Nrxn1 (NM_020252) Mouse Untagged Clone

CAT#: MC224615

Nrxn1 (untagged) - Mouse neurexin I (Nrxn1), transcript variant 1, (10ug)



  "NM_020252" in other vectors (4)

CNY 19,290.00


货期*
8周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 1700062G21Rik; 9330127H16Rik; A230068P09Rik; mKIAA0578
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC224615 representing NM_020252
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGACGGCGCTGGTCCAGCGCGGGGGCTGCTGTCTCCTCTGCCTGTCGCTGCTGCTGCTGGGCTGCT
GGGCAGAGCTGGGCAGCGGGCTGGAGTTCCCGGGCGCCGAGGGCCAGTGGACGCGCTTCCCCAAGTGGAA
CGCGTGCTGCGAGAGCGAGATGAGCTTCCAGCTGAAGACGCGCAGTGCCCGCGGCCTCGTGCTCTACTTC
GACGACGAGGGCTTCTGCGACTTCCTCGAGCTCATCCTGACGCGCGGCGGCCGCCTGCAGCTCAGCTTCT
CCATCTTCTGCGCCGAGCCCGCCACGCTGTTGGCCGACACGCCGGTCAACGACGGCGCCTGGCACAGCGT
GCGCATCCGCCGCCAGTTCCGCAACACCACGCTCTACATCGACCGCGCCGAGGCCAAGTGGGTGGAGGTC
AAGTCCAAGCGCAGGGACATGACGGTGTTCAGCGGGCTCTTCGTGGGCGGCCTGCCACCCGAACTGCGCG
CCGCGGCCCTCAAGCTCACGCTGGCCTCGGTGCGCGAGCGCGAGCCCTTCAAGGGCTGGATCCGTGACGT
CCGGGTCAACTCGTCACAGGCCCTGCCGGTGGACGGCGGCGAGGTCAAGCTGGACGACGAGCCTCCCAAC
AGCGGTGGCGGGAGCCCATGCGAGGCGGGCGAAGAAGGCGAGGGCGGCGTGTGCCTCAACGGGGGCGTGT
GCTCCGTGGTCGACGACCAGGCCGTATGCGACTGCTCGAGGACCGGCTTCCGTGGCAAGGACTGCAGCCA
AGAAGACAACAATGTGGAAGGTCTGGCACACCTGATGATGGGCGACCAAGGTAAAAGTAAAGGAAAAGAA
GAGTATATTGCCACGTTCAAGGGATCTGAATACTTCTGCTACGACTTATCTCAAAACCCCATTCAAAGCA
GCAGTGATGAAATAACTCTGTCCTTTAAAACTCTTCAAAGGAACGGACTGATGCTTCACACAGGGAAATC
AGCTGATTATGTCAATCTTGCATTGAAAAATGGAGCTGTCTCTCTGGTCATTAATTTGGGATCAGGGGCC
TTTGAAGCACTAGTGGAGCCTGTGAATGGAAAGTTTAATGATAATGCCTGGCATGATGTGAAAGTCACCA
GGAATCTGCGTCAGCACTCAGGCATTGGACACGCTATGGTGACAATATCAGTGGATGGAATTCTTACCAC
AACGGGCTACACGCAAGAAGATTACACCATGCTGGGGTCTGATGACTTTTTCTATGTTGGAGGCAGCCCC
AGCACAGCCGACCTTCCAGGGTCACCAGTCAGTAACAACTTTATGGGCTGTCTCAAAGAGGTTGTATATA
AAAATAATGATGTAAGGCTGGAATTATCGCGACTTGCCAAGCAAGGAGATCCTAAGATGAAGATTCACGG
TGTGGTGGCTTTTAAGTGTGAAAATGTGGCAACCTTAGACCCAATAACTTTTGAGACCCCGGAATCTTTC
ATTTCTTTGCCTAAGTGGAATGCAAAGAAAACAGGCTCAATATCATTTGATTTCCGTACAACGGAGCCAA
ATGGCCTCATCCTGTTTAGCCATGGCAAGCCAAGACATCAGAAGGATGCCAAGCACCCACAGATGATTAA
GGTTGACTTTTTTGCTATTGAGATGCTGGATGGCCACTTGTACCTCCTCTTAGACATGGGATCAGGTACT
