Shprh (NM_172937) Mouse Untagged Clone
CAT#: MC224760
Shprh (untagged) - Mouse SNF2 histone linker PHD RING helicase (Shprh), transcript variant 2, (10ug)
CNY 20,710.00
Product images
![](https://cdn.origene.com/img/defaults-img.jpg)
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | 2610103K11Rik; AA450458; AU024614; BC006883; D230017O13Rik; E130018M05 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC224760 representing NM_172937
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCAGCCGAAGGAAACGTGCTCCCCCGATGAAGGTCGATGAAGAAAGGCAACAGCAGCTTCACTGGA ACATGCATGAGGACCTGAGGAGTGAGCCGCTCACCATGACGGTCGGGGAACAGGCCTGTTCAGATGCAGA CTCTTCTTCTGATTGCATCATCATCGATGAAGGCCCTCCTGAGAGTGCACTTCACAGAGATAAGAAGAGG CGTTCAGAGACTGTGAGCGTCTTAGAGGCCACCGAGGAAGAGACCCGTTTGTCTGTGACTTTGAATGTTA CAGTTTCTCCCTATCGTGTGGATAATTCTTGGAAAGCATTTCTGGGAGATTTTGCTCTTCAGCTTCTCCC CAAAGAGAGTTTAGTTGAACATTTTTCTGAAAGGACTTTTACATTGAGTCCTTCAGAGTCAAGCAGTCAG TTCCTGATCTATGTTCATTCAGAATGTAAAAATGTAGAGAAACAAGAAAATGTTCTTGAAGGATCAGCCG GTGTTTGTAGCAAGGGAATCCGAGTGGAATCCTCCTTCAGTAGTGACATGTTACAGGATTTGGCCTGGCT ACAGAAGAGAAGAGGAATAAAACTCTACCAGAGACCAGACGGCACTCACACAATCAAGGTTGGAATTTAC ATTTTGGAAGCTGGCCTAACAAGACTAGACTTCATGAGTGATGCAGGTTCCAGAATGAAAAAGTTCAATC AGCTCATGAAGAGAGTGATGGAAAAGTTACATAATTTCATTATTCCAGATGTGCTGGAAGAAGAGGAGGA GGGTTCAGAGAGCGAGCCCGAGGGTCAGGATATTGATGAGCTCTATCACTTTGTGAAGCAAACACACCAG CAAGAAACACGGTCAGTCCAAGTGGACGTCCAGCACCCTGCGTTGATTCCGGTCCTGAGACCTTACCAGA GAGAGGCAGTCAACTGGATGCTGCAGCAAGAGCAGTTCAGAAGCGCTCCTCCTGCTGATAACTCCCTGCA CTTCTTATGGCGAGAAATTGTTACACCTGATGGCTTGAAACTCTACTATAATCCATATACAGGCTGCATC ATTCGAGATTTCCCACATGCGGGTCCACAGTTGCTTGGTGGAATCTTAGCAGATGAGATGGGTCTTGGAA AGACAGTGGAGGTTCTGGCTCTGATTCTGACACATACCCGACAAGACGTCAAGCAGGACGCTCTCACCCT TCCTGAGGGAAAAGTGGTAAATTATTTCATCCCAACTCACTGTCCTAGAGAAAAAGTGAAAAATAGAGAA ATCCAGGATACAGAATATGAACCAAAAGAGAAAGTTCACTGCCCTCCTACACGTGTGATGATCCTGACAG CTGTGAAAGAAATGAATGGGAAGAAAGGAGTTTCTATTCTTTCTATCTATAAGTATGTCAGCTCCATATT CAGATATGATGTTCAGCGGAACCGGGGTCTTCTGAAGCGGATGTTGAAATGTTTAATCTTTGAAGGTCTT GTGAAACAGATCAAAGGCCATGGTTTCTCTGGGACTTTTACACTGGGGAAGAATTACAAAGAAGATGTAT TTGATAAAACAAAAAAGCAGGCAGTAGGGAGTCCAAGGAAAATTGAGAAGGAATTGAGGAAATCAGTGAA CAAGGATGCAGATTCTGAGTACCTGCCATCAAACACTTCTGATGACGATGAGCCTTACTATTATTACTGT AAGGCGGGAAAAAGCCGGAGTAAGTTGAAGAAGCCTGCTTTACTAACTAAAAAGGGAAAGGGTCAGTCTG