Grin2d (NM_008172) Mouse Untagged Clone

CAT#: MC224232

Grin2d (untagged) - Mouse glutamate receptor, ionotropic, NMDA2D (epsilon 4) (Grin2d), (10ug)



  "NM_008172" in other vectors (4)

CNY 17,010.00


货期*
14周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms GluN2D; NMDAR2D; NR2D
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC224232 representing NM_008172
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGCGGCGCCGGTGGCCCCCGCGGCCCTCGGGGCCCCGCTAAGATGCTGTTGCTGCTGGCGCTGGCGT
GCGCCAGCCCGTTCCCGGAGGAGGTGCCGGGGCCGGGCGCGGCCGGCGGGGGCACGGGCGGGGCGCGGCC
GCTCAACGTGGCGCTGGTCTTCTCTGGCCCGGCGTACGCGGCCGAGGCGGCGCGCTTGGGCCCGGCCGTG
GCGGCGGCAGTGCGCAGCCCGGGCCTGGACGTACGGCCCGTGGCGCTGGTGCTCAACGGCTCCGACCCTC
GCAGCCTTGTGCTGCAGCTCTGCGACCTGCTGTCGGGGCTGCGCGTGCACGGCGTGGTGTTCGAGGACGA
CTCGCGCGCGCCCGCCGTCGCGCCCATTCTCGACTTCCTGTCGGCGCAGACCTCGCTGCCCATCGTGGCC
GTGCACGGCGGCGCCGCGCTCGTACTCACACCCAAGGAGAAGGGCTCCACCTTCCTGCAGCTTGGCTCCT
CCACAGAGCAACAGCTGCAGGTCATTTTTGAGGTGCTGGAGGAGTACGACTGGACATCCTTTGTGGCAGT
GACTACGCGTGCCCCAGGCCATCGAGCCTTCTTGTCATACATCGAGGTGCTGACTGATGGCAGCCTGGTG
GGCTGGGAGCATCGAGGAGCGCTGACACTGGACCCCGGAGCGGGTGAGGCCGTCCTGGGCGCACAGCTCC
GTAGTGTCAGTGCGCAGATCCGCCTGCTCTTCTGCGCCCGCGAGGAGGCAGAGCCTGTTTTCCGGGCGGC
AGAAGAGGCTGGTCTCACTGGGCCTGGCTACGTCTGGTTCATGGTGGGACCTCAGCTGGCCGGAGGTGGG
GGCTCCGGGGTCCCTGGGGAACCACTTCTTCTGCCAGGAGGTGCCCCACTGCCTGCTGGGCTGTTTGCAG
TGCGCTCTGCTGGCTGGCGTGACGACTTGGCACGTCGAGTGGCTGCTGGTGTGGCGGTGGTGGCCAGAGG
TGCCCAGGCCCTGCTGCGAGACTATGGCTTCCTGCCTGAGCTGGGCCATGACTGTCGCGCCCAGAATCGC
ACCCACCGCGGGGAGAGTCTGCACAGGTATTTCATGAACATCACCTGGGATAACCGAGACTACTCCTTCA
ATGAGGATGGCTTTCTGGTAAACCCGTCACTGGTAGTCATCTCCCTCACCAGAGACAGGACGTGGGAAGT
GGTGGGCAGCTGGGAACAGCAGACCCTCCGCCTCAAGTACCCTCTATGGTCCCGCTATGGCCGCTTCCTG
CAGCCGGTGGATGACACGCAGCACCTCACTGTGGCCACGCTGGAGGAGAGACCTTTTGTCATTGTAGAGC
CTGCAGACCCCATCAGCGGCACTTGCATCAGAGACTCGGTTCCCTGCCGGAGCCAGCTCAACCGTACCCA
CAGCCCTCCGCCTGACGCTCCCCGCCCGGAGAAGAGATGCTGCAAGGGTTTCTGCATTGACATTTTGAAG
AGGCTGGCGCACACCATCGGTTTCAGCTATGACCTCTACCTGGTTACCAACGGCAAGCATGGCAAGAAGA
TCGATGGCGTCTGGAATGGCATGATTGGTGAGGTGTTCTATCAGCGTGCGGACATGGCCATCGGCTCCCT
CACCATCAATGAGGAGCGGTCAGAGATCGTGGACTTCTCCGTCCCTTTTGTAGAGACAGGCATCAGCGTC
ATGGTGGCACGCAGCAATGGCACTGTGTCCCCCTCTGCCTTCCTCGAGCCCTACAGCCCCGCTGTGTGGG
TGATGATGTTCGTCATGTGCCTCACCGTGGTCGCCGTCACAGTTTTCATCTTTGAGTACCTCAGTCCTGT
GGGCTATAACCGAAGCCTGGCCACGGGCAAACGCCCCGGAGGCTCTACCTTCACCATTGGGAAATCCATC
TGGCTGCTGTGGGCCCTGGTGTTCAACAACTCCGTGCCAGTGGAGAATCCTCGGGGCACCACCAGCAAGA
TCATGGTGCTGGTGTGGGCCTTCTTTGCCGTCATCTTTCTTGCCAGCTATACAGCCAATCTGGCTGCCTT
CATGATCCAGGAGGAGTACGTGGACACCGTGTCTGGGCTCAGCGACCGGAAGTTCCAGCGGCCCCAGGAG
