Ercc6 (NM_001081221) Mouse Untagged Clone

CAT#: MC224549

Ercc6 (untagged) - Mouse excision repair cross-complementing rodent repair deficiency, complementation group 6 (Ercc6), (10ug)



  "NM_001081221" in other vectors (4)

CNY 19,000.00


货期*
8周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 4732403I04; C130058G22Rik; CSB
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC224549 representing NM_001081221
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTCCACGAGGAAGTTCCCAACTCCACTCACCCTCAGGAGCAAGACTGTTTACCAAGTCAGCATGCCA
ATGCTTACAAAGACATGCCAGTTGGGCAGGAGAATGGTGGGGTCTCAGAGGCCGGGGAGTGCCTCTCCTC
TACTTCCTGTGAGTATGGGCCATCCACATCTGCTGAAGCTTGTGTGTTGGCAGCCACAAGGCGTGGGCCG
ACCCTGCTACACATTGATCGGCATCAGATCCCTGCAGTTGAGCCCAGTGCTCAGGCCCTGGAGCTGCAGG
GCCTGGGTGTGGACGTCTATGACCAGGCTGTCCTGGAACAAGGCGTGCTTCAGCAGGTGGACAGTGCGAT
GCATGAGGCCAGCTGTGTGGCCCAGCTTGCTGATGCGGAGAAGGAGTATCAGTCAGTCCTGGATGACCTC
ATGTCATGTACAACATCCCTGAGGCAAATCAATAAAATTATAGAGCAACTTAGCCCTCAAGCTGCCAGCA
ACAGAGACATCAACAGGAAACTAGATTCCGTGAAGCGACAGAAGTATAACAAGGAACAACAGCTAAAGAA
GATAACTGCAAAACAGAAACGTCTCCAGGCCATTCTCGGAGGGGCTGGGGTCCAAGTGGAACTGGATCAT
GCCAGCCTAGAGGAGGACGATGCAGAGCCAGGGCCATCATGTCTTGGCAGTATGCTCATGCCTGCCCAGG
AGACCGCCTGGGAGGAGCTTATCCGCACTGGCCAGATGACACCGTTTGGTACCCCAGCCCCTCAGAAACA
GGAGAAAAAGCCCAGAAAAATAATGCTGAATGAAGCATCAGGCTTTGAAAAATATTTGGCAGAGCAAGCA
CAACTTTCGTTTGAAAGGAAGAAACAAGCTGCTACTAAAAGAACAGCTAAAAAAGCTATAGTCATATCCG
AGTCAAGCCGAGCAGCAATTGAAACCAAGGCAGACCAGAGAAGCCAAGTGCTCTCTCAGACAGACAAGCG
CCTGAAGAAGCACAGCAGGAAACTCCAGAGGAGGGCGCTGCAGTTCCAGGGCAAAGTGGGATTGCCGAGC
GGGAAGAAACCTTTGGAGCCTGAGGTGAGGCCAGAGGCCGAGGGAGACACTGAGGGTGAGGAGTCTGGGT
CCTCTCCCACCGATGGGGAGGAGGAGGAAGAACAAGAGGAGGAGGAAGGGGTAGCCAGCCTGTCTTCTGA
TGACGTTAGCTATGAGCTGAAGCCTCTTCGGAAGAGGCAGAAATACCAGAAGAAAGTCCCAGTTCAGGAG
ATCGACGATGACTTCTTTCCAAGTTCTGAGGAGGAAGATGAAGCTATGGAAGGAAGAGGAGGAGGCCGAA
AGGTAGCCAGGCGCCAAGACGATGGAGATGAGGATTATTATAAGCAGCGGTTAAGGAGGTGGAACAGGCT
GAGGCTGCAGGACAAAGAGAAACGCCTGAAGCTTGAAGATGATTCTGAGGAGAGCGATGCCGAATTTGAC
GAAGGTTTCAAAGTGCCGGGTTTTCTGTTCAAAAAGCTCTTTAAGTATCAGCAGACAGGTGTTAGGTGGC
TGTGGGAATTGCACTGCCAGCAGGCAGGAGGAATTCTGGGAGACGAAATGGGATTGGGCAAGACCATCCA
GATAATTGCCTTCTTGGCAGGTCTGAGCTACAGCAAGATCAGGACTCGCGGTTCAAATTACAGGTTCGAG
GGACTAGGCCCCACTATAATTGTCTGCCCAACGACAGTGATGCATCAGTGGGTGAAGGAATTCCACACGT
GGTGGCCTCCATTCAGAGTGGCAGTTCTGCATGAAACAGGTTCCTACACCCACAAAAAGGAGAGGCTGAT
TCGAGATATTGTTTATTGTCATGGAGTTTTGATAACTTCATATTCATACATCCGACTGATGCAAGATGAC
