Trpa1 (NM_177781) Mouse Untagged Clone

CAT#: MC223667

Trpa1 (untagged) - Mouse transient receptor potential cation channel, subfamily A, member 1 (Trpa1), (10ug)



  "NM_177781" in other vectors (6)

CNY 12,544.00


货期*
现货

规格
    • 10 ug

Cited in 1 publication.

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms Anktm1; TRPA1b
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC223667 representing NM_177781
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGCGCGGCTTGAGGAGGATTCTGCTCCCGGAGGAAAGGAAGGAGGTCCAGGGCGTTGTCTATCGCG
GCGTCGGGGAAGACATGGACTGCTCCAAGGAATCCTTTAAGGTGGACATTGAAGGAGATATGTGTAGATT
AGAAGACTTCATCAAGAACCGAAGAAAACTAAGCAAATATGAGGATGAAAATCTCTGTCCTCTGCATCAC
GCAGCAGCAGAAGGTCAAGTTGAACTGATGGAACTGATCATCAATGGTTCTTCGTGTGAAGTGCTGAATA
TAATGGATGGTTATGGAAATACCCCACTGCATTGTGCTGCAGAAAAAAATCAAGTTGAAAGTGTAAAGTT
TCTTCTCAGCCAAGGAGCAAATCCAAACCTCCGAAATAGAAACATGATGTCACCCCTTCACATAGCTGTG
CATGGCATGTACAACGAAGTGATCAAGGTGTTGACTGAGCACAAGGCCACTAACATCAATTTAGAAGGAG
AGAATGGGAACACGGCTTTGATGTCCACGTGTGCCAAAGACAACAGTGAAGCTTTGCAAATTTTGTTAGA
AAAAGGAGCTAAGCTGTGTAAATCAAATAAGTGGGGAGACTACCCTGTGCACCAGGCAGCATTTTCAGGT
GCCAAAAAATGCATGGAATTAATCTTAGCATATGGTGAAAAGAACGGCTACAGCAGGGAGACTCACATTA
ATTTTGTGAATCACAAGAAAGCCAGCCCTCTCCACCTAGCAGTTCAAAGCGGAGACTTGGACATGATTAA
GATGTGCCTGGACAACGGTGCACACATCGACATGATGGAGAATGCCAAATGCATGGCCCTCCATTTTGCT
GCAACCCAGGGAGCCACTGACATCGTTAAGCTCATGATCTCATCCTATACCGGAAGTAGTGATATTGTGA
ATGCAGTTGATGGCAATCAGGAGACCCTGCTTCACAGAGCCTCGTTATTTGATCACCATGACCTGGCAGA
ATACCTAATATCAGTGGGAGCAGACATCAACAGCACTGATTCTGAAGGACGCTCTCCACTTATTTTAGCA
ACAGCTTCTGCATCCTGGAACATTGTGAATTTGCTCCTCTGTAAAGGTGCCAAAGTAGACATAAAAGATC
ATCTTGGACGTAACTTTTTGCATTTGACTGTGCAGCAGCCTTATGGACTAAGAAATTTGCGGCCTGAGTT
TATGCAGATGCAACACATCAAAGAGCTGGTGATGGATGAAGACAATGACGGATGCACACCTCTCCATTAT
GCCTGTAGGCAGGGGGTTCCTGTCTCTGTAAATAACCTCCTTGGCTTCAATGTGTCCATTCATAGCAAAA
GTAAAGATAAGAAGTCGCCCCTGCATTTTGCAGCCAGTTATGGGCGCATCAATACATGTCAGAGACTTCT
GCAAGACATAAGTGATACGAGGCTTTTGAATGAAGGGGATCTCCATGGGATGACCCCTCTCCACCTGGCA
GCAAAAAATGGGCATGATAAAGTCGTTCAACTCCTTCTGAAGAAAGGGGCCTTATTTCTCAGTGACCACA
ATGGCTGGACTGCTTTGCATCACGCCTCCATGGGTGGGTACACTCAGACCATGAAGGTCATTCTTGATAC
TAACTTGAAATGCACAGACCGACTAGATGAAGAAGGGAACACAGCACTCCACTTTGCAGCACGGGAAGGC
CATGCCAAGGCTGTTGCAATGCTTTTGAGCTACAATGCTGACATCCTCCTGAACAAGAAGCAAGCTTCCT
TTCTGCATATTGCCCTGCACAATAAGCGCAAGGAAGTGGTTCTCACAACCATCAGAAATAAAAGATGGGA
TGAGTGTCTTCAAGTTTTCACTCATAATTCTCCAAGCAATCGATGTCCAATCATGGAGATGGTAGAATAC
CTCCCCGAGTGCATGAAAGTTCTTTTAGATTTCTGCATGATACCTTCCACAGAAGACAAGTCCTGTCAAG
ACTACCATATTGAGTATAATTTCAAGTATCTCCAATGCCCATTATCCATGACCAAAAAAGTAGCACCTAC
