Dock5 (NM_177780) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1110060D06Rik; AI666732; AI956923; BC016533; E130320D18; lr2; rlc
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224989 representing NM_177780 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCGTTGGATCCCTACCAAGAGGCAGAAGTATGGGGTTGCCATCTACAACTACAATGCATCTCAAG ATGTGGAGCTCTCCTTGCAGATCGGCGACACCGTCCACATCCTGGAGATGTATGAGGGCTGGTACAGAGG ATACGCGCTCCAGAACAGATCTAAAAAGGGCATTTTTCCTGAGACATACATCCATTTGAAAGAGGCAACC GTGGAAGATGGAGGGCAGCATGAGACCGTGATTCCCGGGGAGTTGCCCCTGGTTCAGGAGCTCACAAACA CGCTACGAGAATGGGCCGTCATCTGGCGCAAGCTCTATGTGAACAACAAGGTCACACTATTCCGCCAGCT GCAGCAAATGACATACAGTCTGATCGAGTGGCGTTCCCAGATCCTATCTGGGACACTTCCCAAGGATGAA CTTGCAGAGCTGAAGAAGAAGGTCACAGCCAAAATCGATCATGGGAACAGAATGCTTGGACTGGATCTGG TTGTGCGGGACGACAATGGAAACATTTTAGATCCAGATGAGACCAGCACTGTCGCCCTATTCAGGGCCCA TGAAGTGGCTTCTAAAAGGATAGAGGAAAAGATCCAAGAGGAGAAGAGCATTTTGCAGAACCTGGACTTG AGAGGCCAGGCAATCTTCAGTACTGTCCACACCTATGGGCTCTATGTGAACTTCAAGAACTTTGTCTGTA ACATTGGGGAGGATGCGGAGCTGTTCATAGCCCTGTATGATCCAGACCAGTCCACCTTCATCAGTGAGAA CTACCTAATTCGCTGGGGCAGTAACGGGATGCCCAAGGAGATCGAGAAGCTGAATAACCTGCAAGCAGTG TTCACCGACCTCAGCAGCACAGACCTCATCCGGCCACGCATCAGCCTCGTGTGCCAGATTGTCCGGGTGG GCCGCATGGAGCTGAAGGAGGGCAAGAAGCACACCTGTGGGCTCCGGAGGCCCTTTGGAGTAGCAGTGAT GGATATTAGTGATATTGTACATGGGAAGGTGGATGATGAAGAAAAGCAACATTTTATCCCCTTTCAGCAA ATTGCCATGGAGACCTACATCCGCCAGAGACAGCTCATCATGTCCCCCTTGATAACCTCCCACGTGATCG GAGAGAATGAGCCACTCACGTCGGTCCTAAATAAAGTCATAGCTGCAAAGGAAGTGAATCACAAAGGACA AGGTCTTTGGGTGTCCTTAAAGTTACTGCCAGGTGACCTCACACAGGTGCAGAAGAACTTTTCACACCTG GTGGACAGATCAACAGCAATAGCACGGAAAATGGGCTTTCCAGAAATAATCCTGCCAGGGGATGTTCGAA ATGATATTTATGTCACCCTGATCCATGGGGAGTTTGACAAGGGGAAGAAGAAGACACCTAAAAACGTGGA GGTGACCATGTCTGTGTTTGATGAGGAAGGCAACCTTTTAGAGAAAGCCATTCATCCCGGTGCTGGGTAC GAAGGCGTCTCGGAGTACAAGTCAGTGGTGTATTACCAGGTCAAGCAACCCTGTTGGTACGAGACGGTCA AGGTCTTCATTGCCATTGAAGAGGTCACACGCTGCCACATACGATTTACCTTCCGACACAGATCATCTCA AGAATCCAGAGATAAATCAGAGCGAGCCTTTGGGGTGGCCTTTGTGAAACTGATGAACGCAGATGGTACC ACGCTGCAGGATGGGAGGCACGACCTGGTGGTGTACAAGGGTGATAATAAGAAAATGGAAGATGCTAAAT ATTATCTGACTCTGCCTGGGACCAAAGCAGAGCTGGAAGAAAAAGAACTCCAAGCGTCCAAAAACCCATC CGTCTTCACACCCAGCAAAGACAGCACGAAGGACAGCTTTCAGATCGCCACTCTCATCTGCTCCACAAAG