Tnks2 (NM_001163635) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	5430432P15Rik; AA517131; AI662480; ARTD6; Tank2; TNKS-2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC223799 representing NM_001163635 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGGCCGCCGCTGCGCCGGCGGGGGCGCGGCCTGCGCGAGCGCCGGGGCCGAGGCCGTGGAGCCGT CCGCCCGCGAGCTGTTCGAGGCTTGCCGCAACGGGGACGTGGAGCGAGTCAAGAGGCTGGTGACGCCCGA GAAGGTGAACAGCCGCGACACGGCGGGCAGGAAGTCCACCCCGCTCCACTTCGCCGCAGGTTTTGGACGG AAGGATGTAGTTGAATATCTTCTTCAGAACGGTGCAAATGTGCAAGCGCGTGATGATGGGGGTCTTATTC CTCTTCATAACGCATGCTCTTTTGGTCATGCTGAAGTAGTCAATCTTCTTTTACAACACGGTGCAGACCC AAATGCTCGGGATAATTGGAATTATACTCCTCTCCATGAAGCTGCAATTAAAGGAAAAATTGATGTTTGC ATTGTGCTTTTACAGCATGGAGCTGAGCCAACCATCCGAAATACAGATGGAAGGACAGCATTGGATTTAG CAGACCCATCTGCCAAAGCTGTTCTGACTGGTGACTATAAGAAAGATGAACTTCTGGAAAGTGCCAGGAG TGGCAATGAGGAAAAAATGATGGCCCTGCTTACACCATTGAATGTCAACTGCCATGCAAGTGATGGCAGA AAGTCAACTCCATTGCATTTGGCAGCAGGATACAACAGAGTAAAAATTGTACAACTGTTACTGCACCATG GGGCTGATGTCCATGCTAAAGATAAAGGGGATCTGGTACCACTACACAATGCCTGCTCTTACGGTCATTA TGAAGTAACTGAACTTCTGGTCAAGCACGGTGCCTGTGTGAATGCAATGGACCTGTGGCAGTTCACTCCT CTTCATGAGGCAGCTTCTAAGAACAGGATTGAAGTATGTTCTCTCCTCCTAAGTTACGGGGCAGATCCAA CCCTGCTGAACTGTCACAATAAAAGTGCTATAGACCTGGCCCCCACGGCACAGCTGAAGGAAAGATTATC GTATGAATTTAAAGGCCATTCATTGCTGCAAGCTGCACGAGAAGCTGATGTTACAAGAATCAAAAAACAT CTTTCTCTGGAAATGGTGAATTTCAAACATCCTCAGACACATGAAACAGCACTGCATTGTGCTGCTGCAT CTCCATATCCCAAAAGAAAGCAGATATGTGAACTGCTGCTCAGAAAGGGAGCAAACACCAACGAAAAGAC TAAAGAATTCTTGACTCCTCTGCACGTGGCTTCTGAGAACGCTCACAATGATGTTGTTGAAGTAGTGGTG AAACATGAAGCAAAGGTTAATGCTTTGGATAGTCTTGGACAGACGTCATTACACAGAGCTGCACACTGTG GTCACCTGCAGACCTGCCGCCTGCTCCTGAGCTATGGGTGTGATCCAAACATCATATCCCTTCAGGGTTT CACCGCCTTGCAAATGGGAAATGAAAATGTGCAGCAGCTGCTCCAAGAGGGCGCCTCACTAGGTCACTCA GAGGCGGACAGACAGCTGCTGGAAGCTGCAAAGGCTGGTGACGTTGAAACTGTAAAAAAACTCTGTACTG TTCAGAGTGTCAACTGCAGAGACATTGAAGGACGCCAGTCAACCCCACTCCACTTTGCAGCTGGGTACAA CAGAGTGTCTGTGGTAGAATATCTGCTGCAACATGGAGCTGATGTTCATGCTAAAGACAAAGGAGGTCTT GTACCTTTACACAATGCATGTTCTTATGGACACTATGAAGTTGCAGAACTTCTTGTCAAGCATGGAGCAG TAGTCAATGTCGCTGACTTGTGGAAGTTCACACCTTTACATGAAGCTGCAGCAAAAGGAAAATATGAAAT ATGCAAACTTCTACTCCAGCATGGTGCAGACCCTACAAAGAAAAACAGAGATGGTAATACTCCTCTGGAT CTTGTTAAAGATGGAGATACAGATATTCAAGATCTACTTAGAGGTGATGCAGCTTTGCTGGATGCTGCCA AGAAGGGTTGTTTAGCCAGAGTGAAGAAATTGTCTTCTCCTGATAATGTAAATTGCCGTGATACCCAAGG TCGGCATTCTACACCTTTACATTTGGCAGCTGGTTATAATAACTTGGAAGTTGCTGAGTATTTATTGCAA