Shank3 (NM_021423) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI841104; Shank3b; SHANK3c-3; SHANK3c-4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224879 representing NM_021423 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGGCCCCGGGGCCAGCGCCGTGGTCGTGCGCGTCGGCATCCCGGACCTGCAACAAACGAAGTGCC TGCGTCTGGACCCAACCGCGCCCGTGTGGGCCGCCAAGCAGCGTGTGCTCTGCGCCCTCAATCATAGCCT TCAAGACGCGCTCAACTACGGGCTATTCCAGCCTCCCTCCCGGGGTCGCGCCGGCAAGTTCCTGGATGAA GAGCGGCTCTTACAGGACTACCCGCCTAACCTGGACACGCCCCTGCCCTATCTGGAGTTCCGATACAAGC GGAGAGTTTATGCCCAGAACCTCATAGATGACAAGCAGTTTGCAAAGCTACACACAAAGGCAAACCTGAA GAAGTTCATGGACTATGTCCAGCTACACAGCACAGATAAGGTGGCCCGCCTGCTGGACAAGGGGCTGGAC CCCAATTTCCATGACCCTGACTCAGGAGAGTGCCCTCTGAGCCTTGCGGCACAGTTGGACAACGCCACTG ACCTCCTGAAGGTTCTCCGCAACGGCGGTGCTCATCTGGACTTCCGGACCCGAGATGGGCTGACAGCCGT CCACTGTGCTACCCGCCAGCGGAACGCAGGGGCATTGACGACCCTGCTGGACCTGGGGGCTTCGCCTGAC TACAAGGACAGCCGCGGCCTGACGCCCCTGTACCATAGTGCCCTAGGGGGCGGGGATGCCCTCTGTTGCG AGCTGCTTCTCCATGATCATGCACAGCTGGGGACCACTGATGAGAATGGTTGGCAAGAGATCCATCAGGC CTGTCGCTTTGGACACGTGCAGCACCTGGAGCACCTTTTGTTCTATGGGGCCAACATGGGTGCTCAGAAT GCCTCGGGAAACACAGCCCTGCACATCTGTGCCCTCTACAACCAGGAGAGTTGCGCGCGCGTCCTGCTTT TCCGTGGTGCCAACAAGGACGTCCGCAATTACAACAGCCAGACAGCCTTCCAGGTGGCCATTATTGCAGG GAACTTTGAGCTTGCCGAGGTAATCAAGACCCACAAAGACTCCGATGTCGTACCATTCAGGGAAACCCCC AGCTATGCAAAGCGACGGCGTCTGGCTGGCCCGAGTGGCCTGGCATCCCCACGGCCCTTACAGCGCTCAG CCAGTGATATCAACCTGAAAGGTGATCAGCCCGCAGCTTCTCCAGGGCCCACTCTCCGAAGCCTCCCTCA TCAACTCTTGCTCCAGAGGCTTCAGGAGGAGAAAGACCGTGACAGGGATGGTGAACTGGAGAATGACATC AGCGGCCCCTCAGCAGGCAGGGGTGGCCACAACAAGATCAGCCCCAGTGGGCCCGGCGGATCCGGCCCCG CGCCCGGCCCCGGCCCGGCGTCTCCCGCGCCCCCCGCGCCGCCGCCCCGGGGCCCGAAGCGGAAACTTTA CAGTGCCGTCCCCGGCCGCAAGTTCATCGCTGTGAAGGCGCACAGCCCGCAGGGCGAGGGCGAGATCCCG CTGCACCGCGGCGAGGCCGTGAAGGTGCTCAGCATTGGGGAGGGCGGTTTCTGGGAGGGAACCGTGAAGG GCCGAACAGGCTGGTTCCCAGCTGACTGTGTGGAAGAAGTGCAGATGCGACAGTATGACACCCGGCATGA AACCAGAGAGGACCGGACGAAGCGTCTCTTCCGCCACTACACTGTGGGTTCCTATGACAGCCTCACTTCA CACAGCGATTATGTCATCGATGATAAGGTGGCTATCCTGCAGAAAAGGGACCATGAGGGGTTTGGCTTTG TTCTCCGGGGAGCCAAAGCAGAGACCCCCATTGAGGAGTTTACACCCACACCTGCCTTCCCTGCACTCCA ATACCTTGAGTCTGTAGATGTGGAAGGTGTGGCCTGGAGGGCTGGACTTCGAACTGGGGACTTCCTCATT GAGGTGAACGGAGTGAATGTCGTGAAGGTTGGACACAAGCAAGTGGTGGGTCTCATCCGTCAGGGTGGCA