Chd2 (NM_001081345) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2810013C04Rik; 2810040A01Rik; 5630401D06Rik; AI851092; BC029703; CHD-2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224958 representing NM_001081345 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGAGAAATAAGGACAAAAGCCAAGAGGAGGACAGTTCGCTACACAGCAATGCATCGAGTCGTTCAG CTTCTGAAGAAGTGTCGGGGTCAGACTCAGGCAGTCAGTCGGAAAGTGAGCAGGGCAGCGAGCCCGGAAG TGGACATGGCAGCGAGTCCAATAGCAGTTCGGAGTCTTCTGAGAGCCAATCAGAGTCTGAGAGTGAATCG GCAGGTTCCAAATCCCAGCCAGTCCTTCCGGAAGCCAAAGAGAAGCCTGCCTCTAAGAAGGAACGGATAG CTGATGTGAAGAAGATGTGGGAAGAATATCCTGATGTTTATGGGGTTAGGAGGTCAAATCGAAGCAGACA AGAACCATCACGATTTAATGTTAAGGAAGAGGCAAGTAGCGGGTCTGAGAGTGGGAGTCCGAAAAGAAGA GGCCAGAGGCAGCTGAAAAAACAAGAAAAATGGAAACAGGACCCCTCAGAAGACGAACAGGAACAGGGAA CCAGTGCAGAGAGTGAGGCAGAACAAAAGAAAGGAAAAGCTAGAAGACCGGTCCCTAGAAGAACAGTGCC CAAACCTCAAGTTAAAAAGCAACCTAAGATTCAGCGTGGAAAGAGGAAAAAACAAGAGTCTTCAGATGAC GACGACGATGATGATGAAGCTCCCAAAAGGCAGACTCGCCGAAGAGCTGCTAAAAACGTGAGTTACAAAG AAGATGATGACTTTGAGACTGATTCAGATGATCTCATTGAGATGACTGGAGAAGGAGGTGATGAACAGCA AGATAATAGTGAAACTATTGAAAAGGTCTTAGATTCAAGACTGGGAAAGAAAGGAGCCACTGGAGCGTCT ACCACTGTGTATGCTGTTGAAGCTAATGGAGATCCTAGCGATGACTTTGACACGGAGAGGGAGGAAGGTG AAGTCCAGTACCTTATCAAGTGGAAGGGCTGGTCTTATATTCACAGCACATGGGAGAGTGAAGACTCCTT ACAGCAACAGAAAGTGAAGGGCCTAAAGAAACTGGAGAATTTCAAGAAGAAAGAGGATGAGGTCAAGCAA TGGCTAGGGAAAGTTTCTCCTGAAGATGTTGAATATTTCAGTTGCCAACAGGAGCTGGCATCAGAGTTGA ATAAGCAGTATCAGATAGTAGAGAGAGTAATAGCTGTGAAGACAAGTAAATCTACACTGGGCCAAACAGA TTTCCCAGCTCACAGTCGGAAGCCAGCACCTTCAAATGAGCCTGAGTATCTGTGCAAGTGGATGGGACTA CCCTACTCAGAGTGCAGCTGGGAAGATGAAGCCTTGATTGGAAAGAAATTCCAGAATTGTATCGACAGCT TCCACAGTCGAAACAACTCCAAAACCATTCCCACAAGAGAATGCAAGGCTCTAAAGCAAAGGCCGAGATT TGTGGCTTTGAAGAAGCAGCCAGCTTATTTAGGAGGGGAGAGTCTGGAGCTCCGAGACTATCAGCTGGAG GGCCTCAACTGGCTGGCTCACTCCTGGTGCAAAAGTAACAGTGTAATCCTTGCTGATGAAATGGGCCTAG GAAAGACCATCCAGACCATATCGTTTCTCTCCTACCTGTTCCACCAGCACCAGCTCTATGGGCCTTTTCT TATAGTTGTTCCCTTGTCCACCCTTACCTCATGGCAGAGAGAGTTTGAGATCTGGGCACCAGAGATTAAT GTCGTGGTTTACATAGGTGACCTGATGAGCAGAAATACGATAAGGGAATATGAGTGGATTCATTCTCAAA CCAAAAGGTTGAAGTTCAATGCACTTATAACAACATATGAGATCCTCCTAAAAGATAAGACCGTGCTGGG CAGTATTAACTGGGCCTTCCTGGGAGTAGATGAAGCCCATCGGTTGAAGAATGATGACTCTTTATTGTAT