TTCATCTGGATTCCCAAGGTGATGCTCCAGCTGCTGGAGTCTGTGCAAGTACTGATGTTCATGTGTCAGA AAATACATGTGTCTCTGAAGACAAGCAAACCCAAGAAGCAAAAGACTGTGCTGAGTCGCCAAACCCTGCT GCCGAGGAGTTGGCACAGTCTAACACTTCAAGTCCTTGTGAAACCTCTGATTACCGCTTTGAGTGTATAT GTGGTGAATTTGATCAAATTGGCCACAAGCCAAGAGTTCAATGTCTGAAGTGTCACCTGTGGCAGCATGC AAAGTGTGTGAATTATGAGGAGAAAAACCTGAAGGTTAAGCCTTTTTACTGCCCCCACTGTCTTGTTGCG ATGGAGCCAGTATCAACAAGAGCAACTCTGATCATCTCTCCGAGCTCCATTTGCCACCAGTGGGTGGATG AAATCAATCGGCACGTGAGGTCATCATCTCTCCGAGTTCTGGTATATCAAGGAGTGAAGAAACACGGCTT CTTACAGCCTCATTTTTTGGCAGAACAAGATATAGTTATCATTACCTATGATGTTCTTCGTTCAGAACTA AACTATGTCAATATCCCACATAGCAATAGTGAAGATGGGCGTCGCCTGAGGAACCAGAAGCGCTATATGG CCATTCCCAGCCCCTTGGTAGCGGTGGAATGGTGGAGGATCTGCCTTGATGAAGCTCAGATGGTCGAGTG TCCTACAGTAAAAGCCGCGGAAATGGCCCAGCGTTTGAGCGGGATTAATCGGTGGTGCATCAGTGGCACT CCAGTTCAGAGAGGATTGGAAGATCTTTTTGGGCTGGTGGTCTTTCTTGGAATTGAACCTTATTGTGTCA AACACTGGTGGATTCGGCTTCTCTATCATCCATACTGCAAGAAAAATCCTCAGCACCTGTACAGTTTTAT TGCCAAGATAATGTGGAGGTCTGCAAAGAAAGATGTCATTGACCAAATCCAGATTCCACCTCAGACTGAA GAAATGCACTGGCTCCATTTTTCCCCGGTGGAGAGGCATTTCTATCACCGCCAGCACGAGGTGTGCTGCC AGGATGCCATAGTGAAGCTCAGGAAGATCTCTGACTGGGCCCTGAAGCTCAGCAGCTTGGACAGGAGAAC TGTCTCCTCCATCCTCTACCCACTGCTGAGGCTCCGGCAGGCCTGCTGCCATCCACAGGCTGTTCGTGGG GAATTCTTGCCACTCCAGAAGAGCACCATGACAATGGAAGAGTTGCTGACATCTCTGCAGAAGAAATGTG GAACGGAATGTGAAGAGGCCCACCGACAACTAGTTTGTGCCCTTAACGGCTTAGCAGGCATTCACATCAT TAAAGGTGAATATGCCTTGGCAGCGGAGCTCTACAGAGAAGTATTACGTTCATCTGAGGAGCACAAAGGA AAACTCAAAACTGATTCCCTTCAAAGACTGCATGCCACACATAACTTAATGGAGCTGTTGGGAGCCAAAC ACCCAGGAATACCTCCCACCTTGAGAGATGGCAGACTTGAAGAAGAGGCCAAGCAGCTGCGAGAGCACTA TATGAGTAAGTGTAACACAGAAGTTGCCGAAGCCCAGCAAGCTTTGCAGCCTGTGCAGCAGTCCATTAGG GAGCTCCAAAGGAAGATTCATTCTAATTCTCCTTGGTGGCTGAATGTAATCCATAGAGCAATGGAATTTT CTGTTGATGAGGAACTTGTTCAGCGGGTGCGAAATGAAATAAGCAGTAACTACAAGCAACAAACTGACAA GCTGTCTATGTCAGAGAAGTTCCGTGACTGCAGAGGTCTTCAGTTCTTACTTACAACACAAATGGAAGAG CTCCATAAGTTCCAGAAGCTGGTGAGGGAGGCTGTGAAGAAGCTGGAGAAGCCTCCATCCCGGGAGGTGA TTGAGTCTGCAACAGTTTGCCACCTGCGGCCAGCTAGACTCCCTCTCAACTGCTGCGTCTTTTGTAAAGC TGATGAATTGTTCACAGAGTATGAATCAAAGCTATTTTTTAACACAGTCAAAGGCCAGACTGCAATCTTT GAAGAAATGATAGAAGATGAAGAAGGACTTGTAGATGATAGAGTACCTACCACTACCAGAGGTCTGTGGG CCGTAAGTGAGACGGAGCGATCTATGAAAGCAATATTATCATTTGCAAGATCACATAGGTTTGATGTTGA ATATGTAGATGAAGGAAGTGTTTCAATGGATCTCTTTGAGGCATGGAAGAAGGAATATAAGTTACTTCAT GAATACTGGATGACTCTGAGAAATCGTGTATCTGCTGTTGATGAACTTGCAATGGCTACAGAGCGACTAA