CAATACCCACCCCTGAAGTTTGGAACGGTGCCCAATGGGTCCACGGAGAAGAATATCCGAAGCAACTACC
CTGATATGCACAGCTACATGGTGCGATACAACCAGCCAAGAGTGGAGGAGGCGCTCACTCAGCTCAAGGC
AGGGAAACTGGACGCCTTCATCTATGACGCAGCAGTGCTCAACTACATGGCCCGAAAGGATGAGGGCTGC
AAGCTGGTCACCATCGGCTCAGGCAAGGTCTTCGCCACCACTGGTTATGGCATCGCCCTACACAAGGGCT
CCCGCTGGAAGAGGCCCATCGACCTGGCGCTGCTGCAGTTCCTGGGGGACGATGAGATTGAGATGCTGGA
GCGGCTGTGGCTTTCAGGGATCTGCCACAACGACAAAATCGAGGTGATGAGCAGCAAGCTGGATATCGAC
AACATGGCAGGTGTCTTCTACATGCTCCTCGTGGCCATGGGCCTCTCCTTGCTGGTCTTCGCCTGGGAAC
ACCTTGTGTACTGGCGACTGCGGCACTGTCTGGGGCCCACCCACCGCATGGATTTCCTACTGGCCTTCTC
CAGGGGTATGTACAGCTGCTGCAGCGCTGAGGCTGCTCCGCCGCCGGCCAAACCCCCGCCACCGCCGCAG
CCGCTGCCCAGTCCGGCGTATCCCGCCGCTCGCCCACCCCCTGGCCCCGCACCCTTCGTGCCCCGAGAGC
GCGCAGCCGCCGACCGCTGGCGCCGGGCCAAGGGCACAGGGCCCCCGGGGGGCGCAGCGCTAGCCGACGG
CTTCCACCGATACTACGGCCCCATCGAGCCGCAGGGGCTGGGCCTCGGCGAGGCGCGCGCGGCACCGAGA
GGCGCAGCCGGACGCCCACTGTCCCCACCCACCACACAGCCCCCACAGAAGCCACCACCTTCCTACTTCG
CCATCGTGCGCGAGCAAGAGCCGGCCGAGCCCCCCGCCGGCGCCTTCCCGGGCTTCCCATCTCCGCCCGC
TCCGCCTGCCGCCGCAGCCGCCGCCGTCGGGCCGCCACTGTGCCGCCTGGCTTTCGAGGACGAGAGCCCG
CCCGCGCCCTCGCGCTGGCCGCGTTCTGACCCCGAGAGCCAGCCGCTGTTGGGTGGGGGCGCGGGCGGCC
CGAGCGCTGGGGCCCCGACCGCACCACCGCCGCGCCGCGCCGCGCCACCACCGTGCGCCTACCTGGACCT
CGAGCCTTCGCCTTCGGACTCCGAGGATTCGGAGAGCCTGGGCGGAGCGTCGCTCGGTGGCCTGGAGCCC
TGGTGGTTCGCCGACTTCCCCTACCCGTATGCGGAGCGCCTCGGGCCGCCGCCCGGCCGCTACTGGTCGG
TTGACAAGCTCGGGGGCTGGCGCGCTGGTAGCTGGGACTACCTGCCCCCGCGCGGCGGCCCCGCATGGCA
CTGCCGCCACTGCGCCAGCCTGGAGCTGCTACCGCCACCACGCCATCTCAGCTGCTCGCACGACGGCCTA
GACGGTGGCTGGTGGGCGCCTCCGCCTCCACCCTGGGCTGCGGGGCCACCGCCCCGGCGCCGGGCGCGCT
GTGGTTGTCCGCGACCGCACCCGCACCGCCCCCGGGCTTCGCACCGTGCGCCCGCCGCCGCACCGCACCA
CCACCGACACAGGCGCGCGGCGGGTGGCTGGGACCTCCCGCCGCCCGCGCCCACCTCGCGTTCGCTGGAG
GACCTGAGCTCCTGCCCACGGGCGGCCCCCACGCGCAGGCTCACCGGGCCCTCGCGCCACGCGCGCCGCT
GTCCGCACGCTGCGCATTGGGGGCCGCCCCTGCCCACCGCATCTCACCGGAGACACCGGGGCGGGGACCT
GGGCACACGCAGGGGCTCTGCGCACTTCTCCAGCCTGGAGTCCGAGGTATGA


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-RsrII     
ACCN NM_008172
Insert Size 3972 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_008172.2, NP_032198.2
RefSeq Size 4383 bp
RefSeq ORF 3972 bp
Locus ID 14814
UniProt ID Q03391
Gene Summary Component of NMDA receptor complexes that function as heterotetrameric, ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+) (PubMed:1385220). Sensitivity to glutamate and channel kinetics depend on the subunit composition (Probable).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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