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Phka1 (NM_008832) Mouse Untagged Clone
CAT＃: MC224028
Phka1 (untagged) - Mouse phosphorylase kinase alpha 1 (Phka1), transcript variant 1, (10ug)
CNY 15,870.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 5330411D17; 9830108K24Rik; Phka |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC224028 representing NM_008832
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGAGCCGCAGTAACTCGGGAGTCCGCCTGGACGGCTATGCTCGGCTGGTGCATCAGACCATCCTGT GCCATCAGAATCCAGTGACAGGCTTGCTTCCAGCCAGCTATGATCAAAAAGATGCCTGGGTCCGAGATAA TGTGTACAGTATCCTGGCTGTGTGGGGTTTGGGCCTGGCATATCGCAAAAATGCTGACCGTGATGAGGAC AAGGCAAAGGCCTATGAACTGGAGCAGAGTGTAGTGAAGTTAATGAGGGGACTGCTGCACTGCATGATCA GACAGGTGGATAAAGTAGAGTCCTTCAAGTACAGTCAGAGTACTAAAGATAGCCTCCATGCCAAGTACAA CACCAAAACTTGTGCCACTGTGGTGGGTGATGACCAGTGGGGACACCTGCAGTTGGATGCTACTTCTGTG TACCTGCTCTTCCTAGCACAAATGACTGCCTCAGGACTCCATATCATCCACAGCCTAGACGAAGTCAATT TTATACAGAACCTCGTGTTTTACATTGAAGCTGCATATAAAACTGCTGACTTTGGGATATGGGAACGTGG CGATAAGACAAACCAAGGCATCTCGGAATTGAATGCGAGTTCAGTTGGAATGGCCAAGGCAGCCCTGGAA GCACTAGATGAATTAGACTTGTTTGGTGTGAAAGGTGGGCCACAATCAGTTATCCATGTCCTGGCTGATG AAGTCCAACACTGCCAGTCTATCCTGAATTCACTACTGCCCAGGGCTTCAACATCCAAAGAAGTTGATGC CAGTCTGCTCTCAGTGGTCTCTTTCCCAGCCTTTGCTGTAGAGGACAGCCATTTGGTGGAGCTCACCAAA CAGGAGATCATCACCAAGCTTCAGGGTCGTTATGGTTGCTGTCGTTTTCTGCGAGATGGATATAAAACTC CTAAAGAGGATCCCAATCGCCTATACTATGAACCAGCTGAGCTGAAGCTATTTGAAAACATTGAGTGCGA ATGGCCATTGTTCTGGACATACTTTATCCTTGATGGGATCTTCAGTGGCAACGTAGAACAGGTTCAAGAA TATAGAGAGGCTCTTGATGCAGTCCTCATCAAGGGCAAAAATGGAGTCCCTCTTCTTCCAGAGCTGTACA GTGTCCCTCCTGACAGGGTTGATGAAGAGTATCAAAATCCCCACACTGTGGATCGAGTCCCTATGGGAAA ATTGCCTCACATGTGGGGTCAGTCTCTATACATTTTAGGAAGCTTGATGGCAGAGGGATTTTTAGCTCCT GGAGAAATTGATCCCCTGAATCGTAGGTTTTCTACTGTGCCAAAGCCAGATGTGGTGGTTCAAGTCTCCA TTCTGGCTGAAACAGAAGAAATCAAGGCCATTTTGAAGGACAAAGGAATTGATGTGGAGACCATTGCTGA AGTGTACCCCATAAGAGTACAGCCAGCTCGTATTCTCAGCCATATTTATTCTAGTCTAGGATGCAACAGT AGAATGAAACTCAGTGGACGACCCTACAGGCTCATGGGTGTGCTTGGAACATCAAAACTTTATGACATTC GCAAAACTATCTTTACTTTCACTCCACAGTTTATAGACCAGCAACAGTTCTACCTGGCTCTGGACAACCA GATGATAGTAGAAATGCTCAGAACAGACCTTTCCTACCTCTGTAGCCGCTGGAGGATGACAGGCCAGCCC ACGATCACTTTCCCTATCTCGCACACCATGCTTGATGAAGATGGAACCAGCTTGAATTCAAGTATCTTGG CAGCACTCCGAAAAATGCAGGATGGCTATTTTGGTGGGGCCAGGATCCAAACAGGTAAATTGTCAGAGTT TTTGACAACATCTTGCTGCACACACTTAAGCTTCATGGACCCTGGACCTGAGGGTAAGCTGTACAGTGAA GATTATGATGAAGACTATGAAGATGATTTGGACTCTGGCAACTGGATGGACAGCTATGATTCAACAAGTA ATGCTCGCTGTGGTGATGAAGTTGCCCGTTATTTAGACCGCCTTTTGGCACACACTGTTCCCCATCCTAA ACTAGCTCCTACCTCACGGAAGGGAGGGCTAGATCGGTTCCGAGCTGCTGTGCAAACAACTTGCGACTTA