Itgb4 (NM_133663) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AA407042; C230078O20; CD104
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224908 representing NM_133663 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGGGCCCTGTTGCAGCCCATGGGTGAAGCTGCTGCTGCTGGCAGCAATGCTGAGTGCCAGCCTCC CTGGAGACCTGGCCAACCGCTGCAAGAAGGCTCAGGTGAAGAGCTGTACCGAGTGCATCCGGGTGGACAA GAGCTGTGCCTACTGCACAGACGAGCTGTTCAAGGAGAGGCGCTGCAACACCCAGGCGGAGCTTCTGGCT GCAGGCTGCAGGGGAGAGAGCATCCTGGTCATGGAGAGCAGCCTTGAAATCACAGAGAACACCCAGATCG ACACCAGCCTGCACCGCAGCCAGGTATCTCCCCAAGGCCTGCAAGTCCGGCTGCGGCCGGGTGAGGAGCG CAGCTTTGTGTTCCAGGTGTTTGAGCCCCTGGAGAGCCCCGTGGATCTGTATATCCTCATGGACTTCTCC AACTCCATGTCTGACGATCTGGACAACCTCAAGCAGATGGGGCAGAACCTGGCCAAGATCCTGCGCCAGC TCACCAGCGACTACACCATTGGATTTGGAAAGTTTGTGGACAAAGTCAGCGTCCCACAGACAGACATGAG GCCCGAGAAACTGAAGGAGCCCTGGCCCAACAGTGATCCCCCGTTCTCCTTCAAGAACGTTATCAGCTTA ACGGAGAATGTGGAAGAATTCTGGAACAAACTGCAAGGAGAACGCATCTCAGGCAACCTGGACGCTCCTG AAGGGGGCTTTGATGCCATCCTGCAGACAGCTGTGTGCACAAGGGACATTGGCTGGAGGGCTGACAGCAC CCACCTGCTGGTGTTCTCCACCGAGTCTGCCTTCCACTACGAGGCTGATGGTGCCAACGTGCTGGCCGGC ATCATGAACCGCAATGATGAGAAATGCCACCTGGACGCCTCGGGCGCCTACACCCAATACAAGACACAGG ACTACCCATCAGTGCCCACGCTGGTTCGCCTGCTTGCCAAGCATAACATCATCCCCATCTTTGCTGTCAC CAACTACTCTTACAGCTACTATGAGAAGCTCCATAAGTATTTCCCCGTCTCCTCTCTGGGCGTCCTGCAG GAGGATTCATCCAACATCGTGGAGCTGCTGGAGGAGGCCTTCTATCGAATTCGCTCCAACCTGGACATCC GGGCTCTGGACAGCCCCAGAGGCCTGAGAACAGAGGTCACCTCCGATACTCTCCAGAAGACGGAGACTGG GTCCTTTCACATCAAGCGGGGGGAAGTGGGCACATACAATGTGCATCTCCGGGCAGTGGAGGACATAGAT GGGACACATGTGTGCCAGCTGGCTAAAGAAGACCAAGGGGGCAACATCCACCTGAAACCCTCCTTCTCTG ATGGCCTCCGGATGGACGCGAGTGTGATCTGTGACGTGTGCCCCTGTGAGCTGCAAAAGGAAGTTCGATC AGCTCGCTGTCACTTCAGAGGAGACTTCATGTGTGGACACTGTGTGTGCAATGAGGGCTGGAGTGGCAAA ACCTGCAACTGCTCCACCGGCTCTCTGAGTGACACACAGCCCTGCCTGCGTGAGGGTGAGGACAAACCGT GCTCGGGCCACGGCGAGTGCCAGTGCGGACGCTGTGTGTGCTATGGTGAAGGCCGCTACGAGGGTCACTT CTGCGAGTATGACAACTTCCAGTGTCCCCGGACCTCTGGATTCCTGTGCAATGACCGGGGTCGCTGTTCT ATGGGAGAGTGTGTGTGTGAGCCTGGTTGGACAGGCCGCAGCTGTGACTGTCCCCTCAGCAATGCCACCT GCATCGATAGCAACGGGGGCATCTGCAACGGCCGAGGCTACTGTGAGTGTGGCCGTTGTCACTGCAACCA GCAGTCGCTCTACACGGACACCACCTGTGAGATCAACTACTCTGCGATACGCCTGGGTCTCTGTGAGGAT CTCCGCTCCTGCGTACAGTGCCAGGCCTGGGGCACCGGGGAGAAGAAAGGGCGCGCGTGTGACGATTGCC