Tiam2 (NM_001122998) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	3000002F19Rik; mKIAA2016; STEF
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224598 representing NM_001122998 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGAACTCCGAGAGTCAATATACCTTCCAAGGATCCAAGAATCATAGTAATACTGTCACTGGTGCTA AGCAAAAGCCTTGCTCTCTGAAAATACGCAGCGTTCATGCAAAAGACGAGAAGTCCTTACATGGTTGGAC TCATGGGAGCAGCGGTGCAGGCTACAAGTCCAGGTCCCTAGCCCGAAGCTGCCTTTCTCACTTTAAGAAT CACCAGCCTTACGCCACCAGACTCAGTGGACCCACATGTAAAGTCTCAAAGGGCACCACCTACTCTAAGC ACAGAGCAAATACCCCGGGAAATGATTTCCAGGGCAACAGTGGTGCTTTCTTACCTGAGAATGGCTTCCA CTATGTTGACCGCGAGTCAGAGGAAAGCCATATCACCTCCAATGGGCACCTTCTCACCTGCTATGGGAGA AAGGAAAGCCTCGCCTCCACTCCTCCAGGCGAGGACCACAGGAGCCCCAGGGTGCTCATCAAGACCCTGG GAAAGCTAGACGGGTGTTTAAGAGTCGAGTTCCACAACGGTGGCAACCCCCACAAGGGGACCTCCGAGGA CCCCAGTGGACCTGTACGGCTGCTGAGATACTCCCCTACCTTAGCATCGGAAACCTGCCCGGTGCGGGAA ACCAGGCGGCATTCCGCTGCAGGCTCCCCATCCAGCCAGCGGCCCTCTCCCACTGACTCTCGCCTGCGCT CCAGCAAAGGCAGCTCCCTGAGCTCAGAGTCATCCTGGTATGACTCCCCCTGGGGCAACGCTGGGGAGGT GAGCGAGGTGGAGGGCTCCTTCCTGGCTCCCAGCACTCCAGACCCCAGCCTCCCCAGCAGCTTCCCACCC AGTGACACCAAAAAGCCTTTCAACCAAAGCTCTTCCCTCTCCTCCCTCCGGGAATTGTACAAAGATCCCA ACCTGGGGTGCCGCTCACCTTCCGGCACCTGCCTTTCTTCCAATGAGTACATCAGCTCTCAAGTCAGCCT GAACAACCGAGTCTCCTTTGCGTCTGACATGGATGTGCCCTCCAGGGTGGATCACAGGGATCCCCTGCAC TATAGTTCCTTTACTCTCCCCTGTCGCAAGTCCAAAGCCTTAACTGAAGATGCAGCTAAGAAAGACACCC TCAAAGCCAGAATGCGGCGCTTCAGTGACTGGACAGGAAGCCTCTCCAGGAAGAAGAGGAAACTGCAGGA ACCCAGGTCCATGGAGGGCAGTGAGTACTTTGATAGCCACTCAGATGGACTGAATGCAGAAGGGCAGGTG CCCGCGCAGACATCTTCCTTACTGTGGTCAGGGGGCTCGGCTCAGACCCTGCCTCACAGAAGCGAATCCA CTCACGCTATCAGCGTCGATCCCCTCCGACAGAACATCTATGAGAATTTCATGCGAGAGCTCGAAATGAG CAGGAGCAACACAGAACACGTGGAAACTTCCACAGAGACCATGGAGTCCAGCAGCGAGTCTGTCAGCTCA CTGGAGCAGCTGGATCTGCTCTTTGAGAAGGAGCAGGGAGTGGTCCGGAAAGCCGGGTGGCTCTTCTTCA AACCCCTTGTCACCTTGCAGAAGGAGAGGAAACTGGAGCTGGTGGCTCGGAGGAAGTGGAAACAATACTG GGTGACCCTGAAAGGCTGTACTCTGCTGTTTTATGAGACCTATGGAAAGAATTCCACAGAGCAGAATAGT GCCCCACGGTGTGCCCTCTTTGCAGAGGACAGCATCGTGCAGTCTGTCCCAGAGCATCCCAAGAAGGAAC ACGTGTTCTGCCTGAGTAACTCCTGTGGGGACGTCTACCTATTCCAGGCCACTAGCCAGACAGATCTGGA AAACTGGGTCACAGCCATCCACTCGGCGTGCGCATCCCTCTTTGCAAAGAAGCACGGAAAGGAGGACACG GTGCGACTGCTAAAGAGCCAGACCAGAAGCCTGCTTCAGAAGATAGACATGGATAGCAAGATGAAGAAGA