Dicer1 (NM_148948) Mouse Untagged Clone

CAT#: MC225027

Dicer1 (untagged) - Mouse Dicer1, Dcr-1 homolog (Drosophila) (Dicer1), (10ug)



  "NM_148948" in other vectors (2)

CNY 19,224.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 1110006F08Rik; D12Ertd7e; mKIAA0928
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC225027 representing NM_148948
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGGCCTGCAGCTCATGACCCCTGCTTCCTCACCAATGGGTCCTTTCTTTGGACTGCCATGGCAAC
AAGAAGCAATTCATGATAACATTTATACGCCAAGAAAATACCAGGTTGAGCTGCTGGAAGCAGCTCTGGA
CCATAACACAATTGTGTGCTTGAACACTGGCTCAGGGAAGACGTTCATCGCGGTCCTGCTCACCAAAGAG
CTGGCCCATCAGATCAGGGGCGACCTCAACCCGCATGCAAAAAGGACCGTGTTCCTCGTCAACTCTGCAA
ACCAGGTTGCTCAACAAGTGTCAGCCGTCAGAACTCACTCGGATCTCAAGGTTGGAGAGTACTCAGACCT
AGAAGTAAATGCATCTTGGACAAAAGAGAGATGGAGCCAAGAGTTTACTAAGCACCAGGTTCTCATTATG
ACTTGCTATGTCGCCTTGACTGTTTTGAAAAATGGTTACTTATCACTGTCAGACATTAACCTTTTGGTGT
TTGATGAGTGTCATCTTGCGATTCTAGACCACCCCTATCGTGAAATTATGAAGCTCTGTGAGAGTTGTCC
ATCATGCCCTCGAATTTTGGGACTAACTGCTTCCATTTTAAATGGGAAATGTGATCCAGAGGAATTGGAA
GAGAAGATTCAGAAACTGGAGCGAATTCTCAGGAGTGACGCTGAGACGGCGACTGACTTGGTGGTCCTGG
ACAGATACACTTCTCAGCCTTGTGAGATTGTGGTAGACTGTGGACCGTTTACTGACAGAAGTGGGCTGTA
TGAGAGATTGCTGATGGAGTTAGAAGCAGCACTTGATTTTATCAATGATTGTAATGTAGCTGTACATTCA
AAGGAAAGAGATTCTACTTTGATTTCCAAACAAATCCTCTCAGACTGCCGTGCAGTGTTGGTTGTTCTGG
GACCCTGGTGTGCAGATAAAGTAGCTGGGATGATGGTAAGAGAACTTCAGAAGTACATCAAACATGAGCA
AGAGGAGCTACACAGGAAGTTCCTATTGTTTACAGACACCTTGTTAAGGAAAATACACGCCCTGTGTGAA
GAGTACTTCTCACCTGCCTCACTTGACCTGAAGTATGTAACTCCCAAAGTCATGAAACTGCTCGAGATCC
TGCGCAAATACAAGCCCTATGAGCGACAGCAGTTTGAAAGTGTTGAGTGGTACAATAACAGGAATCAGGA
TAATTACGTGTCCTGGAGTGATTCCGAGGATGACGATGATGACGAAGAAATTGAAGAAAAAGAAAAGCCA
GAGACAAATTTTCCTTCTCCGTTTACCAATATTTTATGTGGGATTATTTTTGTGGAAAGAAGATACACAG
CAGTTGTCCTAAACAGATTGATAAAGGAAGCTGGCAAACAAGATCCAGAGCTGGCTTATATCAGCAGCAA
CTTTATAACTGGACACGGCATTGGAAAGAACCAGCCTCGCAGCAAACAGATGGAGGCGGAGTTCAGAAAG
CAGGAGGAGGTACTTAGGAAATTTCGAGCTCATGAGACCAACCTGCTCATTGCAACAAGTGTTGTAGAAG
AGGGTGTGGATATTCCCAAGTGCAATTTGGTGGTTCGTTTTGATTTGCCTACAGAGTATCGATCATATGT
CCAGTCTAAAGGACGGGCACGAGCGCCGATCTCTAATTACGTAATGTTAGCAGATACAGACAAGATAAAG
AGCTTTGAAGAAGACCTCAAGACCTACAAAGCTATTGAAAAGATCTTAAGGAACAAGTGTTCGAAGTCAG
