Trpm8 (NM_134252) Mouse Untagged Clone
CAT#: MC223594
Trpm8 (untagged) - Mouse transient receptor potential cation channel, subfamily M, member 8 (Trpm8), (10ug)
CNY 14,160.00
Product images
![](https://cdn.origene.com/img/defaults-img.jpg)
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | CMR1; LTrpC-6; LTRPC6; Trp-p8; TRPP8 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC223594 representing NM_134252
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCTTCGAGGGAGCCAGGCTCAGCATGAGGAGCCGCAGAAATGGTACTATGGGCAGCACCCGGACCC TGTACTCCAGTGTATCTCGGAGCACAGACGTGTCCTACAGTGACAGTGATTTGGTGAATTTTATTCAGGC AAATTTTAAAAAACGAGAATGTGTCTTCTTTACCAGAGACTCCAAGGCCATGGAGAACATATGCAAGTGT GGTTATGCCCAGAGCCAGCACATCGAAGGCACCCAGATCAACCAAAATGAGAAGTGGAACTACAAAAAAC ATACCAAGGAGTTTCCAACAGACGCCTTCGGGGACATTCAGTTTGAGACTCTGGGGAAGAAAGGCAAGTA CTTACGCTTGTCCTGTGACACCGACTCTGAAACTCTCTACGAACTGCTGACCCAGCACTGGCACCTCAAA ACACCCAACCTGGTCATTTCAGTGACGGGTGGAGCCAAAAACTTTGCTTTGAAGCCACGCATGCGCAAGA TCTTCAGCAGGCTGATTTACATCGCACAGTCTAAAGGTGCGTGGATTCTCACTGGAGGCACTCACTACGG CCTGATGAAGTACATAGGCGAGGTGGTGAGAGACAACACCATCAGCAGGAACTCAGAAGAGAACATCGTG GCCATTGGCATCGCAGCATGGGGCATGGTCTCCAACAGGGACACCCTCATCAGGAGCTGTGATGATGAGG GACATTTTTCAGCTCAATACATCATGGATGACTTTACCAGAGACCCTCTATACATCCTGGACAACAACCA TACCCACCTGCTGCTTGTGGACAACGGTTGTCATGGACACCCCACAGTGGAAGCCAAGCTCCGGAATCAG CTGGAAAAGTACATCTCTGAGCGCACCAGTCAAGATTCCAACTATGGTGGTAAGATCCCCATCGTGTGTT TTGCCCAAGGAGGTGGAAGAGAGACTCTAAAAGCCATCAACACCTCTGTCAAAAGCAAGATCCCTTGTGT GGTGGTGGAAGGCTCGGGGCAGATTGCTGATGTGATCGCCAGCCTGGTGGAGGTGGAGGATGTTTTAACC TCTTCCATGGTCAAAGAGAAGCTGGTACGCTTTTTACCACGCACTGTGTCCCGGCTGCCTGAAGAGGAAA TTGAGAGCTGGATCAAATGGCTCAAAGAAATTCTTGAGAGTTCTCACCTACTCACAGTAATTAAGATGGA AGAGGCTGGAGATGAGATTGTGAGCAACGCCATTTCCTATGCGCTGTACAAAGCCTTCAGCACTAATGAG CAAGACAAGGACAACTGGAATGGACAGCTGAAGCTTCTGCTGGAGTGGAACCAGTTGGACCTTGCCAGTG ATGAGATCTTCACCAATGATCGCCGCTGGGAGTCTGCCGACCTTCAGGAGGTCATGTTCACGGCTCTCAT AAAGGACAGACCCAAGTTTGTCCGCCTCTTTCTGGAGAATGGCCTGAATCTGCAGAAGTTTCTCACCAAT GAAGTCCTCACAGAGCTCTTCTCCACCCACTTCAGCACCCTAGTGTACCGGAATCTGCAGATCGCCAAGA ACTCCTACAATGACGCACTCCTCACCTTTGTCTGGAAGTTGGTGGCAAACTTCCGTCGAAGCTTCTGGAA AGAGGACAGAAGCAGCAGGGAGGACTTGGATGTGGAACTCCATGATGCATCTCTCACCACCCGGCACCCG CTGCAAGCTCTCTTCATCTGGGCCATTCTTCAGAACAAGAAGGAACTCTCCAAGGTCATTTGGGAGCAGA CCAAAGGCTGTACTCTGGCAGCCTTGGGGGCCAGCAAGCTTCTGAAGACCCTGGCCAAAGTTAAGAATGA TATCAACGCTGCTGGGGAATCGGAGGAACTGGCCAATGAATATGAGACCCGAGCAGTGGAGTTGTTCACC GAGTGTTACAGCAATGATGAAGACTTGGCAGAACAGCTACTGGTCTACTCCTGCGAAGCCTGGGGTGGGA GCAACTGTCTGGAGCTGGCAGTGGAGGCTACAGATCAGCATTTCATCGCTCAGCCTGGGGTCCAGAATTT CCTTTCTAAGCAATGGTATGGAGAGATTTCCCGAGACACGAAGAACTGGAAGATTATCCTGTGTCTATTC ATCATCCCCTTAGTGGGCTGTGGCCTCGTATCATTTAGGAAGAAACCCATTGACAAGCACAAGAAGCTGC