Eprs (NM_029735) Mouse Untagged Clone

CAT#: MC224631

Eprs (untagged) - Mouse glutamyl-prolyl-tRNA synthetase (Eprs), (10ug)



  "NM_029735" in other vectors (4)

CNY 19,380.00


货期*
8周

规格
    • 10 ug

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 2410081F06Rik; 3010002K18Rik; C79379; Qprs
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC224631 representing NM_029735
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCGCTGTGCCTCACGGTGAACGCCGGAAACCCTCCGCTGGAAGCTCTGCTGGCGGTGGAACATG
TGAAAGGTGATGTCAGCATTTCTGTGGAAGAAGGGAAAGAGAATCTTCTTCGGGTTTCTGAGACTGTGGC
ATTCACTGACGTGAATTCAATCCTGCGCTACCTGGCTAGAATTGCAACTACGTCTGGGCTGTATGGGACT
AACCTGATGGAGCACACTGAGATTGATCATTGGTTGGAGTTTAGTGCTACAAAGTTGTCTTCGTGTGATA
GGCTGACTTCTGCAATCAACGAGCTTAATCATTGCCTGTCTCTGAGAACATACCTAGTTGGGAACTCCTT
GACCTTAGCAGATCTATGTGTTTGGGCCACCCTAAAAGGAAGTGCTGCATGGCAGGAACACTTGAAACAG
AACAAAACTCTAGTCCACGTTAAACGCTGGTTTGGCTTTCTTGAGGCCCAGCAGGCCTTCCGCTCAGTTG
GCACCAAGTGGGATGTTTCAGGAAACAGAGCTACAGTGGCACCTGACAAAAAACAAGATGTTGGGAAATT
CGTGGAGCTTCCAGGTGCAGAGATGGGAAAGGTGACAGTCAGATTCCCCCCAGAGGCTAGTGGTTACTTA
CACATTGGGCATGCGAAAGCTGCTCTTCTGAACCAGCACTACCAGGTTAACTTTAAAGGGAAACTGATCA
TGAGATTTGATGACACAAACCCAGAAAAGGAAAAGGAAGATTTTGAGAAGGTCATCTTGGAAGATGTTGC
AATGCTGCATATTAAACCAGATCAGTTTACTTACACTTCAGATCATTTTGAAACTATAATGAAGTATGCA
GAGAAATTAATTCAAGAAGGGAAGGCTTATGTGGATGACACTCCTGCAGAACAGATGAAGGCGGAACGTG
AACAGAGGACAGAATCCAAACACAGGAAGAACTCTGTTGAGAAGAATCTACAGATGTGGGAAGAAATGAA
AAAAGGAAGCCAGTTTGGTCAGTCCTGTTGCCTGCGAGCAAAAATTGACATGAGTAGTAACAATGGGTGT
ATGAGAGACCCGACCCTTTACCGATGCAAAATCCAGCCCCACCCAAGGACTGGGAATAAATACAATGTTT
ATCCAACATATGACTTTGCCTGCCCTATAGTGGACAGCATTGAAGGTGTTACTCATGCCTTGAGGACAAC
AGAGTACCACGACCGAGATGAACAATTTTATTGGATCATTGAAGCTTTAGGCATAAGGAAACCATACATC
TGGGAGTACAGTCGCTTAAATCTCAACAACACCGTGCTGTCCAAAAGAAAGCTCACGTGGTTTGTCAATG
AAGGACTGGTAGATGGATGGGATGACCCACGGTTTCCTACAGTTCGTGGAGTTCTGAGAAGAGGGATGAC
AGTTGAGGGGCTGAAGCAGTTCATTGCTGCTCAGGGCTCCTCAAGGTCTGTTGTGAACATGGAATGGGAC
AAAATCTGGGCGTTCAACAAAAAGGTTATTGACCCAGTGGCTCCACGGTACGTTGCATTGCTGAAGAAGG
AAGTCGTCCCAGTGAATGTCCTGGATGCGCAGGAGGAGATGAAGGAAGTAGCCAGACACCCCAAGAATCC
TGATGTTGGTTTGAAGCCTGTGTGGTACAGTCCCAAGGTTTTCATAGAAGGGGCCGATGCAGAGACTTTC
