Akap12 (NM_031185) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI317366; Srcs5; SSeCKS; Tsga12
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224827 representing NM_031185 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTGCAGGCAGTTCCACCGAGCAGCGGAGCCCCGAGCAGCCGGCGGAGAGCGACACGCCGAGCGAGC TGGAGCTCAGTGGCCATGGGCCCGCAGCGGAAGCGTCGGGAGCAGCTGGAGATCCCGCTGACGCGGACCC CGCCACCAAGCTCCCACAGAAGAATGGTCAGCTGTCTGCCGTCAATGGTGTAGCTGAACAAGAAGATGTC CACGTCCAAGAGGAAAGCCAGGATGGGCAAGAGGAAGAAGTCACTGTTGAAGATGTTGGACAGAGAGAGT CAGAAGATGTGAAAGAAAAAGACCGAGCTAAAGAAATGGCAGCCAGTTCCACAGTTGTTGAAGATATCAC AAAGGACGAGCAGGAGGAAACACCGGAAATAATCGAACAGATCCCTGCTTCAGAGAGCAATGTGGAAGAA ATGGCGCAGGCTGCTGAGTCCCAAGCTAATGACGTCGGCTTCAAGAAGGTATTTAAATTTGTTGGTTTTA AATTCACGGTGAAGAAGGATAAAAACGAAAAGTCAGATACCGTCCAGCTACTCACTGTCAAGAAGGATGA AGGCGAAGGGGCAGAAGCCTCCGTCGGAGCAGGAGACCACCAAGAGCCCGGAGTGGAGACCGTCGGCGAA TCAGCATCCAAAGAAAGTGAGCTGAAGCAATCCACAGAGAAGCAAGAAGGCACCCTGAAGCAAGCACAGA GCAGCACAGAAATTCCCCTTCAAGCCGAATCTGGTCAAGGGACCGAGGAAGAAGCAGCCAAAGATGGAGA AGAAAACCGAGAGAAAGAACCTACCAAGCCCCTAGAATCTCCGACCAGCCCTGTCAGCAATGAGACAACA TCTTCCTTCAAGAAATTCTTCACTCACGGCTGGGCCGGCTGGCGCAAGAAGACCAGCTTCAAGAAACCAA AGGAAGATGATCTGGAAACTTCCGAGAAGAGAAAGGAGCAAGAGGCTGAAAAAGTAGACGAGGAAGAAGG GGAAAAGACAGAGCCAGCCCCAGCCGAGGAGCAGGAGCCTGCAGAAGGCACAGACCAGGCCAGGTTGTCA GCCGACTATGAGAAGGTGGAGTTGCCTTTGGAAGACCAGGTCGGTGACCTGGAGGCATTGTCGGAGAAGT GTGCTCCTTTGGCAACGGAAGTGTTTGATGAGAAGACGGAAGCCCACCAAGAAGTTGTTGCAGAGGTCCA CGTGAGCACCGTGGAGAAGATGACGAAAGGGCAAGGAGGAGCAGAGGTGGAAGGGGATGTGGTGGTGGAA GGATCGGGAGAATCCTTGCCCCCTGAGAAACTGGCTGAGACCCAGGAGGTCCCCCAGGAAGCTGAGCCTG TGGAGGAGCTGATGAAGACCAAAGAAGTATGCGTCTCTGGGGGTGACCATACTCAGCTGACAGATCTAAG TCCTGAAGAGAAGATGCTACCCAAACACCCCGAAGGCATTGTCAGTGAGGTGGAGATGCTGTCCTCTCAG GAGAGAATCAAGGTACAGGGAAGTCCCCTGAAGAAGCTCTTCAGCAGTTCGGGCTTAAAGAAGCTCTCCG GGAAGAAGCAGAAGGGGAAGAGAGGAGGAGGCGGGGGAGATGAAGAGCCAGGAGAATACCAACACATTCA AACCGAGTCCCCAGAGAGTGCTGACGAGCAGAAGGGAGAGAGCTCTGCCTCTTCCCCTGAAGAGCCCGAG