AAAACTCTGATTGATTTCAAGTCCAACCATAGGCTACTGATCACGGGCACCCCTCTTCAGAATTCCCTCA AGGAACTTTGGTCCTTGCTGCACTTTATTATGCCGGAGAAGTTTGAGTTCTGGGAAGATTTTGAAGAAGA CCATGGAAAAGGGAGAGAGAATGGCTACCAGAGTCTTCATAAGGTACTAGAGCCTTTTCTTCTTCGAAGA GTCAAAAAAGATGTGGAGAAATCCCTTCCTGCCAAAGTGGAGCAGATTCTCAGGGTGGAGATGTCAGCCC TTCAGAAACAGTATTATAAATGGATCCTGACCAGGAATTACAAGGCTCTTGCCAAAGGGACCAGAGGCAG CACATCTGGCTTCCTTAATATCGTGATGGAGCTGAAAAAGTGCTGCAACCACTGTTATCTGATCAAAGCT CCCGAGGACAGCGAGAGGGAGAGCGGCCAGGAGGTCCTTCAGTCTCTAATTAGGAGCAGTGGGAAGCTGA TCCTGTTAGACAAACTGTTGACAAGACTCCGAGAAAGGGGAAACAGAGTCCTTATCTTCTCTCAGATGGT GAGGATGTTGGATATCCTGGCCGAGTACCTGACTATCAAACACTATCCTTTCCAGCGCCTGGATGGTTCT ATCAAGGGGGAAATCCGAAAGCAGGCACTGGATCACTTCAATGCAGATGGGTCTGAGGACTTCTGTTTCC TGCTCTCCACACGGGCTGGCGGCCTGGGAATCAATTTGGCTTCAGCGGACACAGTCGTCATCTTTGACTC TGACTGGAACCCGCAGAACGACTTGCAGGCACAAGCCCGAGCACATAGAATCGGTCAGAAGAAGCAGGTG AATATTTACCGCCTGGTTACAAAAGGGACTGTGGAAGAGGAGATCATAGAACGGGCCAAAAAGAAGATGG TGTTGGACCATCTGGTGATTCAGCGCATGGATACCACTGGCAGGACGGTTCTTGAAAACAACTCAGGAAG ATCCAACTCAAACCCTTTTAATAAAGAAGAGCTGACAGCTATTTTGAAATTTGGAGCAGAGGATCTCTTC AAAGAAATTGAAGGAGAGGAGTCAGAGCCTCAGGAAATGGATATAGATGAAATTTTGCGTTTAGCTGAAA CCAGAGAGAATGAAGTGTCAACTAGTGCGACAGATGAGCTGCTCTCACAGTTTAAGGTTGCCAACTTTGC AACAATGGAAGATGAAGAGGAGCTAGAAGAGCGTCCTCACAAAGACTGGGATGAAATTATTCCCGAAGAG CAAAGGAAAAAAGTGGAGGAGGAAGAACGGCAGAAGGAGCTGGAAGAAATTTATATGCTGCCTCGGATTC GCAGTTCCACTAAAAAGGCTCAGACAAACGACAGTGATTCAGATACTGAGTCTAAGAGGCAGGCCCAGAG GTCTTCTGCTTCTGAGAGTGAGACGGACGACTCTGATGATGACAAGAAGCCGAAGCGAAGAGGGCGCCCC CGAAGTGTGCGCAAAGACCTTGTGGAGGGCTTCACGGATGCCGAGATCAGAAGGTTCATCAAGGCTTATA AGAAGTTTGGTCTCCCTCTTGAAAGGCTAGAGTGCATAGCACGTGATGCTGAGCTAGTTGACAAGTCTGT GGCAGACTTGAAACGCCTGGGGGAGCTGATTCATAATAGCTGTGTGTCAGCAATGCAGGAATATGAGGAG CAACTGAAAGAAAGCACCAGTGAAGGGAAAGGACCAGGCAAGAGAAGAGGCCCAACAATCAAGATCTCTG GGGTTCAGGTGAACGTGAAGTCCATTATCCAACACGAAGAAGAGTTTGAGATGCTGCATAAATCTATCCC TGTGGACCCTGAGGAAAAAAAAAAGTACTGCCTAACCTGCCGAGTCAAAGCAGCACATTTTGACGTCGAG TGGGGCGTGGAGGACGATTCTCGTCTGTTGCTTGGAATCTATGAACATGGCTATGGAAATTGGGAGCTAA TTAAGACAGACCCAGAGCTAAAGCTAACTGATAAAATTCTACCAGTGGAGACGGATAAAAAGCCGCAGGG GAAGCAGCTGCAGACCCGAGTGGACTACTTGCTGAAGCTGCTCAGGAAGGGTCTGGAGAAGAAGGGCACC GTGGCCAGCGGAGAGGAGGCCAAACTAAAGAAGCGGAAGCCTCGAGTAAAGAAGGAGAACAAAGCCCCCA