Nckap5 (NM_001081756) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	8430408F21; D130011D22Rik; E030049G20Rik; Erih1; Erih2; Gm1548; Nap5
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225037 representing NM_001081756 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGGGAAGAGACAGCTGGATAGAAGGGACTTTGGTAAAAGACTCTCTCTGGACAGCAGTCTTGTGG AATACATGGACTCTAACAAATGCATCGAGCACCTGCTGGCACAACTCAGGGAGCAACACAGAAGTCTCTG GCGGGAGAAGCTGGCTGTGGCCAGGCTGCAGAGAGAAGTTGCCCAAAGGACAAGCCAAGGGGCCATGCAC GAGAAGCTGATACATGAGTTGGAAGAGGAACGGCACTTGCGGCTACAGAGTGAGGAGCGGCTGCGGGAGG TGACCCTGGAGTCAGAGCGCAACAGGATCCAGATGCTCGGCCTGCAGCAGCAGTTTTCCAGAATGGAAGA AACTGTCCGAAGTCTGTTGCAGAGCCAAGGATCTCCAGAGCAGAAAAAGGAAGAACCTGCCAAGATCACA GCCTACCAGGAAGGACTACCTGATGAAGAGAGAAAAGAGCAAGCAGCACTGGGTGATCTCCATGTGGTGG CAGCTGATGAGGACTCAAGAAGCGAGCACAGTAGTGTGGAGGAGGGAAAGGAAGACAGCAGACTGCTCCT GAAGAGACTGAAGGCCCTGGAGGCGGAGAATTCAGCACTGGCACTGGAAAACGAGAATCAGAGAGAACAG TATGAGCGATGTCTTGATGAGGTAGCCAACCAAGTTGTTCGAGCTCTACTCACTCAAAAGGATCTGAGGG AGGAGTGTGTAAAGTTGAAAACAAGAGTGTTTGACTTGGAGCAACAGAACCGAGCACTCAGCCTCCTGTT CCAACAGCGAGTCAGACCAGCTTCTGATGTGCTTCTCCAGGAACACCTCCAGAATGCAAAATCAGGAACC CCTGCCTTGAAACACTCTGGCTCAGGGGTTGTCATCCCTGATCACCTCTGTCCTCGCAACAGCTGCAGCA GTGGCAGTGAGCTATCGCTGTCAAGCACCTGCAGCGAGTACTCCAGTGGCTCCTCCTACACGTGGCAAGA CAGGAAGCTCATTGGGAAAAGGCAATCATCGCGGATCTGGGATAAAAGACTAAGTATTGATTCTTCGCTC CCAAGTGGCTTCGCCAGCCCTGCAGATGAACTACCTCCGACCCGTATCAAAGAAAGCCACATTCTAGAGG GACTGAGGAAGCTACAGAAGCGGAAAGTGTTCCTTGAATCACCATCATTGGTGATAAGCAAATGGGGTTA TAAAGATTGCATGAACTCGAATGAAGGGATCTATTCGCCAGGAATTAAAACCAGCAGCCTCAAGGAGTGT CCGCCCTGCCAACCGTCTGGCATGGGGATCCCCTGCCAGAATCTCCATCAGACATTCATCTATGATGCAA ATTCTCATGAGGATGCACATGAAGAACTCCCATCCTCAGCCTTGCCACTAACATCTCCGAGCCAGGGCTA CAGAATACAGGGCAGTAAATTGACACACAGTGTTTCAGACAGTCTGTTTAACTGGGAGCCACATAGGAAG TACTTTGCAGAAGGTACAGCCTCAGTATATACCAGAGAAATGCCTGAAAGGCTAGCTCGTTGTGCTAGCC ACTGCCCCTTGGAGAGGAAGCTATGTCCCAGTATACACCTGCCCTGGGTGCAAAGAGACAGGGACCCACA CAGCCAAGTTCATGGCCATGTGCCTCTGAACCTGCAGCTCTCAGATACCGATGACAATGAGACCCTAAAC GAGCTACACATAGAGAGCAGTGATGAGAGAAGCCCTTCAGACCTGTCCTTGACTGGGGACACCGACAAGT CCATGGAGAACCTAGAGGTGGGATTCAGAAAGTCTGAAGGTGGCTCTCGGGACATGGGAGAAGAAGCATG TCCCACCCACTTAGAGACTAGCCCGAAGGCATTCAGTTTCATCAAGCAACAAAGGGTTGTCAAGAGGACC TCTTCGGAAGAATGTGTCACGGTCATATTTGATGCAGAAAATGGAGAGCCAGTGGAATTCAGCTCTCACC AGGCTGGAGTCGTCACTGTCACCAGGGATGAAATTTCCATCAGCTCAGCCCTTGCTGGGCCCTCAGAGGA ACTCACTGAACTTTCACCTCAGGAAATTACTCACTTCCAGGCAGGAGCTACTGCAAGAGACTGTCCTTTT CTGAAGAGACCTGAAGAGGAGACTGCGAAAAACATTCCAAAAGATGAAAATCATATTCTCACAGCTCCGA ATGACTTCTTCAGCTCCCAGACTATAATAACTCAGAATTCCCCAAGACCAAAGCTCGCCAAACCCACACA GGGGGTAGCCTGGCAGAGTAATTCTAGACCTGTTGGAAACATGGGTGTCTACACAAAGCAAGGTCTGACA