Plxnb2 (NM_138749) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Debt; plexin-B2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224967 representing NM_138749 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCTGCCACTCTGGGCCCTGACCTTTCTAGGTCTCACGGGCCTAGGGTTGAGCCTGCGGTCCCGAA AGCCGGAGAGTTTCCGCAGTGAGACAGAGCTGAACCACCTGGCTGTGGATGAGGTCACAGGTGTGGTGTA TGTCGGGGCAGTGAACGCGCTCTACCAGCTGAGTGCCGACCTGCACGTCCAGCAGCACGTGGTCACAGGC CCCTTCATGGATAACAAGAAGTGCACACCACCCATTGAGGCCAGCCAGTGCCACGAAGCGGTGCTCACTG ACAACTTCAACCAGCTGCTGCTGCTCGACCCACCGGGGAAACGCCTGGTGGAGTGCGGCAGCCTCTTCAA GGGCATCTGTGCCCTGCGCGCCATGAGCAACATCTCGGTGCGCCTCTTCTACGAGGATGGCAGCGGCGAG AAGTCCTTCGTGGCCAGCAATGACGAGAGAGTGGCCACCGTGGGGCTGGTGACCTCCACGCGCCCCGACG GCGAGCGCGTGCTGTTTGTGGGCAAAGGCAATGGGCCGCACGACAATGGTATCATCGTGAGCACCCGCCT GCTGGACAGGGCTGAGGGCCGGGAGGCCTTTGAGGCCTACTCTGACCACACCACCTTCAAGGCCGGCTAC CTGTCCACGAATACTCAGCAGTTTGTCGCAGCCTTTGAGGATGATTTCTATGTCTTCTTCGTCTTTAACC ATCAAGACAAGCATCCTGCTAAGAACCGCACGCTGCTGGCGCGCATGTGCAAAGACGACCCCTCCTACTA TTCCTATGTAGAGATGGACCTGCAGTGTCAGGATCCCAGTGACCCCCAAGACTCTGCCTTTGGCACCTGC CTAGCCGCTTCTGTAGCCACATCGGGTGCTGGCAGAGCACTTTATGCTGTCTTCAGCAGAGATGGCCGGA GCACTGGGGGACCTGGCGCGGGCCTCTGTGTGTTCCCGCTAGATAAAGTCCGTGAGAAGATAGAAGCCAA CCGTAACGCCTGCTACACAGGTGCCCGAGAGGCAGGCCGCACTATCTTCTACAAGCCCTTCCACGGAGAA ATCCAGTGTGGCGGCCATCTGATAGGTGCCAGCGAGAGCTTCCCATGCGGCTCGGAGCATCTGCCCTATC CACTGGGCAGTCGTGATGGACTCGTCGCCACAGCCGTGCTGCACCGCGGGGGCCTGAATCTGACAGCAGT GACAGTAACTGCCGAGAATGACCACACTGTTGCGTTCCTGGGCACCTCAGATGGCCGGATCCTTAAGGTG TACCTTGCTCCAGACGGCACTTCTGCAGAGTATGGTTCTATCCCAGTAGACATCAATAAGAAAATCAAGC AGGACCTGGCACTGTCTGGAAACCTGTCCAGTCTGTATGCTATGACCCAGGACAAGGTGTTCCGGCTCCC AGTACAAGAATGCCTGAGCTATGTAACCTGCGCTCAGTGTCGTGACTCCCAAGATCCCTACTGTGGCTGG TGTGTCATCGAGGGACGATGCACCAGGAAGTCCGAGTGCTCACGGGCCGAGGAAACCGGACACTGGCTGT GGAGCCGGGAGAAGTCCTGTGTGGCCATCACTGATGCCTTTCCACAGAACATGAGCCGGCGGGCCCAAGG AGAGGTACGCCTGTCTGTCAGCCCCCTGCCCACCCTGACTGAGGACGATGAGTTACTGTGCCTCTTTGGT GACTCACCACCCCACCCTGCCCGGGTAGAGGACGATACCGTCATCTGTAATTCCCCAAGCAGCATCCCTA GTACACCGCCAGGCCAAGACCATGTTGACGTGAGCATCCAGCTCCTCTTAAAAAGCGGCAGTGTCTTCCT CACCTCCCACCAGTATCCCTTCTATGACTGCCGTGAGGCCATGAGCCTGGTGGAGAACCTGCCGTGCATC TCTTGTGCTAGCAACCGCTGGACTTGCCAATGGGACCTGCAGTACTACGAGTGTCGGGAGGCTTCGCCCA ACCCAGAGGAGGGAATCATACGTGCCCACATGGAGGACAACTGCCCCCAGTTCCTGGCCCCTGACCCTTT GGTCATCCCCATGAACCATGAGACAGAGGTGACCTTCCAGGGCAAGAACTTGGAGACTGTGAAGGTTTCT TCCCTGTATGTGGGCAGTGAGCTGCTGAATTTTGAAGAGACTGTGACCATGCATGAATCAGACACCTTCT