CTCACCCAAAATGTGGACCTGCTAGGCTTACTAAACTGGCGTTCTAATTCTCAGAACATTAAGCACAACC TGAAAAAGTTAATGGAAGTGGATGGAGGAGAGATTGTTAAGTTTTTACAGGACACACTAGACGCACTTTT TAACATAATGATGGAAATGTCAGACAATGAAACATATGACTTCCTTGTGTTTGACGCTCTGGTCTTCATC ATTTCGCTGATAGGAGACATCAAGTTTCAGCATTTTAACCCTGTCCTCGAAACCTACATTTATAAGCACT TCAGCGCCACCTTGGCACACGTGAAACTCTCCAAGGTACTGAACTTTTATGTGGCCAATGCCGAGGACCC TAGCAAGACGGAATTGCTATTTGCTGCTTTGAAAGCCTTGAAGTACCTGTTCCGATTCATCATCCAGTCT CGGGTACTCTACTTGAGGTTTTATGGTCAGAGTGAAGATGGAGATGAATTTAACGACTCAATCCGCCAGC TGTTTCTTGCTTTCAACACGCTGATGGACAGGCCTCTGGAAGAAGCTGTCAAGATCAAGGGGGCAGCCTT GAAGTACCTTCCCAGCATAATTAATGATGTCAAACTGGTGTTTGATCCCATGGAGCTCAGCGTGCTCTTC TGCAAGTTCATTCAAAGCATCCCTGACAACCAGTTGGTGCGGCAGAAACTTAACTGCATGACCAAGATAG TGGAGAGCAGCCTTTTCCAGCAGGCAGAGTGCAGGGAAGTGCTGCTGCCGCTACTGACAGACCAGCTCAG TGGCCAGCTGGACGACCACTCAACCAAACCGGACCACGAGGCAAGCTCACAGCTTCTAAGCAACATCCTA GAGGTGCTAGACAGGACGGATGTGGGCCCCACTTCAGCTCACGTACAGCTGATCATGGAGAGGCTGCTGA GAAGGATCAACCGGACCGTGATTGGGATGAGCCGACAGTCTCCTCACATTGGCAGCTTTGTGGCTTGCAT GATTGCCGTCTTACGACAAATGGAGGACAGCCACTACAGCCACTACATCAGCACCTTCAAAACCAGGCAG GACATCATTGACTTTCTCATGGAAACCTTCATTATGTTTAAGGATCTGATTGGAAAGAATGTGTATGCCA AAGATTGGATGGTCATGAACATGACCCAGAACAGAGTTTTCCTCCGTGCCATCAATCAGTTTGCTGAGGT TCTCACCAAGAGCTTCATGGACCAGGCAAGCTTTGAACTTCAGCTCTGGAACAATTACTTCCATTTGGCT GTAGCTTTTCTCACCCACGAGTCCCTCCAGCTTGAAACCTTCTCAGAAGCCAAGCGCAACAAAATTGTGA AAAAATATGGGGACATGAGAAAAGAAATTGGCTTCAGGATCCGGGACATGTGGTATAACCTGGGGCCACA CAAAATCAAATTCATCCCATCCATGGTGGGTCCCATTCTGGAAGTCACTCTGACCCCTGAAGTGGAGCTC CGTAAAGCCACGATCCCCATTTTCTTTGATATGATGCAGTGCGAGTTCAATTTGAGTGGAAATGGCAACT TCCACATGTTTGAGAATGAGTTGATCACAAAGCTGGATCAGGAAGTGGAAGGTGGCCGAGGAGACGAACA ATACAAGGTTCTTCTGGAAAAGCTACTCTTAGAGCATTGCCGGAAACATAAATACCTCGCGAACTCTGGG GAAGCCTTTGCCTTCCTGGTCAGCAGCCTCTTGGAGAACCTGCTGGATTACAGAACGATCATCATACATG ATGAGAGCAAGGAGAACCGCATGAGCTGTACAGTGAACGTCCTGAATTTCTATAAAGATAAGAAACGAGA GGACATATACATACGGTATTTATACAAGCTTCGGGATTTGCACAGAGACTGTGAGAACTACACAGAAGCT GCCTATACACTCCTCCTACATGCTGAGCTTCTGCAGTGGTCGGACAAGCCCTGTGTGCCCCATTTGCTGC AGAGGGATAGTTACTATGTTTATACCCAGCAGGAGCTTAAAGAGAAACTGTATCAAGAGATCATATCCTA TTTTGATAAAGGCAAAATGTGGGAGAAAGCCATCAAACTGAGCAAGGAGTTGGCGGAGACCTACGAGAGC AAAGTGTTTGACTACGAGGGCCTAGGCAGTCTCTTGAAAAAAAGAGCCTTGTTTTACGAGAACATCATTA