ACCGCCTGGTCATGAAGGTTGTGTCTGTGACCAGGAAACCCGAGGAGGATGGTGCTCGGCGCAGAGCCCC ACCACCCCCAAAGAGGGCTCCCAGCACCACGCTGACCCTGCGGTCCAAGTCCATGACGGCTGAGCTCGAG GAACTTGCTTCCATTCGGAGAAGAAAAGGGGAGAAGTTGGATGAGATCCTGGCAGTTGCCGCGGAGCCGA CACTGAGGCCGGACATTGCAGATGCTGACTCGAGGGCGGCCACTGTCAAGCAGCGGCCCACCAGCCGGAG GATCACCCCTGCTGAGATCAGCTCATTGTTTGAGCGCCAGGGCCTCCCAGGCCCAGAGAAGCTGCCGGGC TCTCTGCGGAAGGGGATTCCACGGACCAAATCTGTAGGGGAGGATGAGAAGCTGGCATCCCTACTGGAAG GGCGCTTCCCACGCAGCACGTCAATGCAGGACACAGTGCGTGAAGGTCGAGGCATTCCACCCCCGCCGCA GACCGCCCCGCCACCCCCACCCGCGCCCTACTACTTCGACTCCGGGCCACCCCCCACCTTCTCACCGCCG CCACCACCGGGCCGGGCCTATGACACTGTGCGCTCCAGCTTCAAACCAGGCCTGGAGGCTCGTCTGGGTG CGGGGGCCGCCGGCCTGTATGATCCGAGCACGCCTCTGGGCCCGCTGCCCTACCCTGAGCGTCAGAAGCG TGCGCGCTCCATGATCATACTGCAGGACTCTGCGCCAGAAGTGGGTGATGTCCCCCGGCCTGCGCCTGCC GCCACACCGCCTGAGCGCCCCAAGCGCCGGCCTCGGCCGTCAGGCCCTGATAGTCCCTATGCCAACCTGG GCGCCTTCAGTGCCAGCCTCTTCGCTCCGTCGAAACCACAGCGCCGCAAGAGCCCGCTGGTGAAGCAGCT TCAGGTGGAGGACGCTCAGGAGCGCGCGGCCCTGGCCGTGGGTAGCCCGGGACCAGTGGGCGGAAGCTTT GCACGAGAACCCTCTCCGACGCACCGCGGGCCCCGGCCAGGCAGCCTTGACTACAGCTCTGGAGAAGGCC TAGGCCTTACCTTCGGCGGCCCTAGCCCTGGCCCAGTCAAGGAGCGGCGCCTGGAGGAGCGACGCCGTTC CACTGTGTTCCTCTCTGTGGGTGCCATCGAGGGCAGCCCTCCCAGCGCGGATCTGCCATCCCTACAACCC TCCCGCTCCATTGATGAGCGCCTCCTGGGGACAGGCGCCACCACTGGCCGCGATTTGCTACTCCCCTCCC CTGTCTCTGCTCTGAAGCCATTGGTCGGTGGTCCCAGCCTTGGGCCCTCAGGCTCCACCTTCATCCACCC TCTCACTGGCAAACCCTTGGATCCTAGCTCACCCTTAGCTCTTGCTCTGGCTGCCCGGGAGCGGGCTCTG GCCTCGCAAACACCTTCCCGGTCCCCCACACCTGTGCACAGCCCCGATGCTGACCGCCCTGGACCCCTCT TTGTGGATGTGCAAACCCGAGACTCTGAGAGAGGACCGTTGGCTTCCCCAGCCTTCTCCCCTCGGAGTCC AGCGTGGATTCCAGTGCCTGCTCGGAGAGAGGCAGAGAAGCCCCCTCGGGAAGAGCGGAAGTCACCAGAG GACAAGAAGTCCATGATCCTCAGCGTCTTGGACACGTCCTTGCAACGGCCAGCTGGCCTCATTGTTGTGC ATGCCACCAGCAATGGGCAGGAGCCCAGCAGGCTGGGGGCTGAAGAGGAGCGCCCCGGTACTCCGGAGCT GGCCCCAGCCCCCATGCAGGCAGCAGCTGTGGCAGAGCCCATGCCAAGCCCCCGGGCCCAGCCCCCTGGC AGCATCCCAGCAGATCCCGGGCCAGGTCAAGGCAGCTCAGAGGAGGAGCCAGAGCTGGTATTCGCTGTGA ACCTGCCACCTGCTCAGCTGTCCTCCAGCGATGAGGAGACCAGAGAGGAGCTGGCCCGCATAGGGCTAGT GCCACCCCCTGAAGAGTTTGCCAATGGGATCCTGCTGACCACCCCGCCCCCAGGGCCGGGCCCCTTGCCC ACCACGGTACCCAGCCCGGCCTCAGGGAAGCCCAGCAGCGAGCTGCCCCCTGCCCCTGAGTCTGCAGCTG ACTCTGGAGTAGAGGAGGCTGACACTCGAAGCTCCAGTGACCCCCACCTGGAGACCACAAGCACCATTTC CACAGTGTCCAGCATGTCCACCCTGAGCTCGGAGAGTGGGGAACTCACGGACACCCACACCTCCTTTGCC