TCAGAGGGTGAGATGGTCACATTTATAAACTGGGGCAACATCAACATTACTAAAATACACAAAAATGCAG
ATGGAAAAATTACATCTCTAGATGCAAAATTGAATTTGGAAAACAAAGACTACAAGAAAACCACCAAGAT
CACTTGGCTTGCTGAGAGCACACACGCTCTCTCGATCCCAGCCGTCTGTGTCACTTATGAGCACTTGATC
ACAAAGCCAGTGCTGGGAAAAGATGAGGACTTCAAGCAGTACATCAACAAAGACAGTAAGCATGAAGAGC
TAATGCTAGGAGATCCTTGCCTTAAAGATCTGAAAAAAGGAGACATCATACAACTGCAGAGGAGAGGGTT
CTTCATATGCGACCAGCCATACGAACCTGTCAGCCCATATAGTTGCAGAGAAGCTCCATGCATCCTGATA
TACATACCTGATGGGCACACAAAGGAAATGCCAACATCCGGATCAAAGGAAAAGACCAAAGTGGAAATCT
CAAAAAAGGAGACAAGCTCTGCTCCTAAAGAAAGACCAGCACCTGCTGTGAGTTCTACTTGTGCTACAGC
TGAGGATTCCTCCGTCCTCTACAGCAGAGTGGCGGTGCAGGGGGATGTGGTTCGTGAATTGAAAGCCAAG
AAAGCGCCAAAGGAAGATATAGACGCAGCTGTGAAGCAGCTTTTGACTTTGAAAGCTGAATATAAAGAGA
AAACTGGGCAGGAGTATAAACCTGGGAACCCCTCTGCTGCAGCAGTACAGACTGTTTCTACAAAGTCATC
ATCAAACACTGTGGAAAGTACATCTTTATACAATAAAGTCGCTGCCCAAGGGGAGGTGGTCCGAAAGCTG
AAAGCGGAAAAGGCTCCTAAGGCTAAAGTAACTGAAGCTGTGGAATGTTTGCTGTCCCTGAAAGCTGAGT
ATAAAGAAAAGACTGGGAAGGACTATGTACCTGGCCAGCCCCCAGCATCGCAGAACTCACATTCAAACCC
TGTCAGTAATGCTCAGCCTGCTGGAGCGGAGAAACCAGAAGCCAAAGTGCTATTTGACAGAGTGGCTTGT
CAAGGAGAAGTAGTTCGAAAACTTAAAGCTGAAAAGGCCTCTAAGGATCAAGTGGATTCGGCGGTACAAG
AACTTCTTCAGCTGAAGGCACAGTACAAGTCGCTGACAGGAATCGAGTATAAGCCTGTGTCTGCTACTGG
GGCAGAAGACAAAGATAAAAAGAAGAAAGAAAAAGAAAATAAATCTGAAAAGCAGAACAAGCCTCAGAAA
CAAAATGATGGCCAAGGGAAAGACTCTTCCAAAAGCCAAGGCAGTGGCCTGTCATCAGGTGGAGCAGGAG
AAGGGCAGGGGCCTAAGAAACAGACCAGATTGGGTCTTGAAGCAAAAAAAGAAGAAAATCTTGCCGAATG
GTACTCTCAAGTCATCACAAAGTCCGAGATGATTGAATACTACGATGTGAGTGGCTGCTATATCCTCCGT
CCCTGGTCGTACTCCATCTGGGAGTCCATTAAGGACTTCTTTGACGCTGAGATCAAGAAGCTCGGTGTTG
AGAACTGCTACTTCCCCATATTTGTGTCTCAGGCTGCGTTAGAGAAAGAGAAGAATCACATCGAAGACTT
TGCTCCTGAGGTTGCTTGGGTTACAAGATCAGGAAAAACAGAGTTGGCAGAGCCAATTGCCATTCGTCCT
ACTAGTGAAACAGTGATGTATCCGGCATATGCTAAATGGGTGCAGTCCCACAGAGACCTGCCCGTCAGGC
TCAACCAGTGGTGCAATGTGGTGCGCTGGGAGTTCAAACACCCACAGCCTTTCCTCCGAACTCGGGAGTT
CCTGTGGCAGGAGGGGCACAGCGCCTTTGCCACCTTTGAGGAAGCAGCTGATGAGGTTTTACAGATCCTT
GAGTTATATGCTCGAGTATATGAAGAGCTCTTAGCCATTCCTGTTGTGAGAGGAAGGAAGACTGAAAAGG
AGAAGTTTGCAGGAGGAGACTACACAACCACAATAGAAGCCTTCATATCTGCCAGCGGACGCGCCATTCA
GGGAGCAACATCACACCATTTAGGCCAGAATTTCTCCAAAATGTGTGAAATAGTTTTTGAAGATCCCAAG
ACACCAGGAGAAAAGCAGTTTGCATACCAGTGCTCCTGGGGCCTGACAACACGAACCATCGGTGTGATGG