GGCTGAAGGACGAGCACGGGCTGGAGCCAGCGTCGCCTCGACACTCCGACAACCCATCTGAAGAGGGGGA AGTGAAGGATGATGGTCTAGAAAAAAGTCCAACGAAAAAAAAGCAGAAGAAGAAAGAGAACAAGGAGAAC AAAGAGAAACCAGTGAGCTCTCGGAAGGACAGGGAAGGAGACAAAGAGAGGAAGAAGTCCAAAGACAAGA AAGAGAAGGTCAAAGGTGGTGATGGCAAGTCTTCAAGTAAATCAAAGCGATCTCAGGGTCCTGTGCACAT TACAGCAGGAAGTGAGCCTGTTCCCATCGGGGAGGATGAAGACGATGATCTGGACCAGGAAACCTTCAGC ATTTGTAAGGAGAGGATGAGGCCTGTGAAGAAGGCACTGAAACAGTTGGACAAACCTGACAAAGGGCTCA GTGTGCAAGAACAGCTGGAACACACCAGGAACTGCCTGCTCAAAATTGGAGACCGCATAGCCGAGTGCCT TAAAGCTTACTCAGACCAGGAGCACATCAAATTGTGGAGGAGGAACCTATGGATTTTTGTTTCCAAGTTT ACAGAATTTGATGCACGAAAACTACATAAGTTATATAAGATGGCTCATAAGAAAAGATCTCAAGAAGAGG AGGAACAAAAGAAGAAAGATGATTCGCTTGGTGGTAAGAAACCATTTCGACCAGAGGCCTCGGGCTCAAG CCGGGATTCTCTGATATCTCAGTCCCATACCTCACACAACCTTCATCCTCAGAAGCCTCATTTGCCTGCC TCCCATGGCCCACAAATGCATGGACATCCAAGAGATAACTACAGTCACCCCAACAAGAGACACTTTAGTA ACGCAGATCGAGGAGACTGGCAGAGGGAAAGAAAGTTCAACTATGGTGGTGGTAACAGCGCCCCCTGGGG GGGCGACCGGCACCATCAGTATGAGCAGCATTGGTATAAGGACCACCACTATGGTGACCGGAGGCATATG GATGCCCACCGTTCTGGGAGCTACCGCCCTAACAACATGTCCAGAAAGAGGCCGTATGAGCAGTACAACA GCGACCGAGACCACCGGGGACACAGAGACTATTATGACAGGCACCATCATGACTCCAAGCGGAGGAGGTC AGATGACTTCAGGCCCCAGAATTATCACCAGCAGGATTTCCGACGAATGTCTGACCACAGACCCACCATG GGTTACCACGGCCAGGGACCCTCAGACCATTATCGCTCTTTCCACACAGACAAGTTGGGGGAATATAAGC AGCCCATGCCTTCATTGCACACTGCGCTCTCAGACCCACGCTCACCCCCTTCCCAGAAATCTCCCCATGA TTCCAAATCACCCTTGGATCATAGGTCTCCTTTGGAGAGATCACTAGAACAGAAAAACAACCCAGATTAT AACTGGAATGTTCGGAAAACATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001081345
Insert Size	5484 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001081345.2, NP_001074814.2
RefSeq Size	9071 bp
RefSeq ORF	5484 bp
Locus ID	244059
UniProt ID	E9PZM4
Gene Summary	DNA-binding helicase that specifically binds to the promoter of target genes, leading to chromatin remodeling, possibly by promoting deposition of histone H3.3. Involved in myogenesis via interaction with MYOD1: binds to myogenic gene regulatory sequences and mediates incorporation of histone H3.3 prior to the onset of myogenic gene expression, promoting their expression.[UniProtKB/Swiss-Prot Function]

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