Kidins220 (NM_001081378) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	3110039L19Rik; AI194387; AI316525; C330002I19Rik; mKIAA1250
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224933 representing NM_001081378 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGTTCTTATATCACAGAGTGTGATAAATTATGTGGAAGAAGAGAATATTCCTGCTCTGAAAGCCC TTCTTGAGAAGTGCAAAGATGTAGATGAGAGGAACGAGTGTGGCCAGACGCCACTGATGATAGCTGCTGA ACAGGGCAATGTGGAAATAGTGAAAGAACTCATTAAGAATGGAGCCAACTGCAACCTGGAAGACCTGGAT AACTGGACTGCGCTTATATCAGCATCTAAAGAGGGGCACATCCACATTGTGGAAGAGCTGCTGAAGTGTG GGGCCAATCTGGAGCACCGTGACATGGGGGGATGGACAGCTCTCATGTGGGCGTGCTACAAAGGCAGGAC GGATGTTGTGGAGTTGCTTCTTTCTCATGGTGCCAACCCAAGTGTCACTGGTTTGTACAGTGTCTACCCA ATCATCTGGGCAGCGGGCAGAGGCCACGCCGACATAGTGCATCTTCTGCTGCAGAATGGTGCGAAAGTCA ATTGCTCTGATAAGTATGGAACAACTCCTTTGGTGTGGGCTGCCCGAAAGGGTCATTTGGAATGTGTGAA GCATTTGTTGGCCATGGGAGCTGATGTTGATCAAGAAGGAGCTAATTCGATGACTGCACTGATTGTGGCA GTGAAAGGGGGCTACACACAGTCAGTGAAGGAGATTCTGAAAAGGAATCCAAACGTGAACCTGACTGATA AGGACGGAAACACAGCCCTGATGATTGCGTCGAAGGAGGGACATATAGAAATTGTGCAGGACCTGTTGGA CGCCGGAACATACGTGAACATACCTGACAGGAGTGGGGATACTGTATTGATTGGCGCTGTGAGAGGTGGT CATGTTGAAATTGTTCGAGCACTTCTCCAAAAATACGCTGATATAGACATTAGAGGGCAGGACAATAAGA CTGCACTGTATTGGGCTGTTGAGAAAGGCAATGCGACAATGGTGAGAGATATCCTGCAGTGCAATCCTGA TACGGAAATATGCACGAAGGATGGCGAGACACCACTAATAAAGGCCACCAAGATGAGAAATATTGAAGTG GTGGAGCTGCTGCTGGATAAGGGGGCTAGGGTGTCTGCTGTGGACAAGAAAGGTGACACTCCCCTGCACG TTGCCATCCGAGGGAGGAGCCGGAGACTGGCAGAACTTCTTTTACGGAACCCCAAAGATGGACGGTTACT TTACAGGCCCAACAAAGCAGGCGAGACTCCCTACAACATTGACTGCAGCCACCAGAAAAGCATTTTAACT CAAATATTTGGAGCCAGACACTTGTCTCCTACGGAAACAGACGGTGACATGCTTGGTTACGATTTGTATA GCAGCGCCCTGGCAGATATTCTCAGTGAGCCCACCATGCAGCCACCCATCTGTGTGGGCTTGTATGCACA GTGGGGAAGTGGGAAGTCCTTCTTACTCAAGAAACTAGAAGATGAAATGAAGACGTTTGCAGGGCAGCAG ATTGAGCCCCTTTTCCAGTTCTCGTGGCTCATCGTGTTCCTCATCCTGCTGCTGTGTGGGGGGCTCGGCC TGGTGTTTGCTTTCACAGTTGATACAAACCTCGCCATAGCGGTTTCATTGAGTTTCCTAGCTCTCTTATA CATATTCTTCATCGTCATTTACTTCGGTGGACGGCAGGAAGGAGAGAGTTGGAACTGGGCCTGGGCTCTC