CCTTTAAAGTCAAGATGGTAGACGAGCTTAAGAAAGAAGAGGTGGTGGAGTACTGCTCCTTCCGGGATGA GGATGACGACTGCACTTACAGCTACAACGTGGAGGGCGATGGCAGCCCTGGGCCCAACAGCACAGTCCTG GTCCACAAAAAGAAAGACTGCCCTCCCGGCTCCTTCTGGTGGCTCATCCCCCTGCTCATCTTCCTCCTGT TGCTCCTGGCGTTGCTTCTGCTGCTCTGCTGGAAATACTGTGCCTGCTGCAAAGCCTGCCTGGGGCTTCT TCCTTGCTGCAACCGAGGTCACATGGTGGGCTTTAAGGAAGATCACTATATGCTTCGGGAGAACCTGATG GCCTCTGACCACCTGGACACGCCCATGCTACGAAGCGGGAACCTCAAGGGACGAGACACAGTCCGCTGGA AGATCACCAACAATGTGCAGCGCCCTGGCTTTGCCACCCATGCCGCCAGCACCAGCCCCACGGAGCTCGT ACCCTACGGGCTGTCCCTGCGCCTTGGCCGCCTCTGCACTGAGAACCTTATGAAGCCGGGCACCCGAGAG TGTGACCAGCTACGCCAGGAGGTGGAGGAAAATCTGAATGAGGTGTATAGACAGGTCAGCGGCGCACACA AGCTCCAGCAGACGAAGTTCCGACAGCAGCCCAACACCGGGAAAAAGCAAGACCACACCATTGTGGACAC AGTGTTGCTGGCGCCCCGCTCCGCCAAGCAGATGCTGCTGAAGCTGACAGAGAAGCAGGTGGAGCAGGGG TCCTTCCATGAACTGAAGGTGGCCCCTGGCTACTACACTGTCACGGCAGAGCAGGATGCCCGGGGCATGG TGGAGTTCCAGGAGGGCGTGGAGCTGGTGGATGTGCGAGTGCCCCTCTTCATCCGGCCTGAGGATGATGA TGAGAAGCAGCTGCTGGTGGAGGCCATTGATGTCCCTGTGGGCACTGCCACCCTTGGTCGCCGTCTGGTA AACATCACCATTATCAAGGAACAAGCTAGTGGGGTAGTGTCCTTCGAGCAGCCTGAATACTCGGTGAGTC GTGGAGACCAGGTGGCCCGCATCCCTGTCATCCGGCACATCCTGGACAATGGCAAGTCCCAGGTCTCCTA TAGCACACAGGATAATACAGCACACGGACACCGGGATTATGTCCCTGTGGAGGGAGAGCTGCTGTTCCAT CCTGGGGAGACCTGGAAGGAGTTGCAGGTGAAGCTACTGGAGCTGCAGGAGGTCGACTCCCTCCTGCGTG GCCGCCAGGTCCGCCGCTTCCAAGTCCAACTCAGCAACCCCAAGTTCGGAGCCCGCCTGGGCCAGCCCAG CACAACCACCGTTATTCTCGGTGAGCACGATGAAACGGACAGGAGTCTCATAAATCAAACACTTTCATCG CCTCCGCCACCCCATGGAGACCTGGGCGCCCCACAGAACCCCAATGCCAAGGCTGCCGGGTCCAGGAAGA TCCATTTCAACTGGCTGCCCCCTCCTGGCAAGCCAATGGGGTACAGGGTGAAGTACTGGATCCAGGGCGA CTCTGAGTCTGAAGCCCACCTTCTAGACAGCAAGGTGCCCTCAGTGGAACTCACCAACCTGTATCCCTAT TGCGACTACGAAATGAAGGTGTGTGCCTATGGGGCACAGGGTGAGGGGCCCTATAGCTCACTGGTGTCCT GCCGCACCCACCAGGAAGTACCCAGCGAGCCAGGGAGGCTGGCTTTCAATGTAGTCTCTTCTACGGTGAC GCAGCTGAGCTGGGCAGAGCCAGCTGAGACCAATGGCGAGATCACAGCCTACGAGGTCTGCTATGGACTG GTCAATGAGGACAACCGACCCATTGGACCTATGAAGAAGGTGCTCGTGGACAACCCCAAGAACCGGATGC TGCTCATTGAGAATCTGCGAGAATCCCAGCCATACCGATACACGGTTAAGGCGCGCAATGGGGCAGGATG GGGACCCGAGAGAGAGGCTATCATCAACCTGGCTACACAGCCCAAGCGGCCCATGTCCATCCCTATCATC CCAGACATCCCCATAGTGGACGCCCAGGGTGGAGAAGACTACGAAAACTTCCTTATGTACAGTGATGACG TCCTGCGGTCCCCAGCCAGCAGCCAGAGGCCCAGCGTTTCTGATGACACTGAGCACCTGGTGAATGGCCG GATGGACTTTGCCTATCCAGGCAGCGCCAACTCCCTGCACAGAATGACTGCAGCCAATGTGGCCTATGGC