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Depdc5 (NM_001170567) Mouse Untagged Clone
CAT＃: MC224728
Depdc5 (untagged) - Mouse DEP domain containing 5 (Depdc5), transcript variant 3, (10ug)
CNY 20,430.00
货期*
8周
规格
Product images
经常一起买 (3)
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | AV016528 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC224728 representing NM_001170567
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGAACCACAAAGGTCTACAAACTTGTCATCCACAAGAAGGGCTTTGGGGGCAGTGATGATGAGCTAG TCGTGAACCCTAAAGTATTTCCTCACATCAAACTTGGAGACATTGTGGAGATTGCTCACCCCAATGATGA GTACAGTCCTTTGCTTTTGCAAGTCAAGTCGCTTAAGGAAGATTTACAGAAAGAAACTATCAGTGTGGAC CAGACTGTGACTCAAGTATTCCGGCTAAGACCTTATCAAGATGTCTATGTGAATGTTGTAGACCCCAAGG ATGTGACTCTTGACCTGGTGGAATTGACTTTTAAGGATCAGTATATTGGCCGTGGGGATATGTGGCGACT AAAGAAAAGTTTGGTGAGCACCTGTGCCTATATCACTCAAAAAGTGGAATTTGCTGGCATCAGGGCACAG GCTGGTGAGCTGTGGGTCAAGAATGAGAAGGTCATGTGTGGTTACATTAGTGAAGAGACCAGGGTGGTGT TCCGTTCTACGTCGGCTATGGTTTACATATTTATTCAGATGAGCTGTGAAATGTGGGATTTTGATATTTA TGGGGATCTGTACTTTGAGAAAGCTGTGAACGGTTTCCTCGCCGACCTGTTTACTAAATGGAAGGAGAAG AACTGTAGTCATGAAGTGACTGTGGTCCTGTTTTCCAGAACTTTCTATGATGCAAAATCTATTGATGAAT TTCCTGAAATAAACCGAGCTTCAATTCAAGAGGATCACAAAGGGAGATTCTATGAGGACTTTTACAAAGT GGTGGTGCAGAATGAGAGAAGGGAAGAGTGGACTTCACTCCTCGTGACCATTAAAAAACTCTTCATCCAG TATCCAGTGTTGGTGCGACTGGAACAGGCAGGGGGCTTTCCTCAAGGAGACAATTCTACCTCAGCACAAG GAAACTACCTAGAGGCCATCAACTTGTCATTCAATGTGTTTGACAAGCACTACATCAACCGAAACTTTGA CCGAACTGGGCAGATGTCTGTGGTGATCACGCCGGGGGTGGGTGTCTTTGAAGTGGACCGCCTACTCATG ATCTTGACCAAGCAGCGGATGATTGATAATGGAATTGGTGTGGACTTAGTGTGCATGGGAGAGCAGCCAC TACATGCTGTGCCATTATTCAAGCTACATAACCGGAGTGTTCCGAGGGATTCTCGACTGGGTGATGATTA TAATATTCCTCACTGGATAAACCATAGTTTCTACACATCCAAAAGCCAGCTCTTTTGTAACAGTTTCACT CCACGGATAAAACTAGCAGGAAAGAAGTCTGCCTCTGAGAAAACCAAAAATGGTCGTGATACATCTCTCG GGACTCCAAAGGAATCTGAAAACACCCTTCCCATCCAAGTAGATTATGATGCCTACGATGCTCAAGTGTT CAGGCTGCCTGGCCCATCCCGGGCTCAGCGCCTCGCCACCTGCAGGTCTGTGAGAGAACAGGAGAATCAC AGTCGCAAAAGTGCAAGCTCCTGTGATGTCTCATCCAGCCCTTCCCTGCCAAGCCGTGCACTGCCCACTG AGGAAGTGAGGAGCCAGGCTTCTGATGACAGCTCCCTGGGCAAGAGCACCAACATCCTGATGATCCCTAA CCCCCACCTGCACCAGTATGAAGTCAGCAGCTCACTGGGCTACACCAGCACCCGAGATGTCCTGGAGAAC ATGATAGAACCCCCACAGAGGGACTCCAGTGCACCAGGAAGGTTCCATGTGGGTAGTGCAGAGTCCATGC TCCATGTCCGACCTGGAGGATACACGCCTCAGAGAGCGCTGATTAACCCCTTCGCCCCCTCGAGGATGCC