AGCACCAGACTGGCAAGGCACATTGGGTACCTGGAGCTCCTCTTCAAGCTGATGTTTGTGAACCCACCCG AACTACCAGAGCAAACGACAAAGGCTTTGCCTGTGAGGTTTCTGTTTACAGATTACAATAGACTCTCCAG TGTTGGGGGAGAGACTTCCTTGGCTGAAATGATCGCGACCCTCTCAGATGCTTGTGAGAGAGAATTCGGC TTTCTGGCTACCCGGCTGTTTCGAGTATTTAAGACTGAAGATTCTCAGGGTAAAAAGAAATGGAAAAAAA CATGCTGTCTCCCGTCTTTTATAATCTTTCTTTTTATTGTTGGCTGCATTATTGCTGGAATTACTCTTTT GGCTATATTCCGAGTTGACCCAAAACATCTGACAGTGAATGCTATCCTCATATCCATTGCATCTATCGTG GGGTTGGCCTTTGTGCTGAACTGTCGAACATGGTGGCAAGTGCTGGACTCTCTCCTGAATTCTCAGAGAA AACGCCTCCATAGTGCTGCCTCCAAATTACATAAGCTGAAAAGTGAAGGATTCATGAAAGTCCTGAAGTG TGAGGTGGAGCTGATGGCCAGGATGGCCAAGACCATCGACAGCTTCACGCAGAATCAGACGAGGCTGGTG GTTATCATTGACGGCCTGGACGCCTGTGAGCAAGATAAAGTGCTGCAGATGCTGGACACTGTCCGAGTTC TGTTTTCAAAGGGCCCTTTTATTGCCATTTTTGCAAGTGATCCACATATTATTATAAAGGCCATCAACCA GAACCTGAATAGTGTGCTTCGTGATTCAAATATAAATGGACATGACTATATGCGCAATATAGTTCATTTA CCAGTTTTCCTTAATAGTCGTGGGCTTAGCAATGCAAGAAAATTTCTTGTAACTTCAGCAACAAATGGGG ACATTTCGTGCTCAGAAGCAACAGGAGTGCAGGAGGATGCTGACAGAAGAGTTTCACAGAACAGCCTTGG GGAGATGACAAAGCTTGGTAGCAAAACGGCCCTCAACAGAAGGGATACTTACCGCAGAAGGCAGATGCAG AGAACCATCACGAGGCAGATGTCCTTTGATCTCACAAAGCTGCTGGTTACTGAGGATTGGTTCAGTGACA TCAGTCCACAAACCATGAGACGGTTACTCAATATTGTTTCGGTGACAGGGCGGCTACTAAGAGCCAATCA GATTACTTTCAACTGGGACAGGCTAGCCAGCTGGATCAACCTTACTGAGCAGTGGCCATACCGGACGTCC TGGCTCATACTATATCTGGGAGAGACTGAAGGTATTCCTGACCAGATGACGCTAAAGACCATCTATGAGA GAATATCAAAGAATATCCCAACAACTAAAGATGTTGAGCCACTTCTTGAAATTGATGGAGATATAAGAAA TTTTGAAGTGTTTTTGTCTTCAAGGACTCCAGTTCTTGTGGCTCGAGATGTAAAGACCTTTTTGCCATGC ACGGTAAACCTGGATCCCAAACTACGGGAGATCATTGCAGATGTTCGAGCTGCAAGAGAACAGATCAACA TAGGAGGCCTTGCGTATCCCCCGCTCCCTCTGCACGAAGCCCCTCCTCGGCCTCCTTCTGGGTACAGTCA GCCTGCATCTGTCTGCTCCTCCTCAGCATCTTTCAATGGGCCGTTCCCAGGCGGCGTTGTGTCCCCACAG CCCCACAGCAGCTACTACAGCGGCCTGTCGGGACCCCAGCACCCTTTCTACAACAGGCCATTCTTTGCCC CATACCTTTACACGCCAAGGTATTACCCTGGCGGTTCCCAACATCTCATCTCACGTTCATCAGTAAAAAC GAGTTTGCCCAGAGATCAGAACAATGGCCTAGAGGTAATCAAGGAGGATGCTGCTGAGGGCCTTCCTTCA CCCACAGCCTCCTCGAGGGAGAAGAGCTGGACAAGAAAGCAGCTGATGGAGCTGTGTGACTCTGGGTTTA