Phka1 (NM_008832) Mouse Untagged Clone

CAT#: MC224028

Phka1 (untagged) - Mouse phosphorylase kinase alpha 1 (Phka1), transcript variant 1, (10ug)



  "NM_008832" in other vectors (4)

CNY 15,870.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 5330411D17; 9830108K24Rik; Phka
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC224028 representing NM_008832
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGGAGCCGCAGTAACTCGGGAGTCCGCCTGGACGGCTATGCTCGGCTGGTGCATCAGACCATCCTGT
GCCATCAGAATCCAGTGACAGGCTTGCTTCCAGCCAGCTATGATCAAAAAGATGCCTGGGTCCGAGATAA
TGTGTACAGTATCCTGGCTGTGTGGGGTTTGGGCCTGGCATATCGCAAAAATGCTGACCGTGATGAGGAC
AAGGCAAAGGCCTATGAACTGGAGCAGAGTGTAGTGAAGTTAATGAGGGGACTGCTGCACTGCATGATCA
GACAGGTGGATAAAGTAGAGTCCTTCAAGTACAGTCAGAGTACTAAAGATAGCCTCCATGCCAAGTACAA
CACCAAAACTTGTGCCACTGTGGTGGGTGATGACCAGTGGGGACACCTGCAGTTGGATGCTACTTCTGTG
TACCTGCTCTTCCTAGCACAAATGACTGCCTCAGGACTCCATATCATCCACAGCCTAGACGAAGTCAATT
TTATACAGAACCTCGTGTTTTACATTGAAGCTGCATATAAAACTGCTGACTTTGGGATATGGGAACGTGG
CGATAAGACAAACCAAGGCATCTCGGAATTGAATGCGAGTTCAGTTGGAATGGCCAAGGCAGCCCTGGAA
GCACTAGATGAATTAGACTTGTTTGGTGTGAAAGGTGGGCCACAATCAGTTATCCATGTCCTGGCTGATG
AAGTCCAACACTGCCAGTCTATCCTGAATTCACTACTGCCCAGGGCTTCAACATCCAAAGAAGTTGATGC
CAGTCTGCTCTCAGTGGTCTCTTTCCCAGCCTTTGCTGTAGAGGACAGCCATTTGGTGGAGCTCACCAAA
CAGGAGATCATCACCAAGCTTCAGGGTCGTTATGGTTGCTGTCGTTTTCTGCGAGATGGATATAAAACTC
CTAAAGAGGATCCCAATCGCCTATACTATGAACCAGCTGAGCTGAAGCTATTTGAAAACATTGAGTGCGA
ATGGCCATTGTTCTGGACATACTTTATCCTTGATGGGATCTTCAGTGGCAACGTAGAACAGGTTCAAGAA
TATAGAGAGGCTCTTGATGCAGTCCTCATCAAGGGCAAAAATGGAGTCCCTCTTCTTCCAGAGCTGTACA
GTGTCCCTCCTGACAGGGTTGATGAAGAGTATCAAAATCCCCACACTGTGGATCGAGTCCCTATGGGAAA
ATTGCCTCACATGTGGGGTCAGTCTCTATACATTTTAGGAAGCTTGATGGCAGAGGGATTTTTAGCTCCT
GGAGAAATTGATCCCCTGAATCGTAGGTTTTCTACTGTGCCAAAGCCAGATGTGGTGGTTCAAGTCTCCA
TTCTGGCTGAAACAGAAGAAATCAAGGCCATTTTGAAGGACAAAGGAATTGATGTGGAGACCATTGCTGA
AGTGTACCCCATAAGAGTACAGCCAGCTCGTATTCTCAGCCATATTTATTCTAGTCTAGGATGCAACAGT
AGAATGAAACTCAGTGGACGACCCTACAGGCTCATGGGTGTGCTTGGAACATCAAAACTTTATGACATTC
GCAAAACTATCTTTACTTTCACTCCACAGTTTATAGACCAGCAACAGTTCTACCTGGCTCTGGACAACCA
GATGATAGTAGAAATGCTCAGAACAGACCTTTCCTACCTCTGTAGCCGCTGGAGGATGACAGGCCAGCCC
ACGATCACTTTCCCTATCTCGCACACCATGCTTGATGAAGATGGAACCAGCTTGAATTCAAGTATCTTGG
CAGCACTCCGAAAAATGCAGGATGGCTATTTTGGTGGGGCCAGGATCCAAACAGGTAAATTGTCAGAGTT
TTTGACAACATCTTGCTGCACACACTTAAGCTTCATGGACCCTGGACCTGAGGGTAAGCTGTACAGTGAA
