Aff1 (NM_133919) Mouse Untagged Clone

CAT#: MC223967

Aff1 (untagged) - Mouse AF4/FMR2 family, member 1 (Aff1), transcript variant 2, (10ug)



  "NM_133919" in other vectors (4)

CNY 15,580.00


货期*
7周

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit Polyclonal Anti-Aff1 Antibody
    • 100 ul

CNY 5,250.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 9630032B01Rik; Af; Af4; AW319193; Mllt; Mllt2h; R; Rob
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC223967 representing NM_133919
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGCCCATTCCAGCTTGTACAATGAAGATAGAAACCTGCTTCGAATCAGAGAGAAGGAAAGACGCA
ACCAAGAAGCTCACCAGGAGAAGGAGGCATTTCCCGAGAAGGCTCCCCTGTTTCCAGAGCCTTACAAGAC
TGCAAAAGGCGATGAGCTATCAAGTCGGATCCAGACCATGCTGGGTGACTATGAGGAGATGAAGGAGTTC
CTGAGCAGCAAGTCCCACCCCCACCGCCTGGATGGCTCTGAAGACAGGCCCGGGAAGCCCAGATATCCCT
TAGGTCATGACAGGGGGAACGGGGCTGCATCCAGCTCCCTCCGCACACATGTCTACCACCAGCCTATCCA
CACTTCTGCTCCCGGATCACGTCCTGTCGGTAACATTAGCCACAGTCCAAAGATGGCACAGCCAAGGATG
GAGCCAAGTCTCCACACCAAAATCTATGATGGCCCACGTCTGACTCAAGACCACCTCAGTCAGGGACATT
GTTCCAGAAAGTGTGACCGAAGAGCTGAAGGAGACTCTGCTCCCGAGAGGAAGCTCTCGCCCTTGATCTC
CTCTTTGCCATCCCCGGTGCCCCCTCTATCACCTGTACATTCCAGGCTGCAGGGAACCAGCAAGGCTCAC
AGCAGTGGCGTTAGCAGTAAAAGCTGCTGTGTGGCCAAGTCTTCCAAGGACTTGGTGGCGAAGGCCCAAG
ATAAAGAGACTCCTCATGACGGTTTGGTGGCAGTTACCAGCCTTGGATCAGCCCCTCCTCAGCCACCTTG
CCAGACATTTCCACCCCCTCCTCTCCCCTCAAAAAGTGCTGCAATGCAGCAGAAGCCCACGGCATATGTC
CGTCCCATGGACGGTCAGGATCAGGCTCCCAGTGAGTCCCCCGAGCTGAAGCTGCCACTGGAGGACTATG
GTCAGCAAAGCTTTGAGAAACCAGACCTTAAAGTGCCTGCCAAAGCCAAGCTCACCAGACTAAGGATGCC
CTCTCAGTCGGTGGAGCAGCCGTACTCCAATGAAGTCCATTGCGTTGAAGAGATTCTGAAGGAAATGACC
CACTCGTGGCCACCGCCTCTGACAGCCATACACACACCCAGTACAGCCGAGCCGTCCAGATTTCCTTTCC
CCACAAAGGACCCTCTGCACGTCAGTCCTGCAACCCAGAGCCAAAAACAATATGACACGCCTTCAAAAAC
CCACCCCAATCCCCAGCAAGGAACCTCCATGCTGGAGGACGACCTGCAGCTCAGCGACAGTGAAGACAGC
GACACGGAGCAAGCCACAGAGAAGCCTCCATCCCCACCTGCACCTCCCAGCGCTCCGCAGACACTTCCCG
AGCCCGTGGCCTCAGCACATTCCAGTAGTGGGGAGTCAGAGAGCAGCGAGTCAGACAGTTCCTCAGACTC
TGAGAGTGAGAGCAGCTCCAGCGACAGCGAGGAGGAGGAAGAGAACGAGCCCCTGGAGACCCGGGCTCCC
GAGCCCGAGCCTCCGACGACAAACAAATGGCAGCTGGACAACTGGTTGACCAAAGTCAACCAACCCTCAG
TGCCCCTGGATGGCCGGGGGAGCACAGAGTCTCCACAATGGCGCCAGGAAAGTAAGGGTGTCGCTGAGGG
CAGCAGTGACCAGCAGCATCCTGATTCCAAAGACCCTCTCCCAAAGAGCTCCAGCAAGACTCTCCGGGGT
CCCTCTGAGGGGCCCCAGCCTGGGAAGAGGGGCTGTCCGAAATCCCCCGCTCAACAGGAACCCCCACCTC
GGCAAACCGTGGGCAGCAAACAACCCAGGAAACCCGCCAAGGGCTCCGGCCAGGCAGAGCCCCAGGCCAG
CTCGCAGGCAGAGAGTGAAGTAGGACCCCTTCCCTACGGCTCAAAGGAGCAGACTTCCAAAGACAGACCC
AAGGTGAAGACGAAAGGCAGGCCGCGTGCCGTGGGCAGTCGGGAGCCTAAGCCAGAGGTCCCTGCGCCCA
CGCCTCAGGCAGCCGTGCCCAGACCGAAGCCGCCTGTGCCCACCCCCAGTGAGAAGAGGAAGCACAAGAG
CTCCACGGCCCCCTCCAAGGCGCCCTCAGCCCCCCAGCCTCCGAAGGACAGTGCTGGGGACAGGAACCCA
GAACACTCTGCTCTTGTCTCCTTGACTCAGAGCCAGGGTCCATCCCACAGCAGCAGGGGCAGCAGTGGCA
GCGTCAGGACTAGTGGCTGCCGACAGGCTGTGATTGCCCAAGGGGACGGCTGTAAAGACAAGCTCCTGTT
GCCTTTGAGAGACACCAAATTGCTCTCGCCGCTCAGGGACTCCCCACCACCGACAAGCTTGGTGGTGAAG
