Ptprf (NM_011213) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AA591035; LAR; LARS; RPTP-LAR
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225019 representing NM_011213 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCCCGAGCCAGCCCCAGGGAGGAGGATGGTGCCCCTTGTGCCTGCTCTCGTGATGCTTGGTTTGA TGGCAGGTGCTCATGGCGACAGCAAACCCGTCTTTGTTAAGGTCCCCGAGGACCAGACTGGGCTGTCGGG AGGGGTGGCCTCCTTCGTGTGCCAAGCCACAGGGGAACCCAAGCCTCGAATTACGTGGATGAAGAAGGGG AAGAAAGTCAGCTCCCAGCGCTTTGAGGTAATTGAGTTTGACGATGGAGCAGGGTCAGTGCTGCGGATCC AGCCATTACGAGTGCAGCGAGACGAAGCCATCTATGAGTGCACAGCCACGAACAGTCTCGGGGAGATCAA CACAAGTGCCAAGCTGTCAGTGCTTGAAGAGGACCAGCTGCCGTCTGGGTTCCCGACTATCGACATGGGA CCTCAGCTGAAGGTGGTGGAGAAGGGTCGCACTGCCACCATGCTGTGTGCAGCCGGTGGGAACCCAGACC CTGAGATCTCTTGGTTCAAAGACTTCCTTCCTGTGGACCCTGCTGCAAGCAACGGTCGTATCAAACAGCT GCGATCAGGTGCATTGCAGATAGAGAGCAGCGAGGAGTCTGACCAAGGCAAGTACGAGTGTGTGGCCACC AACTCTGCAGGCACACGCTACTCGGCCCCCGCCAACCTGTATGTGCGAGTGCGTCGCGTGGCTCCTCGTT TCTCCATCCCTCCCAGCAGCCAAGAGGTGATGCCGGGCGGCAGCGTGAATCTCACATGTGTGGCAGTGGG CGCGCCCATGCCGTATGTGAAATGGATGATGGGCGCCGAGGAACTGACCAAAGAGGATGAGATGCCAGTC GGCCGAAATGTTCTGGAGCTCAGCAATGTCATGCGATCTGCCAACTATACCTGTGTGGCCATCTCTTCAT TAGGCATGATAGAAGCCACGGCCCAGGTCACAGTAAAAGCTCTGCCAAAGCCTCCAATTGATCTTGTGGT GACAGAGACAACCGCCACCAGTGTTACTCTGACATGGGACTCTGGAAATACCGAGCCTGTGTCCTTCTAC GGCATCCAGTACCGCGCAGCTGGCACAGACGGCCCCTTCCAGGAAGTGGATGGTGTGGCCAGCACCCGAT ACAGCATTGGTGGCCTCAGCCCCTTTTCGGAATATGCTTTCCGTGTGTTGGCTGTTAACAGCATTGGGCG AGGACCACCCAGTGAGGCAGTGCGAGCACGCACGGGGGAGCAGGCACCCTCCAGCCCTCCGCGCCGTGTG CAGGCTCGAATGCTTAGCGCCAGCACCATGCTGGTCCAGTGGGAACCACCTGAAGAGCCCAATGGCCTGG TACGGGGATATCGCGTCTACTATACCCCAGATTCCCGACGCCCTTTGAGCGCCTGGCACAAGCATAACAC TGACGCAGGACTCCTTACCACCGTGGGCAGCCTGCTACCTGGCATCACCTACAGCCTCCGAGTCCTTGCC TTCACTGCTGTGGGTGACGGCCCACCCAGCCCCACCATCCAAGTCAAGACACAGCAGGGAGTGCCTGCAC AGCCTGCGGACTTCCAGGCTAACGCTGAGTCGGACACCAGGATCCAGCTCTCGTGGCTGCTGCCCCCGCA GGAGCGGATCGTCAAGTATGAGCTGGTGTACTGGGCAGCCGAGGATGAGGGCCAGCAGCACAAGGTGACC TTCGACCCCACCTCCTCCTACACTCTGGAGGACCTGAAGCCTGACACATTGTACCACTTCCAGCTGGCTG CCCGTTCAGATTTGGGGGTGGGCGTCTTCACGCCCACCGTAGAGGCCCGTACAGCACAGTCCACCCCATC AGCCCCTCCCCAGAAGGTGACATGTGTGAGCACGGGCTCCACCACGGTCCGGGTAAGTTGGGTTCCACCG