCCGTCAAGGAAGGAGCAAAATCTCCAGCTGAGGCCAAGTCTCCTGAGAAGGCCA AGTCCCCCGTGAAGGAAGATATCAAGCCCCCAGCTGAGGCGAAATCCCCTGAGAAGGCCAAGAGCCCCGT GAAGGAAGGAGCAAAGCCTCCTGAGAAGGCCAAGCCTCTAGATGTGAAGTCTCCGGAAGCCCAGACTCCA GTACAGGAGGAAGCGAAGCACCCCACAGACATCAGACCCCCTGAGCAGGTGAAAAGTCCTGCCAAGGAGA AGGCCAAGTCCCCTGAGAAGGAAGAAGCCAAGACTTCTGAAAAGGTGGCTCCCAAGAAGGAAGAGGTGAA GTCCCCTGTGAAGGAGGAGGTAAAAGCCAAAGAACCCCCAAAGAAGGTAGAAGAAGAGAAGACACTGCCT ACACCAAAGACAGAGGCGAAGGAGAGTAAGAAAGACGAAGCTCCCAAGGAGGCCCCGAAGCCCAAGGTGG AGGAGAAGAAGGAAACTCCCACGGAAAAGCCCAAGGACTCTACAGCAGAAGCCAAGAAGGAAGAGGCTGG AGAGAAGAAGAAAGCCGTGGCCTCAGAGGAGGAGACTCCTGCCAAGTTGGGTGTGAAGGAAGAAGCTAAA CCCAAAGAGAAGACAGAGACAACCAAGACAGAAGCAGAAGACACCAAGGCCAAAGAACCTAGCAAACCCA CAGAGACGGAAAAGCCAAAGAAAGAGGAGATGCCAGCGGCACCAGAGAAGAAAGACACCAAGGAGGAGAA GACCACAGAGTCCAGGAAGCCTGAGGAGAAGCCCAAAATGGAGGCCAAGGTCAAGGAGGATGACAAGAGC CTTTCCAAAGAGCCTAGCAAACCCAAGACAGAAAAGGCTGAAAAATCCTCTAGCACAGACCAGAAAGAAA GCCAGCCCCCAGAGAAGACCACAGAGGACAAGGCCACCAAGGGAGAGAAGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_010904
Insert Size	3273 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_010904.3, NP_035034.2
RefSeq Size	4004 bp
RefSeq ORF	3273 bp
Locus ID	380684
UniProt ID	P19246
Gene Summary	Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.[UniProtKB/Swiss-Prot Function]

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