Gli2 (NM_001081125) Mouse Untagged Clone
CAT#: MC224662
Gli2 (untagged) - Mouse GLI-Kruppel family member GLI2 (Gli2), (10ug)
CNY 19,860.00
Product images
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | AW546128 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC224662 representing NM_001081125
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGACTTCTGCCCCAGCCCCTGCACTGGAGAAGAAAGAAGCCAAGAGTGGTCTCTTGGAGGACAGCA GCTTCCCCGACCCAGGGAAAAAGGCCTGTCCTCTGGCGGTGGCCGCAGCTGTAGCCGCCCACGGAGTTCC TCAGCAGCTCCTGCCGGCTTTCCACGCGCCTTTGCCGATTGACATGAGACACCAGGAGGGAAGGTACCAT TATGACCCTCACTCTGTCCACAGTGTACACGGGCCTCCCACCCTAAGTGGCAGCCCTGTCATCTCAGATA TCTCCTTGATACGACTTTCTCCACACCCTGCTGGCCCTGGAGAGTCACCCTTCAGCGCCCACCACCCCTA CGTGAACCCCCATATGGAGCACTACCTCCGGTCTGTGCACAGCAGCCCCACACTCTCAATGATCTCTGCC GCCAGGGGCCTCAGCCCTGCTGATGTGGCCCACGAACATCTGAAAGAGAGGGGACTCTTTAGCCTCGCAG CCCCAGGCACCAACCCTTCAGACTATTACCACCAGATGACCCTCATGGCAAGCCACCCCACCCCTTATGG GGACCTTCTAATGCAGAGCGGGGGTGCTGCTAGCGCACCCCATCTCCATGACTACCTCAACCCTGTGGAT GCATCACGATTCTCTAGTCCACGTGTGACCCCACGACTGAGCCGCAAGCGGGCTCTGTCCATCTCCCCGC TCTCAGATGCCAGCCTCGACCTACAACGCATGATTCGGACCTCTCCCAACTCGCTGGTAGCTTACATCAA CAACTCCAGGAGCAGCTCAGCAGCCAGTGGCTCTTATGGACATCTGTCTGCTGGTGCCCTCAGCCCAGCC TTCACTTTTCCCCACCCCATCAATCCTGTGGCCTACCAGCAGATCCTGAGCCAGCAGCGGGGCCTGGGCT CAGCCTTTGGACACACACCACCCCTGATCCAGCCTTCACCCACCTTCTTGGCCCAGCAGCCCATGACTCT CACCTCCATCAGCACCATGCCTACCCAACTCAGCAGCAGTAGCAGCAACTGTCTAAATGATGCCAACCAG AACAAGCAGAACAGCGAGTCAGCTGTGAGCAGCACCGTGAACCCCATCACCATTCATAAGCGGAGCAAGG TCAAGACTGAGGCTGAGGGCCTGCGTCCAGCATCCCCGCTTGGACTGACACAGGAGCAGCTGGCCGATCT CAAGGAAGACCTGGACAGGGATGACTGTAAGCAGGAGGCCGAGGTGGTCATCTACGAGACCAACTGCCAC TGGGCAGACTGCACCAAGGAGTATGACACACAGGAGCAGCTGGTGCATCATATCAACAATGAACACATCC ACGGGGAGAAGAAGGAGTTCGTGTGCCGCTGGCAGGCCTGCACGAGAGAGCAGAAGCCCTTCAAGGCCCA GTACATGCTGGTTGTTCACATGCGCAGACACACGGGTGAGAAGCCACACAAGTGCACGTTCGAAGGCTGT TCCAAGGCCTACTCTCGCCTGGAGAACCTGAAGACACACCTGCGTTCACACACAGGAGAGAAGCCATATG TGTGTGAACACGAAGGCTGTAACAAAGCCTTCTCCAATGCCTCAGACCGCGCCAAGCACCAGAACCGCAC TCACTCCAATGAGAAACCCTACATCTGCAAGATCCCAGGCTGCACCAAGAGGTACACAGACCCCAGCTCA CTCCGCAAGCATGTGAAGACTGTCCATGGGCCAGACGCCCATGTCACCAAGAAACAGCGTAATGATGTGC