Topbp1 (NM_176979) Mouse Untagged Clone

CAT#: MC224628

Topbp1 (untagged) - Mouse topoisomerase (DNA) II binding protein 1 (Topbp1), (10ug)



  "NM_176979" in other vectors (4)

CNY 19,380.00


货期*
8周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 1110031N14Rik; 2810429C13Rik; AI256758; D430026L04Rik; mKIAA0259
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC224628 representing NM_176979
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCAGAAATGACCAAGAGCCGTTTCTTGTGAAGTTTTTAAAGTCTTCGGACAATTCAGAATGCTTTT
TTAAAGCTCTTGAGTCAATAAAGGAATTACAATCAGAAGATTATCTTCAGATTATTACAGATGAGGAAGC
ATTGAAGATAAGAGAGAATGACAAATCGCTTTATATCTGTGACCGTTTCAGTGGCACTGTTTTTGATCAC
CTTAAACAGCTTGGCTGCAGGATTGTTGGTCCTCAAGTGGTCACATTTTGTATGCGCCACCAGCAATGTG
TCCCAAGAGCTGAGCATCCAGTCTACAACATGATAATGTCTGACGTAACTGTATCCTGTACAAGCCTGGA
TAAAGACAAACGGGAAGAAGTTCATAAATACGTACAAATGATGGGTGGACGAGTATACAGAGACCTTAAT
GTATCAGTCACTCACCTTATTGCAGGAGAAGTTGGGAGCAAAAAGTACTTAGTTGCTGCTAATCTGAAGA
AGCCTATTTTGCTACCTTCTTGGATAAAAACCCTTTGGGAGAAGTCACAAGAAAAAAAAATAACCAAGTA
CACTGATGTAAACATGGAAGACTTTAAGTGTCCTATTTTTCTTGGTTGCATTATCTGTGTGACTGGCTTA
AATGGAATACACAGAAAGACAGTCCAGCAACTCACGGCTAAGCACGGAGGCCAGTACATGGGACAGTTGA
AGATGAATGAGTGTACACACCTCATTGTGCAAGAACCAAAAGGACAAAAATACGAATGTGCCAGGAGATG
GAATGTGCACTGTGTGACCCTGCAATGGTTTCATGACAGTATTGAGAAAGGCTTTTGTCAGGATGAATCT
ATATACAAGGCTGAAACCAGAGTAGAAGCAAAGATGGTGCCTGATACTTCAACACCCACTGCTCAGAGCA
ATGCTGAAAGTCACACGCTCGCAGATGTCAGCCATATATCCAACATAAATGGCAGCTGTGTAAATGAAAC
AATGTTTGGTTCAACTACTAGCAAACTTGAATGTTCCCTTGAAAACCTAGAAAATCTGGACATCAGTATG
TTTCAAGCTCCTGAAGACTTATTAGATGGCTGTCGGATATATCTTTGTGGTTTTAGTGGCAGAAAGCTGG
ATAAGCTGAGAAGGCTTATTAACAGTGGAGGTGGAGTTCGATTTAATCAACTAAATGAAGATGTAACTCA
TGTAATTGTGGGAGATTATGATGACGACGTGAGGCAGTTCTGGAGCAAATCATCTCACAGGCCTCACGTA
GTGGGAGCAAAGTGGTTGCTGGAGTGTTTTACTAAAGGATATATACTTCCTGAAGAGTCCTATATCCACA
CTAACTACCAGCCAGCTGGAATTGCAGTTTCCGATCAGCCTGGAAACCAGACAGCTGTCTTAGACAAGAG
CGGCTCATTCTCCAAGAGCGCCCTTGTTCCTGCTGAGAGGCTTCAGCAGGCCGACGAGGATCTGCTTGCT
CAGTATGGAAATGACGACTCCACGATGGTTGAAGCTAAGCTATCTGAAGCTTTAGAGCCTGAAGTCGGGC
CTTGCCCTGGTTCTGCTCACCGTGAGCCCTGTGATGATTCCACTCACATTTCTGTGCAAGAGGAAAACAA
GTCGTCTGTCAGTCATTGCATCCTTGATGACTCTACAGTTCGTGAAGAAGGCTTATTTAGCCAGAAGAGT
TTCCTTGTTTTGGGTTTTAGTGTTGAAAATAAATGTAATATTGTAGACATCATAAGAGAGCATGCTGGCA
AGATTGTGTCCCTTCCCAGCAGAATTGTTGCTGACTATGCTGTGGTTCCACTGCTGGGATGTGAGGTAGA
CGTGACGGTGGGAGAAGTTGTTACAAACACGTGGCTGGTTACGTGTATAGACAACCAGACATTAGTTGAT
CCCAAGTCGAATCCCCTCTTCACACCAGTCTCAGTAATGTCGGGAGTGACTCCTTTAGAGGACTGTGTGA
TTTCCTTCAGTCAGTGTGTGGGAGCAGAAAGAGATTCCTTGGTATTCTTAGCAAATCACCTTGGAGCAAG
TGTTCAAGAATTCTTTGTTCGCAAAGCCAATGCAAAGAAAGGCATGCTCGCCAGCACACATCTTATAGTG
AAGGAACCGACTGGTTCCAAATACGAAGCTGCAAAGAAATGGAGTTTGCCGGCAGTTAACATTTCATGGC
TCTTAGAAACTGCGAGAATCGGGAAGAGAGCAGATGAAAACCATTTTCTGGTTGACAACGCACCTAAACA
AGAACAAGTTTTAGAAACTAAGATACCAAATGGAGTGAGTTCAAATCCAGATTTACCAGCACATCCTGAC
GCACACTTGGAAATTCACAGAAAAAAGGCGGTTACACCTTTGGACATGAATCGCTTTCAGAGTAGAGCCT
TCCGTGCTGTCATTTCACAGCAGAGGGGGCAGGACCCCACCTTTCCACCAGTACGGCAGCCCCTTACTAA