AGGCCATGAGGCCCCAGCCTGAATACTTTGCTGTGGGATACTACGGACAGGGCTTCCCTTCCTTCCTGCG GAACAAAATCTTTATCTACCGGGGTAAGGAGTATGAGAGGCGGGAAGACTTCAGCTTGAGGTTGCTGACC CAGTTCCCCAATGCTGAGAAGATGACCAGTACCACACCACCAGGAGAAGACATCAAGTCATCCCCAAAGC AGTACCTGCAGTGCTTCACGGTAAAACCTGTGATGAGCTTGCCGCCCAGCTACAAGGACAAACCGGTTCC AGAGCAGATCTTGAACTACTACAGAGCCAACGAAGTGCAGCAGTTCAGCTATTCCCGGCCATTCCGGAAA GGAGAAAAAGACCCAGAAAATGAGTTTGCCACCATGTGGATTGAAAGAACCACGTACAGAACGGCCTATA CCTTCCCTGGGATCCTCAAGTGGTTCGAAGCCAAGGAGATTTCAGTAGAAGAAATCAGCCCTTTGGAGAA TGCCATAGAAACCATGGAGCTGACCAACGAGAGGGTCAGCAACTGCGTGCAGCAGCACGCCTGGGACCAT TCTCTCTCTGTGCACCCGCTCTCCATGCTGCTCAGCGGCATAGTGGACCCAGCTGTCATGGGGGGATTCT CCAACTACGAAAAGGCTTTTTTTACAGAAAAGTACTTACAAGAGCATCCCGAGGACCAGGAGAAGGTGGA GCTGCTCAAGCGACTGATTGCCTTACAAATACCTCTGCTTACGGAAGGGATCCGCATCCACGGGGAGAAG CTGACGGAGCAGCTCAAGCCGCTGCATGCCCGGCTATCTTCGTGCTTCCGTGAACTCAAGGAGAAAGTAG AGAAGCTGTATGGAGTCATAACGCTGCCACCCAGCATGACAGAGAGGAAGCCAAGCCGTGCAGGCTCGAT GGTACTGCCCTACATCCTGTCCTCCACGCTGCGCAGGCTGTCTGTCACCTCTGTGGCCTCCTCGGTGATC TCCACCTCCTCCAACTCTTCTGACAATGCATCGTCCAGGCCAGGATCGGATGGGTCCATCCTGGAACCCC TGTTTGAACGCAGAGCTTCATCGGGTGCCAGAGTTGAAGATCTGCCACCCAAAGAAGACAGTGAGAACCG GATTAGCAAGTTCAAGAGGAAAGACTGGAATCTGAGCAAATCACAAGTCATTGCAGAGAAGGCTCCAGAA CCTGATGTGATGAGCCCAGGCAAAAAAACACAAAGGCCAAAGAGTCTCCAGTTGGTGGACAGCCGGCTGA CACCATTCCACAGTCCCTCGCCTCTGCAGTCTACAGCCTTGAGCCCACCCCCACTCACTCCCAAAGCCAC GAGAACCCTAAGCTCCCCATCTTTGCAGACAGATGGGCTGACGGCTTCTGTCCCTCCTCCACCTCCCCCC AAAAGCAAACCGTATGAGAGCAGTCAGAGGAACTCGGCTGAGATTGCTCCCCCACTGCCTGTCCGGAGAG ACTCCAAAGCCCCACCTCCACCCCCTCCAAAGGCTCGGAAATCAGGCATCCTCTCTTCTGAGCCAGGCTC TCAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_177780
Insert Size	5607 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_177780.3, NP_808448.2
RefSeq Size	10335 bp
RefSeq ORF	5607 bp
Locus ID	68813
UniProt ID	B2RY04
Gene Summary	Guanine nucleotide exchange factor (GEF) for Rho and Rac. GEF proteins activate small GTPases by exchanging bound GDP for free GTP (PubMed:18396277). Along with DOCK1, mediates CRK/CRKL regulation of epithelial and endothelial cell spreading and migration on type IV collagen (By similarity).[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.