Ubr1 (NM_009461) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI504731
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224911 representing NM_009461 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGACGAAGAGATGGACGGCGCCGAGAGGATGGACGTCAGCCCGGAGCCTCCCCTGGCCCCGCAGC GGCCGGCATCGTGGTGGGATCAGCAAGTTGATTTCTATACTGCTTTCTTACATCATTTGGCACAATTAGT GCCAGAAATTTATTTTGCTGAGATGGACCCAGATTTGGAAAAGCAAGAAGAGAGTGTACAGATGTCAATA CTCACTCCTTTGGAGTGGTACTTATTTGGAGAGGATCCGGATATTTGCTTAGAGAAATTAAAACACAGTG GAGCGTTCCAGTTGTGTGGGAAGGTTTTCAAAAGTGGAGAAACAACATATTCCTGTAGGGATTGTGCAAT TGATCCAACGTGTGTGCTCTGTATGGACTGCTTCCAAAGTAGTGTTCATAAAAACCATCGTTACAAGATG CATACTTCTACTGGAGGAGGCTTCTGTGACTGTGGAGACACAGAAGCGTGGAAAACTGGCCCTTTTTGTG TGGATCACGAGCCTGGAAGAGCAGGTACTACAAAAGAGAGCTTACATTGCCCATTGAATGAAGAGGTGAT TGCTCAAGCCAGGAGAATATTCCCTTCGGTGATAAAATACATTGTAGAAATGACTATATGGGAAGAAGAA AAGGAATTGCCTCCTGAACTGCAGATAAGGGAGAAAAATGAACGATACTATTGTGTCCTTTTCAACGATG AGCACCATTCGTATGATCATGTGATCTACAGTCTGCAGAGAGCTCTAGATTGCGAGCTTGCAGAGGCACA GCTGCACACGACTGCCATCGACAAAGAGGGTCGCCGGGCTGTCAAAGCAGGTGTGTATGCCACTTGCCAG GAAGCAAAGGAGGATATAAAGAGTCACTCAGAGAACGTCTCTCAGCACCCCCTCCATGTGGAAGTGCTGC ACTCCGTGGTTATGGCTCACCAGAAATTCGCTCTGCGCCTTGGCTCCTGGATGAACAAAATTATGAGCTA TTCAAGTGACTTTAGACAGATATTTTGCCAGGCCTGCCTCGTAGAAGAACCTGGCTCTGAAAATCCCTGT CTTATAAGCAGACTAATGCTTTGGGATGCAAAACTTTATAAAGGTGCCCGTAAGATCCTTCATGAATTGA TCTTTAGTAGTTTTTTTATGGAGATGGAATACAAAAAACTCTTTGCTATGGAATTTGTGAAGTATTATAA ACAGCTGCAGAAAGAGTACATCAGCGACGACCACGAGAGAAGCATCTCCATAACCGCCCTGTCCGTGCAG ATGCTCACCGTCCCGACCTTGGCCCGGCATCTTATTGAAGAGCAGAATGTTATTTCTGTCATTACTGAAA CGCTGCTAGAAGTTTTACCTGAATACTTGGACAGGAACAATAAATTCAACTTCCAGGGTTATAGCCAGGA CAAACTGGGAAGAGTCTACGCAGTTATATGTGACCTAAAGTATATCCTGATTAGCAAGCCTGTCATATGG ACAGAACGATTAAGAGCGCAGTTCCTGGAAGGTTTCCGGTCTTTTCTGAAGATTCTTACCTGTATGCAGG GAATGGAAGAAATCAGAAGACAAGTTGGACAACACATTGAAGTGGACCCTGACTGGGAGGCTGCCATCGC TATACAGATGCAACTAAAGAATATTTTGCTCATGTTCCAAGAGTGGTGTGCTTGTGATGAAGATCTCTTA CTGGTGGCTTATAAAGAATGTCACAAAGCTGTAATGAGGTGCAGTACAAATTTCATGTCCAGTACCAAGA CAGTAGTGCAATTGTGCGGTCATAGTCTGGAAACCAAATCCTACAAAGTGTCTGAGGACCTTGTAAGCAT