TCATGGTCCATGGGGACAACATGGGCCTAGTGCTTCCACCCCGAGTAGCATCTGTCCAGGTGGTAGTCAT
TCCCTGTGGAATCACAAATGCCCTGTCTGAAGAAGACAGAGAGGCACTGATGGCAAAATGCAACGAGTAC
AGAAGGCGGCTGCTTGGTGCTAACATCCGAGTCCGAGTCGATCTACGAGACAACTACTCCCCAGGCTGGA
AGTTCAACCACTGGGAACTCAAGGGAGTTCCAGTCAGACTTGAGGTGGGGCCACGTGATATGAAGAGCTG
TCAGTTTGTAGCAGTCAGACGAGATACTGGAGAGAAACTGACAATTGCTGAGAAGGAGGCTGAGGCTAAG
CTGGAAAAGGTTTTGGAAGACATCCAGCTGAACCTTTTCACAAGGGCTTCTGAAGACCTTAAAACCCACA
TGGTTGTGTCTAATACACTGGAGGACTTCCAGAAGGTGCTAGATGCTGGAAAGGTTGCACAGATTCCATT
TTGTGGGGAAATTGACTGTGAGGACTGGATCAAAAAGATGACTGCCAGGGATCAAGATGTGGAACCTGGT
GCTCCATCCATGGGAGCCAAAAGCCTTTGCATTCCTTTCAACCCTCTGTGTGAGCTGCAGCCCGGAGCCA
TGTGTGTCTGTGGCAAGAATCCTGCCAAGTTCTACACCTTGTTTGGTCGGAGTTACTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_029735
Insert Size 4539 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_029735.1, NP_084011.1
RefSeq Size 4850 bp
RefSeq ORF 4539 bp
Locus ID 107508
UniProt ID Q8CGC7
Gene Summary Multifunctional protein which is primarily part of the aminoacyl-tRNA synthetase multienzyme complex, also know as multisynthetase complex, that catalyzes the attachment of the cognate amino acid to the corresponding tRNA in a two-step reaction: the amino acid is first activated by ATP to form a covalent intermediate with AMP and is then transferred to the acceptor end of the cognate tRNA (By similarity). The phosphorylation of EPRS, induced by interferon-gamma, dissociates the protein from the aminoacyl-tRNA synthetase multienzyme complex and recruits it to the GAIT complex that binds to stem loop-containing GAIT elements in the 3' UTR of diverse inflammatory mRNAs (such as ceruplasmin), suppressing their translation. Interferon-gamma can therefore redirect, in specific cells, the EPRS function from protein synthesis to translation inhibition (PubMed:23071094). Also functions as an effector of the mTORC1 signaling pathway by promoting, through SLC27A1, the uptake of long-chain fatty acid by adipocytes. Thereby, it also plays a role in fat metabolism and more indirectly influences lifespan (PubMed:28178239).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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