ATAAAAATCAAAGCCCTGCAGAAGAAAGTCAATGATGGAGAATGGTATCACGTGGATTTCCAACGGGATG
GACGGTCAGGTACCATTTCTGTCAACACACTACGCACTCCCTACACAGCTCCCGGTGAGAGTGAGATCCT
GGACCTAGATGATGAGTTGTACCTGGGTGGCTTGCCAGAAAATAAGGCTGGCCTAGTCTTCCCTACCGAG
GTGTGGACTGCTCTGCTCAACTACGGCTACGTGGGCTGCATCCGGGACCTATTTATTGATGGCCAGAGCA
AAGACATCCGGCAGATGGCGGAAATTCAGAGCACTGCTGGAGTGAAGCCATCCTGCTCAAAAGAGACAGC
AAAACCGTGCCTTAGCAATCCTTGCAAAAACAATGGCATGTGCAGAGATGGCTGGAACAGATACGTCTGC
GATTGCTCCGGAACAGGCTATCTCGGCAGGTCCTGTGAGAGAGAGGCAACGGTTTTGAGCTACGACGGGA
GCATGTTCATGAAAATCCAGCTTCCGGTGGTGATGCACACTGAGGCTGAGGACGTGTCCTTGCGGTTCCG
ATCCCAGCGTGCATATGGTATCCTGATGGCGACCACTTCTAGAGACTCTGCAGACACCCTTCGATTGGAG
CTAGATGCAGGACGTGTGAAACTCACGGTCAATCTAGATTGTATCAGGATTAACTGTAATTCCAGCAAAG
GTCCTGAGACCCTTTTTGCTGGCTATAACCTCAATGATAATGAATGGCACACAGTGCGTGTGGTTCGGCG
AGGAAAAAGTTTAAAGCTAACCGTGGATGATCAACAAGCCATGACAGGCCAAATGGCAGGTGATCATACA
AGGCTGGAGTTTCATAACATAGAGACAGGCATCATCACCGAACGACGCTACCTTTCTTCTGTCCCCTCCA
ATTTCATTGGGCACCTGCAAAGCCTGACATTTAATGGAATGGCATACATAGACTTGTGCAAAAATGGTGA
TATAGATTACTGTGAACTCAATGCCAGGTTTGGATTCAGGAACATCATCGCAGATCCTGTCACCTTCAAG
ACCAAATCGAGCTATGTTGCCTTAGCTACATTGCAAGCCTACACCTCTATGCATCTATTTTTCCAATTCA
AGACAACATCTCTAGATGGACTAATTCTGTATAACAGTGGGGATGGAAATGACTTTATTGTGGTTGAATT
AGTTAAAGGGTACTTACATTATGTGTTTGACTTGGGAAACGGTGCTAACCTCATCAAAGGGAGCTCAAAC
AAACCACTCAACGACAATCAGTGGCACAATGTGATGATTTCACGGGACACCAGCAATCTCCACACAGTAA
AGATCGACACAAAAATCACAACACAAATCACTGCGGGAGCCAGAAATCTAGACCTCAAGAGTGACTTGTA
TATTGGAGGAGTGGCTAAAGAAACATATAAATCCCTGCCAAAACTGGTCCATGCCAAAGAAGGCTTTCAA
GGTTGCCTGGCATCTGTGGACTTGAATGGCAGACTTCCAGATCTCATCTCGGATGCTCTCTTCTGCAATG
GGCAAATTGAGAGAGGATGCGAAGGGCCCAGCACAACCTGCCAAGAGGATTCATGTTCAAATCAAGGCGT
TTGCTTGCAGCAATGGGATGGCTTCAGCTGTGACTGTAGCATGACTTCCTTCAGTGGCCCACTCTGCAAT
GACCCTGGGACAACATATATCTTTAGCAAAGGTGGTGGACAGATTACATATAAGTGGCCTCCCAATGACC
GCCCCAGTACACGAGCAGACAGGCTGGCCATCGGATTTAGCACTGTCCAGAAGGAAGCCGTGTTGGTGCG
TGTGGACAGTTCCTCAGGACTGGGTGACTACCTGGAGCTGCACATACACCAAGGAAAAATTGGAGTTAAG
TTTAATGTTGGGACAGATGACATCGCCATCGAAGAGTCTAATGCAATCATTAATGATGGGAAATACCATG
TAGTACGTTTCACAAGGAGTGGTGGCAATGCCACGTTACAGGTGGACAGCTGGCCAGTTATCGAACGCTA
CCCTGCAGGAAACAATGATAACGAGCGCCTGGCGATTGCTAGACAGCGAATTCCATATCGACTTGGTCGA