CTTTTAGGACCCCAAAGCTATCCCATGATGGTAATGAGACACTGCCTCTTCACCTGTATGTTAAGTCCTT TGGCAAGAACATTGACAGCAAGCTACAAGTGACTCTCTACAACTGCTCCTTTGGCCGCAGTGACTGTAGC CTGTGTCTGGCTGCCGATCCTGCCTACAGGTGTGTGTGGTGCCGTGGGCAGAACCGGTGCGTGTACGAAG CTCTGTGCAGCAATGTCACTTCTGAGTGCCCACCACCAGTTATCACTAGGATCCAGCCTGAGACGGGCCC GCTGGGTGGGGGCATCCTGGTCACTATCCATGGGTCCAATCTGGGTGTCAAAGCAGATGACGTCAAGAAG ATAACTGTGGCTGGCCAGAACTGTGCCTTTGAACCAAGAGGGTACTCCGTATCCACCCGGATTGTGTGTG CAATTGAGGCTTCGGAGATGCCCTTCACAGGAGGCATTGAGGTGGATGTTAATGGAAAACTCGGCCATTC ACCGCCACACGTCCAGTTCACTTATCAACAACCCCAGCCTCTCAGTGTGGAGCCACGACAGGGGCCACAG GCAGGTGGCACCACATTGACCATCAATGGCACTCACCTGGACACAGGCTCCAAGGAGGATGTGCGGGTGA CACTCAATGACGTCCCTTGTGAAGTGACAAAGTTTGGAGCACAGCTACAGTGTGTCACAGGTCAACAGTT GGCTCCAGGCCAGGTGACACTAGAAATCTACTATGGGGGCTCCAGAGTGCCCAGCCCCGGCATCTCTTTC ACCTACTGCGAGAACCCCATGATACGAGCCTTTGAGCCATTGAGAAGCTTTGTCAGTGGTGGCCGGAGCA TCAACGTTACTGGCCAGGGCTTCAGCCTCATCCAGAAGTTTGCCATGGTTGTCATCGCTGAGCCCTTGCG GTCCTGGAGGCGGCGGCGGCGGGAGGCTGGAGCCCTGGAGCGTGTGACGGTCGAGGGCATGGAGTACGTG TTCTACAACGACACCAAGGTCGTCTTCTTGTCTCCTGCTGTCCCCGAAGAGCCCGAGGCTTACAACCTCA CCGTGCTGATACGGATGGACGGTCACTGTGCCCCGCTCAGGACCGAAGCCGGAGTCTTTGAGTATGTGGC AGACCCCACCTTTGAGAACTTCACGGGTGGCGTCAAAAAGCAGGTCAACAAGCTCATCCACGCCCGGGGA ACCAATCTGAACAAAGCCATGACTCTGGAGGAGGCAGAGGCATTTGTGGGTGCTGAGCGTTGCATTATGA AGACGCTGACCGAGACGGACCTGTACTGCGAGCCCCCAGAGGTGCAGCCTCCACCCAAGCGGCGGCAGAA GCGAGACACGGCACACAACCTACCTGAGTTCATTGTGAAGTTTGGCTCCCGAGAGTGGGTGCTAGGCCGA GTGGAGTACGACACGCGTGCGAGTGACGTGCCGCTCAGTCTCATCCTGCCTCTGGTCATGGTACCCATGG TGTTTATCATCGTGGTCTCCATCTACTGCTACTGGAGGAAGAGCCAGCAGGCCGAGCGCGAGTACGAGAA GATCAAATCCCAGCTCGAGGGCTTGGAGGAGAGCGTGCGTGACCGCTGCAAGAAGGAATTCACAGACCTG ATGATCGAGATGGAGGACCAGACGAATGACGTGCATGAGGCAGGCATCCCCACGCTGGACTACAAGACCT ACACCGACCGCGTATTCTTCCTGCCATCCAAGGATGGTGACAAGGATGTGATGATCACCGGCAAGCTAGA CATCCCTGAGTCACGGCGGCCCATTGTGGAGCAAGCCCTCTACCAGTTCTCCAACCTGCTTAATAGCAAG TCCTTCCTCATCAATTTCATCCACACCCTGGAGAATCAGCGTGAGTTCTCAGCTCGGGCCAAGGTCTACT TTGCATCACTGCTGACTGTGGCCCTGCACGGGAAGCTGGAGTACTACACGGACATCATGCGCACGCTCTT CCTGGAGCTCATGGAGCAGTATGTGGTGGCCAAGAACCCCAAGCTAATGCTGCGCAGGTCTGAGACAGTG GTGGAGAGGATGCTGTCCAACTGGATGTCCATCTGTCTGTACCAGTACCTCAAGGATAGTGCAGGGGAGC CTCTCTACAAGCTCTTCAAGGCCATCAAACACCAGGTGGAAAAGGGGCCCGTGGATGCTGTGCAGAAGAA GGCCAAGTACACCCTCAATGACACAGGCCTGCTCGGGGACGATGTTGAGTATGCGCCTCTGACCGTGAGC GTGATTGTTCAGGATGAAGGGATCGATGCCATCCCGGTTAAGGTCCTCAACTGTGACACCATCTCTCAGG