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Igf1r (NM_010513) Mouse Untagged Clone
CAT＃: MC224342
Igf1r (untagged) - Mouse insulin-like growth factor I receptor (Igf1r), (10ug)
CNY 9,376.00
货期*
现货
规格
| Cited in 3 publications. |
Product images
经常一起买 (4)
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | A330103N21Rik; CD221; D930020L01; hyft; IGF-1R |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC224342 representing NM_010513
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGTCTGGCTCCGGAGGAGGGTCCCCGACCTCGCTGTGGGGGCTCGTGTTTCTCTCCGCCGCGCTCT CTCTCTGGCCGACGAGTGGAGAAATCTGTGGGCCCGGCATTGACATCCGCAACGACTATCAGCAGCTGAA GCGCCTGGAAAACTGCACGGTGATCGAGGGCTTCCTCCACATCCTGCTCATCTCCAAGGCCGAGGACTAC CGAAGCTACCGCTTCCCCAAGCTCACCGTCATCACTGAGTACTTGCTGCTCTTCCGAGTCGCTGGCCTCG AGAGCCTGGGAGACCTCTTCCCCAACCTCACAGTCATCCGTGGCTGGAAACTCTTCTACAACTACGCACT GGTCATCTTCGAGATGACCAATCTCAAGGATATTGGGCTTTATAATCTGAGGAACATTACTCGGGGGGCC ATCAGGATTGAGAAGAACGCCGACCTCTGTTACCTCTCCACCATAGACTGGTCTCTCATCTTGGATGCGG TGTCCAATAACTACATTGTGGGGAACAAGCCCCCGAAGGAATGTGGGGACCTGTGTCCAGGGACATTGGA GGAGAAGCCCATGTGTGAGAAGACCACCATCAACAATGAGTACAACTACCGCTGCTGGACCACAAATCGC TGCCAGAAAATGTGCCCAAGTGTGTGCGGGAAGCGAGCCTGCACCGAGAACAACGAGTGCTGCCACCCGG AGTGCCTGGGCAGCTGCCACACACCGGACGACAACACAACCTGCGTGGCCTGCAGACACTACTACTACAA AGGCGTGTGTGTGCCTGCCTGCCCGCCTGGCACCTACAGGTTCGAGGGCTGGCGCTGTGTGGATCGCGAT TTCTGCGCCAACATCCCCAACGCTGAGAGCAGTGACTCGGATGGCTTCGTTATCCACGACGATGAGTGCA TGCAGGAGTGTCCCTCAGGCTTCATCCGCAACAGCACCCAGAGCATGTACTGTATCCCCTGCGAAGGCCC CTGCCCCAAAGTCTGCGGCGATGAAGAGAAGAAAACGAAAACCATCGATTCGGTGACTTCTGCTCAAATG CTCCAAGGATGCACCATCCTGAAGGGCAATCTGCTTATTAACATCCGGAGAGGCAATAACATTGCCTCGG AGTTGGAGAACTTCATGGGGCTCATCGAGGTGGTGACCGGCTACGTGAAGATCCGCCATTCTCATGCCTT GGTCTCCTTGTCCTTCCTGAAGAACCTTCGTCTCATCTTAGGAGAGGAGCAGCTGGAAGGGAACTACTCC TTCTATGTCCTAGACAACCAGAACTTGCAGCAGCTGTGGGACTGGAACCACCGGAACCTGACCGTCAGGT CCGGAAAGATGTACTTTGCTTTCAATCCCAAGCTGTGTGTCTCCGAAATTTACCGCATGGAGGAAGTGAC CGGAACCAAGGGACGCCAGAGCAAAGGGGACATAAACACCAGGAACAACGGAGAGCGAGCTTCCTGTGAA AGTGATGTTCTCCGTTTCACCTCCACCACGACCTGGAAGAACCGAATCATCATAACGTGGCACCGGTACC GGCCGCCGGACTACCGGGATCTCATCAGCTTCACAGTTTACTACAAGGAGGCACCATTTAAAAACGTTAC GGAATATGACGGGCAGGATGCCTGTGGCTCCAACAGCTGGAACATGGTGGATGTAGACCTGCCTCCGAAC AAGGAGGGCGAGCCTGGCATTTTACTGCATGGGCTGAAGCCCTGGACCCAGTATGCTGTCTATGTCAAGG CTGTGACCCTCACCATGGTGGAAAACGACCATATCCGTGGGGCCAAAAGTGAAATCTTGTACATTCGCAC CAATGCTTCAGTCCCTTCCATTCCCCTAGATGTCCTCTCAGCATCAAACTCTTCCTCTCAGCTGATTGTG AAGTGGAATCCTCCAACTCTGCCCAATGGTAACTTGAGTTACTACATTGTGAGGTGGCAGCGGCAGCCCC AGGATGGTTACCTGTACCGGCACAACTACTGCTCCAAAGACAAAATACCCATCAGAAAGTACGCCGATGG TACCATCGACGTGGAGGAGGTGACGGAAAATCCCAAGACAGAAGTGTGTGGTGGTGATAAAGGGCCATGC TGCGCTTGCCCTAAAACTGAAGCTGAGAAGCAGGCTGAGAAGGAGGAGGCTGAGTACCGTAAAGTCTTTG AGAATTTCCTTCACAATTCCATCTTTGTGCCCAGGCCCGAAAGGAGGCGGAGAGACGTCATGCAAGTGGC CAACACGACCATGTCCAGCCGAAGCAGGAACACCACGGTAGCTGACACCTACAATATCACAGACCCGGAG GAGTTCGAGACAGAGTACCCTTTCTTTGAGAGCAGAGTGGATAACAAGGAGAGGACTGTCATCTCCAACC TCCGGCCTTTCACTCTGTACCGCATCGATATCCACAGCTGCAACCACGAGGCTGAGAAGCTGGGCTGCAG