Tubgcp6 (NM_001163319) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI843275; D430023H11; GCP-6; mKIAA1669
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224921 representing NM_001163319 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAGCATCACCCAGCTGTTCGATGACCTCTGCGAGGCTCTCTTACCTGCTGCCCAAGCTCGCCCCG GCCAGCGCAGTGTGAACAGGAAGAGGGCGAAGCGGAGCCTTAAGAGGGTGGCCTACAATGCCCTTTTCGC AAATCTTTTTCAAGAGGACACCCATCAACGGCAGCCCGACTCGTCGAAGCTCCCCGTGAAAAACAAGGTC CTCATGCTGTCCTTCGATTTGAGGGTGGGGGGCTTGGGGCCAGAGGCTGACCGTTTGGAGGAGCTCGTGG AGAAACTTGAAGCAGCCCCTGACTGTCCTTTTGTGGAGGTAGCATCAGTGTTGGACCTCTTAGTTCAGCT GGCAGGAAGTGGCCCCCCTCAAGTGCTGCGTAGAAAACGGGATTACTTCTTTAACAACAAGCATGCAGGC CGAAACATACCCTACAGCGGCTACGATTGCTATGACTTGAGTGTGTTTGAAATGGACGTTCGGTCATTTA TCTCTGGGGAGGAGAATTTGTGTCATCACACGGTCCAGGAAGCGCTTCAGGTGATGGAGGCTGCCCCAGG CACTGGCCTGCCCACCGTTGGGCTCTTCTCAATTGGTGACTCTTGTGGTGACAGGTTTGAGAGAGACACC AGGGTCTCCCTCTTTGGTGCACTTGTGCATAGCCGCACCTATGACATGGATGTGCGACTGGACCTACCAC CTGTGCCAGACAGCGCGGACTTCTCTGGACTAGCCATCAAGGTCCCACAGATTGTGGATCAGTGGGAAGA TGAAGGTTTCCAGTCAGCTTCCAATTTAACTCCCGACTCCCAGTCTGAACCAAGCATGACCCCCGATTTG GACCTGTGGGAAGCTGTGCTCACATACGAAGCCAGCAAGCGGAGGTGCTGGGAGCGTATTGGATGCCCCC CTGGTCACCGAGAGGAGCCCTACCTCACTGAGGCAGGACGGGATGCCTTTGACAGGTTCTGCAGGCTCCG TCATGGAGAGCTACAGGCCCTCAGTGGGGGCCTTCTACAGGCCCCGAAGCCTGTGCTGGTGGAGGAAAGC GAGCTGGTGAAAGACTCACTGAACGTTCTGCTGGGCGTTGTTTCTGCTACATTTTCCCTGTGTCGGCCGA CTCAAGCCTTTGTAGTGGAGCCGGGTGTGCATGTGTCAGGTGCATCTCCTGAAAGCATCAGCAGCATCCT CTCAGAGGTGGCGGAGTATGGAACCTGTTACACACGGCTGAGCCATTTCTCTCTGCAACCTGTTGTAGGC TCCTTGTGCAGCAGGGGCCTTGTTTTCCAGGCCTTCACCAGCGGCCTAAGGAGGTACTTGCAGTATTACC GTGCCTGTGTGCTCTCCACTCCACCCACCCTCAGTCTCCTCACCATTGGCTTTCTCTTCAAGAAGCTGGG CCGGCAGCTCAGGTACTTGGCTGAGCTTTGTGGTGTTGGCACTGTATCCCTGGCGACCAGTGGAGAACCC AGGGCTGTGTTCCCCACTGGGGTGAAGCTTCTGTCCTACCTCTACCAGGAAGCACTGGATAACTGCAGTA ATGAGCACTATCCCGTTTTGCTGTCCCTCCTGAAGACCAGCTGTGAGCCCTACACACGGTTCATCCACGA TTGGGTGTACAGTGGAGTCTTCAGAGATGTTTATGGGGAGTTCATGATCCAGGTTAACCATGAGTACCTC AGCTTCAGAGACAAGTTTTACTGGACCCATGGCTACGTGCTCATTTCCAAAGAGGTGGAGGACTGTGTTC