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Pola1 (NM_008892) Mouse Untagged Clone
CAT＃: MC224516
Pola1 (untagged) - Mouse polymerase (DNA directed), alpha 1 (Pola1), (10ug)
CNY 18,810.00
货期*
8周
规格
Product images
经常一起买 (3)
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | AW321876; Pola |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC224516 representing NM_008892
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCCCATGCACGAAGAGGACTGTAAACTGGAGGCAAGCGCTGTGTCAGATTCAGGGAGTTTTGCAG CTTCCCGAGCTCGGCGAGAAAAGAAATCAAAGAAAGGACGTCAAGAAGCTTTAGAGAGACTGAAAAAGGC TAAAGCTGGGGAGAAGTATAAATACGAAGTTGAGGACCTCACAAGTGTTTATGAAGAAGTTGATGAAGAG CAGTATTCGAAACTGGTTCAGGCCCGTCAGGATGATGACTGGATTGTGGATGACGATGGGATAGGCTATG TGGAAGATGGCAGAGAGATTTTTGATGATGACCTTGAGGATGATGCACTTGACACCTGTGGAAAAGGAAG TGATGGCAAAGCACACAGAAAGGACAGGAAGGATGTAAAGAAACCTTCAGTGACAAAACCAAACAATATC AAGGCAATGTTCATTGCTAGTGCCGGGAAGAAAACTACAGATAAAGCAGTAGACTTGTCCAAGGATGATC TGCTAGGTGACATTCTACAGGATCTAAACACTGAGACAGCTCAAATAACTCCACCACCTGTATTGATACC AAAGAAAAAAAGATCTACTGGGGCTTTGCTCAACCCTTTTTCAGTGCACACCCCTAAGGCAATTCCTTCA GGAAAACCTGCTTCCCCTGTCCTCAGGAATGAGCCTCTGTTAACTCCCATTCCTCTTAAACGTGCTGAAC TTGCTGGAGAGCTGGCTCAGCCAGAATGTCCAGAAGATGAGCAGGAGCTAGGGGTAATGGAGTTTGAAGA CGGTGACTTTGATGAGTCCATGGACACAGAGAAGGTAGATGAGAAGCCTGTGACTGCTAAGACTTGGGAC CAAGAGACTGAGCCAGTAGAAAGGGTGGAACATGAGGCAGATCCTGAGAGGGGAACCACATCTTACTTAG AAAATTTTCTGCCAGATGTCTCTTGTTGGGACATTGATCAGGATGATGAAAGCATACCTCAGGAAGTTCA AGTGGATTCCAGTAATCTCCCATTGGTAAAAGGGGCAGATGATGAACAAGTGTTTCAGTTTTATTGGCTG GATGCTTATGAAGATCCATACAACCAACCAGGTGTGGTTTTTCTGTTTGGCAAAGTGTGGATTGAATCAG TCAAAACCCATGTGAGCTGTTGTGTTATGGTGAAAAATATTGAGCGAACACTCTACTTCCTTCCCCGTGA AATGAAATTTGACCTAAACACAGGGAAAGAAACAGCAATTCCAGTTACAATGAAGGATGTTTATGAAGAA TTTGATTCAAAAATTTCAGCAAAATATAAAATAATGAAGTTCAAGTCCAAGATAGTGGAAAAGAACTATG CTTTTGAGATACCTGATGTTCCAGAAAAATCTGAGTACCTGGAAGTTAGATACTCGGCAGAAGTGCCACA GCTTCCACAGAATTTAAAAGGAGAAACATTTTCTCATGTGTTTGGAACTAACACGTCTAGCTTGGAACTG TTCTTGATGAATAGGAAGATCAAAGGACCTTGTTGGCTTGAAGTAAAAAATCCACAACTCTTGAATCAGC CAATCAGTTGGTGTAAATTTGAGGTAATGGCTTTGAAACCAGACCTGGTGAATGTAATTAAGGATGTCAG TCCTCCTCCACTTGTGGTGATGTCTTTCAGCATGAAGACAATGCAGAATGTACAGAACCATCAACATGAG ATTATTGCTATGGCGGCTTTGGTTCATCACAGTTTTGCATTAGATAAAGCACCTCCAGAGCCTCCCTTTC AGACACATTTCTGTGTTGTGTCTAAACCAAAGGACTGTATTTTCCCATGTGATTTCAAAGAAGTGATTTC GAAAAAGAATATGAAGGTTGAGATCGCTGCAACAGAAAGAACCTTGATAGGATTTTTCCTTGCAAAAGTT CACAAAATTGACCCTGACATTCTTGTGGGTCATAATATTTGTAGTTTTGAACTGGAAGTGCTGCTGCAAA GAATTAATGAGTGCAAGGTTCCTTACTGGTCCAAGATAGGTCGGCTGAGGAGATCCAACATGCCAAAACT TGGGAGCCGGAGTGGATTTGGTGAAAGAAATGCTACCTGTGGCCGAATGATATGTGATGTGGAAATTTCA GCAAAAGAATTGATTCATTGTAAAAGCTACCATTTGTCTGAACTTGTTCAGCAGATTCTGAAAACTGAGA GGATTGTAATTCCAACAGAAAATATACGAAACATGTATAGTGAATCTTCTTATCTGTTGTACCTGTTGGA ACATATCTGGAAAGATGCCAGATTCATTTTGCAGATCATGTGTGAGCTAAATGTTCTTCCATTAGCATTG CAGATCACTAACATCGCTGGGAACATTATGTCTAGGACACTGATGGGTGGACGATCTGAGCGAAATGAGT TTTTGTTGCTTCATGCATTTTATGAAAACAACTATATTGTGCCTGACAAGCAGATTTTCAGAAAACCTCA GCAAAAACTGGGAGATGAAGATGAGGAAATTGATGGGGACACCAATAAATACAAGAAAGGGCGTAAGAAA