TGGCAGAGTTGCAGCTGTCTGTGGTGAGCGACCCCAAGAACAGGAAGGCCATCGAGAATCAGATCCGGCA ATGGGAGCAGAATCTGGAAAAATTCCACATGGACCTGTTCCGCATGCGCTGCTATTTGGCGAGCTTACAA GGTGGGGAGTTACCAAATCCCAAGAGTCTCCTTGCTGCCACCAGCCGCCCCTCCAAGCTGGCTCTTGGCA GGCTGGGCGTCCTGTCTGTTTCGTCTTTCCATGCTCTGGTGTGTTCCAGAGATGATTCCACTCTCAGGAA AAGAACACTTTCCCTTACCCAGAGAGGAAAAAGCAAGAAAGGCATATTTTCTTCATTGAAAGGTCTGGAC ACCCTAGCAAGAAAGGGTAGGGAGAAGAGAGCTTCCATAACTCAGATGTTTGATTCTAGCCACAGCCATG GATTTCTTGGAACTCAGCTACCTCAAAAGTCCACTAACTCCAACAAGGCCCATGACCTGCATCTGTATGG CTCCGCAGTAGACAGCGCGCTGCGAGACAGCATGTGGGAAGTCCAGACTTATGTCCACTTCCAGGATAAC GAAGGAGTTACTGTGACCATCAAGCCAGAGCACAGGGTGGAAGATGTTCTGGCTTTGGTGTGCAAGATGA GACAGTTGGAACCCACTCACTATGGTCTTCAGCTCCGAAAGGTGGTCGATAAAAGTGTGGAGTGGTGTGT GCCCGCGCTGTATGAATACATGCAAGAGCAGGTTTATGATGAAATCGAAGTTTTCCCACTCAGTGTGTAT GACGTGCAGCTAACCAAGACTGGGGACATGACTGACTTTGGGTTTGCAGTCACAGTTCAGGTGGACGAAC ACCAGCATCTCAACCGGATATTTATCAGTGATGTTCTCCCTGACAGCCTGGCATACGGAGGAGGGCTGAG AAAGGGCAACGAAATCACAAGCTTAAATGGGGAACCAGTGTCTGACCTTGACATCCAGCAGATGGAGGCT TTGTTTTCTGAGAAGAGCGTTGGCCTCACTCTAGTCGCCCGGCCTGTGACCACAAGACGAACCCTGTGTG CTTCCTGGTCAGACAGTGACCTGTTCTCCAGGGACCAGAAAAGTCTGCCGCCCTCTCCCAACCAGTCCCA GCTGCTGGAGGAATTCCTGGATAACTTTAGAAAAACCGCCACGAGTGATTTCAGCAATGTCCCTGAGATC ACAACTGGCTTGAAGAGGAGCCAGACAGAAGGCACCCTGGATCAAGTGCCCCACAGGGAAAAGATGGAGC AGACATTCCTGAGCGCTGACCAGATTGCGGAGCTCTGCAGGGACTTGAACAACACCCACACCAACAGTAT GGAAGCACCAACAGAGAGCCATGACCCACCTCCCAGGCCTCTGGCTCGTCACCTCTCAGATGCAGATCGC CTCCGGAAAGTCATCCAGGAGCTTGTAGACACAGAGAAGTCTTACGTGAAGGATCTGAGCTGCCTCTTTG AACTATACTTGGAGCCACTTCAGAATGAGACCTTTCTTACCCAAGATGAGATGGAGTCACTTTTTGGGAG CCTGCCAGAGATGCTGGAGTTTCAAAAGGTGTTCCTGGAGACGTTGGAGGATGCGATCTCCGCTTCCTCG GACTTTAGTGTCCTGGAAACCCCCTCACAGTTTCGAAAATTGCTGTTCTCCCTTGGAGGTTCTTTCCTCT ACTATGCGGATCACTTTAAGCTATACAGTGGGTTCTGTGCCAACCACATTAAAGTACAGAGGGTTCTAGA GCGAGCTAAAACGGACAAGGCCTTCAAGGCTTTTCTGGATGCCCGAAATCCCACCAAGCAGCACTCCTCC ACGCTGGAGTCCTATCTCATCAAGCCTGTTCAGAGAGTGCTCAAGTATCCTCTGCTTCTCAAGGAGCTAG TGTCACTGACTGACCATGAGAGTGAAGAACACTATCACCTGACAGAAGCACTAAAGGCCATGGAAAAAGT CGCCAGTCACATCAATGAGATGCAGAAGATCTACGAGGATTACGGGATGGTGTTTGACCAGCTGGTGGCA GAGCAGAGTGGCACAGAGAAGGAAGTGACAGAGCTGTCCATGGGGGAACTTCTGATGCACTCTACAGTTT