CGGATGGTGCTGAGGCGGACGTCCATGCTGGTGTAGATGATGAGGATGCTTTCCCGCCATATGTGCTGAG
GCCTGATGATGGGGGTCCTCGAGTCACAATCAACACTGCCATTGGACACATCAACAGATATTGTGCTCGA
TTACCAAGTGATCCGTTTACGCATCTAGCTCCCAAATGTAGAACCCGAGAATTGCCTGATGGTACATTTT
ATTCAACTCTTTATCTTCCGATTAACTCACCTCTTCGAGCCTCCATTGTTGGTCCACCGATGGACAGTGT
ACGGTTGGCTGAAAGAGTGGTAGCTCTCATTTGCTGTGAAAAACTGCACAAAATTGGTGAACTGGATGAA
CACTTGATGCCAGTTGGGAAAGAGACTGTTAAATATGAAGAAGAGCTGGACTTACATGACGAGGAGGAGA
CCAGTGTTCCCGGGAGACCAGGGTCCACAAAACGACGGCAGTGCTACCCCAAAGCAATTCCAGAGTGCTT
GAGGGAGAGTTACCCCAAACCCGACCAGCCTTGTTACCTGTATGTGATAGGAATGGTCTTAACTACCCCC
CTCCCTGATGAACTCAACTTCAGGCGGCGGAAGCTCTACCCACCTGAGGACACCACAAGATGCTTTGGGA
TCCTGACAGCCAAACCCATACCTCAGATTCCTCACTTTCCTGTGTATACACGCTCTGGAGAGGTCACCAT
ATCCATCGAGCTGAAGAAGTCTGGTTTCACATTGTCTCAACAAATGCTCGAGCTGATTACAAGATTGCAC
CAGTACATATTCTCACATATTCTCCGGCTTGAGAAGCCTGCCCTGGAGTTTAAACCTACAGGCGCGGAGT
CAGCCTACTGTGTTCTACCTCTTAATGTTGTTAATGACTCCGGCACTTTGGACATTGACTTTAAATTCAT
GGAGGATATTGAGAAGTCGGAAGCTCGGATAGGCATTCCTAGCACCAAGTATTCAAAGGAAACACCGTTT
GTCTTTAAGTTAGAAGATTACCAAGATGCAGTGATCATTCCAAGATACCGCAATTTTGATCAGCCTCATC
GATTTTATGTAGCTGATGTATATACGGATCTTACCCCACTCAGTAAATTTCCTTCCCCTGAATATGAAAC
TTTTGCAGAATATTATAAAACAAAGTATAACCTTGACCTGACCAATCTCAACCAGCCACTGCTGGATGTT
GACCACACATCTTCCAGACTGAACCTTTTGACACCTCGGCATTTGAATCAGAAGGGGAAAGCACTTCCTC
TAAGCAGTGCTGAGAAGAGGAAGGCCAAATGGGAAAGTCTGCAGAACAAACAGATCCTGGTTCCGGAACT
CTGTGCTATACATCCAATCCCAGCATCACTGTGGAGAAAAGCGGTTTGTCTCCCCAGCATACTGTATCGC
CTTCACTGCCTTCTGACTGCAGAGGAACTAAGAGCTCAGACAGCCAGCGATGCTGGTGTGGGTGTCAGAT
CACTTCCCGTGGATTTTAGATACCCTAACTTAGACTTCGGGTGGAAAAAATCTATTGATAGCAAGTCTTT
CATCTCAAGCTGTAACTCCTCGTTGGCTGAGAGTGATAATTACTGTAAGCACAGCACAACTGTAGTCCCT
GAACATGCTGCACATCAAGGTGCTACTAGACCCTCCTTAGAAAACCATGACCAAATGTCTGTGAACTGCA
AAAGGTTGCCCGCTGAGTCACCTGCTAAGCTCCAAAGTGAGGTTTCAACGGATCTTACAGCAATTAATGG
TCTTTCTTACAATAAAAACCTTGCCAATGGCAGTTATGATTTAGTTAACAGAGACTTTTGCCAAGGAAAT
CAGCTGAATTACTTCAAGCAGGAAATACCTGTACAACCAACTACCTCATATCCCATTCAGAATTTGTACA
ATTATGAGAACCAGCCCAAGCCCAGCAATGAATGTCCTCTACTGAGTAATACGTACCTTGATGGGAACGC
TAACACATCTACCTCAGATGGAAGCCCCGCGGTGTCCACGATGCCTGCCATGATGAATGCTGTTAAAGCA
CTCAAGGACAGGATGGACTCTGAGCAGAGCCCTTCTGTTGGGTACTCCTCAAGGACTCTTGGCCCCAATC
CGGGACTGATTCTCCAGGCTTTGACACTGTCAAATGCCAGTGATGGGTTTAACCTGGAGCGGCTTGAGAT
