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Top2a (NM_011623) Mouse Untagged Clone
CAT＃: MC224642
Top2a (untagged) - Mouse topoisomerase (DNA) II alpha (Top2a), (10ug)
CNY 19,570.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | Top-2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC224642 representing NM_011623
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTTGTCACCGCTGCAGCCTGTAAATGAAAATATGCTAATGAACAAAAAGAAGAATGAAGATGGCA AGAAAAGATTGTCTATTGAAAGAATCTATCAGAAAAAAACACAACTGGAACATATACTGCTCCGCCCAGA TACCTACATTGGCTCTGTGGAGTTAGTGACCCAGCAAATGTGGGTTTACGATGAAGATGTTGGCATTAAC TACAGGGAAGTCACTTTTGTTCCTGGTTTGTATAAAATCTTTGATGAGATTCTAGTTAATGCTGCTGATA ACAAACAAAGGGACCCAAAAATGTCTTGTATTAGAGTCACAATTGATCCAGAAAATAATGTAATTAGCAT CTGGAATAATGGGAAAGGAATCCCTGTTGTTGAACACAAAGTTGAGAAAATATATGTCCCAGCTCTCATA TTTGGACAACTCCTGACCTCCAGTAACTATGACGACGATGAGAAGAAAGTGACAGGTGGTCGAAATGGCT ATGGAGCTAAACTGTGTAACATATTCAGCACCAAATTTACTGTGGAAACAGCCAGTAGAGAGTACAAGAA AATGTTCAAACAGACGTGGATGGATAACATGGGGAGAGCTGGTGACATGGAACTCAAGCCCTTTAGTGGA GAAGATTATACATGTATCACCTTCCAGCCTGACTTATCTAAGTTTAAAATGCAAAGCCTGGACAAAGATA TTGTTGCACTGATGGTCAGAAGAGCATATGATATCGCTGGCTCCACTAAAGATGTCAAAGTCTTTCTTAA TGGAAATAGTCTGCCAGTGAAAGGATTCCGCAGTTACGTGGATTTGTATTTGAAGGATAAGGTAGATGAA ACTGGGAATTCACTGAAGGTGATACATGAACAGGTCAATCCCCGGTGGGAAGTGTGCTTAACAATGAGCG AGAGAGGCTTCCAGCAGATTAGCTTCGTCAACAGCATTGCGACTTCTAAGGGCGGCAGACATGTCGATTA TGTAGCTGATCAGATTGTGAGCAAACTCGTTGATGTGGTGAAAAAGAAGAACAAGGGCGGGGTTGCAGTG AAGGCTCATCAGGTGAAAAATCACATGTGGATTTTTGTAAATGCCTTAATTGAAAACCCAACCTTTGACT CTCAAACAAAAGAAAACATGACTTTACAAGCCAAGAGCTTTGGATCAACATGTCAATTAAGTGAAAAGTT CATCAAAGCTGCAATTGGTTGTGGTATTGTGGAAAGCATACTAAACTGGGTGAAGTTCAAGGCTCAAATC CAACTGAACAAGAAGTGTTCAGCTGTAAAACATACCAAAATCAAGGGAATCCCCAAACTTGATGATGCCA ACGATGCAGGGAGCCGAAACTCTACTGAATGCACACTTATCCTGACTGAGGGAGACTCAGCAAAAACTCT AGCCGTTTCAGGCCTTGGAGTGGTTGGAAGAGACAAATATGGGGTGTTCCCTCTTAGAGGAAAGATACTC AATGTGCGAGAAGCCTCTCATAAACAGATCATGGAAAATGCTGAAATTAACAATATCATCAAGATTGTGG GTCTCCAGTACAAGAAAAACTATGAAGATGAAGATTCATTGAAAACTCTTCGTTATGGGAAGATCATGAT TATGACAGATCAGGACCAAGATGGTTCCCACATCAAAGGCTTGCTGATCAATTTTATCCATCACAATTGG CCATCTCTTCTGCGACATCGTTTTCTAGAAGAATTTATCACTCCCATTGTAAAGGTGTCCAAAAATAAGC AAGAAATAGCATTCTATAGTCTTCCCGAGTTTGAAGAATGGAAAAGTTCGACTCCGAATCATAAAAAGTG GAAAGTCAAATACTACAAAGGTTTGGGCACCAGCACATCAAAGGAAGCTAAGGAATATTTTGCAGATATG AAACGACATCGTATTCAGTTCAAATACTCTGGCCCTGAAGATGATGCTGCGATCAGCCTGGCCTTTAGTA AAAAACAAGTTGATGATCGAAAGGAATGGTTAACTAATTTTATGGAAGACAGAAGACAGCGGAAGTTACT TGGCCTTCCTGAGGATTATTTGTATGGACAAAGCACTTCGTACCTGACCTATAATGACTTCATAAACAAG GAACTCATCCTCTTCTCCAACTCTGATAATGAAAGGTCTATCCCATCCATGGTAGACGGTTTGAAACCAG GTCAGAGAAAGGTTTTGTTTACTTGTTTCAAACGGAATGACAAGCGAGAAGTGAAGGTTGCCCAGTTAGC TGGGTCAGTGGCAGAAATGTCCTCTTATCACCATGGTGAGATGTCACTGATGATGACCATTATCAATTTG GCGCAGAATTTTGTGGGTAGCAATAATTTGAACCTTTTACAGCCCATTGGTCAGTTTGGAACCAGGCTGC ATGGTGGCAAGGACTCAGCTAGTCCTAGGTACATCTTTACAATGCTCAGCCCTTTGGCTCGGTTGTTGTT TCCTCCAAAAGATGATCACACATTACGGTTTCTATATGATGACAACCAACGTGTTGAGCCTGAATGGTAC ATTCCTATAATACCCATGGTGCTGATAAATGGTGCTGAGGGAATTGGTACTGGGTGGTCCTGCAAAATCC