TGTGGTACTATGTGGCCTTCTTCACGTCGCCCTTCGTGGTCTTCTCCTGGAACGTGGTCTTCTACATCGC CTTCCTCCTGCTGTTTGCCTATGTGCTGCTCATGGACTTCCACTCAGTGCCACACACCCCCGAGCTGATC CTCTACGCCCTGGTCTTCGTCCTCTTCTGTGATGAAGTGAGGCAGTGGTACATGAACGGAGTGAATTATT TCACCGACCTATGGAACGTTATGGACACCCTGGGACTCTTCTACTTCATAGCGGGTATTGTATTCCGGCT CCACTCTTCTAATAAAAGCTCGTTGTACTCTGGGCGCGTCATTTTCTGTCTGGATTACATTATATTCACG CTAAGGCTCATCCACATTTTCACCGTCAGCAGGAACTTGGGACCCAAGATTATAATGCTGCAGCGGATGC TGATCGACGTTTTCTTCTTCCTGTTCCTCTTTGCTGTGTGGATGGTGGCCTTTGGCGTGGCCAGACAGGG GATCCTAAGGCAAAATGAACAGCGCTGGAGATGGATCTTCCGCTCTGTCATCTATGAGCCCTACCTGGCC ATGTTTGGCCAGGTTCCCAGTGACGTGGATAGTACCACATATGACTTCTCCCACTGTACCTTCTCGGGAA ATGAGTCCAAGCCACTGTGTGTGGAGCTGGATGAGCACAACCTGCCCCGCTTCCCTGAGTGGATCACCAT TCCGCTGGTGTGCATCTACATGCTCTCCACCAATATCCTTCTGGTCAACCTCCTGGTCGCCATGTTTGGC TACACGGTAGGCATTGTACAGGAGAACAACGACCAGGTCTGGAAATTCCAGCGGTACTTCCTGGTGCAGG AGTACTGCAACCGCCTAAACATCCCCTTCCCCTTCGTTGTCTTCGCTTATTTCTACATGGTGGTGAAGAA GTGTTTCAAATGCTGCTGTAAAGAGAAGAATATGGAGTCTAATGCCTGCTGTTTCAGAAATGAGGACAAT GAGACTTTGGCGTGGGAGGGTGTCATGAAGGAGAATTACCTTGTCAAGATCAACACGAAAGCCAACGACA ACTCAGAGGAGATGAGGCATCGGTTTAGACAACTGGACTCAAAGCTTAACGACCTCAAAAGTCTTCTGAA AGAGATTGCTAATAACATCAAGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_134252 |
Insert Size | 3315 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_134252.3, NP_599013.1 |
RefSeq Size | 3869 bp |
RefSeq ORF | 3315 bp |
Locus ID | 171382 |
UniProt ID | Q8R4D5 |
Gene Summary | Receptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium. Temperature sensing is tightly linked to voltage-dependent gating. Activated upon depolarization, changes in temperature resulting in graded shifts of its voltage-dependent activation curves. The chemical agonists menthol functions as a gating modifier, shifting activation curves towards physiological membrane potentials. Temperature sensitivity arises from a tenfold difference in the activation energies associated with voltage-dependent opening and closing.[UniProtKB/Swiss-Prot Function] |
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG225279 | Trpm8 (tGFP-tagged) - Mouse transient receptor potential cation channel subfamily M member 8 (Trpm8), (10ug) |
CNY 9,220.00 |
|
MR225279 | Trpm8 (Myc-DDK-tagged) - Mouse transient receptor potential cation channel, subfamily M, member 8 (Trpm8) |
CNY 7,696.00 |
|
MR225279L3 | Lenti ORF clone of Trpm8 (Myc-DDK-tagged) - Mouse transient receptor potential cation channel, subfamily M, member 8 (Trpm8) |
CNY 10,260.00 |
|
MR225279L4 | Lenti ORF clone of Trpm8 (mGFP-tagged) - Mouse transient receptor potential cation channel, subfamily M, member 8 (Trpm8) |
CNY 10,260.00 |