Cep152 (NM_001081091) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI851464; mKIAA0912
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224893 representing NM_001081091 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCATTGGAGTTTGGCAGTGTAGCACTGCAGACACAAAATGAAGATGAAGAATTTGACAAAGAAGACT TTGAAAGGGAAAAAGAGTTGCAGCAGCTACTCACAGACCTCCCTCACGACATGCTGGACGATGAGCTCTC CTCTCCTGAGCGCCACGATTCTGACTGCAGTATGGACGGCAGAGCGGCAGAACCGCATCCTTCTGAGCAT CTGGAAAGGAAGTGGATTGAGCGGGATATACTGCCCAAGCCTCACAGCATGAACTGTGGCAATGGCTGGG AAGAAAATCGAAGTAAAACCGAAGACCAGCATCTTGGGTACCACCCTGGAGAAGGTGGGGATGAAGGCGG AAGTGGCTATAGCCCTCCAGGTAAACGGGAGCAGGCAGATTTGTATCGCCTTCCTGAAGACTTTAGGCCA TACACCGGCGGTTCGAAGCAGGCAGCCAGCGTGATTACGTTTTCAGATCCTCAGAGGGACAATTTTCAGC AATTTGGTTTATCTCGAGGACCCAGCTGTGGAGCTTTGGAGCCGTATAAAGCTGTATATAAGCCTTACCG TAACTCCTCTGTGCAGAAGAACAGCTCGCCAGCCCAGGAGGTAGCAGCAAGTGACATGTTTGAAGGCTTG CAGCAACAGTTTTTAGGAGCTAATGAAACAGACTCTGCAGAAAATATACATATTATTCAACTCCAGGTTC TTAACAAAGCAAAGGAGAGACAACTGGACAGCTTGGTTGAAAAGCTGAAGGACAGCGAACGCCAGGTTCG GTATCTGAGCCACCAGCTGCTGATAGTCCAAGATGAAAAGGATGGCTTGGCCCTCAGCCTTCGGGAATCC CAGCAGCTCTTTCAGAATGGCAAGGAGAGGGAGATGCAGCTTGAAGCTCAGATTGCAGCACTGGAGGCGC AGGTCGAGGCCTTCCGAGTTAGTGAAGAGAAGCTCACCAAGAAGCTCAGAACAACCGAGATCACCCTGGA AAGCCTGAAACAGCAACTGGTGGAGCTCCATCATTCTGAGTCACTTCAGCGAGCCAGAGAGCACCATGAA AGCATCGTTGCCAGCCTCACACAGAAGCACGAAGAGCAGGTGTCATCCTTACAGAAGAATTTGGACGCTA CCATAACGGCCCTTCAGGAACAGGAAAGTATTTGCACGCGTTTGAAGGACCACGTGCAACAACTGGAGCG GAACCAGGAAGCAGTCAGATTAGAGAAGACGGAGCTCATTAATAGGCTGACGAGGAGTCTAGAGGACAGT CAGAAGCAATGTGCCCACCTGCTGCAATCAGGCTCTGTGCAGGAGGTGGCTCAGCTACAGCTACAGTTGC AGCAAGCACAGAAGGCCCACGTCCTAAGTGAGAGCATGAATAAGGCTTTGCAAGAGGAATTAACAGAGCT TAAAGATGAGATTTCGCTCTATGAATCTGCTGCAGAACTCGGAGTCCTTCCAGGTGACTCCGAAGGAGAC CTGAGTATAGAGCTCACTGAATCATGTGTGGACTTGGGCATTAAAAAAGTCAATTGGAAACAATCCAAAG CTAACAGGGTCACACAGCAGGAGTCCCCAGATGAAGACCCTTCGAAGGATGAGCTTATCCTGAAGCTGAA AACGCAAGTCCAGCGCTTGCTGACTAGCAACTCCGTGAAGCGACATCTGGTGTCTCAGCTGCAGAGCGAT CTCAGAGAGTGCCGGGAGACAATGGAAGCTTTCCAGCAATCCAAGGATGGTGACAGCGGCATGGAGACTA AAACAGACACCTCTGAAAAAACAACTAAGCAGTTATGGCTCGAATCTTCTGAGGCAATTAACAGAGAAGA TATTCTGCAACTGAAAAATGAAGTTCAAGTTTTACAAAAACAGAATCAGGAACTGAAAGAGGCTGAAGAA AAACTGAGAAGTACAAATCAAGACTTGTGTAATCAGATGAGACAGATGGTGCAGGAGTTTGACCACGATA