CATGGAGCTGATGTGAATGCCCAGGACAAAGGAGGACTCATTCCTTTACATAACGCAGCATCGTATGGAC ATGTAGATGTAGCAGCTTTGCTGATAAAGTACAATGCGTGTGTCAATGCCACGGACAAATGGGCTTTCAC ACCGTTACATGAAGCAGCCCAAAAGGGACGGACACAGCTTTGTGCTTTATTGTTGGCCCATGGAGCTGAT CCTACTCTTAAAAATCAAGAAGGACAAACACCTTTAGATTTAGTTTCAGCAGATGATGTCAGTGCACTCT TGACAGCAGCCATGCCCCCCTCTGCTCTGCCTACGTGCTACAAACCTCAAGTGCTCAGTGGCGTGAGGGG CCCCGGAGCCACTGCAGATGCTCTGTCTTCAGGTCCGTCCAGCCCATCCAGCCTCTCTGCAGCCAGCAGC CTCGACAACTTATCTGGCAGCTTCTCGGAACTGTCCGCAGTGGTTAGTTCAAGTGCAGCAGAAGGTGCTA CTGGTTTGCAAAGAAAAGAGGATTCAGGAATCGATTTTAGTATAACTCAGTTCATAAGGAACCTTGGACT TGAGCACTTGATGGATATATTTGAGAGAGAACAGATTACCTTAGATGTCCTAGTTGAAATGGGCCACAAG GAACTGAAAGAGATTGGAATCAATGCCTATGGACATCGACACAAGCTGATTAAAGGAGTTGAAAGGCTGA TCTCTGGACAACAAGGTCTTAATCCATATTTAACTCTAAACAACTCTGGTAGTGGAACAATTCTCATAGA TCTGTCTCCTGATGATAAAGAATTTCAGTCTGTGGAAGAAGAGATGCAGAGTACTGTGCGGGAGCACAGA GATGGTGGTCACGCAGGCGGCGTCTTCAACAGATACAACATTCTCAAGATTCAGAAGGTTTGTAACAAGA AATTGTGGGAAAGATATACACACCGGAGAAAAGAAGTTTCTGAAGAAAACCACAACCATGCAAACGAAAG GATGTTATTTCATGGGTCTCCTTTTGTGAATGCGATTATCCATAAGGGCTTTGATGAAAGGCATGCATAC ATAGGTGGCATGTTTGGAGCTGGAATTTATTTTGCTGAGAACTCTTCCAAAAGCAATCAATATGTGTATG GAATTGGAGGTGGCACCGGATGTCCAATTCACAAAGACAGATCGTGTTACATTTGTCACAGGCAGCTGCT GTTTTGTCGAGTAACCTTGGGAAAGTCTTTCTTGCAGTTCAGCGCAATGAAAATGGCACATTCTCCTCCC GGCCATCACTCGGTCACTGGCCGGCCCAGTGTAAATGGCCTAGCATTAGCTGAATATGTTATTTACAGAG GAGAACAGGCTTATCCTGAATATCTAATTACTTACCAGATTGTAAGGCCTGAAGGTATGGTTGATGGCTG A ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001163635
Insert Size	3501 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001163635.1, NP_001157107.1
RefSeq Size	6503 bp
RefSeq ORF	3501 bp
Locus ID	74493
UniProt ID	Q3UES3
Gene Summary	Poly-ADP-ribosyltransferase involved in various processes such as Wnt signaling pathway, telomere length and vesicle trafficking. Acts as an activator of the Wnt signaling pathway by mediating poly-ADP-ribosylation of AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex: poly-ADP-ribosylated target proteins are recognized by RNF146, which mediates their ubiquitination and subsequent degradation. Also mediates poly-ADP-ribosylation of BLZF1 and CASC3, followed by recruitment of RNF146 and subsequent ubiquitination. Mediates poly-ADP-ribosylation of TERF1, thereby contributing to the regulation of telomere length. Stimulates 26S proteasome activity.[UniProtKB/Swiss-Prot Function]

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