GTAGTTGATGAATGGCTACTCGACAAAGGGCGTCAGCTCACAATCTTCAATAGCCAAGCAACCATAATAA
TTGGCGGGAAAGAGCAGGGCCAGCCCTTCCAGGGCCAGCTCTCTGGTCTTTACTACAATGGCTTGAAAGT
TCTGAATATGGCAGCAGAGAACGATGCCAACATCGCCATAGTGGGGAATGTGAGGCTGGTCGGTGAAGTG
CCTTCCTCTATGACAACTGAGTCGACAGCCACTGCCATGCAGTCTGAGATGTCCACCTCAATCATGGAGA
CCACCACAACCCTGGCTACCAGCACAGCTAGAAGAGGCAAGCCCCCCACAAAGGAACCCATCAGCCAGAC
CACAGATGACATCCTTGTGGCCTCGGCAGAGTGTCCCAGTGACGATGAGGACATTGACCCCTGTGAGCCG
AGCTCAGGTGGGTTAGCCAACCCCACCAGAGTAGGTGGCCGTGAACCATACCCAGGCTCTGCAGAGGTGA
TTCGGGAGTCCAGCAGTACCACTGGCATGGTGGTGGGGATTGTCGCAGCAGCCGCTCTGTGCATCCTCAT
CCTCCTCTATGCCATGTACAAGTACAGGAACCGGGATGAAGGGTCGTACCACGTGGATGAGAGTCGAAAC
TACATCAGTAACTCAGCACAGTCCAATGGGGCTGTGGTCAAGGAGAAGCAACCCAGCAGTGCTAAAAGCG
CCAACAAAAACAAGAAAAACAAGGATAAGGAGTATTACGTCTGA


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-RsrII     
ACCN NM_020252
Insert Size 4524 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_020252.3, NP_064648.3
RefSeq Size 9040 bp
RefSeq ORF 4524 bp
Locus ID 18189
UniProt ID Q9CS84
Gene Summary This gene encodes a single-pass type I membrane protein that belongs to the neurexin family. Neurexins are synaptic transmembrane receptors that bind endogenous ligands that include neuroligins, dystroglycan, and neurexophilins. Neurexin complexes are required for efficient neurotransmission and are involved in synaptogenesis. In vertebrates, alternate promoter usage results in multiple isoform classes, of which the alpha and beta classes are the best characterized. In humans, allelic variants in this gene are associated with Pitt-Hopkins-like syndrome-2, while deletions have been associated with autism and schizophrenia. Mouse knockouts display decreased spontaneous and evoked vesicle release resulting in impaired synaptic transmission. In addition, knockout mice show altered social approach, reduced social investigation, reduced locomotor activity, and in males, increased aggression. Alternative splicing and promoter usage result in multiple transcript variants. [provided by RefSeq, Nov 2016]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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