GAGATCGCGTGTCTGGAGAAGGGGCCATCGGAAGCACCCCAGGAAGCGGAAGCTGAGGAAGGAGCGACTT CCGACGGAGAGAAGAAAAGGGAAGGGATCACCCCCTGGGCATCCTTCAAAAAGATGGTGACACCCAAGAA ACGGGTCCGAAGACCTTCTGAGAGCGACAAGGAAGAAGAGCTGGATAAGGTCAAGAGTGCCACCTTGTCC TCCACGGAGAGCACGGCGTCTGGAATGCAGGATGAGGTCAGAGCGGTTGGCGAGGAGCAAAGGTCAGAGG AGCCAAAGCGCAGGGTGGATACTTCAGTGTCTTGGGAGGCGTTGATTTGTGTCGGATCGTCCAAGAAGAG AGCGAGGAAGGCATCCTCTTCAGATGATGAAGGAGGGCCAAGAACACTGGGAGGGGATGGCCACAGAGCG GAGGAGGCTAGCAAAGACAAAGAAGCAGATGCTCTTCCTGCCAGCACCCAGGAACAAGACCAAGCGCACG GAAGTTCCTCACCCGAGCCAGCTGGAAGCCCTTCTGAAGGGGAGGGCGTCTCCACCTGGGAGTCATTTAA GAGATTAGTCACTCCACGAAAAAAATCCAAGTCAAAACTGGAAGAGAGAGCCGAAGACTCCGGTGCAGAG CAGTTGGCCTCCGAGATCGAACCAAGTAGAGAGGAATCTTGGGTTTCCATTAAGAAATTTATTCCTGGAC GGCGGAAGAAAAGGGCAGATGGGAAGCAAGAACAGGCCGCCGTTGAAGACTCGGGGCCAGGAGAGATCAA TGAGGACGACCCCGACGTCCCAGCTGTTGTGCCTCTGTCTGAGTACGATGCGGTAGAGAGAGAGAAGCTG GAAGCGCAGCGAGCTCAGGAGAACGTGGAGCTGCCCCAGCTGAAGGGGGCTGTGTATGTGTCTGAGGAGC TTAGTAAGACTCTGGTTCACACCGTGAGTGTCGCGGTCATTGATGGGACCAGGGCAGTCACCAGTGCCGA AGAGCGGTCCCCTTCGTGGATATCTGCTTCCATGACAGAACCTCTTGAGCACGCAGAGGGAGTGGCCACA CCGCCTGTTGGAGAGGTCACTGAAAAAGACATCACTGCAGAAGCAACTCCTGCACTCGCCCAGACTTTAC CAGGGGGCAAAGATGCCCATGACGACATAGTCACCAGTGAAGTGGATTTTACCTCAGAAGCAGTGACAGC CGCAGAAACCACAGAGGCGCTCCGCGCTGAAGAACTTACCGAAGCATCAGGGGCAGAAGAGACCACAGAC ATGGTGTCTGCAGTTTCCCAGCTGTCCGACTCCCCGGACACCACAGAGGAAGCCACCCCAGTTCAGGAGG TAGAGGGTGGCATGCTAGATACGGAAGAACAGGAGCGCCAGACGCAGGCCGTCCTCCAAGCCGTTGCAGA CAAAGTGAAAGAGGACTCCCAGGTGCCTGCAACCCAGACTCTGCAGAGAGCAGGGCCGAAAGCACTGGAG AAGGTGGAGGAGGTAGAGGAGGACTCCGAGGTGCTGGCTACCGAGAAAGAGAAGGATGTTGTGCCGGAAG GACCCGTGCAGGAAGCTGAAACTGAGCATCTTGCACAGGGCTCCGAGACTGTACAGGCTACCCCAGAGAG CCTTGAAGTTCCTGAAGTCACAGAGGATGTAGACCGTGCCACCACATGCCAGGTTATCAAGCACCAGCAG CTGATGGAACAGGCTGTGGCCCCTGAGTCATCTGAAACCTTGACAGACAGTGAGACAAATGGAAGTACTC CCCTCGCAGATTCAGACACTCCAAACGGGACACAGCAAGACGAGACCGTTGACAGCCAGGACAGTAATGC CATTGCCGCCGTCAAGCAGTCACAGGTCACTGAAGAGGAGGCAGCTGCTGCTCAGACGGAGGGGCCTTCA