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Eif2ak4 (NM_001177806) Mouse Untagged Clone
CAT＃: MC224577
Eif2ak4 (untagged) - Mouse eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant 2, (10ug)
CNY 10,520.00
货期*
现货
规格
Product images
经常一起买 (3)
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 2610011M03; GCN2; MGCN2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC224577 representing NM_001177806
Red=Cloning site Blue=ORF TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCACCTACATACCCAGATGTGTGATGATATTTGAACTGGCACACCATGTGCAGTCGTTTCTCAGCG AGCATAACAAGCCCCCACCAAAGTCTTTCCATGAAGAAATGCTGGAAAGGCAGGCTCAGGAGAAGCAGCA GAGGTTGCTGGAGGCCAGGCGGAAGGAGGAGCAGGAGCAACGTGAAATCCTCCACGAGATTCAGAGAAGG AAAGAGGAGATCAAGGAAGAGAAGAAAAGGAAGGAAATGGCTAAGCAGGAACGTTTGGAAATCACTAGCT TGACAAACCAGGACTATGCCTCTAAGAGAGACCCAGCAGGGCACAGGGCAGCTGCCATCCTCCATGGAGG CTCTCCTGACTTTGTAGGAAATGGTAAAGCCCGGACATACTCCTCAGGAAGGTCCAGGCGAGAACGTCAG TATTCTGTGTGTAGTGGTGAACCCTCTCCTGGCTCGTGTGACATCCTCCATTTCTCTGTGGGTAGCCCTG ATCAGCTCATGGTGCACAAAGGGAGATGTGTTGGCAGTGATGAGCAGCTGGGAAAGGTGGTATACAATGC TTTGGAAACAGCCACGGGCAGCTTTGTCTTGCTGCATGAGTGGGTCCTTCAATGGCAGAAGATGGGCCCT TGCCTTACCAGTCAAGAGAAAGAGAAGATTGACAAGTGCAAAAGGCAGATTCAAGGAGCAGAAACAGAAT TCAGCTCCCTAGTGAAACTGAGCCATCCAAATATCGTCCGCTACTTTGCGATGAACTCCAGAGAGGAGGA GGACTCCATTGTGATCGACATTCTGGCAGAGCACGTCAGTGGCATCTCTCTGGCTACACACCTGAGCCAC TCGGGCCCAGTCCCCGCCCACCAGCTGCGCAAGTACACAGCCCAGCTCCTGGCAGGCCTGGATTACCTAC ACAGCAACTCCGTGGTGCACAAGGTTCTGAGCGCGTCCAGTGTCTTGGTAGATGCCGAGGGCACTGTCAA GATAACAGACTACAGCATCTCTAAGCGTCTGGCAGACATTTGCAAGGAGGATGTATTTGAGCAAGCTCGA GTTCGTTTTAGTGACAGTGCCCTGCCTTATAAAACAGGAAAGAAAGGGGATGTGTGGCGGCTCGGCCTCC TGCTGTTGTCTCTCAGCCAAGGACAGGAGTGTGGGGAGTATCCGGTGACCATCCCCAGTGACCTGCCAGC TGACTTCCAAGACTTCCTGAAGAAGTGTGTCTGCCTGGACGACAAGGAAAGATGGAGCCCTCAGCAGCTG CTGAAACACAGCTTTATAAACCCTCAACCAAAGCTGCCTTTGGTGGAGCAGAGTCCTGAAGACTCTGGGG GACAAGACTACATTGAGACCGTCATTCCCAGCAACCAGCTGCCCAGCGCTGCGTTCTTCAGTGAGACACA GAAACAGTTCTCCCGGTACTTCATTGAGTTTGAAGAACTACAACTCCTAGGGAAAGGAGCTTTTGGAGCT GTCATCAAGGTGCAAAACAAGCTTGATGGCTGCTGCTATGCTGTGAAGCGCATCCCTATCAACCCTGCAA GCAGACACTTCCGCCGGATCAAGGGCGAGGTGACACTGCTATCGCGCCTGCACCATGAGAACATTGTGCG CTACTACAACGCCTGGATCGAGCGGCATGAGCGCCCAGCGGTGCCAGGGACACCGCCCCCAGACTGCACA CCCCAGGCCCAGGACAGCCCAGCCACCTGCGGGAAAACATCAGGCGACACTGAAGAACTGGGCAGCGTGG AGGCTGCAGCACCGCCACCCATCCTCAGCAGCTCGGTGGAGTGGAGCACATCTGCAGAGCGTTCTACCAG CACCCGCTTCCCAGTCACGGGCCAGGATTCCAGCAGCGATGAGGAGGACGAGGACGAGCGCGATGGCGTC TTCTCCCAGTCCTTTCTACCTGCTTCAGATTCTGACAGTGACATCATCTTTGACAATGAAGATGAAAACA GTAAAAGTCAAAATCAGGATGAAGACTGCAATCAAAAGGACGGTAGCCATGAGATTGAGCCTTCAGTGAC AGCTGAGGCTGTGCACTACCTTTATATCCAGATGGAGTACTGCGAGAAGAGCACGCTGCGGGACACCATT GACCAGGGGCTGTTCCGAGACACCAGCCGGCTCTGGAGGCTTTTCCGAGAGATTCTGGATGGATTAGCTT