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Ehmt2 (NM_147151) Mouse Untagged Clone
CAT＃: MC223836
Ehmt2 (untagged) - Mouse euchromatic histone lysine N-methyltransferase 2 (Ehmt2), transcript variant short, (10ug)
CNY 15,110.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | Bat8; D17Ertd710e; G9a; KMT1C; NG36 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC223836 representing NM_147151
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGGCGGCGGGAGCTGCTGCGGCGGCGGCCGCCGAGGGGGAGGCCCCCGCTGAGATGGGGGCGC TGCTGCTGGAGAAGGAGCCCCGAGGAGCCGCCGAGAGAGTTCATAGCTCTTTGGGGGACACCCCTCAGAG TGAGGAGACCCTTCCCAAGGCCAACCCCGACTCCTTGGAGCCTGCCGGCCCCTCCTCTCCGGCCTCTGTC ACTGTCACCGTCGGCGATGAGGGGGCTGACACCCCTGTCGGGGCCGCATCACTCATCGGGGACGAACCCG AGAGCCTGGAGGGAGATGGGGGTCGCATCGTGCTGGGCCATGCCACAAAGTCGTTCCCCTCTTCCCCCAG CAAGGGGGGTGCCTGTCCCAGTCGGGCCAAAATGTCAATGACAGGGGCAGGAAAGTCGCCCCCCTCGGTC CAGAGTTTGGCCATGAGGCTGTTGAGCATGCCCGGGGCCCAGGGAGCTGCAACTGCTGGGCCTGAACCCT CTCCGGCAACAACTGCCGCCCAGGAGGGGCAGCCCAAAGTGCACCGAGCCCGGAAAACCATGTCCAAACC TAGCAACGGACAGCCTCCAATCCCTGAGAAGCGGCCCCCTGAAGTCCAGCATTTCCGCATGAGTGATGAC ATGCATCTGGGGAAGGTGACTTCAGATGTGGCCAAAAGGAGGAAGCTGAACTCTGGTAGCCTGTCCGAGG ACTTGGGCTCTGCCGGGGGCTCAGGAGATATAATCCTGGAGAAGGGAGAGCCCAGGCCCCTGGAGGAGTG GGAGACGGTGGTGGGCGATGACTTCAGCCTGTACTATGATGCGTACTCTGTGGATGAGCGGGTGGACTCT GACAGCAAGTCTGAAGTCGAAGCTCTAGCTGAACAGTTGAGTGAGGAGGAGGAGGAGGAAGAGGAGGAAG AAGAAGAAGAGGAGGAGGAGGAGGAAGAGGAGGAGGAGGAAGAAGAGGACGAGGAGTCGGGCAATCAGTC AGACAGGAGCGGTTCTAGTGGCCGGCGCAAGGCCAAGAAGAAATGGCGGAAAGACAGCCCGTGGGTGAAG CCATCTAGAAAACGGCGGAAACGAGAGCCTCCGAGGGCCAAGGAGCCAAGAGGGGTCTCCAATGACACGT CTTCACTGGAGACAGAACGCGGGTTTGAGGAGCTGCCCCTCTGCAGCTGCCGCATGGAGGCTCCCAAGAT TGACCGCATCAGCGAGAGAGCAGGGCACAAGTGCATGGCCACAGAGAGTGTGGATGGAGAGCTCCTGGGC TGCAATGCTGCCATCCTTAAGCGGGAGACCATGCGGCCGTCTAGCCGCGTGGCGCTGATGGTGCTCTGTG AGGCCCATCGAGCCCGCATGGTCAAGCACCATTGCTGCCCGGGCTGCGGCTACTTCTGCACAGCGGGCAC CTTCCTGGAATGCCACCCCGACTTTCGTGTAGCTCACCGCTTCCATAAGGCCTGCGTATCCCAGCTCAAT GGGATGGTCTTCTGTCCCCACTGTGGAGAGGATGCCTCAGAGGCCCAGGAGGTGACCATTCCTCGGGGCG ATGGGGGAACACCCCCAATTGGCACCGCAGCTCCTGCTCTGCCACCCCTGGCACATGATGCCCCAGGGCG AGCGGATACCTCCCAGCCTAGCGCCCGAATGCGAGGGCATGGAGAGCCGCGGCGCCCGCCCTGTGATCCC CTGGCTGACACCATCGACAGCTCAGGGCCTTCACTGACTCTGCCTAATGGGGGCTGCCTCTCCGCTGTGG GTCTGCCCCCAGGGCCGGGCAGGGAAGCCCTGGAAAAAGCCTTGGTCATCCAGGAGTCTGAGAGGCGGAA GAAGCTGCGATTCCACCCACGGCAGCTGTACCTGTCGGTGAAGCAGGGGGAGCTGCAGAAGGTGATCCTT ATGCTGTTAGACAACCTGGACCCCAACTTCCAGAGCGACCAGCAGAGCAAGCGCACGCCCCTGCACGCGG CCGCCCAGAAGGGGTCGGTAGAGATCTGTCATGTGCTGCTGCAGGCAGGAGCCAACATCAATGCCGTAGA TAAGCAACAACGCACGCCACTAATGGAGGCCGTGGTGAACAACCACCTGGAGGTGGCACGCTACATGGTG CAGTTAGGTGGCTGTGTCTACAGCAAGGAAGAGGATGGCTCCACCTGTCTACATCATGCAGCCAAAATTG GGAACTTGGAAATGGTCAGCCTGCTACTGAGCACAGGACAGGTGGACGTCAATGCCCAGGACAGTGGGGG CTGGACGCCCATCATCTGGGCAGCCGAGCACAAGCACATCGATGTGATTCGTATGCTGCTGACCCGGGGT GCCGATGTCACCCTGACTGACAATGAGGAAAACATCTGCCTGCACTGGGCCTCCTTCACGGGTAGTGCCG