ACACCTGCCACTCTCTAGAACACTTGCTGGTCTTCATGTACGTTTAAGCAGACTAGGTGCTATTTCAAGA CTGCATGAATTTGTGCCTTTTGACGGCTTTCAAGTAGAGGTCCTGGTGGAGTACCCGCTGCGCTGCCTGG TCCTGGTGGCTCAGGTTGTTGCTGAGATGTGGCGAAGAAACGGGCTCTCACTCATCAGCCAGGTTTTCTA TTATCAAGATGTTAAATGCAGGGAGGAAATGTACGATAAAGATATCATCATGCTTCAGATTGGAGCATCT ATAATGGATCCCAACAAGTTCTTGTTACTGGTACTTCAGAGATATGAACTTACTGATGCTTTTAACAAGA CCATATCCACAAAAGACCAGGATTTGATTAAACAGTATAATACATTAATAGAAGAAATGCTTCAGGTCCT CATCTATATTGTGGGAGAACGTTATGTACCTGGAGTGGGAAATGTTACCAGAGAGGAGGTTATAATGAGA GAGATTACTCACTTACTTTGCATTGAGCCCATGCCACATAGTGCCATCGCCAGAAACCTACCTGAGAACG AAAATAATGAAACTGGCTTAGAGAATGTCATAAACAAAGTGGCCACATTTAAGAAACCAGGTGTGTCGGG CCATGGAGTTTATGAATTGAAAGATGAATCACTGAAAGACTTCAATATGTACTTTTACCATTATTCTAAA ACACAGCATAGCAAGGCTGAACATATGCAGAAGAAAAGGAGAAAACAAGAAAATAAAGATGAAGCATTGC CGCCGCCACCTCCTCCGGAGTTCTGCCCTGCTTTCAGCAAAGTAGTCAACCTGCTCAGCTGTGATGTTAT GATGTACATCCTCAGGACCATCTTTGAGCGGGCAGTGGACATGGAGTCTAATCTGTGGACAGAAGGGATG CTGCAGATGGCGTTCCATATATTGGCACTGGGCTTGCTGGAAGAGAAGCAGCAGCTTCAGAAAGCTCCTG AAGAGGAAGTGGCTTTTGACTTTTACCATAAGGCTTCAAGATTGGGAAGTTCAGCCATGAATGCTCAGAA TATACAAATGCTCTTGGAAAAACTCAAAGGAATCCCCCAATTAGAAAGCCAGAAGGACATGATAACATGG ATACTCCAGATGTTTGACACAGTGAAGCGATTAAGAGAAAAATCTTGTTTAGTTGTGGCAACCACTTCAG GACTGGAGTGCGTTAAGAGTGAGGAGATTACTCATGATAAAGAAAAGGCAGAAAGGAAGAGAAAAGCTGA GGCCGCTAGGCTTCATCGCCAGAAGATCATGGCCCAGATGTCTGCCTTACAGAAAAACTTCATTGAAACC CACAAACTCATGTATGATAATACGTCAGAAGTAACAGGGAAGGAAGACTCCATTATGGAGGAAGAGAGCA CCTCAGCAGTCAGTGAGGCCTCTAGAATTGCTCTGGGCCCTAAACGGGGCCCGGCTGTTACTGAAAAGGA GGTGCTGACGTGCATCCTCTGCCAAGAAGAACAAGAGGTGAAACTAGAAAATAACGCCATGGTATTGTCA GCATGTGTGCAGAAATCCACCGCCCTAACCCAGCACAGAGGGAAGCCTGTGGACCACTTAGGGGAAACAC TGGACCCTCTTTTCATGGATCCAGACTTGGCACATGGAACTTATACAGGAAGCTGTGGTCATGTAATGCA TGCAGTGTGCTGGCAGAAGTATTTTGAAGCTGTGCAGCTGAGCTCGCAGCAGCGCATTCACGTAGACCTG TTTGACCTGGAGAGCGGCGAGTACCTATGCCCGCTCTGCAAGTCTCTCTGCAATACTGTCATCCCCATCA TCCCTTTGCAGCCGCAGAAGATCAACAGTGAGAATGCGGAGGCTCTTGCTCAACTTTTGACCTTGGCCCG GTGGATACAGACTGTCCTTGCCAGAATATCGGGTTATAATATAAAGCATGCTAAAGGAGAAGCCCCAGCA