ATATCCATGAGAAAGGAATGATTCATCGCGACTTGAAGCCTGTCAATATTATTTTGGATTCTGATGACCA TGTGAAAATAGGTGACTTTGGCCTGGCAACAGACCATCTGGCCTTCACTGCTGAAGGTAAACAGGACGAC CAGGCAGGTGACGGAGTGATTAAGTCAGACCCTTCAGGCCATTTGACTGGCATGGTTGGTACTGCTCTGT ATGTAAGCCCTGAGGTCCAAGGAAGCACCAAGTCTGCATACAACCAGAAAGTGGATCTCTTCAGCCTGGG AATTATCTTCTTTGAGATGTCTTATCACCCGATGGTGACTGCCTCAGAAAGAATTTTCGTTCTCAACCAA CTTCGAGATCCCACATCGCCCAAGTTTCCAGACGACTTCGATGATGGAGAACATACAAAGCAGAAATCTG TCATCTCCTGGCTGTTGAACCATGATCCAGCTAAACGGCCCACGGCCATGGAACTGCTCAAGAGTGAGCT GCTCCCCCCGCCGCAGATGGAGGAGTCCGAGCTGCATGAAGTGCTGCACCACACGCTGGCCAACATAGAT GGGAAGGCCTACCGCACCATGATGAGTCAGATCTTCTGCCAGCACATCTCCCCCGCTATAGACTACACCT ACGACAGTGACATCCTGAAGGGCAACTTCTTGATTCGTACAGCCAAGATCCAGCAGCTTGTATGTGAAAC CATCGTCCGCGTCTTTAAAAGGCATGGCGCTGTCCAGCTGTGCACCCCACTGCTGCTTCCCCGAAACAGG CAAATATATGAGCACAACGAAGCGGCTTTGTTCATGGATCACAGCGGCATGCTGGTGATGCTTCCGTTCG ACCTGCGGGTCCCTTTTGCAAGATATGTGGCAAGAAATAACATACTGAATTTAAAACGATACTGCATAGA GCGGGTGTTCAGACCTCGGAAGTTAGACCGATTTCATCCCAAAGAACTCCTGGAATGTGCATTTGATATC GTCACTTCTACCACCAACAGCTCTCTGCCCACTGCCGAAACCATCTACACCATCTATGAAATAATCCAGG AGTTTCCTGCACTTCAGGAAAGGAATTACAGCATTTACTTGAACCATACGATGCTTCTGAAGGCAATACT CCTACACTGTGGGATCCCAGAGGACAAGCTCAGCCAAGTCTACGTCATTCTGTATGATGCTGTGACAGAG AAGCTGACTAGGAGAGAAGTGGAAGCTAAATTCTGTAACCTGTCCTTGTCTTCTAATAGCCTGTGTAGAC TCTACAAGTTCATTGAGCAGAAGGGGGATTTGCAAGACTTAACGCCAACCATCAACTCACTAATAAAACA GAAAACAGGCGTTGCTCAGTTGGTGAAGTATAGCTTAAAAGACCTCGAGGACGTCGTCGGGCTGCTGAAG AAACTTGGCGTGAAGTTACAGGTCTCCATCAACCTGGGCTTGGTCTACAAGGTGCAGCAGCACACTGGCA TCATCTTCCAGTTCCTGGCATTCAGCAAACGCAGGCAAAGGGTTGTGCCTGAGATCCTTGCCGCCGGTGG CAGATACGACCTGCTGATTCCCAAGTTCAGAGGCCCACAGACTGTGGGGCCAGTCCCCACTGCTGTCGGT GTCAGCATAGCCATAGACAAGATATTTGCTGCTGTCCTCAACATGGAAGAGCCTGTTACAGTGAGCTCCT GTGACCTCCTGGTTGTCAGTGTTGGCCAGATGTCCATGTCCAGGGCCATCAACCTAACCCAGAAACTGTG GACAGCGGGCATCACTGCAGAAATCATGTATGACTGGTCCCAGTCCCAGGAAGAGTTACAAGAGTACTGC AGACACCATGAAATCACCTATGTGGCTCTGGTCTCCGATAAAGAAGGAAGCCATGTCAAGGTCAAGTCCT TTGAGAAGGAGAGGCAAACAGAAAAGCGTGTATTGGAATCGGATCTTGTGGATCACGTTATGCAGAAACT AAGAACCAAAGTTGGTGACGAAAGAAATTTCAGAGACGCTTCTGATAATCTTGCCGTACAAACTCTGAAG GGGTCGTTTTCTAATGCTTCAGGTTTGTTTGAAATCCATGGAACTACAGTAGTCCCCAACGTGATTGTTC TAGCACCAGAAAAGCTGTCAGCCAGCACCAGGAGGCGACATGAGATTCAGGTGCAAACCCGACTTCAGAC TACACTTGCCAACTTACATCAGAAAAGCAGTGAAATTGAAATTTTGGCTGTGGACCTACCCAAGGAAACA ATCTTACAGTTTCTATCATTAGAGTGGGATGCTGATGAACAGGCATTTAACACAACTGTGAAGCAGCTGC TGTCACGCCTGCCAAAGCAAAGATACCTCAAACTCGTCTGCGATGAAATTTATAACATCAAAGTTGAAAA GAAGGTGTCAGTGCTGTTCCTGTACAGCTACAGAGATGACTACTACAGAATCCTATTTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001177806 |
| Insert Size | 4611 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC072637, AAH72637 |
| RefSeq Size | 4951 bp |