CCATCGCTGAGGTCCTTCTGAATGCCCAGTGTGATCTCCATGCTGTCAACTACCATGGGGACACGCCCCT GCACATAGCCGCCAGGGAGAGCTACCATGACTGTGTTCTGTTGTTCCTGTCTCGTGGAGCCAACCCTGAG CTTCGGAACAAAGAAGGAGACACGGCATGGGATCTGACCCCAGAGCGCTCTGATGTGTGGTTTGCACTGC AGCTCAATCGAAAGCTTAGGCTTGGGGTAGGGAACCGGGCTGTCCGCACCGAGAAGATCATCTGCCGGGA CGTAGCCCGAGGCTATGAGAATGTACCCATCCCCTGTGTCAATGGTGTGGATGGGGAGCCGTGCCCGGAG GACTACAAGTACATCTCTGAGAACTGCGAGACATCGACCATGAACATCGACCGCAACATCACCCATCTGC AGCACTGCACGTGTGTGGATGACTGCTCCAGCTCCAATTGCCTATGTGGTCAGCTCAGTATCCGATGCTG GTATGACAAGGACGGGCGGCTGCTCCAGGAGTTTAACAAGATCGAGCCCCCCCTGATCTTTGAGTGTAAC CAGGCATGCTCCTGCTGGAGAAGCTGCAAGAACCGCGTGGTGCAGAGCGGCATCAAGGTACGGCTGCAGC TCTACCGGACTGCCAAGATGGGCTGGGGGGTCCGAGCCTTGCAGACCATCCCCCAGGGCACGTTCATCTG CGAGTATGTAGGAGAGCTGATCTCTGATGCCGAGGCTGATGTGAGAGAGGATGATTCTTACCTCTTCGAT TTAGATAACAAGGATGGCGAGGTTTACTGCATTGATGCCCGTTACTATGGCAACATCAGCCGATTCATTA ACCACCTGTGTGACCCCAACATCATCCCTGTCCGGGTTTTCATGCTGCACCAAGATCTACGGTTCCCACG CATTGCCTTCTTCAGCTCCAGGGACATCCGGACTGGGGAGGAGCTGGGCTTTGACTACGGTGACCGATTC TGGGACATCAAGAGCAAGTATTTCACCTGCCAGTGTGGCTCTGAGAAGTGCAAGCATTCAGCGGAGGCCA TCGCCCTGGAGCAGAGCCGCCTGGCCCGGCTGGACCCCCACCCGGAGCTGCTCCCTGACCTCAGCTCCCT GCCCCCCATCAACACCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_147151 |
| Insert Size | 3519 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_147151.2, NP_671493.1 |
| RefSeq Size | 3805 bp |
| RefSeq ORF | 3519 bp |
| Locus ID | 110147 |
| UniProt ID | Q9Z148 |
| Gene Summary | Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also mediates monomethylation of 'Lys-56' of histone H3 (H3K56me1) in G1 phase, leading to promote interaction between histone H3 and PCNA and regulating DNA replication. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. May also methylate histone H1. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53. Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) has an alternate 5' terminal exon which includes the 5' coding sequence, and lacks an in-frame internal coding exon, compared to variant 1. The resulting isoform (b, also known as G9a short) has a shorter and distinct N-terminus and lacks an internal segment, compared to isoform a. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG222625 | Ehmt2 (tGFP-tagged) - Mouse euchromatic histone lysine N-methyltransferase 2 (Ehmt2) transcript variant short, (10ug) |
CNY 12,830.00 |
|
| MR222625 | Ehmt2 (Myc-DDK-tagged) - Mouse euchromatic histone lysine N-methyltransferase 2 (Ehmt2), transcript variant short |
CNY 11,690.00 |
|
| MR222625L3 | Lenti ORF clone of Ehmt2 (Myc-DDK-tagged) - Mouse euchromatic histone lysine N-methyltransferase 2 (Ehmt2), transcript variant short |
CNY 13,590.00 |
|
| MR222625L4 | Lenti ORF clone of Ehmt2 (mGFP-tagged) - Mouse euchromatic histone lysine N-methyltransferase 2 (Ehmt2), transcript variant short |
CNY 13,590.00 |
