Nefh (NM_010904) Mouse Untagged Clone

CAT#: MC223532

Nefh (untagged) - Mouse neurofilament, heavy polypeptide (Nefh), (10ug)



  "NM_010904" in other vectors (2)

CNY 13,970.00


货期*
14周

规格
    • 10 ug

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms mKIAA0845; NF-H; NF200; Nfh
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC223532 representing NM_010904
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGAGCTTCGGCAGCGCCGATGCGCTGCTGGGCGCCCCGTTCGCGCCGCTGCACGGAGGCGGCAGCC
TGCACTACTCGCTGAGCCGCAAGGCAGGCCCGGGCGGCACGCGCTCCGCGGCCGGCTCCTCCAGCGGCTT
CCACTCGTGGGCGCGGACGTCCGTGAGCTCCGTGTCCGCCTCACCCAGCCGCTTCCGCGGCGCCGCCTCG
AGCACCGACTCGCTAGACACCCTAAGCAACGGCCCAGAGGGCTGCGTGGTGGCGGCGGTGGCGGCGCGCA
GCGAGAAGGAGCAGCTGCAGGCTCTGAACGACCGCTTCGCGGGCTACATCGACAAGGTGAGGCAGCTCGA
GGCGCACAACCGCAGCCTGGAGGGCGAGGCGGCGGCGCTGCGGCAGCAGCAAGCCGGCCGCGCCGCCATG
GGCGAGCTGTACGAGCGCGAGGTGCGCGAGATGCGCGGCGCCGTGCTGCGCCTCGGGGCGGCGCGCGGGC
AGCTGCGCCTGGAGCAGGAGCACCTGCTGGAGGACATCGCTCACGTCCGCCAGCGGCTGGACGAGGAGGC
CCGGCAGCGTGAGGAGGCGGAGGCGGCGGCGCGCGCCCTGGCGCGCTTCGCGCAGGAGGCGGAAGCGGCG
CGCGTGGAGCTGCAGAAGAAGGCGCAGGCGCTGCAGGAGGAGTGCGGCTACCTGCGGCGCCACCACCAGG
AGGAGGTGGGCGAGCTGCTCGGTCAGATCCAGGGCTGCGGGGCCGCGCAGGCGCAGGCTCAGGCCGAGGC
TCGCGACGCCCTCAAGTGCGACGTGACGTCGGCGCTGCGGGAGATCCGCGCGCAGCTCGAAGGCCACGCG
GTGCAGAGCACGCTGCAGTCCGAGGAGTGGTTCCGAGTGAGGTTGGACCGACTCTCAGAGGCAGCCAAAG
TGAACACAGATGCTATGCGCTCGGCCCAAGAGGAGATAACTGAGTACCGGCGGCAGCTGCAAGCCAGGAC
CACAGAGTTGGAGGCCCTGAAAAGCACCAAGGAGTCACTGGAGAGGCAGCGCTCTGAGCTAGAGGACCGT
CATCAGGCAGACATTGCCTCCTACCAGGACGCTATTCAGCAGCTGGACAGTGAGCTGAGAAACACCAAGT
GGGAGATGGCTGCACAGCTCCGAGAGTACCAGGACCTGCTCAACGTCAAGATGGCCCTGGACATTGAGAT
TGCCGCTTACAGAAAGCTCCTGGAAGGCGAAGAGTGTCGGATTGGCTTTGGTCCGAGTCCCTTCTCTCTT
ACTGAAGGACTCCCAAAAATTCCCTCCATATCCACGCACATAAAAGTCAAAAGCGAAGAGATGATAAAGG
TAGTAGAGAAATCCGAGAAGGAAACTGTGATTGTAGAAGGACAGACAGAAGAGATCCGGGTGACGGAAGG
AGTGACAGAAGAGGAGGACAAAGAGGCCCAAGGTCAGGAAGGAGAAGAAGCAGAAGAGGGAGAAGAAAAA
GAAGAAGAGGAAGGAGCAGCAGCTACATCTCCCCCTGCAGAAGAGGCTGCATCTCCAGAAAAAGAAACCA