| RefSeq ORF | 4611 bp |
| Locus ID | 27103 |
| UniProt ID | Q9QZ05 |
| Gene Summary | Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (eIF-2-alpha/EIF2S1) on 'Ser-52' in response to low amino acid availability (PubMed:10504407, PubMed:10655230, PubMed:12176355, PubMed:12215525, PubMed:15213227, PubMed:16054071, PubMed:16176978, PubMed:16121183, PubMed:15774759, PubMed:16601681, PubMed:26102367). Plays a role as an activator of the integrated stress response (ISR) required for adapatation to amino acid starvation. Converts phosphorylated eIF-2-alpha/EIF2S1 either to a competitive inhibitor of the translation initiation factor eIF-2B, leading to a global protein synthesis repression, and thus to a reduced overall utilization of amino acids, or to a translational initiation activation of specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion (PubMed:10655230, PubMed:11106749, PubMed:12176355, PubMed:15213227, PubMed:16176978, PubMed:26102367). Binds uncharged tRNAs (By similarity). Involved in cell cycle arrest by promoting cyclin D1 mRNA translation repression after the unfolded protein response pathway (UPR) activation or cell cycle inhibitor CDKN1A/p21 mRNA translation activation in response to amino acid deprivation (PubMed:16176978, PubMed:26102367). Plays a role in the consolidation of synaptic plasticity, learning as well as formation of long-term memory (PubMed:16121183). Plays a role in neurite outgrowth inhibition (PubMed:23447528). Plays a role in feeding behavior to maintain amino acid homeostasis; contributes to the innate aversion toward diets of imbalanced amino acid composition (PubMed:16054071, PubMed:15774759). Plays a proapoptotic role in response to glucose deprivation (PubMed:20660158). Promotes global cellular protein synthesis repression in response to UV irradiation independently of the stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK) and p38 MAPK signaling pathways (PubMed:12176355).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence and lacks an alternate internal exon in the 5' end compared to variant 1, that causes a frameshift. The resulting isoform (2) has a shorter and distinct N-terminus compared to isoform 1. |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR212025 | Eif2ak4 (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant 2 |
CNY 10,520.00 |
|
| MR212025L3 | Lenti ORF clone of Eif2ak4 (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant 2 |
CNY 13,970.00 |
|
| MR212025L4 | Lenti ORF clone of Eif2ak4 (mGFP-tagged) - Mouse eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant 2 |
CNY 13,970.00 |
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