GCAACTTACGCTGGAGGCTTGGTTTTGGACCCCAAAGTTGGTTTTTATGACAAGTTCATTTTGCTCCTGG ATTTCAACAGTTTATATCCTTCCATCATTCAGGAATTTAACATTTGTTTTACAACAGTGCAAAGAGTTAC TTCAGAGGTGCAGAAAGCTACAGAGGATGAGGAACAAGAACAGATCCCTGAGTTGCCAGATCCAAACTTA GAGATGGGCATTTTGCCCAGAGAGATTCGGAAACTGGTAGAGCGGAGAAAACAAGTCAAACAGCTAATGA AACAGCAAGATTTAAATCCAGACCTTGTTCTTCAGTATGACATCCGACAGAAGGCTTTGAAGCTCACAGC AAACAGTATGTATGGTTGCCTGGGATTTTCGTACAGCAGATTTTATGCTAAACCACTGGCTGCCTTGGTG ACATACAAAGGAAGGGAGATTTTGATGCATACGAAAGACATGGTACAGAAGATGAATCTTGAAGTTATTT ATGGAGACACAGATTCAATTATGATAAATACCAATAGCACCAACCTGGAAGAAGTATTTAAGTTGGGAAA CAAGGTAAAAAGTGAAGTGAATAAATTGTACAAACTGCTTGAAATAGACATTGATGCTGTTTTCAAGTCT CTGCTGTTGCTGAAGAAAAAGAAATATGCTGCTCTAGTTGTTGAACCAACATCAGATGGGAATTACATTA CCAAGCAGGAGCTGAAAGGATTAGATATAGTTAGAAGAGACTGGTGTGATCTTGCCAAGGACACTGGAAA CTTTGTCATTGGCCAGATTCTTTCTGATCAAAGCCGGGACACCATTGTGGAAAACATTCAGAAGAGGTTA ATAGAAATTGGAGAAAATGTGCTAAATGGCAGTGTCCCAGTGAGCCAGTTTGAAATTAACAAGGCATTGA CAAAGGATCCCCAGGATTACCCTGATAGAAAAAGCTTACCTCATGTACATGTTGCACTGTGGATAAATTC TCAAGGAGGCAGGAAGGTGAAAGCTGGTGATACGGTGTCCTATGTTATTTGTCAGGATGGGTCAAACCTC ACTGCAACTCAGAGGGCCTATGCCCCAGAACAATTGCAGAAACTTGACAATTTAGCCATCGACACCCAAT ACTACTTGGCCCAGCAGATCCACCCTGTTGTGGCTCGGATCTGTGAGCCAATAGATGGAATTGATGCTGT ACTCATTGCATTATGGCTAGGACTTGACTCCACTCAATTTAGAGTTCATCAGTATCACAAAGATGAAGAG AATGATGCCCTCCTTGGTGGTCCAGCACAGCTAACTGATGAAGAGAAATACAAGGATTGTGAAAAATTCA AATGCCTTTGTCCTTCATGTGGAACTGAAAATATTTATGATAATGTCTTTGAAGGCTCGGGACTGGATAT GGAACCCAGTTTGTATCGTTGCAGTAATGTTGATTGTAAGGTTTCACCGTTGACCTTCATGGTACAGCTG AGCAACAAATTGATCATGGACATTAGACGGTGCATTAAAAAGTACTATGATGGCTGGTTGATATGTGAAG AGCCAACTTGTTGCAGTCGCCTTCGACGTCTTCCCCTTCATTTCTCCCGAAATGGGCCTCTGTGTCCAGT CTGCATGAAAGCTGTGCTTAGACCAGAGTATTCTGACAAGTCCTTGTACACCCAGCTGTGCTTTTACCGG TACATTTTTGATGCGGACTGTGCACTGGAAAAACTTACTGAACATGAGAAAGATAAATTGAAGAAGCAGT TTTTCCCTCTCCGAGTTCTTCAGGACTACAGAAAAGTCAAGAACATAGCAGAGCAGTTCTTGTCCTGGAG TGGCTACTCTGAAGTGAACCTCAGCAAGCTCTTCGCCAATTACGCTGGGAAGTCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_008892 |
| Insert Size | 4398 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_008892.2, NP_032918.1 |
| RefSeq Size | 5350 bp |
| RefSeq ORF | 4398 bp |
| Locus ID | 18968 |
| UniProt ID | P33609 |
| Gene Summary | Plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes. In the cytosol, responsible for a substantial proportion of the physiological concentration of cytosolic RNA:DNA hybrids, which are necessary to prevent spontaneous activation of type I interferon responses.[UniProtKB/Swiss-Prot Function] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG226939 | Pola1 (tGFP-tagged) - Mouse polymerase (DNA directed) alpha 1 (Pola1), (10ug) |
CNY 16,060.00 |
|
| MR226939 | Pola1 (Myc-DDK-tagged) - Mouse polymerase (DNA directed), alpha 1 (Pola1) |
CNY 14,630.00 |
|
| MR226939L3 | Lenti ORF clone of Pola1 (Myc-DDK-tagged) - Mouse polymerase (DNA directed), alpha 1 (Pola1) |
CNY 16,530.00 |
|
| MR226939L4 | Lenti ORF clone of Pola1 (mGFP-tagged) - Mouse polymerase (DNA directed), alpha 1 (Pola1) |
CNY 16,530.00 |
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