CGCCTCCAACTTCGTCTTTGCGAGAACCATGCCAGCAGAAGGAGCAGATGATATCCCTGGTCCGGTGACC TGGGAGCCAAGACCCGAAAACTCCATCTTTTTAAAGTGGCCAGAACCCGAGAACCCCAACGGATTGATCC TAATGTATGAAATTAAATACGGGTCGCAAGTCGAGGATCAGCGGGAATGTGTGTCCAGACAGGAGTACAG GAAGTACGGAGGGGCCAAACTCAACCGTCTAAACCCAGGGAACTATACAGCCCGGATTCAGGCTACCTCC CTCTCTGGGAATGGGTCATGGACAGATCCTGTGTTCTTCTATGTCCCCGCCAAAACGACGTATGAGAACT TCATGCATCTGATCATTGCTCTGCCGGTTGCCATCCTGCTGATCGTTGGGGGGCTGGTTATCATGCTGTA TGTCTTCCATAGAAAGAGAAATAACAGCAGGTTGGGCAATGGAGTGCTGTATGCTTCTGTGAACCCCGAG TATTTCAGCGCAGCTGATGTGTACGTGCCTGATGAATGGGAGGTAGCTCGAGAGAAGATCACCATGAACC GGGAGCTCGGACAAGGGTCCTTTGGGATGGTCTATGAAGGAGTGGCCAAGGGTGTGGTCAAGGATGAACC CGAAACCAGAGTGGCCATCAAGACGGTAAACGAGGCTGCAAGTATGCGTGAAAGAATCGAGTTTCTCAAC GAGGCCTCGGTGATGAAGGAGTTCAATTGTCACCATGTGGTCCGGTTGCTGGGTGTGGTATCCCAAGGCC AGCCCACCCTGGTCATCATGGAACTAATGACACGCGGTGATCTCAAAAGTTATCTCCGGTCTCTGAGGCC AGAAGTGGAGCAGAATAATCTAGTCCTCATTCCTCCGAGCTTAAGCAAGATGATCCAGATGGCTGGAGAG ATTGCAGATGGCATGGCCTACCTCAATGCCAACAAGTTCGTCCACAGAGACCTTGCTGCTAGGAACTGCA TGGTAGCCGAAGATTTCACAGTCAAAATTGGAGATTTCGGTATGACACGAGACATCTACGAGACGGACTA CTACCGGAAAGGCGGGAAGGGGTTGCTGCCTGTGCGCTGGATGTCTCCCGAGTCCCTCAAGGATGGTGTC TTCACTACTCATTCTGATGTCTGGTCCTTCGGGGTCGTCCTCTGGGAGATCGCCACGCTGGCTGAGCAGC CCTACCAGGGCTTGTCCAACGAGCAAGTTCTTCGTTTCGTCATGGAGGGTGGCCTTCTGGACAAGCCGGA CAACTGCCCTGATATGCTGTTTGAACTTATGCGCATGTGCTGGCAGTATAACCCCAAGATGCGGCCCTCC TTCCTGGAGATCATCGGCAGCATCAAGGATGAGATGGAGCCCAGCTTCCAGGAGGTCTCCTTCTACTACA GCGAGGAGAACAAGCCTCCCGAGCCAGAGGAGCTGGAGATGGAGCCTGAGAACATGGAGAGCGTCCCACT GGACCCTTCGGCCTCCTCAGCCTCCCTGCCTCTGCCTGAAAGACACTCAGGACACAAGGCTGAGAATGGC CCGGGCCCTGGCGTGCTCGTTCTCCGCGCCAGTTTTGATGAGAGACAGCCTTACGCTCACATGAACGGGG GACGCGCCAACGAGAGGGCCTTGCCTCTGCCCCAGTCCTCGACCTGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_010513 |
| Insert Size | 4110 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_010513.2, NP_034643.2 |
| RefSeq Size | 11978 bp |
| RefSeq ORF | 4110 bp |
| Locus ID | 16001 |
| UniProt ID | Q60751 |
| Gene Summary | Receptor tyrosine kinase which mediates actions of insulin-like growth factor 1 (IGF1). Binds IGF1 with high affinity and IGF2 and insulin (INS) with a lower affinity. The activated IGF1R is involved in cell growth and survival control. IGF1R is crucial for tumor transformation and survival of malignant cell. Ligand binding activates the receptor kinase, leading to receptor autophosphorylation, and tyrosines phosphorylation of multiple substrates, that function as signaling adapter proteins including, the insulin-receptor substrates (IRS1/2), Shc and 14-3-3 proteins. Phosphorylation of IRSs proteins lead to the activation of two main signaling pathways: the PI3K-AKT/PKB pathway and the Ras-MAPK pathway. The result of activating the MAPK pathway is increased cellular proliferation, whereas activating the PI3K pathway inhibits apoptosis and stimulates protein synthesis. Phosphorylated IRS1 can activate the 85 kDa regulatory subunit of PI3K (PIK3R1), leading to