Pappa (NM_021362) Mouse Untagged Clone

CAT#: MC224767

Pappa (untagged) - Mouse pregnancy-associated plasma protein A (Pappa), (10ug)



  "NM_021362" in other vectors (4)

CNY 20,810.00


货期*
9周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 8430414N03Rik; IGFBP-4ase; PAG1; PAPP-A
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC224767 representing NM_021362
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGGCTCTGGAGTTGGGTGCTGCGCCTGGGGCTGCTGAGCGCCGCGCTGGGCTGCGGGCTGGCCGAGC
GCCCCCGCCGGGTCCGAAGAGACCCTCGGGCCGTGCGCCCCCCGCGCCCCGCCGCTGGACCGGCCACCTG
CGCCACCCGGGCGGCCCGCGGTCGCCGCGCCTCGCCGCCGCCGCCTCCGGGCGGTGCCTGGGAAGCCGTG
CGCGTCCCCCGGCGGCGGCAGCAGCGGGCGGCGAGGGGCGCCGAGGAGCCGAGCCCGCCTAGCCGGGCGC
TCTATTTCAGCGGGCGAGGGGAGCAGCTGCGCCTCCGGGCCGACCTGGAGCTACCCCGCGACGCCTTTAC
ACTGCAAGTGTGGCTGCGAGCCGAGGGTGGCCAGAAGTCTCCAGCAGTGATCACAGGGCTGTATGACAAA
TGTTCTTATACCTCACGTGATCGAGGATGGGTCATGGGCATTCACACCACCAGTGATCAAGGCAACAGAG
ATCCACGCTACTTTTTCTCCTTGAAGACAGACCGGGCCAGGAAAGTGACCACCATTGATGCCCATCGCAG
CTACCTCCCAGGTCAGTGGGTACATCTAGCTGCTACCTATGATGGGCGGCTGATGAAGCTCTATATGAAT
GGTGCCCAGGTGGCAACTTCGGCTGAGCAAGTAGGTGGCATATTCAGCCCACTGACCCAGAAGTGTAAAG
TGCTCATGTTGGGGGGCAGTGCTCTGAATCACAACTTCCGGGGCCACATTGAACACTTCAGTCTATGGAA
AGTAGCAAGAACCCAGCGAGAGATTGTATCCGACATGGAAACCCGTGGCCTCCACACCCCTCTACCTCAG
CTCCTCCTCCAGGAGAACTGGGACAACGTGAAGCGCACTTGGTCCCCCATGAAGGATGGCAACAGCCCCC
AGGTGGAATTCAGCAATGCCCACGGCTTCCTGTTGGACACTAATTTGGAGCCCCCTCTTTGTGGGCAGAC
ACTGTGTGACAACACAGAAGTCATCTCCAGTTACAATCAGCTCCCAAGTTTTCGGCAGCCCAAGGTGGTC
CGCTATCGTGTGGTCAACATCTATGATGATCACCATGAGAATCCAACGGTGAGCTGGCAACAGATTGACT
TTCAGCACCAACAGCTGGCTGAGGCCTTTCAACACTACAACATCTCCTGGGAGCTGGAGGTACTGAATAT
AAACAGTTCCTCTCTGCGTCACCGCCTCATCCTAGCCAACTGTGACATCAGCAAGATTGGGGATGAAAAA
TGTGATCCAGAATGTAACCATACACTGACTGGTCATGATGGTGGAGATTGCCGCCAGCTGCGCTACCCTG
CGTTCATGAAGAAGCAGCAGAATGGTGTGTGTGACATGGACTGTAACTACGAAAGGTTTAATTTTGATGG
TGGAGAGTGCTGTGACCCAGACATCACTGATGTCACTAAGACATGCTTTGATCCTGACTCTCCACACAGA
GCCTACTTGGATGTTAATGAGCTAAAGAACATTCTTAGACTGGACGGATCAACACATCTCAATATTTTCT