GATTATGATGAAGACTATGAAGATGATTTGGACTCTGGCAACTGGATGGACAGCTATGATTCAACAAGTA
ATGCTCGCTGTGGTGATGAAGTTGCCCGTTATTTAGACCGCCTTTTGGCACACACTGTTCCCCATCCTAA
ACTAGCTCCTACCTCACGGAAGGGAGGGCTAGATCGGTTCCGAGCTGCTGTGCAAACAACTTGCGACTTA
ATGTCCTTGGTGGCCAAGGCCAAGGAGCTGCATATACAGAATGTTCACATGTATCTTCCTACAAAGTTAT
TTCAGCCTTCTCGTCCTTCACTCAACTTACTTGACTCCCCTGAGTCTCCACAAGATAGCCAGGTTCCTTC
CGTTCATGTAGAAGTGCATCTTCCTAGGGACCAGTCTGGGGAAGTGGACTTCCAGTCATTGGTTTCACAG
TTGAAGGAGACCTCAAGCTTACAGGAGCAAGCTGATATACTCTACATGCTGTATTCAATGAAAGGACCTG
ACTGGAACACTGAATTGTATGAAGAAGGGGGGGCTACTGTCAGAGAGCTTCTTAGTGAACTGTATGTCAA
AGTTGGTGAAATTCGGCACTGGGGTCTGATCCGATATATCTCTGGGATCTTACGGAAGAAAGTGGAGGCA
CTTGATGAGGCCTGCACAGACCTTCTGTCCTACCAGAAACACCTGACAGTAGGACTTCCTCCAGAACCTC
GAGAGAAGACCATCTCTGCGCCTCTACCGTACGAGGCACTCACTAAGCTGATAGATGAAGCCAGTGAAGG
CGACATGAGCATCTCAACCCTTACACAGGAAATAATGGTCTATCTTGCCATGTATATGAGAACTCAGCCT
GGCCTCTTTGCAGAAATGTTCAGACTTCGAATCGGTTTGATCATACAAGTTATGGCAACAGAACTAGCAC
ACTCTCTTCGATGTTCAGCTGAGGAAGCCACAGAGGGCCTGATGAATCTCAGTCCTTCAGCCATGAAGAA
CCTCCTGCATCACATTCTCAGTGGCAAAGAGTTTGGAGTGGAACGAAGCGTTCGTCCCACTGATTCAAAT
GTGAGTCCTGCTATTTCCATCCATGAGATTGGTGCTGTTGGAGCAACAAAAACTGAACGAACTGGAATCA
TGCAGTTAAAAAGTGAGATAAAGCAGGTGGAATTTCGTAGGCTGTCTGTCTCGATGGAGAGTCAGACTAG
TGGTGGTCATCCCTCGGGTGTAGATTTGATGTCGCCATCCTTTCTGTCCCCTGCAGCCTGTATTGCTGCA
AGTAGTGGATCCTTTCCTACGGTGTGTGATCATCAGACATCTAAAGATAGTCGTCAAGGCCAGTGGCAAC
GCAGGAGAAGGCTAGATGGAGCACTAAATAGAGTACCAATTGGATTTTATCAAAAAGTATGGAAAATTTT
GCAGAAATGTCATGGGCTTTCTGTGGAAGGTTTTGTTCTTCCCTCTTCAACCACTAGGGAGATGACCCCA
GGTGAGATTAAATTCTCTGTCCATGTGGAGTCTGTCCTGAATCGTGTCCCTCAGCCAGAATACCGCCAAC
TTCTGGTTGAAGCCATCCTTGTTCTCACCATGCTGGCAGATATTGAAATTCATAGCATTGGGAGCATCAT
TGCTGTGGAGAAAATAGTTCATATTGCCAACGACTTGTTCCTTCAAGAACAGAAAACCCTCGGCGCAGAT
GATACCATGTTGGCAAAGGATCCTGCATCTGGCATCTGTACTCTCCTGTATGACAGTGCACCCAGTGGCA
GATTTGGCACCATGACCTACCTCTCCAAGGCAGCTGCCACCTACGTGCAGGAGTTTCTGCCACACAGCCT
CTGTGCCATGCAGTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_008832
Insert Size 3726 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_008832.2, NP_032858.2
RefSeq Size 6115 bp
RefSeq ORF 3726 bp
Locus ID 18679
UniProt ID P18826
Gene Summary Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The alpha chain may bind calmodulin.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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