Msh3 (NM_010829) Mouse Untagged Clone

CAT#: MC223548

Msh3 (untagged) - Mouse mutS homolog 3 (E. coli) (Msh3), (10ug)



  "NM_010829" in other vectors (4)

CNY 14,060.00


货期*
7周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms D13Em1; Rep-3; Rep3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC223548 representing NM_010829
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCCCGCGGGAAATCCGCGTCGGGAGGCTCTACTGCCGCCGGCCCAGGCCCGGGGAGGCAAACGGTTC
TAAGCCGGTTCTTCAGGTCTGCGGGAAGCCTAAGATCCAGCGCGTCCTCCACGGAGCCAGCAGAGAAGGT
GACAGAAGGTGACAGCAGGAAGAGGTCACTGGGAAATGGTGGGCCCACTAAGAAAAAAGCAAGGAAAGTT
CCAGAGAAGGAGGAAGAAAACATCTCAGTAGCAGCTCACCACCCTGAGGCAAAGAAATGTCTGAGGCCCA
GGATTGTTTTAAAGTCCCTGGAAAAGTTGAAAGAATTCTGCTGTGATTCTGCCCTCCCTCAAAACAGAGT
CCAGACAGAAGCTCTTCGGGAGAGACTTGAAGTTCTGCCAAGGTGTACTGATTTTGAAGATATCACTCTG
CAACGTGCAAAGAATGCGGTTTTGTCCGAAGATTCCAAATCTCAGGCTAATCAGAAAGACAGTCAATTTG
GACCCTGCCCTGAAGTTTTCCAGAAGACTTCCGATTGTAAACCTTTTAACAAGCGATCCAAGAGCGTCTA
TACACCGTTAGAACTGCAGTACTTAGATATGAAGCAGCAGCATAAAGACGCAGTTTTGTGTGTGGAGTGT
GGTTACAAGTACAGATTCTTTGGGGAAGATGCAGAAATTGCTGCCCGGGAACTCAATATTTATTGCCACT
TAGACCACAACTTTATGACTGCAAGCATACCTACCCACAGACTGTTCGTGCATGTCCGCCGACTTGTGGC
CAAAGGGTACAAGGTGGGAGTTGTGAAGCAAACTGAAACTGCCGCATTAAAGGCCATCGGAGACAATAAA
AGTTCTGTCTTCTCCCGGAAATTGACCGCTCTTTATACGAAATCCACACTTATTGGTGAAGATGTGAATC
CTCTCATCAGGCTGGATGATTCTGTAAATATCGATGAGGTAATGACAGATACATCTACCAACTATCTTCT
GTGTATCTACGAAGAAAAGGAGAACATTAAAGACAAAAAGAAGGGGAACCTTTCCGTTGGTATCGTGGGA
GTGCAGCCCGCAACTGGCGAGGTTGTGTTTGACTGCTTCCAGGACTCTGCTTCCAGACTGGAGCTGGAGA
CTCGCATATCAAGCCTGCAGCCTGTGGAATTACTGCTTCCCTCCGACCTGTCTGTGCCAACAGAGATGCT
CATCCAAAGAGCCACCAATGTCAGTGTGCGGGATGACAGGATTCGAGTAGAAAGGATGAATAATACTTAC
TTTGAATACAGCCATGCTTTCCAGACAGTTACGGAGTTTTATGCTAGAGAGATAGTTGACAGCCAGGGCT
CTCAGAGTCTCTCTGGTGTCATTAACTTGGAGAAGCCTGTGATCTGCGCCTTGGCTGCCGTAATAAGGTA
CCTCAAGGAATTTAACCTGGAAAAGATGCTGTCCAAACCCGAGAGTTTTAAACAGCTGTCAAGTGGAATG
GAATTCATGAGAATTAATGGGACCACGCTAAGGAATCTGGAGATCCTTCAGAATCAGACTGATATGAAGA
CCAAAGGAAGCTTGCTTTGGGTTTTAGACCACACTAAAACTTCATTTGGGAGAAGGAAGTTAAAGAATTG
GGTTACCCAGCCACTGCTTAAATTAAGGGAAATAAATGCCCGACTTGATGCAGTATCTGATGTTCTCCAC
TCAGAATCCAGTGTGTTTGAGCAGATAGAAAACCTTCTTCGTAAGTTACCGGATGTCGAGAGAGGACTAT
