Cngb1 (NM_001195413) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Cngb1b; Garp2; Gm1959
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224253 representing NM_001195413 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGGGCTGGGTCCAAAGGGTGCTGCCTCAGCCTCCGGGGACCCCTCAGAAGACCGTGGAGACAGCAG GACCACAGCCAGAAACAGAGTCGAAACCAGAGGCCAACCCACAACCGGAGCCAGAACCTCAGCAGGAACC AGAGCCAGAGCCAGAGCCAGAGCCAGAGCCAGAGCCAGAGCCAGAGCCAGAGCCAGAGCCAGAGCCAGAA CCTGAACCTGAACCAGTACCTGAAGAGGCTCCACCTGAGGTCCAGGCCCTGCCACCAGAGGAGCCAATGG AAGGAGAGGGAGAGGCTGAGGCTGGTCCCAGCCTTCAAGAGACCCAGGTAGCTGACCCTGCTCAGCCCAC CTCCCAGGCCCAGGTTGCTGTTGCCAAGGTGAACAGGCCCAGCTCCTGGATGTTGAGCTGGTTCTGGAGA GGCATGCAGAAGGTCGTGCCACAGCCTGTCTGCAGCAATGGGGGCCAGAACCTGGCTGCCGGAGAGCGAG ACCCAGATCAGGGTGGAGCACAGATCCCAGAACCTTGTGGCACTGGGGACCCAGGATCTGCAGAAGCTTC CGGGACTCAGGACACTGAGCCCAGCCTTTGGCTGCTCAGGTGGCTTGAGCAGAATCTGGAAAAAGTGCTG CCCCAGCCCCCTCCGCCTTCCCTGGCCTGGAAAGTTGAACCTGAGGCTGCTGTCTTGGATCCAGATCCTC CAGGAACCCCTATGCAGATGGAGCCCACAGAGAGCCCCTCCCAGCCTAATCCTGGACCCCTGGAGCCTGA GGAAGAGCCAGCTGCAGAGCCCCAGCCTGGCTTCCAATCCTCTTCCCTGCCACCACCCGGGGACCCCGTC AGGCTGATTGAGTGGCTCCTACACAGACTGGAGATGGCCCTGCCTCAGCCCGTGCTCCATGGGAAGGCTG CAGAGCAAGAGCCTGGCTGCCCTGGGATGTGTGACGTACAGACCATCAGCATCCTCCCCGTGGAACAGGT GGAACATGATCTTGTCCTGGAGGAGGTGGACTCTTGCTGGGAGGATGCTCAGCAGGAAGATGGCGCCAGC CCGCAGGAGACAGAGGTGGCTCCAGCTCATGAAGAAGAGAGTGAGGCCATAGTGGAGATACCCAGGGAGC TGACGAAGATTCAAGAAGAAAGAGAAGATGAGCAGGAAGAGGATGAGGAAGAGAAGGAGGAAGAGAAGAA GAAGGGGGAGGAAGAGAAGGAGAAGGAGGAGGAAGAGAAAGAGAAAGAGAAGGAGAAGGAGGAGGAGAAG GAAGAGGAAGAGAAGGAGGAGGAGGAGGAGGAAGAAAAGGAAGAGGAAGAGGAGGAAAAGGAAGAAGAGG AGAAGGAAGAGGAGGAGAAGGAAGAGGAGGAAGAAGAAGAGGAGGAGGAAGAAGAGCCTATTGTCCTGCT GGATAGCTGTTTGGTGGTGCAGGCTGATGTGGACGAGTGCCAGCTAGAAAGGACACCGTCAGAGCTAGCA TCGATCCAGGAGCTGCCAGAAGAGAAAGAGGAGAAGGAAGAGGAGAAGGAGGAGGAGAAAGAGGAGGAGG AGGAAAAGAAGGAGGAAGAAGTGGAGAAGAAGGAGGAAGGGGAGGCCACAAACTCAACAGTACCAGCCAC AAAGGAGCACCCGGAGCTCCAGGTAGAAGACACGGATGCTGATAGTGGCCCCCTCATCCCAGAGGAGACA CTCCCTCCACCTGAGAGACCACCACCGTCTCCTGTCAAGTCGGACACCCTCACAGTTCCCGGCGCCGCTG CAGCAGGCCACAGGAAGAAGCTACCTTCTCAGGATGATGAGGCCGAAGAGCTCAAGGCCCTGTCACCAGC TGAGTCCCCAGTGGTTGCCTGGTCGGACCCCACCACCCCACAGGAGGCTGATGGCCAGGACCGAGCAGCC TCCACCGCCAGCCAGAACAGTGCCATCATCAATGACCGGCTCCAGGAGCTGGTGAAGATGTTCAAGGAGC GGACGGAGAAGGTGAAGGAGAAGCTCATTGACCCTGACGTCACCTCTGATGAGGAGAGCCCCAAGCCCTC