CCAGGATGTGGTATATGAGCCTCTTACAATCCTCAATGTCATGGTCCAACATAACCGCATAGAACTCCTC
AACCACCCTGTGTGTAGGGAGTACTTACTCATGAAATGGTGTGCCTATGGATTCAGAGCCCATATGATGA
ACCTAGGATCTTATTGTCTTGGTCTCATACCCATGACCCTTCTTGTTGTCAAAATACAGCCTGGAATGGC
CTTCAATTCTACTGGAATAATCAATGGAACTAGTAGTACTCATGAGGAAAGAATAGACACTCTGAATTCA
TTTCCAATAAAAATATGTATGATTCTAGTTTTTTTATCAAGTATATTTGGATATTGCAAAGAAGTGATCC
AAATTTTCCAACAGAAAAGGAATTACTTCCTGGATTACAACAATGCTCTGGAATGGGTTATCTATACAAC
TAGTATCATCTTCGTGTTGCCCTTGTTCCTCAACATCCCAGCGTATATGCAGTGGCAATGTGGAGCAATA
GCGATATTCTTCTACTGGATGAACTTCCTACTGTATCTTCAAAGGTTTGAGAACTGTGGAATTTTCATTG
TTATGTTGGAGGTGATTTTTAAAACATTGCTGAGATCGACCGGAGTGTTTATCTTCCTCCTACTGGCTTT
TGGCCTCAGCTTTTATGTTCTCCTGAATTTCCAAGATGCCTTCAGCACCCCATTGCTTTCCTTAATCCAG
ACATTCAGTATGATGCTAGGAGACATCAATTATCGAGATGCCTTCCTAGAACCATTGTTTAGAAATGAGT
TGGCATACCCAGTCCTGACCTTTGGGCAGCTTATTGCCTTCACAATGTTTGTCCCAATTGTTCTCATGAA
CTTACTGATTGGCTTGGCGGTTGGGGACATTGCTGAGGTCCAGAAGCATGCGTCATTGAAGAGGATTGCT
ATGCAGGTGGAACTTCATACCAACTTAGAAAAAAAGCTGCCACTCTGGTACTTACGCAAAGTGGATCAGA
GGTCCACCATCGTGTATCCAAATAGACCCAGGCACGGCAGGATGCTACGGTTTTTTCATTACTTTCTTAA
TATGCAAGAAACACGACAAGAAGTACCAAACATTGACACATGCTTGGAAATGGAAATATTGAAACAGAAA
TATCGGCTGAAGGACCTCACTTCCCTCTTGGAAAAGCAGCATGAGCTCATCAAACTCATCATCCAGAAGA
TGGAGATCATCTCAGAGACAGAAGATGAAGATAACCATTGCTCTTTCCAAGACAGGTTCAAGAAGGAGAG
GCTGGAACAGATGCACAGCAAGTGGAATTTTGTCTTAAACGCAGTTAAGACTAAAACACATTGTTCTATT
AGCCACCCGGACTTTTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_177781
Insert Size 3378 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_177781.4, NP_808449.1
RefSeq Size 4263 bp
RefSeq ORF 3378 bp
Locus ID 277328
UniProt ID Q8BLA8
Gene Summary Receptor-activated non-selective cation channel involved in detection of pain and possibly also in cold perception and inner ear function. Has a central role in the pain response to endogenous inflammatory mediators and to a diverse array of volatile irritants, such as mustard oil, cinnamaldehyde, garlic and acrolein, an irritant from tears gas and vehicule exhaust fumes. Acts also as an ionotropic cannabinoid receptor by being activated by delta(9)-tetrahydrocannabinol (THC), the psychoactive component of marijuana. Is also activated by menthol (in vitro) (By similarity). May be a component for the mechanosensitive transduction channel of hair cells in inner ear, thereby participating in the perception of sounds. Probably operated by a phosphatidylinositol second messenger system.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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