ACACCATCTAGTTTTCCAGCCCAGGAAGAACACAGGGAAAAACCAGGAAGGGATGTTCTAGAACCCACAC AAGCGCTGGCTGCCGGGGCAGTGCCTATTCTGGCAAAGGCTGAGGTGGGTCAAGAGGGTGAGGCTGGCCA GTTTGATGGAGAAAAAGTCAAAGACGGACAGTGTGTTAAAGAACTGGAGGTGCCTGTGCACACTGGACCC AACAGTCAAAAGACTGCTGACTTGACACGTGACAGTGAAGTAATGGAAGTGGCCAGATGTCAGGAAACTG AGAGTAATGAAGAACAGAGTATTAGCCCGGAGAAAAGAGAGATGGGAACCGACGTTGAAAAGGAGGAAAC AGAGACCAAGACAGAGCAAGCCAGTGAAGAACATGAGCAGGAAACAGCTGCTCCTGAGCATGAAGGAACC CACCCTAAGCCAGTCCTGACAGCTGACATGCCTCACTCAGAGAGGGGAAAGGCACTGGGCAGCCTTGAAG GAAGCCCTTCTCTCCCAGACCAAGACAAAGCAGATTGCATAGAGGTTCAAGTTCAAAGCTCAGACACACC AGTCACTCAAACAACCGAAGCTGTGAAAAAGGTCGAAGAAACTGTGGCAACTTCAGAGATGGATGAAAGT TTGGAGTGTGCAGGTGCGCAATCATTACCAGCTGAGAAGCTCTCCGAAACCGGTGGCTACGGGACTCTTC AGCATGGAGAGGACACCGTGCCCCAGGGGCCTGAGTCTCAGGCAGAGTCCATCCCAATAATAGTAACTCC TGCTCCTGAAAGCATCCTACATTCTGACCTTCAAAGAGAAGTGAGCGCATCCCAGAAACAGAGATCAGAT GAAGATAACAAGCCAGATGCTGGTCCTGATGCTGCCGGCAAGGAGAGTGCAGCAAGAGAGAAAATCCTCA GGGCTGAACCTGAGATCTTGGAACTTGAGAGTAAGAGCAATAAGATTGTCCAGAGTGTCATCCAGACAGC CGTCGACCAGTTTGCACGTACAGAAACAGCCCCCGAAACCCACGCTTCTGATTTACAGAATCAGGTTCCT GTGATGCAGGCTGACAGCCAGGGAGCACAACAGATGCTGGACAAAGATGAAAGCGACCTTCAAGTCTCCC CCCAAGATGGAACACTCAGTGCCGTAGCCCAGGAAGGACTTGCGGTTTCTGATAGTTCTGAAGGCATGAG CAAGGCTTCAGAAATGATCACCACGCTTGCAGTTGAAAGTGCCAGTGTCAAAGAAAGTGTTGAGAAGCTG CCTCTTCAGTGCAAAGATGAAAAGGAACATGCTGCTGACGGCCCCCAGCACCAAAGCTTAGCCAAGGCAG AGGCGGATGCCTCTGGAAATCTAACCAAAGAGTCCCCAGACACCAACGGACCAAAGCTAACCGAGGAAGG AGATGCCCTGAAAGAAGAAATGAACAAGGCCCAGACAGAAGAGGACGACCTACAGGAGCCAAAGGGAGAC CTGACAGAATCCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_031185
Insert Size	5055 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_031185.3, NP_112462.1
RefSeq Size	6262 bp
RefSeq ORF	5055 bp
Locus ID	83397
UniProt ID	Q9WTQ5
Gene Summary	Anchoring protein that mediates the subcellular compartmentation of protein kinase A (PKA) and protein kinase C (PKC).[UniProtKB/Swiss-Prot Function]

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