AGCAGGAAGCTGTGGCTAGGTGTGAGAGGACTTACCAGCAGCACCACGAAGCCATGAAGGCGCAGATCCG AGAGAGCCTTCTAGCAAAGCACGCTGTGGAGAAGCAGCATCTCTTGGAGGTCTATGAGGGGACCCAGTCA CAGCTCAGGTCTGACCTGGATAAGATGAACAAGGAGATGGCTGCTGTGCAGGAGTGTTACCTGGAGGTAT GCAGAGAGAAGGATGGGCTGGAGTCCACTCTCAGGAAGACCATGGAAAAGGCTCAGGAGCAGAAGAGACA GCTTTTGGAAGCCAGAGAAGAATACGTGCGCAAGCTGAAGTTAGAACTGGAAGAGAAGTACCAAGAAACT CTGAAGACGGAAAGGCAGAGCTGGCTGCAGGAGCAAGCTGCTGGAGCTACACAGCAGGCTGAGAAGGAGA GCAGACAAAAACTCATTCAACAGCTGGAAAAGGAGTGGCAGTCCAAACTGGATGACAGTCTTGCGGCTTG GAGAAAGACCACCTCAGACCGCGGCAGCCAGACCGAGCAGGTGGCCTGTCCTGCTGCCGTGTCTAAGGCA GAGGCAGCAGCAGTCCTGGCAGAGGAGCAGGCTCGCCAAGTCCAGCAGGAGAAGGAGCTTGCTACCAAGG AGGCTCTGAGGAAGCCCGAAGTTGAGTTGGAGCTCAAGTACTGTGAGATTATTGCACAAAAGGTGGAAAC AGCTGTGCAGAATGCCCGAAGTCGCTGGATACAGGAGCTGCCCATGCTTGCAGAGTACAAGGCTCTGCTC AGAGCCCAGCAGCAGGAGTGGGCAAAGCAGCAGGAGCTGGCTGTGGCCCATAGGCTGTCACTCGCACTCT CTGAAGCTAAAGAGAAGTGGAAAAGTGAGCTGGAAAATATGAAGCCAAATGTCATGTCTGTCAAGGAGCT GGAGGAGAAAGTCCACTCTCTGCAGAAGGAGCTGGAGTTGAAGGATGAGGAAGTCCCAGTGATTGTCAGA GCTGAGGTGGCTAAGGCCAGAACTGAGTGGAACAAAGAAAAACAGGAAGAAATCCACAAGATTCAAGAAC AAAACGAGGAGGATTATCGGCAGTTTTTAGAGGATCATCGAAATAAAATTAATGAGGTGCTTGCAGCAGC TAAAGAGGACTTTGTGAAGCAAAAAGCTGAACTACTTCTTCAGAAGGAGACAGAATTCCAGGCATGTTTG GACCAGAGTCGCAAGGAGTGGACACTGCAGGAAGCCCAGCAGACACAAGTGGAAATCCGTCAGTATGAGG AAGACACCCTCACTGTACTTGCCTATCTTCTAAAGGACACGCAGCTGGAGTACGGTGGGGACTCACAGGA CAAGCAGCTTTTGGAAGCCATGTCAGCTTGTTCTTCCAAATGGATATCTGTACAGTACTTTGAGAAAGTA AAAGCCTGCATACAAAAAGCTCTTCACGATATGCTGTCCTTGCTTACAGACAGTGTCGCCTCAGAGCAGG AGAAGAGAAAGGTGGTCAAGAGCTCTGCAGACACTGTCAGCTGGACCAGTAGCGAAGGAGACTCTGCGGT CCCAGTGCCCCTTCCTGACTCCACCTCTGTACGCTGTGCTCAGTCCTCGGCCTGGCTGAAAGCAGAAGCA GAAACTGATAAAAAGATCTGTGAAATTAAAGGTTTACGCTGTGGACACTGCTTTCAAGAGCTTGAAAAGG AAAAGCAGGAGTGTCAAGATCTGAGAAGGAAACTGGAGAAGTGCCGTAGGCACCTTCAGCATTTAGAAAG GACACACAGAGCCGCAGTGGAGAAACTTGGAGAGGAGAACAGCAGGGTGGTTGAAGAATTAATAGAGGAG AACCATGACATGAAGAATAAACTGGAGGCACTGAGGGCCCTTTGCAGAACGCCACCACGGTCACTGTCTG CAGGAGCCGCTGAGAGTGCTGGGCCGTCATGCAGTAGGCAGGCCTTGGAAGAACTCCGCGGGCAGTACAT TAAAGCTGTGAGAAAAATTAAACGTGACATGCTTCGGTATATTCAGGAGAGCAAGGAAAGAGCTGCAGAG ATGGTAAAAGCGGAAGTGTTGCGGGAACGGCAGGAAACTGCACGGAAGATGCGCAATTATTACCTGAGTT GTCTTCAGCAGATCTTGCAGGATAATGGAAAAGAGGAGGGAGCTGAGAAAAAGATTATGAGTGCTGCTAG