Mdc1 (NM_001010833) Mouse Untagged Clone

CAT#: MC224851

Mdc1 (untagged) - Mouse mediator of DNA damage checkpoint 1 (Mdc1), (10ug)



  "NM_001010833" in other vectors (2)

CNY 27,360.00


货期*
14周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 6820401C03; AA413496; mKIAA0170; NFBD; Nfbd1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC224851 representing NM_001010833
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAAGCACCCAGGTGATTGACTGGGATGCTGAAGAAGAGGAAGAGACAGAACTATCCAGTGGCTCCT
TGGGGTATAGTGTGGAGCCTATAGGGCAGCTACGTCTCTTCAGTGGAACTCATGGACCAGAAAGAGATTT
CCCCCTCTACCTTGGCAAGAATGTAGTTGGTCGAAGCCCTGACTGCTCTGTGGCCCTGCCTTTTCCATCC
ATCTCCAAACAGCATGCAGTAATTGAAATCTCAGCTTGGAACAAAGCCCCTATCCTCCAGGATTGTGGGA
GCCTCAATGGCACTCAAATCGTAAAGCCTCCTAGGGTCCTACCTCCTGGAGTGAGCCATCGTCTGAGGGA
CCAGGAATTAATTCTGTTTGCAGATTTTCCCTGCCAGTACCATCGCCTGGATGTCCCACCACCCTTGGTC
CCTCGGAGTCTTCTAACTATAGAGAAAACCCCCAGAATACGGATAGAATCCCAAAATTCCAGAGTTTTGT
TGGCTGCCGATTCAGAGGAAGAAGGGGATTTTCCTTCTGGAAGGTGTGTGGCGAATGGACAAAGGAATAC
AGCATCCCCTTCAGCAACAGTAGTTCCAGAAAGTGATGAAGAGGTCTCTTCTCCGGCCCCAAGTGTCCCC
GGGCCATCTTCACCCTTTGGTTTGGGCAGTGACACTGATGAAGAACAAGGTCAGCAACCAGGAGTAGAGG
AGTCCTCTTTAGCTGACAGCAGTGGTGCTGCAGGGGAAGCTGAGCAGCCTGAAGCTAACGGAACGACAGC
TGGCATACAGGCTCAGCCTACTGAGCACAAATTGAAGGACACAAAAGTCAAGAAGGAGGCAGGCAGGGCA
GGGGTCTCAGATGGGTCAGTTCTGGAGAGGAGCCCCACTCTTGGAGAGGACAGTGACACAGAAGTGGACG
AAGATCACAAACCTGGGTTTGCGGACAGTGAGACAGATGTGGAAGAAGAGAGGATCCCTGTGACCCCCCC
TGTAGCCCCTGTGAAGAAAAACCAAGTGCTACTTGCAGTTGGTATAGGAGATCCTGAAGCACCTGGTGTG
GCACATCTGCAGGACTGCCTAGCTGGTAGTGGCACAGATGTGGAGGACAAGACTGCACTGGATGTCCCTC
TAGAGAGAAACCACACACCCATGGTGATCAACAGTGATACAGATGAGGAGGAGGAGGAGGAGGAGGAGGA
GGTCTCAGCAGCACTCACTTTGGCCCATCTGAAAGAGAGAGGAATTGGTTTGTGGAGTAGAGACCCAGGC
GCAGAAGAGGTCAAGTCCCAACCACAGGTCCTTGTAGAACAAAGCCAGAGTGCCTCTGGGAGAGACAGTG
ACACGGATGTGGAGGAGGAATCCTCAGGGCGAAAGAGAGAGATTATCCCAGACAGTCCCATGGATGTAGA
TGAGGCTCTTACTGTCACACAGCCAGAGAGCCAGCCTCCCCGTAGGCCCAATGATGCAGATGAGTACATG
GATATGAGCTCCCCTGGCAGCCATCTAGTGGTAAATCAGGCCTCCTTTGCTGTGGTAGGCAAGACCAGAG
CACAAGTGGAGGAGGAAGTCCCAGGGCCATCTGTTATCCTGGGGGAGAAGCACCAGGTGCCTCTAGAGGG
AGCCCAGCCTCCTGAGGAAGCCTGGGAAACAGCTGTTCAGGAAGGCTCATCCTCACCAGAGGCAGCAGCC