CCGGCCGACAGCCGCAACGGCATTATCACCCAGTACTCCGTGGCCTATGAGGCAGTGGACGGCGAAGACC GTAAGCGACATGTGGTGGATGGCATCAGCCGTGAGCATTCCAGCTGGGACCTGCTGGGCCTGGAGAAGTG GACGGAGTACCGGGTGTGGGTGCGGGCACACACAGATGTGGGCCCTGGCCCTGAGAGCAGCCCGGTGCTG GTGCGCACCGATGAGGACGTGCCTAGCGGGCCACCACGGAAGGTAGAGGTTGAGCCTCTGAACTCCACTG CTGTGCATGTCTCCTGGAAGCTGCCCGTCCCCAACAAGCAGCACGGACAGATTCGTGGCTACCAGGTCAC CTATGTGCGGTTGGAGAATGGTGAGCCCCGAGGCCAACCCATCATCCAGGATGTCATGCTGGCTGAGGCT CAGGAGACCACCATCAGCGGCCTCACCCCCGAGACCACCTACTCCATCACCGTCGCTGCCTACACCACTA AGGGGGATGGTGCCCGAAGCAAGCCCAAGGTGGTTACTACAACAGGGGCAGTCCCAGGACGGCCCACCAT GATGGTCAGCACCACGGCCATGCACACCGCGCTGCTGCAGTGGCACCCACCCAAGGAGCTGCCTGGAGAG CTGCTGGGCTACCGTCTCCAATACCGGCGGGCCGACGAGGCGCGGCCCAACACCATAGACTTTGGCAAGG ATGACCAACATTTTACAGTCACCGGCCTGCACAAAGGGGCCACCTACGTCTTCCGGCTCGCTGCCAAGAA CCGGGCTGGCCCAGGAGAAGAGTTTGAGAAAGAGATCACCACCCCCGAGGATGTGCCCAGTGGCTTTCCT CAAAATCTTCGAGTGACAGGACTGACCACATCTACCACGGAACTGACCTGGGACCCGCCGGTGCTGGCGG AGAGGAATGGTCACATCACCAACTATACTGTGGTCTACCGTGACATCAACAGCCAGCTTGAACTACAGAA TGTCACCAACGACACACACCTTACACTCTTGGGCCTTAAACCGGACACCACTTATGACATCAAGGTCCGT GCACATACCAGCAAAGGCGCCGGCCCTCTCAGCCCCAGCATCCAGTCCCGGACCATGCCCGTGGAGCAAG TGTTTGCCAAGAATTTCCGTGTGGCCGCTGCGATGAAGACATCTGTGCTGCTCAGTTGGGAGGTCCCCGA CTCTTATAAGTCAGCTGTGCCCTTCAAGATCCTGTACAATGGGCAGAGCGTGGAGGTGGATGGGCACTCG ATGCGGAAGCTGATTGCAGACCTGCAACCCAACACGGAGTACTCCTTCGTCCTGATGAATCGTGGCAGTA GCGCCGGGGGCCTACAGCACCTGGTGTCCATCCGCACTGCCCCGGACCTCCTACCCCAGAAGCCACTGCC TGCCTCCGCCTTTATAGAGGATGGCCGCTTCTCCCTCTCCATGCCTCAAGTGCAGGACCCCTCGCTAGTC AGGTGGTTCTACATTGTGGTGGTGCCCATTGACCGTGTGGGCGGGAACTTGCTGGCACCAAGATGGAACA CACCAGAGGAGTTGGAGCTGGACGAGCTTCTGGAGGCCATCGAGCAGGGCGAGGAGAAACAGCGGAGGCG CCGGCGCCAAGCAGAGCGGCTGAAGCCTTATGTGGCGGCCCAAGTGGATGTGCTCCCTGACACCTTCACC CTGGGGGACAAGAAGAGCTACCGCGGCTTCTACAACCGGCCCCTGTCTCCGGATCTGAGTTACCAGTGCT TCGTTCTCGCCTCCCTCAAGGAACCCATGGACCAGAAGCGCTACGCCTCCAGCCCCTACTCGGACGAGAT TGTAGTCCAGGTGACGCCAGCACAGCAGCAGGAGGAGCCCGAGATGCTGTGGGTGACAGGCCCTGTCCTG GCGGTCATTCTCATCATACTCATTGTCATCGCCATCCTCCTGTTCAAGAGGAAGAGAACACACTCCCCAT CATCAAAGGATGAGCAGTCAATCGGGCTGAAGGACTCCCTGTTGGCCCACTCTTCTGACCCTGTGGAGAT GCGAAGGCTTAACTACCAGACCCCAGGTATGCGAGACCACCCGCCCATCCCCATCACTGACCTGGCAGAC AATATTGAGCGCCTCAAAGCCAACGATGGGCTCAAGTTCTCCCAGGAGTATGAGTCCATTGACCCTGGAC