ATGTCCTTGGTGGCCAAGGCCAAGGAGCTGCATATACAGAATGTTCACATGTATCTTCCTACAAAGTTAT TTCAGCCTTCTCGTCCTTCACTCAACTTACTTGACTCCCCTGAGTCTCCACAAGATAGCCAGGTTCCTTC CGTTCATGTAGAAGTGCATCTTCCTAGGGACCAGTCTGGGGAAGTGGACTTCCAGTCATTGGTTTCACAG TTGAAGGAGACCTCAAGCTTACAGGAGCAAGCTGATATACTCTACATGCTGTATTCAATGAAAGGACCTG ACTGGAACACTGAATTGTATGAAGAAGGGGGGGCTACTGTCAGAGAGCTTCTTAGTGAACTGTATGTCAA AGTTGGTGAAATTCGGCACTGGGGTCTGATCCGATATATCTCTGGGATCTTACGGAAGAAAGTGGAGGCA CTTGATGAGGCCTGCACAGACCTTCTGTCCTACCAGAAACACCTGACAGTAGGACTTCCTCCAGAACCTC GAGAGAAGACCATCTCTGCGCCTCTACCGTACGAGGCACTCACTAAGCTGATAGATGAAGCCAGTGAAGG CGACATGAGCATCTCAACCCTTACACAGGAAATAATGGTCTATCTTGCCATGTATATGAGAACTCAGCCT GGCCTCTTTGCAGAAATGTTCAGACTTCGAATCGGTTTGATCATACAAGTTATGGCAACAGAACTAGCAC ACTCTCTTCGATGTTCAGCTGAGGAAGCCACAGAGGGCCTGATGAATCTCAGTCCTTCAGCCATGAAGAA CCTCCTGCATCACATTCTCAGTGGCAAAGAGTTTGGAGTGGAACGAAGCGTTCGTCCCACTGATTCAAAT GTGAGTCCTGCTATTTCCATCCATGAGATTGGTGCTGTTGGAGCAACAAAAACTGAACGAACTGGAATCA TGCAGTTAAAAAGTGAGATAAAGCAGGTGGAATTTCGTAGGCTGTCTGTCTCGATGGAGAGTCAGACTAG TGGTGGTCATCCCTCGGGTGTAGATTTGATGTCGCCATCCTTTCTGTCCCCTGCAGCCTGTATTGCTGCA AGTAGTGGATCCTTTCCTACGGTGTGTGATCATCAGACATCTAAAGATAGTCGTCAAGGCCAGTGGCAAC GCAGGAGAAGGCTAGATGGAGCACTAAATAGAGTACCAATTGGATTTTATCAAAAAGTATGGAAAATTTT GCAGAAATGTCATGGGCTTTCTGTGGAAGGTTTTGTTCTTCCCTCTTCAACCACTAGGGAGATGACCCCA GGTGAGATTAAATTCTCTGTCCATGTGGAGTCTGTCCTGAATCGTGTCCCTCAGCCAGAATACCGCCAAC TTCTGGTTGAAGCCATCCTTGTTCTCACCATGCTGGCAGATATTGAAATTCATAGCATTGGGAGCATCAT TGCTGTGGAGAAAATAGTTCATATTGCCAACGACTTGTTCCTTCAAGAACAGAAAACCCTCGGCGCAGAT GATACCATGTTGGCAAAGGATCCTGCATCTGGCATCTGTACTCTCCTGTATGACAGTGCACCCAGTGGCA GATTTGGCACCATGACCTACCTCTCCAAGGCAGCTGCCACCTACGTGCAGGAGTTTCTGCCACACAGCCT CTGTGCCATGCAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_008832 |
| Insert Size | 3726 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_008832.2, NP_032858.2 |
| RefSeq Size | 6115 bp |
| RefSeq ORF | 3726 bp |
| Locus ID | 18679 |
| UniProt ID | P18826 |
| Gene Summary | Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The alpha chain may bind calmodulin.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG226483 | Phka1 (tGFP-tagged) - Mouse phosphorylase kinase alpha 1 (Phka1) transcript variant 1, (10ug) |
CNY 13,590.00 |
|
| MR226483 | Phka1 (Myc-DDK-tagged) - Mouse phosphorylase kinase alpha 1 (Phka1), transcript variant 1 |
CNY 12,350.00 |
|
| MR226483L3 | Lenti ORF clone of Phka1 (Myc-DDK-tagged) - Mouse phosphorylase kinase alpha 1 (Phka1), transcript variant 1 |
CNY 14,250.00 |
|
| MR226483L4 | Lenti ORF clone of Phka1 (mGFP-tagged) - Mouse phosphorylase kinase alpha 1 (Phka1), transcript variant 1 |
CNY 14,250.00 |