activation of several downstream substrates, including protein AKT/PKB. AKT phosphorylation, in turn, enhances protein synthesis through mTOR activation and triggers the antiapoptotic effects of IGFIR through phosphorylation and inactivation of BAD. In parallel to PI3K-driven signaling, recruitment of Grb2/SOS by phosphorylated IRS1 or Shc leads to recruitment of Ras and activation of the ras-MAPK pathway. In addition to these two main signaling pathways IGF1R signals also through the Janus kinase/signal transducer and activator of transcription pathway (JAK/STAT). Phosphorylation of JAK proteins can lead to phosphorylation/activation of signal transducers and activators of transcription (STAT) proteins. In particular activation of STAT3, may be essential for the transforming activity of IGF1R. The JAK/STAT pathway activates gene transcription and may be responsible for the transforming activity. JNK kinases can also be activated by the IGF1R. IGF1 exerts inhibiting activities on JNK activation via phosphorylation and inhibition of MAP3K5/ASK1, which is able to directly associate with the IGF1R (By similarity). When present in a hybrid receptor with INSR, binds IGF1 (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (3)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
FoxK1 and FoxK2 in insulin regulation of cellular and mitochondrial metabolism
,Sakaguchi, M;Cai, W;Wang, CH;Cederquist, CT;Damasio, M;Homan, EP;Batista, T;Ramirez, AK;Gupta, MK;Steger, M;Wewer Albrechtsen, NJ;Singh, SK;Araki, E;Mann, M;Enerbäck, S;Kahn, CR;,
Nat Commun
,PubMed ID 30952843
[IGF1R]
|
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Domain-dependent effects of insulin and IGF-1 receptors on signalling and gene expression
,Cai, W;Sakaguchi, M;Kleinridders, A;Gonzalez-Del Pino, G;Dreyfuss, JM;O'Neill, BT;Ramirez, AK;Pan, H;Winnay, JN;Boucher, J;Eck, MJ;Kahn, CR;,
Nat Commun
,PubMed ID 28345670
[IGF1R]
|
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Insulin and IGF-1 receptors regulate FoxO-mediated signaling in muscle proteostasis
,O'Neill, BT;Lee, KY;Klaus, K;Softic, S;Krumpoch, MT;Fentz, J;Stanford, KI;Robinson, MM;Cai, W;Kleinridders, A;Pereira, RO;Hirshman, MF;Abel, ED;Accili, D;Goodyear, LJ;Nair, KS;Kahn, CR;,
J. Clin. Invest.
,PubMed ID 27525440
[Igf1r]
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Documents
| Product Manuals |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG227409 | Igf1r (tGFP-tagged) - Mouse insulin-like growth factor I receptor (Igf1r), (10ug) |
CNY 10,968.00 |
|
| MR227409 | Igf1r (Myc-DDK-tagged) - Mouse insulin-like growth factor I receptor (Igf1r) |
CNY 9,368.00 |
|
| MR227409L3 | Lenti ORF clone of Igf1r (Myc-DDK-tagged) - Mouse insulin-like growth factor I receptor (Igf1r) |
CNY 11,768.00 |
|
| MR227409L4 | Lenti ORF clone of Igf1r (mGFP-tagged) - Mouse insulin-like growth factor I receptor (Igf1r) |
CNY 11,768.00 |
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