TCTGTAAGGCCTAGCCAGCAGCCAGTAGCAGAAGATGCTGGGACAGAGTGTGCTACAGCTGTTTCTGAGC
AGGAAAGTACTCTTGAGGTGAGGTCCCAAAGCGGGTCACCTGCAGCACCAGTGGAGCAAGTGGTGATACA
TACAGATACTTCAGGGGATCCCACCCTGCCACAGAGAGAGGGAGCCCAAACCCCCACAGGAAGGGAGAGA
GAAGCACATGTGGGCAGGACCAAGAGTGCCAAAGAGTGCTGTGATGCAGAACCTGAAGATCTGTGCCTTC
CAGCTACCCAGTGCTTTGTGGAAGGGGAGAGCCAGCACCCAGAAGCTGTCCAGAGTTTGGAAAATGAGCC
TACCCAACTCTTCCCATGTACTCTTCCACAAGAGCCAGGACCTTCCCATCTCAGCCTTCAGACTCCAGGT
GCAGATACCTTGGATGTGCCTTGGGAAGTCTTGGCTACACAGCCGTTCTGTCTAAGAGAACAGTCAGAGA
CTTCTGAGCTTCATGAAGCTCATGGATCTCAACCATCTCTGCCTAGGGAACCACCAGGACACCAACATCT
GGTTCATACCAGCCCAGTTCACACAGAGCTGTTGAGAATTGAAGGCAGAGAGATACAGACTGTGGAGAAA
GCCATGGGTATACCAAAGGAAATGGCAGACAGGATGACCCCTGAGAGAGAGCCATTGGAGAGGGAAATCA
GGGGAAGAACAGAAAACAGTGAGAGAGATGTGATAGGAGAAGAACTAATTCAGGGGACAAAGGACAGAGA
GCCAAAGAAGGTGTTAGCTAGAGACAGTCAGAGAAAAGAGGCTGACAAGGATCTTGAAGGGAATAGGGAG
AGTTTGGAGGTAGAAATAGAGATGTCCAAAGACTCACAGAAGAGAGAGAGAAAAGTAGAGAAACCAGAAC
CAAAGAGAGAATGGGAGCCAGCTGATTTAGAAGTGACTCCAGATAGAGGGGTGACAGAGGAAGGGAGCCA
TGATCAGAAAGGGCAAATTGCTAGTCTAACACTAAAGCCTGGAGTTGGGGTGAAGGACCTTGAGGGACTT
GCATCAGCCCCAATAATCACAGGGAGCCAGGCAGATGGAGGAAAGGGAGACCCTTTGAGCCCTGGGAGGC
AGCAGAGAGGCCGCTTGAGTTGCCAGACGACACCTGCTGGAAAGGCTTCCAGGGGTGATCCAGAGCCCCC
AGACCACTGCCTGTTTTCTTCAGTGCCTGAAGCTTCAACTCAGAGTCTCCTCACCTCTCAGAGCCAAAAG
CAGTCTACACCTCAGCCTCTGTTTTCGACCTCTTCTTCTGAGATACCCCTTCCTGAGAGTCTTCACACAA
AGCCTAATGTCAGGCCACGGCGGTCCTCCAGGATGACCCCCTCCCCGCATTCCTCTGCTGCCCTTAAGCC
AAATACTACCTGCCCCACAAACCAGCCTGCTGCCTCTAGACCAACATCCCGACCCACTCGGGGCAGGGCA
AATAGGTCCTCTACCAGGACCCCAGAACTGATTGTCCCTGTAGACCCTGAACTCCAGCCTTCCACCTCCA
CAGAACAGCCTGTCATCCCAAAACTTACGTCCCAGGTCACTGAGGGCAGGGTACAGATGCCTGAACCACT
TCTCACAGGTCCCGAGATCCAGTCTCCCACCTCCACAGAACAGTCTGTCACCCCAGACCGCAAACCTCGG
GCCACTCGGGGCAGGCCAAGTAAATCTCCCAACAAGACCCCAGAACCACTTATTTCTACTGGCCCTGAAC
TCCAACCTCCCACCTCCATAGAACAACCTGTCATACCTAAACCCACTTCTCGGGTCACTCGGGGCAGACC
ACGTAAGTCTTCTGTCAGGACCCCAGAATCAGTTGTCTCCACTGGCCCTGAACTTCAGCCTCTCACCTCT
ATAGAACAGCCTGTTATCCCTGAACCTAGGGCCACTCGAGGTAGACCAAGTAAGTCTTCTATCAAGACCC
CAGAATCAGTTGTCCCCACTGGTCCTGAGCTGCAGCCTCTTACCTCTGCAAAACAGCCTGTCACCCCTAA