CCTGGTTGAATCCGTTCCTGTCTCTAGGAAAAGCCAGGAAGGACATTGAGCTCACAGTATTTGTTTTTAA GAGAGCTGTCATACTGGTTTATAAAGAAAACTGCAAGCTGAAAAAGAAACTGCCCTCGAATTCCCGGCCT GCTCACAACTCTGCTGACTTGGATCCATTTAAATTCCGCTGGTTGATTCCCATATCTGCGCTTCAAGTTA GACTGGGGAACACGGCAGGGACTGAAAATAATTCCACGTGGGAGCTGATTCATACCAAGTCGGAAATTGA AGGACGGCCAGAAACCATCTTTCAACTGTGCTGCAGTGACAGCGAGAACAAAACCAGCATTGTTAAGGTG ATTCGTTCTATTCTGAGAGAGAACTTCCGGCGCCACATAAAGTGTGAGCTGCCACTGGAGAAGACGTGTA AGGACCGGCTAGTACCTCTTAAGAACCGAGTTCCTGTTTCAGCCAAATTAGCCTCGTCCAGGTCGTTGAA GGGCCTCAGAACATCCTCCAGCAGCGAGTGGCCCAGCGAGCCCAGCAAGGGCAACTCACTGGACTCAGAT GAGTGCAGCCTGAGCAGTGGCACCCAGAGTAGCGGCTGCCCCGTAGCCGAGAGCAGGCGAGACTCTAAGA GCACCGAGCTGGAGAAAGACGCTCAGGAGGGCCTGGCGGAGTTTCCAGATGGTCTTATCAAAGAAAGCGA CATTCTGAGTGATGAAGATGAGGACTTCCACCACCCTCTGAAACAGGGTAGCCCTACTAAGGACATTGAG ATTCAGTTCCAGAGACTGAAAATCTCTGAGGAATCCGACGTGCACCCAGTTGGGCAGCAGCCTCTCACAG AGTCAGGTGAACAGCCCAAGCTGGTCAGGGGCCATTTTTGCCCCATTAAACGGAAAGCAAACAGCACCAA GAGGGGCAGAGGAACTTTGCTCAAGGCGCAGACTCGTCACCAGTCCCTGGACAGCCACCCAGAAACTGCC AGCATTGATCTAAACTTGGTCCTGGAGAGAGAATTCAGTGTCCAGAGCTTAACTTCAGTCGTCAATGAGG AGGGTTTTTATGAAACACAGAGCCATGGCAAATCATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001122998
Insert Size	5148 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001122998.1, NP_001116470.1
RefSeq Size	6144 bp
RefSeq ORF	5148 bp
Locus ID	24001
UniProt ID	Q6ZPF3
Gene Summary	Modulates the activity of RHO-like proteins and connects extracellular signals to cytoskeletal activities. Acts as a GDP-dissociation stimulator protein that stimulates the GDP-GTP exchange activity of RHO-like GTPases and activates them. Activates specifically RAC1, but not CDC42 and RHOA. Mediates extracellular laminin signals to activate Rac1, contributing to neurite growth. Involved in lamellipodial formation and advancement of the growth cone of embryonic hippocampal neurons. Promotes migration of neurons in the cerebral cortex. When overexpressed, induces membrane ruffling accompanied by the accumulation of actin filaments along the altered plasma membrane.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) has an alternate 5' UTR exon, compared to variant 1. Variants 1 and 2 encode the same isoform (1).

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