GCTTGGCGACTCCTTTTTAAAGCATGCTATCACCACATATCTGTTTTGCACGTACCCTGATGCTCATGAA
GGCCGCCTTTCATATATGAGAAGCAAAAAGGTCAGCAACTGTAACCTGTATCGCCTTGGCAAGAAGAAGG
GACTGCCCAGCCGCATGGTGGTGTCGATATTCGATCCTCCTGTGAATTGGCTTCCTCCTGGTTATGTGGT
AAACCAAGACAAAAGTAACTCAGAGAAGTGGGAAAAGGATGAAATGACAAAAGACTGCTTGCTGGCTAAT
GGCAAACTGGGTGAGGCCTGTGAGGAGGAGGAGGACCTGACGTGGAGGGCCCCCAAGGAGGAGGCCGAGG
ATGAGGACGACTTCCTGGAGTATGACCAGGAGCATATCCAGTTTATAGACAGCATGCTCATGGGCTCGGG
CGCCTTCGTGAGGAAAATCTCTCTGTCGCCCTTCTCAGCCTCCGACTCTGCATACGAATGGAAGATGCCC
AAGAAGGCCTCCTTGGGCAGTATGCCGTTTGCCTCGGGCCTTGAGGATTTTGATTACAGCTCTTGGGATG
CGATGTGCTATCTGGATCCTAGCAAAGCCGTTGAAGAGGATGACTTTGTGGTAGGTTTCTGGAATCCATC
AGAAGAGAACTGTGGCGTCGACACAGGGAAGCAGTCCATTTCTTACGACTTGCACACGGAGCAGTGCATT
GCTGACAAGAGCATAGCGGACTGTGTGGAGGCCCTGCTGGGCTGCTACTTAACCAGCTGCGGCGAGAGGG
CTGCTCAGCTCTTCCTCTGTTCGCTGGGGCTGAAGGTGCTCCCAGTAATCAAAAGGACCAGCCGGGAGAA
GGCCTTGGACCCTGCTCAGGAGAACGGAAGCAGCCAACAAAAGAGCCTTTCAGGGAGCTGTGCCTCTCCA
GTTGGCCCACGCTCCTCTGCAGGGAAAGACTTGGAGTACGGCTGCCTGAAGATCCCACCGAGGTGCATGT
TTGACCATCCAGACGCAGAGAAAACCCTCAACCACCTGATATCTGGGTTTGAAACTTTTGAGAAGAAAAT
TAACTATAGATTCAAGAATAAGGCTTATCTTCTGCAGGCTTTTACACACGCCTCCTACCACTACAACACT
ATCACTGATTGTTACCAGCGCTTAGAATTCCTGGGAGATGCGATTTTGGACTACCTCATAACCAAGCACC
TTTATGAAGACCCACGGCAGCATTCTCCAGGGGTCTTGACTGACTTGCGCTCTGCCCTTGTCAATAACAC
CATCTTTGCATCATTGGCTGTGAAGTATGACTACCACAAGTACTTTAAAGCTGTCTCTCCTGAGCTCTTT
CATGTTATTGATGACTTTGTGAAGTTTCAGCTTGAGAAGAACGAAATGCAAGGAATGGACTCTGAGCTTA
GGAGATCCGAGGAGGATGAGGAGAAAGAAGAGGATATCGAAGTCCCCAAGGCCATGGGGGACATTTTTGA
GTCTCTTGCTGGTGCCATTTATATGGACAGTGGGATGTCTCTGGAGGTGGTCTGGCAGGTGTACTATCCG
ATGATGCAGCCTCTAATAGAAAAGTTTTCTGCAAATGTGCCTCGTTCTCCAGTGAGAGAATTGCTCGAGA
TGGAACCAGAAACTGCCAAGTTTAGCCCAGCGGAGAGAACTTATGATGGAAAGGTCAGAGTCACCGTGGA
AGTGGTGGGAAAGGGGAAATTTAAAGGTGTTGGCCGGAGCTACAGGATCGCCAAGTCTGCTGCAGCACGA
AGGGCCCTCCGCAGCCTCAAAGCTAACCAGCCTCAGGTTCCTAACAGCTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI     
ACCN NM_148948
Insert Size 5721 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_148948.2, NP_683750.2
RefSeq Size 9851 bp
RefSeq ORF 5721 bp
Locus ID 192119
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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