AGTCTCGTGTGAAAGAAGAGGCCAAGTCCCCAGGTGAGGCCAAGTCCCCAGGTGAGGCCAAGTCCCCAGC
TGAGGCCAAGTCCCCAGGTGAGGCCAAGTCCCCAGGTGAGGCCAAGTCCCCAGGTGAGGCCAAGTCTCCA
GCTGAGCCCAAGTCTCCAGCTGAGCCCAAGTCTCCAGCTGAGGCCAAGTCACCAGCTGAGCCCAAGTCTC
CAGCTACAGTGAAGTCTCCAGGTGAGGCCAAGTCACCATCTGAGGCCAAATCTCCAGCTGAAGCCAAATC
TCCAGCTGAGGCCAAATCTCCAGCTGAGGCCAAATCTCCAGCTGAGGCCAAGTCACCAGCTGAAGCCAAG
TCACCAGCTGAAGCCAAATCTCCAGCTACAGTGAAGTCTCCAGGTGAGGCCAAGTCACCATCTGAGGCCA
AATCTCCAGCTGAAGCCAAATCTCCAGCTGAGGCCAAATCTCCAGCTGAGGCCAAATCTCCAGCTGAGGT
CAAGTCACCAGGTGAGGCCAAGTCTCCAGCTGAGCCCAAGTCACCAGCTGAGGCCAAATCTCCAGCTGAG
GTCAAGTCACCAGCTGAGGCCAAATCTCCAGCTGAGGTCAAGTCCCCAGGTGAGGCCAAGTCTCCAGCTG
CAGTGAAGTCACCAGCTGAGGCCAAGTCTCCAGCTGCAGTCAAGTCCCCAGGTGAGGCCAAGTCCCCAGG
TGAGGCCAAGTCACCAGCTGAGGCCAAATCTCCAGCTGAGGCCAAGTCACCAATTGAGGTAAAATCTCCA
GAGAAGGCCAAGACCCCCGTCAAGGAAGGAGCAAAATCTCCAGCTGAGGCCAAGTCTCCTGAGAAGGCCA
AGTCCCCCGTGAAGGAAGATATCAAGCCCCCAGCTGAGGCGAAATCCCCTGAGAAGGCCAAGAGCCCCGT
GAAGGAAGGAGCAAAGCCTCCTGAGAAGGCCAAGCCTCTAGATGTGAAGTCTCCGGAAGCCCAGACTCCA
GTACAGGAGGAAGCGAAGCACCCCACAGACATCAGACCCCCTGAGCAGGTGAAAAGTCCTGCCAAGGAGA
AGGCCAAGTCCCCTGAGAAGGAAGAAGCCAAGACTTCTGAAAAGGTGGCTCCCAAGAAGGAAGAGGTGAA
GTCCCCTGTGAAGGAGGAGGTAAAAGCCAAAGAACCCCCAAAGAAGGTAGAAGAAGAGAAGACACTGCCT
ACACCAAAGACAGAGGCGAAGGAGAGTAAGAAAGACGAAGCTCCCAAGGAGGCCCCGAAGCCCAAGGTGG
AGGAGAAGAAGGAAACTCCCACGGAAAAGCCCAAGGACTCTACAGCAGAAGCCAAGAAGGAAGAGGCTGG
AGAGAAGAAGAAAGCCGTGGCCTCAGAGGAGGAGACTCCTGCCAAGTTGGGTGTGAAGGAAGAAGCTAAA
CCCAAAGAGAAGACAGAGACAACCAAGACAGAAGCAGAAGACACCAAGGCCAAAGAACCTAGCAAACCCA
CAGAGACGGAAAAGCCAAAGAAAGAGGAGATGCCAGCGGCACCAGAGAAGAAAGACACCAAGGAGGAGAA
GACCACAGAGTCCAGGAAGCCTGAGGAGAAGCCCAAAATGGAGGCCAAGGTCAAGGAGGATGACAAGAGC
CTTTCCAAAGAGCCTAGCAAACCCAAGACAGAAAAGGCTGAAAAATCCTCTAGCACAGACCAGAAAGAAA
GCCAGCCCCCAGAGAAGACCACAGAGGACAAGGCCACCAAGGGAGAGAAGTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_010904
Insert Size 3273 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_010904.3, NP_035034.2
RefSeq Size 4004 bp
RefSeq ORF 3273 bp
Locus ID 380684
UniProt ID P19246
Gene Summary Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...