AGCAGTTCACATGGGAGAATTCCAACTCGGAGGTGAACAAGCCCAAGAACCGCTATGCAAATGTCATTGC CTATGACCATTCTCGAGTCCTCCTCACCTCCATTGATGGTGTTCCTGGGAGTGACTACATCAATGCCAAC TACATTGATGGCTACCGAAAGCAGAATGCCTACATCGCCACACAGGGTCCGCTGCCCGAGACCATGGGCG ATTTCTGGAGGATGGTGTGGGAACAGCGCACAGCCACAGTGGTCATGATGACCAGGCTAGAGGAGAAATC CCGGGTGAAGTGTGATCAGTATTGGCCAGTCCGTGGCACTGAGACCTATGGCCTCATTCAGGTGACCCTG GTGGACACTGTGGAGTTGGCCACATACACCATGCGCACCTTTGCCCTCCATAAGAGTGGCTCCAGTGAGA AGCGTGAGCTGCGTCAGTTCCAGTTCATGGCCTGGCCAGACCACGGCGTTCCTGAGTACCCCACTCCCAT CTTGGCCTTCCTGAGACGGGTCAAGGCCTGTAACCCACTAGATGCGGGGCCCATGGTGGTGCATTGCAGT GCGGGTGTGGGGCGCACAGGCTGCTTCATCGTCATCGACGCAATGCTGGAGCGTATGAAGCACGAGAAGA CGGTTGACATCTATGGCCACGTGACGTGCATGCGCTCACAAAGGAACTACATGGTGCAGACCGAGGACCA GTATGTGTTCATCCACGAGGCCCTGCTAGAGGCTGCCATGTGCGGACACACCGAGGTGCTCGCTCGGAAC CTCTATGCCCACATCCAGAAGCTAGGCCAAGTGCCTCCCGGGGAGAGCGTCACGGCCATGGAACTTGAGT TCAAGTTGCTGGCCAACTCCAAGGCCCACACGTCGCGCTTTGTCAGTGCCAACCTGCCCTGCAACAAGTT CAAGAACCGCCTAGTGAACATCATGCCCTATGAGCTGACCCGAGTGTGCTTGCAACCCATCCGTGGTGTG GAGGGCTCAGACTACATCAATGCCAGCTTTCTAGATGGCTACAGACAGCAGAAGGCCTACATAGCTACAC AGGGGCCTCTGGCAGAGAGCACCGAGGACTTCTGGCGCATGTTATGGGAGCACAATTCCACCATCATCGT CATGCTGACCAAGCTTCGGGAGATGGGCAGGGAGAAATGTCACCAGTACTGGCCAGCAGAGCGCTCCGCT CGCTATCAGTACTTCGTTGTTGACCCGATGGCTGAGTACAACATGCCCCAGTATATTCTGCGTGAATTCA AAGTCACAGACGCCCGGGATGGGCAGTCAAGGACAATCCGACAGTTCCAGTTTACAGACTGGCCAGAGCA AGGAGTACCCAAAACAGGTGAAGGCTTCATCGACTTCATCGGGCAGGTGCACAAGACAAAGGAGCAGTTT GGCCAGGATGGGCCCATCACGGTGCACTGCAGTGCTGGTGTGGGCCGCACCGGTGTGTTCATCACCCTGA GCATTGTCCTGGAGCGCATGCGGTATGAGGGTGTGGTTGACATGTTCCAGACCGTGAAGACCCTCCGCAC ACAGCGCCCTGCAATGGTGCAGACAGAGGACCAATACCAGCTGTGCTACCGTGCGGCCCTGGAATACCTC GGCAGCTTTGATCACTATGCAACGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_011213
Insert Size	5697 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_011213.2, NP_035343.2
RefSeq Size	7648 bp
RefSeq ORF	5697 bp
Locus ID	19268
UniProt ID	A2A8L5
Gene Summary	Possible cell adhesion receptor. It possesses an intrinsic protein tyrosine phosphatase activity (PTPase) and dephosphorylates EPHA2 regulating its activity (By similarity).[UniProtKB/Swiss-Prot Function]

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