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Ddb1 (NM_015735) Mouse Untagged Clone
CAT＃: MC223704
Ddb1 (untagged) - Mouse damage specific DNA binding protein 1 (Ddb1), (10ug)
CNY 14,730.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 127kDa; AA408517; p127-Ddb1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC223704 representing NM_015735
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGTACAACTACGTCGTAACGGCGCAGAAGCCTACTGCAGTGAACGGCTGTGTGACCGGACACTTTA CTTCAGCGGAAGATCTGAACCTGTTGATTGCTAAAAACACAAGATTAGAGATCTATGTGGTCACTGCGGA GGGTCTTCGGCCAGTCAAAGAGGTGGGAATGTACGGGAAGATTGCAGTCATGGAGCTCTTCAGGCCCAAG GGGGAGAGCAAAGACCTGCTGTTCATCTTGACTGCTAAATACAATGCCTGTATCTTGGAGTATAAGCAGA GTGGCGAAAGCATTGACATCATTACAAGAGCCCATGGCAATGTCCAGGACCGCATTGGTCGCCCCTCGGA GACTGGCATCATTGGCATCATTGACCCTGAGTGCCGTATGATTGGCCTTCGACTCTACGATGGCCTTTTC AAGGTCATTCCACTAGACCGAGACAATAAAGAGCTCAAGGCCTTTAACATCCGCCTAGAGGAACTGCACG TCATTGATGTCAAGTTCCTGTATGGTTGTCAAGCACCTACCATTTGCTTTGTCTACCAGGATCCTCAGGG GCGGCATGTGAAAACCTATGAGGTGTCTCTTCGAGAGAAGGAGTTCAACAAGGGCCCTTGGAAACAGGAG AATGTAGAAGCCGAAGCTTCAATGGTGATAGCAGTCCCAGAGCCTTTTGGAGGTGCCATCATCATTGGAC AGGAATCAATCACCTATCATAACGGTGATAAATACCTGGCAATTGCTCCTCCTATCATTAAGCAAAGCAC TATTGTGTGCCATAACCGGGTGGATCCTAATGGCTCGCGGTACCTTCTGGGAGATATGGAGGGACGGCTT TTCATGCTGCTCTTAGAGAAGGAGGAGCAGATGGATGGCACTGTCACCCTCAAGGACCTTCGAGTGGAAC TCCTCGGAGAGACCTCCATTGCTGAGTGCCTAACTTATCTTGATAATGGTGTTGTATTTGTCGGGTCTCG CCTAGGTGACTCCCAGCTCGTAAAGCTCAATGTCGACAGCAACGAACAAGGCTCCTATGTAGTGGCCATG GAAACCTTTACCAATTTAGGACCCATTGTGGATATGTGTGTGGTGGACCTGGAGAGGCAGGGACAGGGTC AGCTGGTCACTTGCTCTGGAGCTTTCAAGGAAGGTTCCTTGCGGATCATCCGGAACGGCATTGGAATCCA TGAGCATGCCAGCATTGACTTACCAGGCATCAAAGGTTTATGGCCTCTGCGCTCTGACCCCGGTCGGGAG ACGGATGACACTTTGGTGCTCTCTTTTGTGGGCCAGACAAGAGTTCTCATGCTAAACGGAGAGGAAGTGG AAGAGACAGAACTGATGGGCTTTGTGGACGATCAGCAGACTTTCTTTTGTGGCAACGTGGCTCATCAGCA ACTTATCCAGATCACCTCAGCATCTGTGAGGTTGGTGTCTCAAGAGCCCAAAGCTTTGGTCAGCGAGTGG AAAGAGCCTCAGGGCAAGAACATCAGTGTGGCCTCCTGTAACAGCAGCCAGGTGGTTGTTGCCGTGGGAA GGGCACTATATTACCTTCAGATCCACCCTCAGGAGCTCCGGCAAATCAGCCACACAGAGATGGAACATGA AGTGGCATGCTTGGATATCACCCCATTAGGGGACAGCAATGGCCTCTCCCCACTTTGTGCCATTGGACTT TGGACAGACATCTCAGCTCGTATCCTGAAGCTCCCATCCTTTGAACTACTACACAAGGAGATGCTGGGTG GAGAGATCATTCCTCGATCCATCCTGATGACCACTTTTGAGAGTAGCCACTACCTCCTTTGTGCCTTGGG AGATGGGGCTCTTTTCTACTTTGGGCTCAACATCGAGACAGGTTTACTGAGTGACCGTAAAAAGGTGACT TTGGGCACCCAGCCCACGGTGTTGAGGACATTCCGTTCTCTTTCTACCACCAACGTCTTTGCTTGCTCTG ACCGGCCCACTGTCATCTATAGCAGCAATCACAAGTTGGTCTTCTCCAATGTCAACCTCAAGGAGGTGAA CTATATGTGTCCTCTCAACTCAGATGGCTATCCTGACAGTCTGGCATTGGCCAATAACAGCACTCTCACC ATTGGCACCATCGATGAGATCCAGAAGCTCCATATTCGCACAGTTCCCCTCTATGAGTCTCCCAGGAAGA TCTGCTATCAGGAAGTGTCTCAGTGCTTTGGGGTCCTTTCCAGCCGCATTGAAGTCCAAGATAGCAGTGG AGGCACTACTGCTTTGAGGCCCAGTGCCAGCACCCAGGCCCTGTCCAGCAGCGTCAGCTCCAGCAAACTT