ATTAGCAGACATGACTGGCACTACGTCATCTTAGATGAAGGACACAAAATTCGAAATCCAAATGCTGCAG
TCACCCTTGCTTGCAAACAGTTCCGGACACCTCATCGGATTATCTTGTCTGGCTCACCGATGCAGAATAA
CCTCCGAGAGCTGTGGTCACTCTTCGACTTCATCTTCCCAGGAAAGCTGGGCACATTGCCTGTGTTTATG
GAGCAGTTCTCTGTCCCCATCACCATGGGAGGCTATTCCAATGCTTCCCCAGTACAGGTTAAAACTGCTT
ATAAGTGTGCATGTGTCTTAAGAGACACGATAAACCCGTACTTACTGCGGAGAATGAAATCAGATGTCAA
GATGAGCCTTTCGTTGCCAGATAAAAATGAGCAGGTCTTATTTTGCCGTCTAACTGATGAGCAGCATAAA
GTCTACCAGAATTTCATTGATTCTAAAGCAGTCTACAGGATTCTCAATGGAGAAAATCAGATTTTCTCTG
GACTTGTAGCGCTGAGGAAGATCTGCAACCACCCTGACCTCTTTTCTGGCGGCCCCAAGAACGCCAGTGG
TCCTCCAGAGGATGAGCTGGAAGAAGAGCAGTTTGGACACTGGAGACGCTCTGGGAAAATGATTGTTGTT
GAGTCTTTGCTGAAAATATGGCACAGGCAGGGTCAGCGGGTGCTGCTGTTTTCCCAGTCCAGGCAGATGC
TACACATACTGGAAGTGTTCCTGAGAGCCCATAAGTATTCCTATCTCAAGATGGATGGGACCACCACCAT
AGCGTCAAGACAGCCGCTGATTACAAAATACAATGAGGACACGTCCATCTTTGTCTTTCTTCTGACCACA
CGGGTGGGTGGCTTAGGAGTGAACCTGACCGGTGCCAACAGAGTCATCATCTACGACCCAGACTGGAATC
CAAGCACCGACACACAGGCCCGGGAGCGAGCATGGAGGATAGGTCAGAAGAAGCAGGTGACTGTCTACAG
GCTTCTGACGGCTGGCACCATTGAGGAAAAGATCTACCACCGACAAATCTTCAAGCAATTTTTGACAAAC
AGAGTGCTAAAAGACCCAAAACAAAGGCGCTTTTTCAAATCCAACGATCTCTATGAGCTGTTCACTCTGA
CCAGTCCTGACGCCTCCCAGGGCACTGAGACAAGTGCTATTTTTGCAGGAACGGGCTCCAGTATTCAGAC
ACCCAAATGCCAGTTAAAAAAAAGGACTTCCACAGTCCTGGGAACAGATCCCAAATGCAAGAAGCCCCCT
GTTTCTGACACACCTGCAAATGCTGCCACCCTGATTGGAGAAAAACCTAAGGCGGCGGGAGCTACAGGAA
GAAGTGTCACTTCTGGAGAAAGTGGTCCTTTCAAAGGCGACCATGACACAAATGGGAACAGAGCTAGCAG
TGTTGCTTTTGGAGAAGAGACAGATGCAGGGTCCACACTGGAGCATCTCTCAGTGATGAGTGGAGATGGG
AAGCATTCAGATTCTCCAACAGTTGACCACACATCCAGGCCACCAGTGGAGGCAAGCACCAGTGAGAAGC
AGGGTTCTTCCTATGCAGGGGCGCGCTGCCAGGCTCAGACAGAACCTGTTCCCATGAGTGAACAAATGGA
AGGTCAGTTTTCTAAGTACAAATCCAAAAGGAAGCATGATGCATCTGAGGAAGAGACCACAGAGAAACGT
CCACAGCCCAAGCAAAAGGCCAAGAACTCCAAGCATTGCAGAGATGCTAAGTTTGAGGGAACTCGAGTCC
CGCACCTGGTGAAGAAAAGACGGTACCGCCAGCAAACCAGTGAGCAGGAGGGCGGAGCCAAGGACCGGAG
CAGCGATGATTATGTGTTGGAAAAGCTTTTCAAAAAATCAGTGGGCGTGCACAGCGTCGTGAGGCATGAT
GCCATCATAGATGGGTCCAGTCCAGATTATGTGCTGGTGGAAGCAGAAGCCAACCGAGTGGCTCAGGACG
CCTTGAAAGCTCTGAGGCTCTCTCGTCAGCAATGCCTGGGGGCAGCATCTGGTGTTCCCACCTGGACTGG
CCACAGGGGGATTTCTGGTGCACCAACAGGAGTAAAGAATAGGTTCGGTCAGAAAAGGGACTCCAGCCTC
CCTGTGCAGCATCCTTCATCACTAACAGAAAAGACTCAGAACAACATGAAAAAGGAGGGAAAAGCTCACA
CCCCTGAGCACTTCAGTGGCAAAGAAGATGGAGCATCTGTGTCTGGAGCGCCCAGTTCCTCCTCCCTCTT
GGCCAGAATGCGAGCAAGAAACCACATGATTTTGCCGGAGCGCTTAGAGAGTGACAGTGAACATCTTGCT