CATGAAGCTCACCTCCAACAGAAGGCGCTGGATGCACACTTTTCCTGTAGGCCCATCTGGAGAGGCCATC CAGATCCATCATCAGACCCGGCAAAACATGGCAGAGCTGCAGGGCAGCAGGCAGAGGGACCCTACCCACT CCTCTGCAGAGCTGTTGGAGTTGGCATACCATGAAGCTGCCGGGAGGCACAGCACTTCCCGCCAGCCTGG TGACAGCATGTCCTTGAACTTCAGTGGAACGGAAGAGCTTTCTGTCAGCCTGCTTAGCAACAGCAGTACA GGTGTGAATCCTAGGACCCAGAACAAGGACTCCCTAGAGGACAGTGTTTCTACCTCTCCAGACCCAATGC CAGGCTTCTGTTGCACAGTTGGAGTGGATTGGAAGTCTCTTACTACTCCTGCATGCCTTCCCCTCACCAC TGACTACTTCCCGGACCGTCAGGGCCTGCAGAATGACTACACAGAGGGCTGCTATGATCTCCTCCCAGAA GCAGACATGGACAGGAGGGATGAGGAGGGTGTTCAAATGACAGCCCAGCAAGTGTTTGAAGAGTTCATCT GCCAGCGTCTCATGCAGGGTTACCAAATCATAGTACAGCCAAAGACCCAGAAACCCAACACCACAGTCCC ACCCCCATTGAGCAGTAGCCCACTCTATAGCAGAGGCCTTGTGTCCCGAAATCGCCCTGAGGAGGAAGGC CAGTATTGGTTAAGTATGGGCAGAACTTTCCATAAGGTGACACTCAAGGACAAGATGATCACAGTAACTC GTTACCTTCCCAAGTACCCTTATGAATCTGCCCAGATCCATTACACCTACAGCCTCTGCCCTTCCCACTC TGACTCGGAGTTTGTCTCCTGTTGGGTGGATTTCTGCCACGAACGGCTCGAGGAATACAAGTGGAATTAC TTAGATCAGTATATTTGTTCTGCTGGCTCTGAAGACTTCAGCTTAATCGAGTCTCTGAAGTTCTGGAGAA CCCGATTTTTACTACTGCCAGCCTGTGTTACTGCCACCAAGCGCATCACAGAAGGGGAGGTGCACTGTGA CATCTATGGGGACAAACCCCGTGCAGATGAAGATGAGTGGCAGCTTCTAGATGGCTTTATTCGCTTTGTA GAGGGCTTAAATCGCATCCGCAGGCGCCACCGCTCAGACCGCATGATTCGGAAGGGGACTGCCATGAAAG GCTTGCAGATGACCGGGCCCATCTCTGCACACTCTCTCGAGGCAGCAGGCCCTCCAGTCGGCAAGAAAGG AACCTCAGCTCTGTCTGCCCTGCTGGAGATGGAAGCTAGTCAGAAGAGCCTAGGAGAACAGCAGACGACT GTGCACGGAAAAAGCTCCACTCAGCCAGCTGAGAACAGCAGTGTTGCCATGACTCCCACCTATGTGGACA GCCCACGCAAGGACGGGGCCTTCTTTATGGAGTTTGTCCGCAGCCCACGCACAGCATCATCTGCCTTCTA CCCTCAGGCATCTGTGGACCAAACAGCTCCTCTGGTGTTAGACAGCACCAGTTTAGGGGTCAGCACAGGC CAGCCCATGGATAGAGGCAACAACCAGACCTTTGGGAACTCCCAGAACATAGAACAGGCCTTTCCCTCTG CAAACTCTGGTGACTACAGCTCTCAGCAGCATGTAGCAAGCTCTCTAACATCGTCTTCTACCCTGGTGGA GATTCTGGAAGCCATGAAGCATCCCTCGACAGGAGTCCAGCTGCTGTCTGAACAGAAGGGCCTTTCACCG TGCTGCTTCATCAGTGCTGAGGTCGTACACTGGTTGATGAACAACGTGGAGGGGGTCCAGACGCAGGCGA TGGGCATAGACATCATGCAGAAAATGCTGGAAGAGCAGCTCATAACGCATGCATCTGGTGAAGCATGGCG GACCTTCATCTATGGCTTCTATTTCTACAAGATAGTAATGGACAAAGAACCGGAAAGAGTGGCTATGCAG CAGCCCTCTGCCCCCTGGTACACAGCAGGAGCGGATGACTTTGCCAGCTTCCAACGCAAGTGGTTTGAGG TGGCCTTTGTGGCAGAAGAGCTTGTGCACTCTGAGATTCCTGCCTTCCTCCTGCCCTGGCTACCTAGCCG GCCAGCCTCTTATGCAAGCAGACACAGCTCCTTTAGCCGAAGTTTTGGAGGACGGAGCCAGGCAGCTGCG CTGTTAGCTGCTACTGTCCCAGAGCAGAGGACTGTGACCCTGGATGTTGATGTGAACAACCGAACGGACC GGCTGGAATGGTGCAGCTGTTACTACCATGGAAACTTCTCTCTCAATGCCGCCTTTGAGATCAAGCTACA