GTAGCATTTATCACAAGAAGTGCTCTACCCAAGAGTTCTTCTTGATCGTCAAAAGCCTATGTCAACTGAA
GTCAGAGCTTCAAGCATTAATGCCTGCTGTTAATTCCCACGTTCAGTCCGACTTACTCCGAGCACTCATC
GTGGAGGCTCCTGAGCTCCTCAGCCCAGTGGAACATTACTTAAAGGTTCTGAATGGGCCAGCTGCCAAAG
TTGGCGATAAAACTGAATTATTCAAAGACCTCTCTGACTTCCCTTTAATAAAAAAGAGGAAAAATGAAAT
TCAGGAAGTCATTCACAGCATCCAAATGCGTTTACAGGAATTTCGGAAAATATTAAAACTTCCTTCTTTA
CAGTATGTGACTGTATCAGGACAAGAGTTTATGATTGAAATAAAGAACTCGGCTGTATCTTGCATCCCAG
CTGATTGGGTGAAGGTTGGAAGCACAAAAGCTGTGAGCCGCTTTCACCCTCCCTTCATCGTGGAAAGCTA
CAGACGTCTGAATCAGCTCCGGGAGCAGCTAGTCCTTGACTGCAATGCTGAATGGCTTGGCTTCCTAGAG
AATTTTGGTGAACATTATCACACTTTGTGTAAGGCTGTGGATCACCTAGCAACTGTTGACTGTATTTTCT
CCCTGGCCAAGGTCGCTAAACAAGGAAATTACTGCAGGCCAACTCTACAAGAAGAAAAGAAGATCATCAT
AAAAAATGGAAGGCACCCTATGATTGATGTGTTGCTGGGTGAACAAGATCAGTTTGTGCCCAACAGTACG
AGTTTATCACAGGACTCGGAGAGAGTAATGATAATCACCGGACCAAACATGGGTGGGAAAAGCTCCTACA
TAAAACAGGTTGCACTGGTTACCATCATGGCTCAGATTGGCTCCTACGTCCCTGCAGAGGAAGCCACAAT
TGGGATTGTGGATGGCATTTTCACAAGGATGGGTGCTGCAGACAATATATACAAAGGCCGGAGTACTTTC
ATGGAAGAACTGACGGACACAGCGGAAATAATCAGGAGAGCGTCCCCACAGTCCTTGGTTATCCTGGATG
AGCTGGGGAGAGGGACAAGCACCCATGACGGAATCGCCATCGCCTATGCAACTCTAGAGTATTTTATCAG
AGACGTGAAATCCTTAACACTGTTCGTTACCCACTATCCACCAGTCTGTGAACTAGAAAAATGTTACCCA
GAACAAGTGGGGAATTACCACATGGGATTCTTGGTCAATGAAGATGAAAGCAAGCAGGACTCAGGTGACA
TGGAGCAGATGCCGGATTCTGTCACTTTTCTCTATCAGATAACCCGAGGAATTGCAGCGAGAAGTTATGG
ACTTAATGTGGCGAAGTTAGCAGATGTACCTAGAGAAGTCTTACAGAAAGCCGCGCACAAGTCTAAGGAG
CTGGAAGGCTTAGTCAGCCTGAGAAGGAAAAGGCTTGAATGTTTTACCGACTTATGGACGACACACAGTG
TGAAGGACCTGCACACATGGGCAGATAAGCTGGAAATGGAGGAAATACAGACTTCTCTCCCACATTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_010829
Insert Size 3288 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_010829.2, NP_034959.2
RefSeq Size 3945 bp
RefSeq ORF 3288 bp
Locus ID 17686
UniProt ID P13705
Gene Summary Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. MutS beta recognizes large insertion-deletion loops (IDL) up to 13 nucleotides long. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis (By similarity).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (1) encodes the longer isoform (1).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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