CAAACTCGCTACAATGGCGGAGTTGCTGGGGACGATCGCGGAGTCAGACTGCAGAGTCCGGTGTGCCCAG GCAGGCCGCTCAGTAGCACTTCCTTTGGCTTCAGAGATGCTGACGGGCACTGAAAGATCAGAAAGGAGTG GTGTGAATCACAATATTCCACATTACGTTGAAAGCAAACCAAACAGTGGAAAGACCCTCCCCAGGAGTGT GTGTGAGCAGCTGCCTGGGAGGAAGGCTGCGCCCAGGTCACAGAGGCGGTTAGAGGAATCGAAGCACAGA GAGATGAGACCCATGGCTTCCACAGCTTTGCCTTCAGACTGTCGCTGTGGGGATGCGAGTTGCAGGCACT CAGGCGTCTTGGCAAAGGATGTTGCTCCTGAATTTGTTCCGTGCCAGGGCGAGGGAGGCTTTGACTTGCA CGAGAAGAGAGATGCACTTGGAGCTGGCTCTGAGCCGCTGCTCTATTCAGCTGCTCACTCCTTCCTTGGA GGTGCCGAAAAGAACTCTTCTCCTAGATGCATCTCAGAGTCCAGGCACACAACCCTGAGAAGTCCCAGTG AAATGCCAAGGTTAAAGGCATTGATGTGTGGTTCTCCTACAGAAACTGACAGTATTGCATCTGAGAAGAG TCAGGGAGTGGGCTCTCAGGACTCCCCAGTGAAGGATGGCGTGGGGCCCAGCAGCTCTCCAGCCTGGCCT TCTGACAGTACCTTACCCTGTGGCAGTCCTGCAGTGCTGTTTCTTGGGGATGGGTCACAAAGGACTCAGG AGATGCTGGGGGACTCTGTTCAGTGGAAACAATTCTCAGCAACCAGTTGTCATCCAGATGCCCAGAAAAG TAATATGGTTTGTCGGAGTAGTCACACTCTAGATCTGCCCAAAGAAACCCTGCATTCCCAACAAGGGAAG ATGGGTGCAACTCTGGGACACCCATCTCCACAGAGCACTGATATGTTAAAGACAGATTTCAAGAGACTCA GTGGCACAGGACCATCTTCATTGTGTCAGAAGCCTTTGATAAAGTTAACTGCTCCGATGCCCAGCCAACA GGATAGTGGCTTTGACAGCCCGCTTGAGTAA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001081091
Insert Size	5211 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001081091.1, NP_001074560.1
RefSeq Size	5768 bp
RefSeq ORF	5211 bp
Locus ID	99100
UniProt ID	A2AUM9
Gene Summary	Necessary for centrosome duplication; the function seems also to involve CEP63, CDK5RAP2 and WDR62 through a stepwise assembled complex at the centrosome that recruits CDK2 required for centriole duplication (By similarity). Acts as a molecular scaffold facilitating the interaction of PLK4 and CENPJ, 2 molecules involved in centriole formation (By similarity). Proposed to snatch PLK4 away from PLK4:CEP92 complexes in early G1 daughter centriole and to reposition PLK4 at the outer boundary of a newly forming CEP152 ring structure (By similarity). Also plays a key role in deuterosome-mediated centriole amplification in multiciliated that can generate more than 100 centrioles (PubMed:24240477). Overexpression of cep152 can drive amplification of centrioles.[UniProtKB/Swiss-Prot Function]

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