GGAGCCCTCTTTACACCTGGACACACCATCAAAGTTTCTGTCCAAGGACAAGCTTTTCAAGCCATCCTTT
GATGTGACGGATGCACTTGCAGCCCTGGAAACCCCAAATGCTGCCAGCCAGAAGCGGAAACTAAGCTCAC
CACTCTCTGAAGTCATCGTCAGAAACCTGACAGTTGCTTTGGCAAATAGTTCCCGAAACACTGACAGTCA
TTCTGCCAGCCCTCAGCTGAAGGGTGCCCATTTGGAGGAGGAAGAGACTCGGAAACCACTTGACAGCGTT
GTGGTGTGTGTGAGTAAGAAGCTCAGTAAGAAGCAGAGTGAGCTCAATGGGGTCGCAGCATCACTGGGAG
CAGAGTACAGGTGGAGTTTCGATGAGACTGTTACTCATTTCATTTACCAGGGTCGGGCAAATGACAGTAA
TCGAGAATATAAATCTGCAAAGGAAAGAGGGGTGCACATCGTGTCCGAGCATTGGCTATTAGAGTGTGCT
CAAGAGTATAAGCATCTTCCTGAATCTCTTTATCCACACACTTACAATCCCAAAATGAGTTTGGACATCA
ACACAGTGCAAGATGGGCGGCTCTGTAACAGCCGGGCACCCTTGGCAGTTTCTGCATCTAAGGATGATGG
GCCAGATCATCTGTCTGTAGAAGGAAATGAGACAAACACTATGGGCACAAATGACAAAGAGTCACCTCTG
TTGAATGGGAGTGGAAGAGATGACTGTAAAGGAGCTCTGACCCAGGCCTTGGAGATGAGAGAGAACTTCC
AGAAGCAGCTGCAAGAGATCATGTCAGCCACCTGCATTGTGAAGACGCCTGCCCAGAAGACTTGCATGTC
AAGAAGCAGCTGTAACAGCGCCTCTTCAACTCCTGACAGCGCTCGCTCTGTGCGGAGCGGACGAAGCCGA
GTCCTGGAGGCACTCAGGCAGTCTCGGCAGGCAGTGCCTGATGTGAACACGGAGCCGTCCCAGAACGAGC
AGATCATTTGGGACGACCCGACAGCAAGGGAAGAGAGAGCAAGGCTTGCCAGCAACTTACAGTGGCCCAG
CGACCCCACACAGCATTCTGAGCTTCAGGTTGAGATAAAAATGCCGGATGACTCTCCCTCTCGAAAGCCT
GTGTACCATTCAGAAATTGCTGAGCAGGCCTCGTGTGTTACTCAAGCCCCAGGGCACCCAGGCTCTGAAG
AACCAGAGCCTCCAGTAGCAGAAAGGCCTTTGATCCCCGAGCCGCAAGCCCCCGCTGTAGCTTCCCCCCT
GGCTAAGCCGCCTGTGGCCCCACAACCTGCAGACAAGATTGAAACACAGGAGGAGACGCACAGAAAAGTT
AAAAAACAGTATGTGTTCCAGATGTCGTCTCTGAATTCTCAAGAGCGGATTGATTACTGTCGCCTGATTA
AAGACTTAGGTGGGTCGGTGATCGAGAAGCAGTGCTCTGACCCCAGCTGCACACACATGGTTGTGGGCTA
CCCACTGCGCAATGAGAAGTACTTAGCTTCTATGGCAGCTGGTAAATGGGTGCTTCATCGCTCCTACCTA
GACGCTTGCAAGACAGCTGGGCGCTTTGTGCAGGAAGAAGACTACGAGTGGGGAAGTAGCTCCATACTTG
ATGCTCTCCCTGACGTCACTGAGCACCAGCAGAAGCTCGCACTGGCGGCAATGAGATGGAGGAAAAGAAT
CCAGCAAAGTCAAGAATCCGGCATTGTTGAGGGAGCATTTAGTGGCTGGAAAGCTATTTTACGTGTTGAT
CGGCCCCGAGAAGCTGGGTTCAAACGCCTTCTTCAGGCAGGAGGAGCAAAGGTGTTATCTGGTCATCCAG
AACCTTTACTTAAAGATGCTACGCATCTCTTTTGTGACTTCAATAAACTGAAACCTGATGACTGCAGAGT
TTTCATCGCAGAGGCTACTGCCCAGAACATGGTCTGCTTGAAAACAGAATATATCGCTGATTATCTCATG
CTGGAATCCCCTCCTTGTGCAGACAATTACCGTGTATCCGAAGCTGCTTTATTCCATAATAAGAAAGGTG
GGCCTGGATTACCACAGAAGAGGAAAACTCCTGCAGAGAATGTAGTCAAACGGCCTCGAGTACACTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_176979
Insert Size 4548 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_176979.5, NP_795953.2
RefSeq Size 5112 bp
RefSeq ORF 4548 bp
Locus ID 235559
UniProt ID Q6ZQF0
Gene Summary Required for DNA replication (By similarity). Plays a role in the rescue of stalled replication forks and checkpoint control (PubMed:14718568). Binds double-stranded DNA breaks and nicks as well as single-stranded DNA (By similarity). Recruits the SWI/SNF chromatin remodeling complex to E2F1-responsive promoters. Down-regulates E2F1 activity and inhibits E2F1-dependent apoptosis during G1/S transition and after DNA damage (By similarity). Induces a large increase in the kinase activity of ATR (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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