ATAACCCTAGACCTCCTCACGAGGATTCCCCAGCCTCTGGGGAAGGGGAGCCGCCCCAGGAAAGCAGAAG
ACAAGCAGCTGTCAGCAGGGAAGAAGCAGGACTCGGAGACCAAGAGCTGTGACAGCTCCAGCAGAGTGAC
CAAGAAGAGAAAGGGTGACCCAGAAAAAGAGCACAGTAACAAGAGACACAAACTGGATAAGTCGCAGACT
GCGTCATCTTCATCTTCCCATACAGAATCTTCCAGAACAAAAGCGCCCAGGTCCTCCTCAGAGAACTCAA
GAAAGGAAATGCTGCCTCCAGCATCGGCGTCCTCAGTCTCCTCTTCCTCATCCTCCCAGAAGCCAAGCAG
ACCTGCACAGAAGAGGCCAAGGCCAGACGAAGACACGTGTAGCCAGGAACCCCCCAGAAGTGCCAGCAGC
ACAAAGAGCAGCTCTACAGACCCTCCCGCTCCCAAGCACAGAAAAGTACAGGCCAGGGGCTCTGAACACA
AGGGATCTTCTGGGGATGCTGCGAATGCTGCAAATCCTTTTCCAGTGCCTTCTTTGCCGAATGGTAACGC
TAAACCAGGGAAGCCACAGGTGAAGTCTGACAGACAACAAGCTGACTTTCACATGAAGGAGGCTAAAAAG
CTGAAGTGCAAAGCAGAGACAATGGTAGACAAGGCTGGAAAAGCCTTCAAGTATTTGGAAGCCGTCCTCT
CTTTTATCGAGTGTGGAATGGCCTCAGAGTCAGAAAGCTCAGCCAAGTCGGCGTACGCTGTGTACTCAGA
AACCATAGACCTCATTAGGTATGTGATGTCACTAAAATGCTTCTCAGATAACACGATGCCAGCACAAGAG
AAGATATTTGCTGTTTTATGCTTGCGTTGCCAGTCCCTTTTGAACATGGCGATGTTTCGATGTAAGAAAG
ACACAGTGATGAAGTATTCTCGTACTCTCAGTGAACACTTCAAGAGTACTTCCAAAGTGGCCCAGGCACC
TTCTCCATGCACGGCAAGAAGCACAGGCGTTCCGTCCCCCCTCTCCCCAATGCCTTCTCCTGCCAGCTCC
GTGGGGTCCCAATCAAGTGCTGGCAGCAGTATGGGGAGCGTCGGGGTGACGGCCACCGTCAGTACTCCAG
TCTCCATCCAGAACATGACCTCCTCCTATGTCACCATCACATCCCATGTCCTTACGGCCTTTAGCCTTTG
GGAACAGGCCGAGGCCCTCACAAGGAAGAACAAAGAATTCTTCGCTCAGCTCAGCACAAAAGTGCGTGTG
TTGGCCCTCAACAGCAGCCTGGTGGACCTGGTGCACTACACAAGACAGGGCCTGCAGCGGCTAAAACAGT
CACCCAAAGGCCCCTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_133919
Insert Size 3657 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_133919.4, NP_598680.3
RefSeq Size 8339 bp
RefSeq ORF 3657 bp
Locus ID 17355
Gene Summary This gene encodes a member of the AF4/ lymphoid nuclear protein related to the Fragile X E syndrome (FRAXE) family of proteins, which have been implicated in human childhood lymphoblastic leukemia, fragile chromosome X intellectual disability, and ataxia. It is the prevalent mixed-lineage leukemia fusion gene associated with spontaneous acute lymphoblastic leukemia. Members of this family have three conserved domains: an N-terminal homology domain, an AF4/ lymphoid nuclear protein domain, and a C-terminal homology domain. Knockout of the mouse gene by homologous recombination severely affects early events in lymphopoiesis, including precursor proliferation or recruitment, but is dispensable for terminal differentiation. In addition, an autosomal dominant missense mutation results in several phenotypes including ataxia and adult-onset Purkinje cell loss in the cerebellum, indicating a role in Purkinje cell maintenance and function. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2017]
Transcript Variant: This variant (2) uses an alternate 5'-terminal exon, which results in a different 5' UTR and use of an alternate start codon. It encodes isoform 2, which has a distinct N-terminus compared to isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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