CCAACTTTGATGTGCGTGAAGTTGTGAATAATATCAGACGGCTTCTGGATGGAGAGGAGCCCCTACCCAT GCTCCCAAGTTATAAGAATTTCAAAGGTACTATTGAAGAACTGGCTTCAAATCAGTATGTGATTAATGGA GAAGTAGCTATTCTGGATTCTACAACCATTGAAATCTCAGAGCTTCCCATCCGAACGTGGACTCAGACAT ATAAAGAACAGGTTCTAGAACCCATGTTGAATGGAACTGAGAAGACACCCTCTCTAATAACAGACTATAG GGAATACCATACAGATACCACTGTGAAGTTTGTCATAAAGATGACTGAAGAAAAACTGGCAGAGGCAGAG AGAGTCGGCCTACACAAAGTCTTCAAACTCCAGAGTAGTCTCACTTGCAACTCTATGGTGCTTTTTGACC ATGTAGGTTGCTTAAAGAAATATGACACTGTGTTGGATATTCTAAGAGACTTCTTTGAGCTCAGGCTTAA ATATTATGGATTAAGAAAAGAATGGCTTCTAGGAATGCTTGGTGCAGAATCTTCTAAACTGAATAATCAA GCTCGCTTTATATTAGAGAAAATAGATGGCAAAATAGTCATTGAAAATAAACCTAAAAAAGAATTAATTA AAGTTCTGATTCAGAGAGGCTACGACTCTGACCCTGTGAAAGCCTGGAAAGAAGCTCAGCAGAAGGTTCC AGATGAAGAAGAAAATGAAGAAAGTGACACTGAAACCAGCACCAGTGACTCTGCAGCCGAGGCTGGGCCG ACCTTCAACTACCTTCTCGACATGCCCCTGTGGTATCTGACCAAGGAGAAGAAGGATGAGCTGTGCAAAC AAAGAAACGAGAAGGAACAAGAGCTCAACACATTAAAGCAAAAGAGTCCATCAGATCTGTGGAAGGAAGA TCTGGCTGTTTTTATTGAAGAACTGGAGGTTGTCGAGGCCAAGGAAAAGCAGGATGAACAAGTGGGCCTT CCTGGAAAGGCGGGGAAAGCAAAGGGGAAGAAAGCACAGATGTGTGCTGACGTCTTGCCTTCTCCTCGGG GCAAAAGAGTCATCCCCCAAGTGACTGTGGAGATGAAAGCTGAGGCAGAGAAGAAAATTAGGAAGAAAAT TAAGAGTGAAAACGTTGAGGGTACCCCTGCTGAGGATGGTGCGGAACCAGGAAGCCTCCGGCAACGAATA GAGAAGAAGCAGAAGAAAGAGCCAGGTGCAAAGAAGCAGACTACATTGCCGTTTAAGCCTGTCAAAAAAG GGAGGAAGAAAAACCCCTGGTCTGACTCTGAGTCAGACGTGAGCAGTAATGAAAGTAACGTTGATGTCCC TCCTCGACAAAAAGAGCAAAGAAGTGCTGCAGCAAAAGCCAAATTCACAGTGGATTTAGACTCGGATGAA GATTTCTCAGGTTTGGATGAGAAGGATGAGGATGAAGATTTCCTCCCATTAGATGCTACTCCACCTAAGG CCAAAATCCCCCCAAAAAATACTAAAAAAGCACTGAAGACCCAGGGAAGCTCCATGTCGGTAGTTGATCT TGAAAGTGATGTTAAGGACAGTGTGCCAGCTTCTCCAGGCGTTCCTGCTGCTGACTTCCCAGCGGAAACT GAACAGTCAAAGCCATCCAAAAAGACCGTGGGAGTGAAGAAGACAGCAACCAAAAGCCAGTCTTCAGTCT CCACTGCTGGTACCAAAAAGAGAGCTGCGCCAAAGGGAACCAAATCAGATTCAGCCTTGAGTGCTCGTGT CTCGGAAAAACCTGCTCCTGCCAAAGCCAAGAACAGTCGCAAAAGGAAGCCATCTTCTTCTGATAGCTCT GACTCTGATTTTGAGAGAGCAATTTCTAAAGGTGCCACGAGCAAGAAAGCAAAGGGAGAGGAACAGGACT TCCCTGTGGACTTGGAAGACACGATAGCTCCTCGAGCCAAATCTGACCGGGCGAGGAAGCCAATTAAGTA CCTGGAGGAGTCCGATGACGATGACGACCTCTTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_011623 |
| Insert Size | 4587 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_011623.2, NP_035753.2 |
| RefSeq Size | 5217 bp |
| RefSeq ORF | 4587 bp |
| Locus ID | 21973 |
| UniProt ID | Q01320 |
| Gene Summary | Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes. May play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation (PubMed:24321095).[UniProtKB/Swiss-Prot Function] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG219695 | Top2a (tGFP-tagged) - Mouse topoisomerase (DNA) II alpha (Top2a), (10ug) |
CNY 16,720.00 |
|
| MR219695 | Top2a (Myc-DDK-tagged) - Mouse topoisomerase (DNA) II alpha (Top2a) |
CNY 15,200.00 |
|
| MR219695L3 | Lenti ORF clone of Top2a (Myc-DDK-tagged) - Mouse topoisomerase (DNA) II alpha (Top2a) |
CNY 17,100.00 |
|
| MR219695L4 | Lenti ORF clone of Top2a (mGFP-tagged) - Mouse topoisomerase (DNA) II alpha (Top2a) |
CNY 17,100.00 |