CTGTATTCTTGAAACACATTGCTCATGATGTGTATGTCTGTGGGAAGACCATCAACCTACTGAAGCTCTG CTGTCCCCGGCATTACCTTTGTTGGTCTGATGTCCCTGTCCCTCGGATCTCGGTGATTTTCTCTCTTGAG GAACTGAAGGAGATTGAGAAGGACTGTGCTGTCTATGTTGGGCGGATGGAGAGAGTCGCACGTCATAGCT GTATCAGCAAGGAGGAAAAGGAATTACGTATGGAAATTGCGAAACAAGAACTAATTGTCCATGCCCGGGA AGCCGCGTCCAGGGTCCTGAGCGAGTTGAGTGACCGGCAGATGGCAGAACAGATTGCTCAGGACACTCGG AAGCGAGAGCAGTTTCAGCGACTGAAGGAACAGTTTGTGAAGGATCAGGAGCGACGCCTGGCAGCCAGAC AAGAAGAGCTAGATGATGACTTCAGCTATGCCCGTGAGCTCCGTGACCGGGAGAAGAGGCTAAAGGCCTT GGAGGAAGAGCTGGAGAGGAAGGCCAGGCAGGCGCTGGTTGACCATTATAGCAAGTTGTCTGCAGAGGCA GCTCGTCGAGAGCAGAAGGCTCTGTGGAGAATCCAGAGGCATAGGCTGGAAAGCGCACGACTTCGTTTTC TCCTAGAAGACCAGAAGTGCATTCAGGAGATGCTGAGAGACATGGAGGCTCAGCAGCCCCAGGAGCCGCC GAGTGTGTTCCCATCCACAGGCTCTCAGGTCACATCTACAGGTCCTGAGCACGCAGGGGAAGGCCATAGC TGTGATCCGGGGTTCACAGAGCTGCATTGGGGTTGTCCAAGCCTACCGTGTGCATCGACACCATCAGTCC CCAAGTCTGCCACAGAGGGAGCTGATGACTCGGGAGCTGGGCCATTCTCGACTGGTCTTAGCATCACAGA CTTTCTGCCCGTGGACTCTGGAGAGGAACAGCCTGTGGAGAACACTGGCGTTCCCTTTTTAGAGGTGGCT CTGCAGACCATCTGCTCAGACCTATCCCCTGTAGCCCCTGAGCCAGCGGCACTCACAGCAGGGGGCCCCC AGGCTACCCAGTCAGAGTATGATTTCAATACCATACTGAGGCCAGCTATGGCTACCTCCCTCTCACCAGG ACCCTTCCAGGATGTTCAGAATAGCGTGGACAGTGACAAACAGCATCTACTGGGGGATATGTCCACAAAA GTAGATTCTTATATCCATGATATGCAAGAGACTTTGCCTTGCCCACATCCACTCAGTCATGCCACTCCTG TGGAGGGGAGCCTCCAGCCTGTAGGCCAGCTCCTTGAACACATGTCAGAGACTACTGTCTCTACAGAGAG CCATGCTTCTGGGATGGCTCCTTGTCAGCAACTTAGCATTTCCAGACACGTGTCAGATGCTAACATCAAG GTGGGGGATTATATGTCAGATGTGGCTCTTCCTCGGCCACGGTGGAATGTCCATGGCCATGTGTCAGAGG CCAGCATTGGAGTTGGGGAGAATATGGCCGAAGTGGCTCCTTCTAGGCCACGGTGGAATGTCCACGGACA TGTGTCAGATGCCAGCATCAAGATAGGGGAGAATATGTCAGATGTGGCTCCTTCTCGGACACGGTGGAAC ATTCATGGGCATGTGTCAGATGCCAGCATCAAGGTAGGGGAGAATGTATCAGATGTGACTCCTTCTCGGC CGCGGTGGAATGTCCACGGGCATGTGTCAGAGGCCAGCATCAAGGTAGGGGAGAATGTATCAGATGTGAC TCCTTCTCGGCCGCGGTGGAATGTCCACGGGCATGTGTCAGAGGCCAGCATCAAGGTAGGGGAGAATGTA TCAGACGTGACTCCTTCTCGGCCGCGGTGGAATGTCCACGGGCATGTGTCAGATGCCAGTATTAGGATTG GGGAAAATGTATCAGATACGGATCTTGATCTTCAGCAACGTGGATGTGCCCAGCCTCCCTTGATTCTGGA