GAGTGCGCCATCCCAAAGAGCCAAAGCCAAACCCACCCGTCCATCACATCATTGAACCACACGAGGTTGA ACAGAATCGTATAAAACTGGTGAATGATAAAGCTGTTGCTACATCACAGCTTCAGAAGAAACTTGGACAG CTTCTTTACCTAACGAATTTGGAGAAGTCTCAAGATAAAACATCAGGAGGTATTAATCCAGAGCCTTGTC CAATCTGTGCTCGACAGCTGGGAAAACAGTGGGCAGTGCTGACCTGCGGACACTGCTTCTGTAATGAATG CACTTCGATTATAATTGAACAGTACAGTGTGGGGTCTCATCGAAGTTCCATCAAGTGTGCTATCTGCCGC CAGACCACATCACACAAAGAAGTCTCCTATGTCTTCACCTCAGAGAAGGCAAACCAAGAGGATGATATCC CTGTGAAGGGTAGCCATTCTACAAAAGTGGAAGCTGTGGTCAGAACTCTGATGAAAATACAACTTAGAGA TCCAGGGGCCAAAGCGCTCGTTTTCTCAACGTGGCAAGATGTATTAGATATTATTTCAAAAGCTCTCACG GACAACAACATGGAATTTACACAAATCAGTCGCATTAAGACATTCCAGGAGAACCTCTCAGCGTTTAAAT ACGATCCCCACATCAATATTCTGCTGCTGCCCCTGCACACAGGCTCTAATGGATTAACTATCATTGAAGC CACCCATGTCCTCCTGGTGGAGCCCATACTGAACCCTGCCCATGAGCTGCAGGCCATTGGGAGAGTGCAC CGAATTGGACAGACCAAGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_172937 |
Insert Size | 4851 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_172937.3, NP_766525.3 |
RefSeq Size | 7385 bp |
RefSeq ORF | 4851 bp |
Locus ID | 268281 |
UniProt ID | Q7TPQ3 |
Gene Summary | E3 ubiquitin-protein ligase involved in DNA repair. Upon genotoxic stress, accepts ubiquitin from the UBE2N-UBE2V2 E2 complex and transfers it to 'Lys-164' of PCNA which had been monoubiquitinated by UBE2A/B-RAD18, promoting the formation of non-canonical poly-ubiquitin chains linked through 'Lys-63'.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks two exons and its transcription extends past a splice site that is used in variant 1, resulting in an immediate translation termination and a distinct 3' UTR compared to variant 1. It encodes isoform b which is truncated at the C-terminus, compared to isoform a. |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG217972 | Shprh (tGFP-tagged) - Mouse SNF2 histone linker PHD RING helicase (Shprh) transcript variant 2, (10ug) |
CNY 17,770.00 |
|
MR217972 | Shprh (Myc-DDK-tagged) - Mouse SNF2 histone linker PHD RING helicase (Shprh), transcript variant 2 |
CNY 16,150.00 |
|
MR217972L3 | Lenti ORF clone of Shprh (Myc-DDK-tagged) - Mouse SNF2 histone linker PHD RING helicase (Shprh), transcript variant 2 |
CNY 18,050.00 |
|
MR217972L4 | Lenti ORF clone of Shprh (mGFP-tagged) - Mouse SNF2 histone linker PHD RING helicase (Shprh), transcript variant 2 |
CNY 18,050.00 |