TTGCAAACTCTTCAGAGGAGGAGTTGGCAGGAGTGGCAACTTGGCCATGGGACAAGGAAGCCCTAATGCA
CTTGGGCGGTATTGTCTTGAACCCATCTTTCTATGGCATTCCCGGACACACCCACACCATGATTCATGAG
ATTGGGCATAGCCTGGGCCTCTATCACATCTTCCGTGGCATCTCAGAAATCCAGTCCTGCAGTGATCCCT
GCATGGAGACAGAGCCTTCATTTGAAACTGGAGACCTCTGCAATGATACCAACCCAGCCCCCAAACACAA
GTTTTGTGGAGACCCTGGACCAGGGAATGACACTTGTGGCTTTCATGGCTTCTTCAACACTCCTTACAAC
AACTTCATGAGCTACGCAGATGACGACTGTACAGACTCTTTCACGCCCAATCAAGTCTCCAGAATGCACT
GTTACCTGGACCTCGTATACCAGAGCTGGCAGCCCTCCAGAAAGCCAGCACCTGTAGCTCTTGCGCCCCA
GGTTGTGGGGCACACAATGGACTCTGTGATGCTAGAGTGGTTCCCACCCATCGATGGCCACTTCTTTGAA
AGAGAATTGGGATCAGCATGTGACCTTTGCCTAGAAGGGAGAATCCTGGTGCAATATGCTTTCAATGCCT
CCTCCCCCATGCCCTGTGGACCGTCAGGACACTGGAGTCCTCGGGAAGCAGAAGGTCACCCAGATGTTGA
ACAGCCCTGTAAATCCAGTGTCCGAACCTGGAGTCCAAATTCAGCTGTCAACCCACACACAGTTCCTCCA
GCCTGCCCTGAGCCACAAGGCTGCTACCTCGAGCTGGAATTTCGCTACCCTTTGGTCCCTGAGTCTCTGA
CCATCTGGGTAACCTTTGTCTCCAGTGATTGGGACTCTAGTGGAGCTGTCAATGACATCAAACTCTTGAC
TATCAGTGGAAAGAATATCTCTTTGGGTCCTCAGAATGTTTTCTGTGATATCCCACTTACCATCAGACTC
CGGGATGTGGGTGAGGAGGTATATGGCATCCAAATCTATACTCTTGATGAGCACCTGGAGATTGATGCAG
CAATGCTGACCTCCACTGTAGACAGTCCACTCTGCCTACAGTGTAAACCTCTGCAGTATAAAGTGCTTCG
AGACCCACCTCTGCTAGAAGATGTAGCCTCATTACTCCACCTCAACAGAAGATTCATGGACACGGATCTG
AAACTTGGCAGTGTGTACCAGTACCGGATTATCACCATTTCAGGAAATGAAGAGAGCGAGCCATCACCTG
CTGCCATATACACCCACGGAAGTGGGTACTGTGGTGATGGCGTTATCCAAAAAGACCAAGGAGAAGAATG
TGACGACATGAATAAGGTCAATGGGGATGGCTGCTCCCTTTTCTGCAAGCAAGAAGTTTCCTTCAACTGC
ATTGATGAACCCAGCCGGTGCTATTTCCATGATGGGGATGGGATGTGTGAAGAGTTTGAACAAAAAACTA
GCATTAAAGACTGTGGTGTCTACACGCCCCAGGGTTTCCTGGATCAGTGGGCATCCAATGCTTCAGTATC
TCATCAAGACCAGCAGTGCCCAGGTTGGGTTGTCATTGGGCAGCCAGCGGCATCTCAGGTGTGTCGAACC
AAGGTGATAGATCTCAGTGAAGGCATTTCCCAGCATGCTTGGTATCCTTGCACCATTACTTACCCATACT
ACCATCTGCCTCAGACCACATTCTGGCTCCAGACATATTTCTCTCAGCCAATGGTTGCTGCAGCTGTAAT
TATTCACCTGGTGACTGATGGGACATACTATGGGGACCAAAAGCAAGAGACCATCAGTGTGCAGTTGCTT
GATACCAAAGATCAAAGCCATGATCTAGGCCTCCATGTCTTGAGCTGCAGAAACAATCCCCTGATTATCC
CTGTGGTCCATGACCTCAGCCAGCCCTTCTACCACAGCCAGGCGGTACATGTGAGCTTCAGTTCGCCCCT
GGTCGCCATCTCGGGGGTGGCCCTCCGCTCTTTCGACAACTTTGACCCCGTCACCCTGAGCAGCTGCCAG