GGAGCCCTTGCCAGAAGCTGAGGCTGACCTCAAGCCCCATCAGTGTCCCCCTGCTCACGTGTCTGAGGCA GTGCTTGGTGTGGAAGCACAGAGCCCTGCTCTAGAATGTGGGCCACAGCTACCTGAAAAAACGAAGCCTA CCGTGTGTTCTGGCTTTGGCAGAACAGAGGAAGGTAGCCTACAGACCAAGACCCTTGTGGCTGAGCCTAG CATGCTGGGAAGTGGCATCCCTGAAGAGAAAGGCCCAGGGAAGAGTAGGGATGCTGAAGACCTTTCTCCA TGTTTGCCTTCAAGCTCACAGGAAGACACAGCTGTCCCCAGCAGCCCAGGCCCTAGTGATGAGGTATCAA ACACAGAAGCTGAGGCCAGACGCTGGGGCAAGGAGCAAGCCTATTTGACAGACCTCACAAAATTGTACCA CCTGGAGCAGTACCCGGACAGCTATGATTCCATGTCAGAGCCTCCTGTTGCTCACCTGGTCCACCACATG CTTCCCCGGGCCTTTGCTTTCCCTGTTGACCCCCAAGTTCAGTCAGCAGTAGACGAGAGTGCAGTGCAGC TGAGCGAGCTGCTGACATTGCCTGTGCTTATGAAGCGTTCCCTTATGGCGCCTCTGGCTGCCCATGTCAG CCTGGTCAGCAAAGCCGCAGTTGACTACTTCTTTGTGGAGCTGCACCTGGAGACACACTTTGAGGCACTG CGCCATTTCCTGCTGATGGAAGATGGAGAGTTCGCTCAGTCCCTTAGTGACCTGCTCTTTGAGAAGCTAG GGGCTGGGCAGACGCCTGGAGAGCTGCTCAACCCACTGGTCCTTAACAGCATCCTGAGCAAGGCCCTGCA GTACAGCCTCCACGGGGACACCCCGCATGCTTCCAACCTGTCTTTTGCTCTCAAGTACCTGCCTGAGGTG TTTGCCCCGAATGCCCCAGATGTGCTGAGCTGCCTGGAGCTCAGGTACAAGGTCGACTGGCCCCTCAACA TTGTTATCACCGAGAGCTGCCTGAACAAGTACAGTGGCATCTTCTCCTTCCTGCTGCAGCTGAAGCTCAT GATGTGGACACTCAAAGACATCTGCTTCCACCTCAAGCGCACAGCCCTCGTGAGCCACACGGCTGGCTCA GTGCAGTTCCGACAGCTGCAGCTGTTCAAGCATGAGATGCAGCACTTTGTGAAGGTCATCCAGGGCTACA TTGCTAACCAGATCCTGCATGTCAGCTGGTGTGAGTTCCGAGCCCGTCTAGCTGTGGTGGGTGACCTGGA GGAGATTCAGCGGGCCCATGCCGAGTACCTGCACAGGGCTGTTTTCAGGGGCCTACTGACAGAGAAGGCA GCGCCAGTCATGAACATCATCCATAGCATCTTCAGCCTGGTGCTCAAGTTCCGAAGCCAGCTCATCTCTC AGAACTGGGGCCCGGCCACCGGCCCCCGTGGTGCCGAGCACCCCAACTTCCCCCTCATGCAGCAGTCCTA CAGCACCTTCAAGTACTACTCCCACTTCCTCTTCAAAGTAGTGACCAAGCTGGTGAACCGAGGCTATCAG CCCCACCTGGAAGACTTTCTGCTTCGCATCAACTTCAACAACTACTACCAGGACTCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001163319
Insert Size	5310 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001163319.1, NP_001156791.1
RefSeq Size	6957 bp
RefSeq ORF	5310 bp
Locus ID	328580
UniProt ID	G5E8P0
Gene Summary	Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome.[UniProtKB/Swiss-Prot Function]

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