GATGGACACACTTTTCTACTCGAGAAGCCACCAGTGCCTCCCAAGCCCAAGCTCAAGTCCCCGCTGGGGA AGGGGCCGGTGACCTTCAGGGACCCGCTGCTGAAGCAATCCTCGGACAGTGAGCTCATGGCCCAGCAGCA CCATGCTGCCTCTACTGGGTTGGCTTCTGCTGCTGGGCCCGCCCGCCCTCGCTACCTCTTCCAGAGAAGG TCCAAGCTGTGGGGGGACCCCGTGGAGAGTCGGGGGCTCCCTGGGCCTGAAGATGACAAACCAACTGTGA TCAGTGAGCTCAGCTCCCGTCTGCAGCAGCTGAATAAAGACACACGCTCCTTGGGGGAGGAACCAGTTGG TGGCCTGGGCAGCCTGCTGGACCCTGCTAAGAAGTCACCCATTGCAGCAGCTCGGCTCTTCAGCAGCCTC GGTGAGCTGAGCACCATCTCAGCGCAGCGCAGCCCGGGGGGCCCGGGCGGAGGGGCCTCCTACTCGGTGC GGCCCAGCGGCCGGTACCCCGTGGCGAGACGAGCCCCGAGCCCAGTGAAACCCGCATCGCTGGAGCGGGT GGAGGGGCTGGGGGCGGGCGTGGGAGGCGCGGGGCGGCCCTTCGGCCTCACGCCTCCCACCATCCTCAAG TCGTCCAGCCTCTCCATCCCGCACGAACCCAAGGAAGTGCGCTTCGTGGTGCGAAGTGTGAGTGCGCGCA GCCGCTCCCCCTCACCATCTCCGCTGCCCTCGCCTTCTCCCGGCTCTGGCCCCAGTGCCGGCCCGCGTCG GCCATTTCAACAGAAGCCCCTGCAGCTCTGGAGCAAGTTCGATGTGGGCGACTGGCTGGAGAGCATCCAC TTAGGCGAGCACCGAGACCGCTTCGAGGACCATGAGATCGAAGGCGCACACCTGCCTGCGCTCACCAAGG AAGACTTCGTGGAGCTGGGCGTCACACGCGTTGGCCACCGCATGAACATCGAGCGTGCGCTCAGGCAGCT GGATGGCAGCTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_021423
Insert Size	5193 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_021423.3, NP_067398.2
RefSeq Size	7131 bp
RefSeq ORF	5193 bp
Locus ID	58234
UniProt ID	Q4ACU6
Gene Summary	Major scaffold postsynaptic density protein which interacts with multiple proteins and complexes to orchestrate the dendritic spine and synapse formation, maturation and maintenance. Interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and HOMER, respectively, and the actin-based cytoskeleton. Plays a role in the structural and functional organization of the dendritic spine and synaptic junction through the interaction with Arp2/3 and WAVE1 complex as well as the promotion of the F-actin clusters. By way of this control of actin dynamics, participates in the regulation of developing neurons growth cone motility and the NMDA receptor-signaling. Also modulates GRIA1 exocytosis and GRM5/MGLUR5 expression and signaling to control the AMPA and metabotropic glutamate receptor-mediated synaptic transmission and plasticity. May be required at an early stage of synapse formation and be inhibited by IGF1 to promote synapse maturation.[UniProtKB/Swiss-Prot Function]

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