TTCTCCAGCAGTACGGCTCCTCACGAGACCTCCTTTGGAGAAGAAGTGGAGGTGCACAACCTCCTCATCA TTGACCAGCACACCTTTGAAGTGCTTCACGCCCATCAGTTTCTACAAAACGAGTATGCCCTCAGCCTGGT CTCCTGCAAGTTGGGTAAAGACCCCAACACTTACTTCATTGTGGGCACAGCCATGGTATATCCTGAAGAG GCAGAGCCCAAGCAGGGTCGTATTGTGGTCTTCCAGTATTCAGATGGAAAACTACAGACTGTGGCTGAAA AGGAAGTAAAAGGAGCTGTGTACTCTATGGTGGAGTTTAATGGGAAGCTGCTTGCCAGCATCAATAGCAC GGTGCGGCTCTATGAGTGGACCACAGAAAAGGAGCTTCGCACTGAGTGTAATCACTACAACAACATCATG GCCCTGTACCTGAAGACCAAGGGCGACTTCATCCTGGTGGGCGACCTCATGCGCTCAGTGCTGCTGCTTG CCTACAAGCCTATGGAAGGAAACTTTGAAGAGATTGCCCGAGACTTTAATCCCAACTGGATGAGTGCAGT GGAGATCTTGGATGATGACAATTTTCTGGGAGCTGAAAATGCCTTTAACTTGTTTGTGTGTCAGAAGGAC AGTGCTGCCACCACTGATGAGGAGCGGCAGCACCTACAGGAGGTTGGTCTCTTCCACCTGGGCGAGTTTG TCAATGTGTTCTGCCATGGCTCCCTAGTTATGCAGAATCTGGGCGAGGCTTCTACCCCTACACAGGGCTC GGTGCTCTTTGGCACAGTCAACGGCATGATAGGGCTGGTGACCTCGCTGTCAGAGAGCTGGTATAACCTC CTGTTGGACATGCAGAACCGACTCAATAAGGTCATCAAAAGCGTGGGCAAGATTGAGCACTCCTTCTGGA GATCCTTTCACACTGAGCGAAAGACAGAACCAGCCACAGGCTTCATCGATGGTGATCTGATTGAAAGTTT CCTAGATATCAGCCGCCCTAAGATGCAGGAGGTTGTGGCAAACTTGCAGTATGATGATGGCAGTGGTATG AAGCGGGAGGCAACTGCAGATGACCTCATCAAAGTCGTGGAGGAGCTAACTCGGATCCATTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_015735 |
| Insert Size | 3423 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_015735.1, NP_056550.1 |
| RefSeq Size | 4171 bp |
| RefSeq ORF | 3423 bp |
| Locus ID | 13194 |
| UniProt ID | Q3U1J4 |
| Gene Summary | Required for DNA repair. Binds to DDB2 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also appears to function as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. DCX(DDB2) (also known as DDB1-CUL4-ROC1, CUL4-DDB-ROC1 and CUL4-DDB-RBX1) may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. DCX(DDB2) also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. DCX(ERCC8) (the CSA complex) plays a role in transcription-coupled repair (TCR). May also play a role in ubiquitination of CDKN1B/p27kip when associated with CUL4 and SKP2 (By similarity). The DDB1-CUL4A-DTL E3 ligase complex regulates the circadian clock function by mediating the ubiquitination and degradation of CRY1 (PubMed:26431207). DDB1-mediated CRY1 degradation promotes FOXO1 protein stability and FOXO1-mediated gluconeogenesis in the liver (PubMed:28790135).[UniProtKB/Swiss-Prot Function] |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG211698 | Ddb1 (tGFP-tagged) - Mouse damage specific DNA binding protein 1 (Ddb1) |
CNY 9,220.00 |
|
| MR211698 | Ddb1 (Myc-DDK-tagged) - Mouse damage specific DNA binding protein 1 (Ddb1) |
CNY 7,936.00 |
|
| MR211698L3 | Lenti ORF clone of Ddb1 (Myc-DDK-tagged) - Mouse damage specific DNA binding protein 1 (Ddb1) |
CNY 10,260.00 |
|
| MR211698L4 | Lenti ORF clone of Ddb1 (mGFP-tagged) - Mouse damage specific DNA binding protein 1 (Ddb1) |
CNY 10,260.00 |