CTGGATGGCAGTGACTGCCACAGTGCTGTTTGAGATGGTCCAAGGTTGGCATCGAAAAGCTACCTCCTGT GGCTTCTTGCTGGTCCCAGTTTTGGAGGGTCCTTTTGCTCTGCCCAGTTACCTGTATGGTGATCCCCTGA GGGCCCAGCTCTTCATCCCGCTCAACCTCAGCTGCTTGCTCAAGGAGGGCAGCGAGCACCTGTTCGATAG CTTTGAACCAGAGACATATTGGGATCGAATGCATCTTTTCCAGGAAGCCATTGCACACAGGTTTGGATTT GTGCAAGATAAATATTCTGTCTCCGCTTTTAACTTCCCTGCTGAGAATAAGCCACAGTATATCCATGTCA CAGGAACGGTGTTCCTTCAGCTGCCGTACTCCAAACGCAAGTTTTCAGGGCAGCAGCGGCGGCGACGGAA CTCTACCAGCTCCACCAACCAAAACATGTTCTGTGAGGAGCGGGTTGGCTACAACTGGGCCTACAACACC ATGCTGACCAAGACATGGCGCTCTAGTGCCACAGGGGACGAGAAGTTTGCTGATCGGCTGCTGAAGGACT TCACGGATTTCTGCATCAACCGTGACAACCGACTGGTCACGTTCTGGACAAACTGCCTGGAGAAGATGCA TGCCAGTGCTCCATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001170567 |
| Insert Size | 4776 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001170567.1, NP_001164038.1 |
| RefSeq Size | 7944 bp |
| RefSeq ORF | 4776 bp |
| Locus ID | 277854 |
| UniProt ID | P61460 |
| Gene Summary | As a component of the GATOR1 complex functions as an inhibitor of the amino acid-sensing branch of the TORC1 pathway. The GATOR1 complex strongly increases GTP hydrolysis by RRAGA and RRAGB within RRAGC-containing heterodimers, thereby deactivating RRAGs, releasing mTORC1 from lysosomal surface and inhibiting mTORC1 signaling. The GATOR1 complex is negatively regulated by GATOR2 the other GATOR subcomplex in this amino acid-sensing branch of the TORC1 pathway.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 5' UTR, uses an alternate in-frame splice site and includes an alternate exon in the 3' coding region, which results in a frameshift, compared to variant 1. The encoded isoform (3) has a distinct C-terminus and is longer than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG217427 | Depdc5 (tGFP-tagged) - Mouse DEP domain containing 5 (Depdc5) transcript variant 3, (10ug) |
CNY 17,480.00 |
|
| MR217427 | Depdc5 (Myc-DDK-tagged) - Mouse DEP domain containing 5 (Depdc5), transcript variant 3 |
CNY 15,870.00 |
|
| MR217427L3 | Lenti ORF clone of Depdc5 (Myc-DDK-tagged) - Mouse DEP domain containing 5 (Depdc5), transcript variant 3 |
CNY 17,770.00 |
|
| MR217427L4 | Lenti ORF clone of Depdc5 (mGFP-tagged) - Mouse DEP domain containing 5 (Depdc5), transcript variant 3 |
CNY 17,770.00 |
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