ACAAACAGAGACAGGCATCCGTCCCTGCCACAGGCACCTCTCTGTTACTGAGTTCAATGACTGTGGATAT CGTGTGTGAGAAACTGAGACAGATAGAAGGGCTGGACCAGGGCATGCTGCCTCAGTATTGCACAACCATC AAAAAGGCAAACATAAATGGCCGGGTATTGGCTCAGTGTAATATTGATGAGCTGAAGAAAGAGATGGCTA TGAACTTTGGAGACTGGCATCTTTTTAGAAGCATGGTCTTAGAAATGAGAAGTGTGGAAAACCAGGTGGT CCCCGAAGACCCACGTTTTCTCAATGAAAACTCCAGTGCCCCCGTTGCTCATGGGGAATCTGCTCGCCGC ACTTCTCACAGTGAACTGCCCCACACGGAGCTTTCCAGCCAGACTCCCTACACCCTGAACTTCAGCTTTG AAGAACTGAACACGCTTGGCCTAGACGAGGGAGCCCCACGGCACAGTAATCTGAGTTGGCAGTCACAAAC TCGCAGAACCCCAAGTCTCTCGAGTCTCAATTCCCAGGACTCCAGTATTGAAATTTCAAAGCTTACTGAT AAGGTGCAGGCCGAGTATAGAGATGCCTATAGAGAGTACATTGCTCAGATGTCCCAGTTAGAAGGGGGCA CAGGGTCGTCAACAATAAGCGGCAGATCTTCTCCACACAGCACATACTACATAGGTCAGAGTTCTTCAGG GGGCTCCATTCATTCTAATCTAGAACAAGAAAGGGGGAAGGAGAATGAGCTAAAGCAGGAGGAGGGGCGG AAGTCATTTTTAATGAAGAGGGGGGATGTCCTAGATTACTCCTCATCAGGGGTGTCCACTAATGAGGCCT CACCCCTGGACCCCATTACTGAGGAAGATGAGAAATCCGACCAGTCAGGTAGTAAGCTGCTCCCAGGCAA GAAGTCTTCAGAACGGCCTAGTCTCTTCCAGACAGACTTGAAGCTTAAGGGAAGTGGTCTGCGCTACCAG AAACTGCCCAGTGATGAAGATGAGTCTGGGACAGAAGAGTCAGATAATACCCCACTGCTCAAAGATGATA AGGATAAAAAGGCCGAAGGCAAAGCAGAGAGAGTGGCTAAGTCTCCAGAGCACAGCGTCGAGCCTATCAG AACATTCATCAAAGCAAAAGAGTATTTGTCGGACGCCCTCCTCGACAAGAAAGATTCCTCCGATTCGGGA GTGAGGTCCAATGAGAGTTCTCCCAACCACTCTCTCCACAATGAGGCAGCGGATGACTCCCAGCTGGAAA AGGCGAATCTCATCGAGCTTGAAGATGAGGGCCACAGTGGGAAGCGAGGGATGCCACACAGTCTGAGTGG CCTGCAAGATCCTGTCATAGCTCGGATGTCCATTTGCTCGGAAGACAAGAAAAGCCCCTCTGAGTGCAGC CTGATCGCTAGCAGCCCTGAAGAAAGCTGGCCTTCGTGCCAGAAGGCCTACAATCTAAACCGAACACCCA GCACTGTGACTCTGAACAACAACACTGCGCCCACCAACAGAGCCAATCAGAACTTTGATGAGATAGAGGG AGTCAGGGAAACATCCCAGGTCATTTTGAGGCCTGGGCCCAGTCCCAACCCAACTGCTGTGCAGAATGAA AACCTGAAGAGCATGGCGCACAAGCGAAGCCAGCGCTCCAGTTACACCAGGCTCTCGAAAGATGCGTCTG AGCTGCATGCAGCCTCCTCAGATAGCACGGGCTTTGGGGAAGAGAGAGAAAGCATTCTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001081378
Insert Size	5382 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001081378.1, NP_001074847.1
RefSeq Size	7402 bp
RefSeq ORF	5382 bp
Locus ID	77480

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