ATGTCCGTGCTCCACTGCTCAAGGAGAATGGGGATAATGAGGCCAGCGCCGAGCCAGGTGGCCGGGGACC TGAGGAGAGTGTGGAGGCCAGTAGCACCAGCCACACTGTGGAGGACTGCCTACATATCAAAGCCATCAAG ACAGAGAGCTCCGGGCTTTGTCAGTCCAGCCCCGGGGCCCAGTCATCCTGCAGCAGCGAGCCCTCTCCCC TGGGCAGTGCCCCCAACAATGACAGTGGCATGGAGATGCCGGGGACAGGGCCTGGGAGTCTGGGAGACCT GACAGCACTGGCTGACACGTGTCCAGGAGCTGACACCTCAGCCCTGGCTGCACCCTCCACTGGTGGCCTG CAGCTGCGCAAACACATGAGCACCGTGCATCGCTTTGAGCAGCTGAAGAGAGAGAAGCTCAAGTCACTGA AGGATTCCTGCTCGTGGGCCGGCCCAGCTCCACACACCCGCAACACCAAGCTGCCTCCCCTTCCAGTCAA TGGTTCTGTCCTGGAAAACTTCAACAATACAGGGGGCGGTGGACCGGCAGGACTGCTGCCCAGCCAGCGG CTACCAGAGCTGACCGAAGTGACGATGCTGAGCCAGCTGCAGGAACGAAGAGACAGCTCCACCAGCACCA TGAGCTCGGCCTACACTGTGAGCCGCCGCTCCTCTGGCATCTCCCCATACTTCTCTAGCCGTCGCTCCAG CGAGGCTTCGCCTCTCGGTGGCCTACGCCCGCACAACGCCAGCTCAGCAGACTCCTATGACCCCATCTCC ACAGATGCCTCTCGGCGCTCCAGTGAAGCCAGCCAGTGCAGTGGCGGTGGCCCAGGGCTGCTCAACCTCA CACCTGCGCAGCAGTACAACCTGCGTGCCAAGTACGCAGCGGCCACAGGTGGACCACCGCCCACGCCACT GCCGGGCCTCGATCGTGTAAGCCTTCGTACCCGCCTGGCCTTGCTGGATGCTCCTGAGCGTGCACTTCCT GGTGCCTGCCCACATCCACTGGGGCCACGGCGTGGCAGCGATGGGCCTACCTATAGCCATGGTCATGGCC ATGGCTACGCAGGTGCGGCTCCAGCATTCCCCCACGAGGGGCCAAACAGCAGCACACGGCGGGCCAGCGA CCCTGTGCGGCGCCCTGACCCCCTTATTCTGCCTCGAGTGCAACGTTTCCACAGTACCCACAACATGAAT CCAGGTTCACTGCCACCCTGCGCTGATCGGCGTGGCCTGCACGTACAGAGCCACCCCAGCGTAGACAGCA ACCTGACCCGCAACGCCTACTCTCCCAGACCCCCTAGCATCAATGAGAACGTGGTGATGGAGGCCGTGGC TGCTGGGGTAGACGGCCCAGGGCTAGAGTGCGACCTGGGGCTGGTGGAGGATGAGCTGGTGCTGCCAGAT GATGTGGTACAGTACATCAAGGCTCACACCGGTGGTACCTTGGATGACGGCATTCGGCAGGGGTATCCCA CAGAAGGTACTGGCTTCCCCGAGAACTCTAAGCTGCCCAGTCCTGGGCTACAAGGCCACCGCAGGCTAGC AGCTGCCGACTCCAACATGGGTCCTTCTGCTCCTGGACTCGGGGGCTGCCAGCTGAGCTACAGCCCCTCC TCCAACCTCAACAAGAGCAACATGCCTGTGCAGTGGAATGAGGTGAGTTCTGGCACCGTGGATGCCCTGC CTACCCAGGTGAAGCCACCTCCTTTCCCTCACAGCAACCTGGCTGTGGTCCAACAGAAGCCAGCCTTTGG CCAGTATCCAGGATATAATCCACAATCCGTGCAGAGCAGCTCCGGAGGTCTAGACAGCACCCAGCCGCAC CTACAGCTTCGAGGAGCCCCCTCTGCATCAAGAGGGAGCTACACGCAACAGCCTCGACAGCCAGCTGCAG GCAGTCAGTGCCTGGGTATGAGTGCGGCCATGAGCCCGCAGGCCAGCTACAGCCAAGCCCACCCCCAGCT GAGCCCAAACATTGTCAGCGGATCTCTGAACCAGTTTTCTCCCTCCTGCAGCAATATGGCAGCCAAGCCC AGCCACCTGGGACTCCCTCAGCAAATGGAAGTTGTCCCCAATGCCACCATCATGAATGGCCATCAACGGG