Grin2b (NM_008171) Mouse Untagged Clone

CAT#: MC224542

Grin2b (untagged) - Mouse glutamate receptor, ionotropic, NMDA2B (epsilon 2) (Grin2b), (10ug)



  "NM_008171" in other vectors (4)

CNY 10,144.00


货期*
现货

规格
    • 10 ug

Cited in 1 publication.

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms AW490526; GluN2B; Nmdar2b; NR2B
Vector pCMV6-Entry2
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC224542 representing NM_008171
Red=Cloning site Blue=ORF

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGCCCAGCGCAGAGTGCTGTTCTCCCAAGTTCTGGTTGGTGTTGGCCGTCTTGGCCGTATCGGGCA
GCAAAGCTCGTTCCCAAAAGAGCGCCCCCAGCATCGGCATCGCTGTCATCCTCGTGGGCACTTCCGACGA
AGTGGCCATAAAAGATGCCCACGAGAAAGATGACTTCCATCATCTCTCAGTAGTTCCCCGGGTGGAGCTG
GTAGCCATGAACGAGACTGACCCAAAGAGCATAATCACCCGCATCTGCGATCTTATGTCTGACCGGAAGA
TCCAGGGGGTGGTGTTCGCGGATGACACGGACCAGGAAGCCATCGCCCAGATCCTCGATTTCATTTCTGC
TCAGACTCTCACCCCCATCCTGGGCATCCATGGGGGCTCATCTATGATAATGGCAGATAAGGATGAGTCC
TCCATGTTCTTCCAGTTTGGCCCATCCATTGAACAGCAAGCTTCTGTCATGCTCAACATCATGGAAGAAT
ACGACTGGTACATCTTCTCCATCGTCACCACCTACTTCCCCGGCTACCAGGACTTCGTGAACAAGATCCG
CAGCACTATTGAGAACAGCTTTGTGGGCTGGGAGCTCGAGGAAGTCCTCCTGCTAGACATGTCTCTAGAC
GATGGCGACTCTAAGATCCAGAATCAGCTGAAGAAGCTGCAGAGCCCCATCATTCTCCTCTACTGCACAA
AGGAAGAAGCCACCTACATCTTCGAAGTAGCTAACTCAGTTGGGCTGACTGGCTACGGCTACACATGGAT
CGTGCCGAGTCTGGTGGCGGGGGATACGGACACGGTGCCTTCAGAGTTCCCCACGGGGCTCATCTCTGTG
TCATATGACGAATGGGACTATGGCCTTCCTGCCAGAGTGAGAGATGGGATTGCCATCATCACCACTGCTG
CCTCGGACATGCTGTCCGAACACAGTTTCATCCCTGAGCCCAAGAGCAGTTGCTACAACACCCACGAGAA
GAGGATCTACCAGTCTAACATGCTGAATAGGTATCTGATCAACGTCACTTTTGAAGGGAGAAACCTGTCC
TTCAGTGAAGATGGCTACCAGATGCATCCGAAGCTGGTGATAATCCTTCTGAACAAGGAGAGGAAGTGGG
AGAGGGTGGGAAAATGGAAAGACAAGTCCCTGCAGATGAAATACTATGTGTGGCCTCGAATGTGTCCTGA
GACTGAAGAACAGGAAGATGACCATCTGAGCATCGTTACCTTGGAGGAGGCACCGTTTGTCATTGTGGAA
AGTGTGGACCCTCTCAGTGGGACCTGCATGCGGAATACAGTCCCGTGCCAGAAGCGCATCATCTCTGAGA
ATAAAACAGATGAGGAACCAGGCTACATCAAAAAATGCTGCAAGGGGTTTTGTATTGATATCCTTAAGAA
AATTTCTAAGTCTGTGAAGTTCACCTATGACCTTTACCTGGTGACCAATGGCAAGCATGGAAAGAAAATC
AACGGGACCTGGAACGGCATGATTGGTGAGGTGGTCATGAAGAGGGCCTATATGGCAGTGGGATCACTAA
CTATCAATGAAGAACGGTCAGAGGTGGTTGACTTCTCTGTGCCCTTCATAGAGACTGGCATCAGTGTCAT
GGTATCACGCAGCAATGGGACTGTGTCACCTTCTGCCTTCTTAGAGCCATTCAGTGCTGACGTGTGGGTG
ATGATGTTTGTGATGCTGCTCATTGTCTCTGCTGTAGCTGTCTTTGTCTTTGAATACTTCAGCCCTGTGG
GTTACAACCGGTGCCTAGCTGATGGCAGAGAGCCAGGCGGCCCATCTTTCACCATCGGCAAAGCGATTTG
GTTACTCTGGGGTCTGGTGTTTAACAACTCCGTACCTGTGCAGAACCCAAAGGGGACCACCTCCAAGATC
ATGGTGTCAGTGTGGGCCTTCTTTGCTGTCATTTTCCTGGCCAGCTACACTGCCAACTTAGCCGCCTTCA
TGATCCAAGAGGAGTATGTGGACCAGGTTTCCGGCCTGAGTGACAAGAAGTTCCAGAGACCTAATGACTT
CTCACCCCCTTTCCGCTTTGGGACTGTGCCCAATGGCAGCACAGAGAGGAATATCCGTAATAACTATGCA
GAAATGCATGCCTACATGGGAAAGTTCAACCAAAGGGGTGTAGATGATGCCTTGCTCTCCCTGAAAACAG
GGAAACTTGATGCATTCATCTACGATGCAGCCGTGCTCAACTACATGGCTGGAAGAGACGAAGGCTGCAA
GCTGGTGACCATTGGCAGTGGCAAGGTCTTTGCTTCTACGGGCTATGGCATTGCTATCCAAAAAGACTCT
GGTTGGAAACGCCAGGTGGACCTTGCTATCCTGCAGCTGTTTGGAGATGGGGAGATGGAAGAACTGGAAG