TCAAAGAGAAGATCATCGACCAAGTGTACCGTACTCAGCCCTGCTCCTGCTGGCCCAAGCCAGACAGTGT GGTGCTTGAATGGCGTCCTGGGTCCACGGCCCAGATTCTGTCTGACTTGGACCTTACCTCCCAGCGGGAG GGCCGGTGGAAGCGCATCAACACCCTGATGCACTACAACGTCCGGGATGGAGCCACCCTCATCCTGTCTA AGGTGGGAGTCTCCCAGCAGCCAGAAGACAGCCAACAGGACTTGCCAGGGGAGCGCCATGCCCTTCTGGA AGAGGAAAACCGCGTGTGGCACTTGGTGCGGCCAACAGATGAGGTAGATGAAGGCAAGTCCAAGCGCGGC AGCATGAAGGAGAAAGAGCGGACTAAGGCCATCACAGAGATCTACCTGACGCGGCTACTCTCCGTCAAGG GCACACTGCAGCAGTTTGTGGACAACTTCTTCCAAAGCGTGCTGGCGCCTGGGCATGCGGTGCCACCCGC CGTCAAGTACTTCTTCGATTTCTTGGACGAGCAGGCAGAGAAGCATGACATCCGAGATGAGGATACCATC CATATCTGGAAAACCAACAGTTTACCACTTCGGTTCTGGGTGAACATCCTGAAGAACCCTCATTTCATCT TTGATGTCCACGTCCACGAAGTGGTGGACGCCTCCCTGTCAGTCATCGCACAGACCTTCATGGACGCCTG TACTCGCACGGAGCACAAGCTGAGCCGAGACTCTCCCAGCAACAAGCTGTTGTATGCTAAGGAGATCTCT ACCTACAAGAAGATGGTGGAGGACTACTACAAGGGCATCAGACAGATGGTGCAGGTCAGCGACCAGGACA TGAATACGCACTTGGCAGAGATTTCCCGGGCTCACACAGACTCCCTGAACACACTCGTGGCCCTACACCA GCTCTACCAATACACACAGAAGTACTATGATGAGATCATCAATGCTCTGGAGGAGGACCCTGCAGCCCAA AAGATGCAACTGGCCTTCCGCCTACAGCAGATTGCTGCTGCGCTTGAGAATAAGGTTACAGACCTCTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_138749
Insert Size	5529 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_138749.3, NP_620088.2
RefSeq Size	6519 bp
RefSeq ORF	5529 bp
Locus ID	140570
UniProt ID	B2RXS4
Gene Summary	Cell surface receptor for SEMA4C, SEMA4D and SEMA4G that plays an important role in cell-cell signaling (PubMed:17554007). Plays a role in glutamatergic synapse development and is required for SEMA4A-mediated excitatory synapse development (PubMed:29981480). Binding to class 4 semaphorins promotes downstream activation of RHOA and phosphorylation of ERBB2 at 'Tyr-1248' (PubMed:17554007). Required for normal differentiation and migration of neuronal cells during brain corticogenesis and for normal embryonic brain development (PubMed:19948886). Regulates the migration of cerebellar granule cells in the developing brain (PubMed:21122816). Plays a role in RHOA activation and subsequent changes of the actin cytoskeleton (By similarity). Plays a role in axon guidance, invasive growth and cell migration (By similarity). May modulate the activity of RAC1 and CDC42 (PubMed:21966369). Down-regulates macrophage migration in wound-healing assays (in vitro) (PubMed:21966369).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1-3 encode the same protein.

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