AGCACGGGGTCCCCAATTCATCCCTGGCTGCGGTGTCACAACCTCACCCAGTCCTGAGCTATCCCCAGCA GGACAGCTACCAACAGGGCTCCAACCTTCTGTCATCCCATCAGCCTGGCTTCATGGAGTCCCAGCAGAAC GCGGGCTTTGGTCTCATGCAGCCTCGGCCACCCCTGGAACCCAACACGGCCAGCCGTCACCGTGGAGTAC GTTCTGGGCAACAGCAGTTGTATGCCAGGACCACTGGCCAAGCCATGGTCACATCAGCCAACCAAGAGAC AGCAGAAGCTATGCCCAAGGGACCAGCAGGGACCATGGTATCCCTAGCTCCTCAGCCATCTCAGGACACA GGGCGGGCACAAGATCAGAACACGCTATACTACTATGGCCAGATCCACATGTATGAACAGAATGGAGGCT GCCCAGCCGTGCAGCCCCAGCCGCCACAACCACAAGCTTGCTCAGACAGTATCCAGCCTGAGCCTTTGCC TTCACCGGGAGTCAACCAGGTGTCTAGCACCGTGGACTCCCAGCTCCTGGAGCCCCCCCAGATTGACTTT GATGCCATCATGGATGATGGTGATCACTCGAGTTTGTTTTCTGGTGCACTGAGCCCAACCCTTCTCCACA ATCTCTCCCAGAATTCCTCACGCCTCACCACACCCCGGAATTCCTTGACACTGCCCTCCATCCCTGCGGG CATCAGCAACATGGCCGTGGGCGACATGAGTTCCATGCTCACCAGCCTGGCTGAAGAGAGCAAGTTTTTA AACATGATGACCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_001081125 |
Insert Size | 4635 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001081125.1, NP_001074594.1 |
RefSeq Size | 6708 bp |
RefSeq ORF | 4635 bp |
Locus ID | 14633 |
UniProt ID | Q0VGT2 |
Gene Summary | Functions as transcription regulator in the hedgehog (Hh) pathway (PubMed:9006072). Functions as transcriptional activator (PubMed:10806483). May also function as transcriptional repressor (PubMed:10433919). Requires STK36 for full transcriptional activator activity (PubMed:10806483). Binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the TRE-2S regulatory element (By similarity). Is involved in the smoothened (SHH) signaling pathway (PubMed:10433919). Required for normal skeleton development (PubMed:9006072).[UniProtKB/Swiss-Prot Function] |
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG225917 | Gli2 (tGFP-tagged) - Mouse GLI-Kruppel family member GLI2 (Gli2), (10ug) |
CNY 14,920.00 |
|
MR225917 | Gli2 (Myc-DDK-tagged) - Mouse GLI-Kruppel family member GLI2 (Gli2) |
CNY 10,536.00 |
|
MR225917L3 | Lenti ORF clone of Gli2 (Myc-DDK-tagged) - Mouse GLI-Kruppel family member GLI2 (Gli2) |
CNY 15,490.00 |
|
MR225917L4 | Lenti ORF clone of Gli2 (mGFP-tagged) - Mouse GLI-Kruppel family member GLI2 (Gli2) |
CNY 15,490.00 |