CTCTCTGGCTCACTGGCATTTGCCACAATGAGAAGAATGAGGTTATGAGCAGCCAGCTGGACATTGACAA
CATGGCGGGCGTCTTCTATATGTTGGGGGCAGCCATGGCTCTCAGCCTCATCACCTTCATCTGTGAACAT
CTCTTCTATTGGCAGTTCCGACATTGCTTCATGGGTGTCTGTTCTGGCAAGCCTGGCATGGTCTTCTCCA
TCAGCAGAGGTATCTACAGCTGTATCCACGGAGTAGCTATAGAGGAGCGCCAATCCGTGATGAACTCCCC
CACTGCCACCATGAACAACACACACTCCAATATCCTACGCTTGCTCCGAACGGCCAAAAACATGGCCAAC
CTGTCTGGAGTCAACGGCTCCCCCCAGAGTGCCCTGGACTTCATCCGCCGTGAGTCCTCTGTCTATGACA
TCTCTGAGCATCGCCGCAGCTTCACGCATTCAGACTGCAAGTCGTACAATAACCCACCCTGTGAGGAAAA
CCTGTTCAGTGACTACATTAGTGAGGTAGAGAGAACATTTGGCAACCTGCAGCTGAAGGACAGCAATGTG
TACCAAGACCACTATCACCATCACCACCGGCCCCACAGCATCGGCAGCACCAGCTCCATTGATGGGCTCT
ATGACTGTGACAACCCACCCTTTACCACCCAGCCCAGGTCAATCAGCAAGAAACCCCTGGACATTGGCCT
GCCCTCCTCCAAACACAGCCAGCTCAGCGACCTGTACGGCAAGTTCTCTTTCAAGAGTGACCGCTACAGT
GGCCATGATGACTTGATTCGATCAGATGTCTCAGACATCTCCACGCATACTGTCACCTATGGCAACATCG
AGGGCAACGCAGCCAAGAGGAGGAAGCAGCAATATAAGGACAGTCTAAAGAAGCGGCCAGCCTCGGCCAA
ATCTAGGAGGGAGTTTGATGAAATCGAGCTGGCCTACCGTCGCCGACCACCCCGCTCCCCAGACCACAAG
CGCTACTTCAGGGACAAAGAAGGGCTCCGAGACTTCTACCTGGACCAGTTCCGAACAAAGGAGAACTCGC
CTCACTGGGAGCACGTGGACTTGACTGACATTTACAAAGAACGTAGTGATGACTTCAAGCGAGATTCGGT
CAGTGGAGGCGGGCCCTGTACCAACAGGTCTCACCTTAAACACGGAACAGGCGATAAGCACGGAGTGGTA
GGCGGGGTGCCTGCTCCTTGGGAGAAGAACCTGACCAATGTGGATTGGGAGGATAGGTCTGGGGGCAACT
TCTGCCGCAGCTGTCCCTCCAAGCTGCACAATTACTCCTCTACGGTGGCAGGGCAAAACTCGGGCCGGCA
GGCCTGCATCAGGTGTGAGGCCTGCAAGAAGGCTGGCAACCTGTATGACATCAGCGAGGACAACTCCCTG
CAGGAACTGGACCAGCCTGCTGCCCCTGTGGCTGTGTCATCCAACGCCTCCACCACCAAGTATCCTCAAA
GCCCGACTAATTCCAAGGCCCAGAAGAAGAATCGGAACAAACTGCGCCGGCAGCACTCCTACGACACCTT
CGTGGACCTGCAGAAGGAGGAGGCCGCCTTGGCCCCACGCAGCGTGAGCCTGAAAGACAAGGGCCGATTC
ATGGATGGGAGCCCCTACGCCCATATGTTTGAGATGCCAGCTGGTGAGAGCTCCTTTGCCAACAAGTCCT
CAGTGACCACTGCCGGACACCATCACAACAATCCCGGCAGCGGCTACATGCTCAGCAAGTCGCTCTACCC
TGACCGGGTCACGCAAAACCCTTTCATCCCCACTTTTGGGGATGATCAGTGCTTGCTTCACGGCAGCAAA
TCCTACTTCTTCAGGCAGCCCACGGTGGCAGGGGCGTCGAAAACAAGGCCGGACTTCCGGGCCCTTGTCA
CCAATAAGCCAGTGGTGTCAGCCCTTCATGGGGCTGTGCCAGGTCGTTTCCAGAAGGACATTTGTATAGG
GAACCAGTCCAACCCCTGTGTGCCTAACAACAAAAACCCCAGGGCTTTCAATGGCTCCAGCAATGGACAT
GTTTATGAGAAACTTTCTAGTATTGAGTCTGATGTCTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI     
ACCN NM_008171
Insert Size 4449 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_008171.3, NP_032197.3
RefSeq Size 7515 bp
RefSeq ORF 4449 bp
Locus ID 14812
UniProt ID Q01097
Gene Summary Component of NMDA receptor complexes that function as heterotetrameric, ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+) (PubMed:1377365, PubMed:26912815). Sensitivity to glutamate and channel kinetics depend on the subunit composition (PubMed:1377365). In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death (PubMed:20141836). Contributes to neural pattern formation in the developing brain (PubMed:8789948). Plays a role in long-term depression (LTD) of hippocampus membrane currents and in synaptic plasticity (PubMed:8789948).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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