AGAGGAGAGACCTACAGCCCTGCTGAGCAGAGCTGTGTGCATTTTGCCTGTCAAGCTGCCGACTGCCCAG
AACTGGCCGTGGGGAATGCTTCTCTCAACTGTTCCAGCAACCACCACTACCATGGTGCCCAGTGCACTGT
GAGCTGCCAGACAGGTTATGTGCTGCAGATACAGCGGGACGATGAGCTAATCAAGAGCCAGGTAGGGCCA
AGCATCACAGTGACATGTACCGAGGGCAAATGGAACAAGCAGGTGGCATGTGAGCCGGTGGACTGTGGTA
TCCCAGATCACCATCACGTCTATGCTGCCTCCTTCTCCTGTCCAGAGGGTACCACCTTTGGTAGAAGATG
TTCTTTTCAGTGTCGCCACCCTGCCCAGCTGAAAGGCAACAACAGCTTTCTGACCTGTATGGAAGATGGA
CTGTGGTCCTTCCCAGAGGCCTTGTGTGAGCTCATGTGCCTCGCCCCACCCCCAGTTCCCAATGCGGACC
TACAGACAGCCCGGTGTCGAGAGAACAAGCACAAGGTGGGCTCCTTCTGCAAGTACAAGTGTAAACCTGG
ATACCACGTGCCTGGCTCATCTCGGAAGTCCAAGAAACGGGCTTTCAAGACTCAATGTACTCAAGATGGC
AGCTGGCAAGAGGGAACTTGTGTGCCGGTGACTTGTGACCCACCTCCACCCAAATTCCATGGGCTCTATC
AATGCACTAATGGCTTCCAGTTCAATAGTGAGTGCAGGATCAAGTGTGAAGACAGTGATGCCTCCCAGGG
CCGTGGGAGCAATATCATTCACTGCCGGAAAGATGGCACTTGGAGTGGTTCCTTCCACGTCTGCCGAGAG
ATGCAAGGCCAGTGCTCAGCCCCAAACCAACTCAACAGTAACCTCAAATTGCAGTGTCCTGATGGCTATG
CAATAGGGTCAGAGTGTGCCATCTCGTGCCTGGACCATAACAGCGAGTCCATCATCCTTCCCGTTAACTT
GACAGTGCGTGACATACCCCATTGGATGAACCCCACACGAGTACAGAGGATTGTCTGCACTGCTGGTCTC
CAGTGGTATCCCCACCCTGCTCTGATCCACTGTGTCAAAGGCTGTGAGCCATTCATGGGAGACAACTACT
GTGATGCCATCAACAATCGAGCCTTCTGCAACTATGATGGTGGGGACTGCTGCACCTCCACAGTAAAGAC
CAAAAAGGTCACTCCCTTTCCTATGTCCTGTGACCTACAAAATGACTGCGCCTGTCGAGACCCTGAGGCC
CAAGAACACAACCGGAAAGATCTTCGGGGATATAGCCATGGCTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_021362
Insert Size 4875 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_021362.1, NP_067337.1
RefSeq Size 11027 bp
RefSeq ORF 4875 bp
Locus ID 18491
UniProt ID Q8R4K8
Gene Summary Metalloproteinase which specifically cleaves IGFBP-4 and IGFBP-5, resulting in release of bound IGF. Cleavage of IGFBP-4 is dramatically enhanced by the presence of IGF, whereas cleavage of IGFBP-5 is slightly inhibited by the presence of IGF. Isoform 2 cleaves IGFBP-4 very slowly compared to PAPP-A, but its ability to cleave IGFBP-5 is unaffected.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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