CCCGGCCAAGAAGGCCCCAGAACCAGACCCAGCCCAGAAGCCAGCGGAGGCAGAGGTGGCAGAGGAGGAA CACTACTGCGACATGCTCTGCTGCAAGTTCAAGCGCAGGCCCTTGAAGATGTACCGGTTCCCCCAGAGTA TCGACCCACTGACCAACCTCATGTACATCTTGTGGCTGTTCTTTGTGGTGCTGGCCTGGAACTGGAACTG CTGGCTGATCCCTGTGCGCTGGGCCTTCCCGTACCAGCGGGCAGACAACATCCACTTCTGGCTCCTCATG GATTACTTGTGCGACTTCATCTACCTCCTGGACATCACCGTGTTCCAGATGCGCCTGCAGTTTGTCAAAG GCGGGGACATCATTACAGACAAGAAGGAGATGCGTAATAACTACCTGAAGTCTCGCCGGTTTAAGATGGA CCTGCTCTGCCTCCTGCCCTTGGACTTTCTCTACTTGAAACTTGGCATCAACCCTCTCCTTCGCCTGCCC CGCTGCCTGAAGTACATGGCCTTCTTTGAGTTTAATAACCGCCTGGAAGCCATCCTCAGCAAAGCCTACG TTTACAGAGTCATCAGGACCACGGCCTACCTGCTGTACAGCCTGCACCTCAACTCCTGCCTTTACTACTG GGCATCAGCCTTCCAGGGCATCGGCTCCACTCACTGGGTTTACGACGGAGTGGGGAACAGTTATATTCGA TGCTACTACTGGGCTGTGAAAACCCTCATCACCATCGGAGGACTGCCCGACCCCCAGACGCTCTTTGAGA TTGTCTTCCAGCTGCTGAACTATTTTACCGGGGTCTTTGCTTTCTCTGTGATGATTGGACAGATGAGAGA TGTGGTGGGGGCCGCCACAGCGGGGCAGACCTACTACCGCAGCTGCATGGACAGCACAGTGAAGTACATG AACTTCTACAAGATCCCCAGGTCTGTGCAGAACCGTGTCAAGACCTGGTATGAGTACACCTGGCATTCGC AAGGCATGCTGGATGAGTCAGAGCTGATGGTACAACTTCCGGACAAGATGCGACTGGACCTGGCCATTGA TGTGAACTACAGTATTGTCAGCAAAGTGGCCCTCTTTCAGGGCTGTGACCGGCAGATGATCTTCGACATG CTCAAGCGGCTTCGTTCTGTTGTCTACCTACCCAATGACTATGTGTGCAAGAAGGGGGAGATTGGCCGAG AGATGTACATTATCCAGGCGGGGCAGGTGCAGGTGCTGGGCGGCCCAGATGGGAAGGCTGTACTGGTGAC ACTCAAAGCCGGATCGGTGTTTGGAGAAATAAGCTTGCTGGCTGTCGGGGGTGGCAACCGGCGCACAGCC AACGTGGTGGCCCATGGCTTCACCAACCTCTTCATACTGGACAAGAAGGACTTGAATGAAATTTTAGTGC ATTACCCGGAATCTCAGAAGTTACTTCGGAAGAAGGCCAGGCGCATGCTAAGAAATAACAACAAACCCAA GGAGGAAAAGAGTGTGCTCATCCTGCCCCCGCGTGCGGGCACCCCGAAGCTCTTCAATGCTGCCCTGGCT GCTGCAGGAAAGATGGGCCCCAGGGGAGCCAAGGGCGGCAAGCTCGCCCACCTGAGAGCCAGGCTCAAGG AACTGGCTGCACTGGAGGCAGCCGCGCGACAGCAACAGCTGCTGGAACAGGCCAAGAGCTCGCAAGAAGC TGGGGGAGAGGAGGGCTCTGGGGCCACAGACCAACCTGCACCCCAGGAGCCACCAGAGCCCAAGGACCCT CCGAAGCCACCAGGGCCACCAGAGCCCTCAGCCCAGAGCTCTCCACCTCCAGCCTCAGCAAAGCCCGAGG AAAGCACGGGGGAGGCAGCAGGGCCCCCGGAGCCCTCGGTGAGGATCCGTGTGAGTCCAGGCCCTGATCC CGGGGAGCAGACACTGTCGGTGGAGGTGCTGGAGGAGAAGAAAGAGGGGGCGGAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001195413
Insert Size	3978 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001195413.1, NP_001182342.1
RefSeq Size	6201 bp
RefSeq ORF	3978 bp
Locus ID	333329

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.