ACGCATCTGAGCCCACACCTGTCCCACCGAGTGCTGAGCACGTCCTCCACCCTTACCCGGGACTACCACT CTCTGACACGCACAGAGCACTCCCACTCAGGCACACTTCCCAGGGACTACTCCACCCTCACTTCCCTTTC CTCCCAAGATTCCCGTGGGGCTGTGGGTGTGCCTGACACACCCACTCGGCTGGTGTTCTCTGCCCTGGGG CCCACGTCTTTGAAGGTGAGCTGGCAGGAGCCACAGTGTGATCGGATGCTGCTGGGCTACAGTGTGGAAT ACCAGCTACTAAACGGCGGCGAGATGCATCGGCTCAACATCCCTAACCCTGGCCAAACCTCGGTGGTGGT AGAGGATCTCCTGCCTAACCACTCCTATGTGTTCCGGGTACGGGCCCAGAGCCAGGAGGGCTGGGGCCGA GAGCGAGAGGGTGTCATCACCATCGAGTCCCAGGTGCACCCGCAGAGCCCTCTCTGCCCCCTGCCAGGCT CAGCCTTCACTCTGAGCACCCCCAGCGCCCCGGGACCGCTGGTGTTCACTGCCCTAAGCCCAGACTCCCT GCAGCTCAGCTGGGAGCGGCCGAGGAGGCCCAATGGAGATATCCTTGGCTACCTGGTGACCTGTGAGATG GCCCAAGGAGGAGCACCAGCCAGGACCTTCCGGGTGGACGGAGACAACCCTGAGAGCCGGTTGACTGTAC CTGGCCTCAGTGAGAACGTTCCTTACAAGTTCAAGGTTCAGGCCAGGACGACCGAGGGCTTCGGGCCAGA GCGTGAGGGTATCATCACCATCGAGTCTCAGGTTGGAGGCCCCTTCCCACAGCTGGGCAGCCATTCTGGG CTCTTCCAGAACCCAGTGCAAAGCGAGTTCAGCAGCGTGACCAGCACGCACAGCACCACGACTGAGCCCT TCCTCATGGATGGTCTAACCCTGGGGACCCAGCGCCTGGAAGCAGGAGGCTCCCTCACCCGGCATGTGAC CCAGGAATTCGTGACCCGGACCTTAACGGCCAGTGGCTCTCTCAGCACTCATATGGACCAACAGTTCTTC CAAACCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_133663
Insert Size	5259 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_133663.2, NP_598424.2
RefSeq Size	6010 bp
RefSeq ORF	5259 bp
Locus ID	192897
Gene Summary	Integrins are heterodimers comprised of alpha and beta subunits, that are noncovalently associated transmembrane glycoprotein receptors. Different combinations of alpha and beta polypeptides form complexes that vary in their ligand-binding specificities. Integrins mediate cell-matrix or cell-cell adhesion, and transduced signals that regulate gene expression and cell growth. This gene encodes the integrin beta 4 subunit, a receptor for the laminins. This subunit tends to associate with alpha 6 subunit and is likely to play a pivotal role in the biology of invasive carcinoma. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) uses a different splice site and lacks an alternate in-frame exon in the 3' coding region, as compared to variant 1. The resulting protein (isoform 2) thus has two differences with a shorter C-terminus when compared to isoform 1.

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