CCTCACATCTCGGGCCTCTCGGGGTAGATCAAGTAAGTCTATCAGGACCCCGGAACCAGTTGTCCAAACT
GGCCCTGAATTCCATCCATCCACCTCCACAGAACAGCCTGATACCCGTGAGCCCTCATCTCAGGCTAGGA
CACGTAGGTCTGCTGTCAAGACTCCTGAAGCGAGTGTCCCCACAACCCCTGAACTCCAGCCTTTCACTTC
TAAAAAACAGCCTGCCCCTAAGCCTACAGCTCTGGTCACTCAGGGAAGGACATATAAACCCTCCACTGAA
GATTGTGAATCAGTTGGGCCAGTGGCCCCTGATTTTGAGCCTTCCACCTCTACAGACCATCTTGTCACCC
CTAAGGTTACAGATCAGAGCTTAACACTACAGTCTTCACCCCTAAGTGCTTCCCCAGTTTCCAGTACCCC
TGATCTCAAGCCTCCTGTCCCCATAGCCCAGCCTGTTACCCCAGAGCCCATTCCACAAGCCAATCACCAA
AGGAAACGAAGGGCTGCTGGGAAGCAGGGTTCCCGCACAGTCCCTCTGGGTCATAAGTCTTACTCTGCCC
TCTCTGAACCTGAGCCCCAATCTTCAGCCAGCCAAAGCTCAGGAGCATCAGAAGCAGACTCACCCCGTCA
AAAACGTCCCAGAAGACAAGCCTCCCAGAAAACTGTAGTCATCAAGGAAGAACCTGTAGAAACAGAAGTG
AAGGAAGAGCCTCAAGAGACTGCAATTCCAACACCAGAAAAGAGAAAGAGGGACCATGCAGAAGAAGTGA
CCCAGGGAAAACCAACTCGAAGCCGGCGAACTAAACCTAACCAAGAAACAGCCCCCAAGGTACTGTTCAC
AGGAGTCATGGATTCTCGTGGCGAGCGTGCAGTGCTGGCTCTGGGAGGCAGTCTGGCCAGCTCAGTAAAT
GAGGCCTCCCACTTAGTTACTGATCGCATCCGCAGGACAGTCAAGTTCTTGTGTGCCCTGGGGAAGGGAA
TTCCCATCCTGTCCCTGAACTGGCTGTACCAGTCCCGAAAGGCTGGTTGCTTCTTGCCGCCTGATGACTA
CTTAGTGACTGATCCTGAGCAAGAGAAGAACTTTAGCTTCAGCCTTCGGGATTCCCTGTGCCGGGCTCGG
GAACGAAGACTGCTGGAGGACTATGAGATTCATGTGACCCCTGGCGTGCAGCCACCCCCCCCTCAGATGG
GCGAGATCATCAGTTGCTGTGGAGGCACTTTCCTGCCCAGCATGCCTCACTCCTATAAGCTTCACCGAGT
CATCATAACTTGCACTGAAGACCTACCACGCTGTGCTATTCCATCCCGCCTGGGGCTACCCCTCCTCTCT
CCTGAGTTCCTCCTGACTGGAGTGCTGAAGCAGGAAGCCACACCAGAGGCCTTTGTTCTCTCCAATTTGG
AAATGTCATCTACCTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001010833
Insert Size 5127 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001010833.2, NP_001010833.2
RefSeq Size 7372 bp
RefSeq ORF 5127 bp
Locus ID 240087
UniProt ID Q5PSV9
Gene Summary The protein encoded by this gene contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 7 divergent copies of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci. Mice with mutations in this gene exhibit growth retardation, male infertility, immune defects, chromosome instability, DNA repair defects, and radiation sensitivity. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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