GTTCCTGTCTTGTTTAATCAAGGAATGGGGGATTCGACTTTTGAGTTTCATTCCATCCTGAGTTTTGGAG TTCAGTCTTCGGTGAAATATTCAAATAGTATCAAGGAAATGGTCATTCTCTTCGCCACAACAATTTACAG AATTGGCCTGAAAGTGCCTCCTGATGAACTAGACCCACGAGTGCCCATGATGACCTGGAGCACGTGTGCG TTCACCATCCAGGCAATCGAAAACCTGTTGGGAGATGAAGGAAAACCTCTATTTGGAGCACTTCAAAATA GACAGCATAACGGTCTGAAGGCGCTAATGCAGTTTGCAGTTGCACAGAGGACTACCTGCCCTCAGGTCCT GATACACAAACATCTGGCTCGGCTCCTGTCAGTTATTCTTCCTAACCTGCAATCAGAAAATACACCAGGC CTTCTGTCCGTGGATCTCTTCCATGTTCTGGTCGGCGCAGTCTTAGCGTTCCCATCCTTGTATTGGGATG ACACCGTGGATCTGCAGCCGTCACCACTTAGTTCTTCATATAACCACCTCTATCTCTTCCATCTGATCAC CATGGCGCACATGCTTCAGATACTCCTTACAACAGATACAGATCTGTCTTCAGGGCCGCCGCTTGCTGAG GGTGAAGAGGATAGTGAGGAGGCTCGCTGTGCATCTGCTTTCTTTGTGGAAGTGTCGCAGCACACAGACG GCCTCGCTGGGTGCGGTGCTCCCGGCTGGTACCTGTGGCTCTCCCTGAGGAACGGCATCACCCCTTACCT CCGCTGTGCTGCACTGCTTTTCCACTATTTACTTGGAGTAGCTCCGCCTGAAGAACTGTTTGCCAATTCT GCTGAAGGAGAATTCAGTGCACTCTGTAGCTATCTATCTTTACCCACAAATTTGTTCCTGCTTTTCCAGG AATATTGGGATACCATAAGGCCCTTACTACAGAGGTGGTGTGGAGATCCTGCCTTACTCAAGTCTTTGAA GCAGAAAAGTGCTGTGGTCAGGTACCCTAGAAAAAGAAATAGTTTGATAGAGCTTCCTGAGGACTACAGC TGTCTTCTAAATCAGGCTTCTCACTTTAGGTGTCCACGGTCTGCAGATGATGAGCGAAAGCATCCTGTCC TCTGTCTTTTCTGTGGGGCCATCCTGTGTTCTCAGAACATCTGTTGCCAAGAAATAGTGAATGGGGAAGA GGTTGGAGCGTGCGTTTTTCATGCGCTTCATTGTGGTGCTGGAGTCTGCATTTTCTTAAAAATCCGAGAA TGCAGGGTGGTCCTGGTGGAAGGAAAAGCCAGAGGCTGTGCCTACCCAGCCCCTTACTTGGATGAATATG GAGAAACAGACCCAGGGCTAAAGAGAGGAAACCCACTTCATTTATCTCGGGAGCGGTATCGGAAGCTGCA TTTGGTCTGGCAACAGCACTGCATTATAGAAGAGATTGCTCGGAGCCAGGAGACTAATCAGATGCTATTT GGATTCAACTGGCAGTTACTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_009461
Insert Size	5274 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_009461.2, NP_033487.2
RefSeq Size	7765 bp
RefSeq ORF	5274 bp
Locus ID	22222
UniProt ID	O70481
Gene Summary	E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. May be involved in pancreatic homeostasis. Binds leucine and is a negative regulator of the leucine-mTOR signaling pathway, thereby controlling cell growth (By similarity).[UniProtKB/Swiss-Prot Function]

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