GAGGCTGCTGCCGTGCCTCCCTGCGGCACCGAACACGACGACCTTCTGGTGGACATGAGGAACTTCATTG
CTTTCCAGGCCCAGGTGGATGGCCAGGCCAGCACTCAGGAAATCCTGCAGGAGTTTGAGTCCAAGCTGTC
CGTGGCACAGTCCTGTGTCTTCCGAGAACTGCTGAGAAATCTGTGCAATTTCCATAGAACTCCTGGTGGG
GAAGGGATTTGGAAGCTGAAGCCAGAATACTGCTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001081221
Insert Size 4446 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001081221.1, NP_001074690.1
RefSeq Size 8422 bp
RefSeq ORF 4446 bp
Locus ID 319955
UniProt ID F8VPZ5
Gene Summary Essential factor involved in transcription-coupled nucleotide excision repair which allows RNA polymerase II-blocking lesions to be rapidly removed from the transcribed strand of active genes (By similarity). Upon DNA-binding, it locally modifies DNA conformation by wrapping the DNA around itself, thereby modifying the interface between stalled RNA polymerase II and DNA (By similarity). It is required for transcription-coupled repair complex formation. It recruits the CSA complex (DCX(ERCC8) complex), nucleotide excision repair proteins and EP300 to the sites of RNA polymerase II-blocking lesions (By similarity). Plays an important role in regulating the choice of the DNA double-strand breaks (DSBs) repair pathway and G2/M checkpoint activation; DNA-dependent ATPase activity is essential for this function (By similarity). Regulates the DNA repair pathway choice by inhibiting non-homologous end joining (NHEJ), thereby promoting the homologous recombination (HR)-mediated repair of DSBs during the S/G2 phases of the cell cycle (By similarity). Mediates the activation of the ATM- and CHEK2-dependent DNA damage responses thus preventing the premature exit from the G2/M checkpoint (By similarity). Acts as a chromatin remodeler at DSBs; DNA-dependent ATPase-dependent activity is essential for this function (By similarity). Remodels chromatin by evicting histones from chromatin flanking DSBs, limiting RIF1 accumulation at DSBs thereby promoting BRCA1-mediated HR (By similarity). Required for stable recruitment of ELOA and CUL5 to DNA damage sites (By similarity). Involved in UV-induced translocation of ERCC8 to the nuclear matrix (By similarity). Essential for neuronal differentiation and neuritogenesis; regulates transcription and chromatin remodeling activities required during neurogenesis (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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