AAAATCCCTTCCAGAGGCAAGTCTTCACCTCAGAAATCCAAACTTGTGGAGTCTATGCCCACCATGCTGG TCCCCTCCAGTGCCCTAGTGACTCCTGAAAAGACACCAGCATCCCCTATGGGGAAACTCTCACAATGTAG AAAGGCAGAGAGCCCTGGTTCTCTCTGTGATGTACAGCCAGAATCACACATTCCAAAACACCCTGCCCAG GTTCCTCATATCCCCAAAATTTCCAATAAGAAGAACTGGGTTCAGTGCCCCAGGAGTCAGACTTCAGGGA TCCAGTCACGGCATCTCATGGGGAGAAGTGACAGTAGTGAAATGAGGTCCAATGGCTGTGTCTCTGCGCC ACAGACAAGAATGAGGTCTCCCTCCCCTCCAACCCCTCCAGGGAGATCAGTGTCACTCCTGGTTAGACCC AGTTATGATTACCCACCACTACCATCACCTGCCAAACCTGAAACTCAGGGCCCTGGAGACGCTGTAAGTC CAGGGTTGAAATCCCCGCTGTTGAAAGGATCCTCTGCTCCTCTTGTCTCTTCCAGTCCAGTGATGTCAGA AATACAGAAGAAACCATCTGTGGCCTCCAGAAAGTCTGCCTTCCCTCCAGCTCTTCCCTCAACCCAAGCG GTAGTGCACACCAGATACCCTGAACACATAGCCTCCAGTCCATTTGCTGTGACGACTTCAGGGCCCCCAA AAGTCTCTCCAAAAAGAGGCATCCCTAAACCTCCCCCTCACCATGCACTAGGAACTGCCCACATGGACAC AGACTTACAGACACCCAAGACTTGTCCTTCAAGCTGTGAACTGCTAGAGGTGACATCTTGTAAAAGCCTG TCTCCAGGGAGAAAAAGACAGTTGAGTGACAGCACTGTGATGCCCCACAGGCCTTCCTTCTCAGGAGTGA ATGAGTCCTTGACACCTCAGGTTAACAGTCCATCCTCCTCTTCCTCATCCTGTAGAAACCAAGGGTCCTC ACAAGGTGGCCAGAACTCTCCCGAGAAAGGACCTAAAACTCGCCTCCCAGTAGGACTCAAAGTTCTCATG AAGTCTCCTCAGTTGCTCAAGAAAACTTCCTTGGTACCAGGGAAACAAGAAAAGGATAGTTTAAATGAAG CATCTCGAAGCTGTGTAGCAGCAAACACGTCTAATCCTGAGGACTCCAAAGACCCTGCAAGTCTGGAGAC CTCAAGAGATGAGAGAACCACAGCCTTACTAGGTGTGAGAGGTCCCGACAGTCTAGCTAGAGGACTTGCC TTAGACTCAACAATGCCAGAATCGTTAGAATGTAGCATCTCCAGAGATGATGGAAGAGATGGGGCAGACA GCAGAGCCATAAAAAGATCCTTCTCCTCCAGCAAGCCACACCTAAAGCCTGCTCTAGGCATGAATGGAGC CAAAGCTCGGAGTCAGAGCTTCAGCATCCACTTGGGAGAGAAGCCTTCCACACCCCCAACAGAAGGGCCA GGCAAGATTCGAACTCAGATTATCACCAACACGGCTGAAAGAGGCAACTCCCTCACCAGACAGAATATCT CAATGGAAGGGTCCCCAAGCAAGACCCCCTCTGTCTCAACATCTGATAATCTGCCCAATGCTGGAAGGCC CCTGGGAAACCCCTTTTCCAGGCAGAGTTCTCTCTGTAGCACCGGAAGTTCTAGCAGCCAGCACGGAAGC CCAAGCAAGTTACCCTTGAGGATGCTCACAAGGCCGGAAGGACTCCTCACTCCTGGAGTGGAAGACCAAC GGCTCTCTACTCAGGAAGAGTGTCCTGAAAGAGGTGTACATGAGCAATCTAGCAGTGACCACCATAGGCA TCCATCTCCTCTTTCAGACTGCCCTCAGTTCCTGCACGTTCCAGGGAGGATGCAGCGCCCAAGCAATTTG GAAACAAGTGGGATATCTAAGCTAGAAACTTCTGGAAGGCATCCAGATGCTTCTTCAACTGGGACTAGTG TCGTGAGCCCAGAAGCCCCCCTCTCACCCTCCATCGAAGAAAAGGTCATGTTGTGCATTCAGGAGAATGT GGAAAAGGGCCAAGTGCAGACAAAGTCTGCCTCTGTGGAAGTGAAGCCCAGGGCAGGGCCTTCGTTCACC AGCTGGTTTGGTTTTCGAAAGAGCAGGCTTCCAGCTCTCAGTAGCAGGAGAATGGAAGCTTCCAAGAGTA AAGTGGAAAAGAAAGACACAAAACTGAAATCTGAGAGGAAAAAAGAAAAGAAGAAAGCTGAAGTACAATC AAAGATAGAAAACGAACTGAGTAGGGGGACCAAGAAGGCAGATGGTCAGAGTCCAGACGATGGTCTGCAA AGCTCAGAGAGTCTGAAAGTATCCCAAGACATTTATAACCAAATGAAGTTGGAGCCAAGGAATAGACCCA GCCCTGCTGTGTGCCCAACAAAAGATGCCTTCATGACAGAACTTCTAAACAGAGTTGATAAGAGAGCCTC GGTACAGAGAGAGAGTGGATCAAAGGGTGTTTCCTGCAGGAGCGTGTTAGAGGGCACCTCCCAGGGCTCC TGTTTCGCCAGCGGCTCTGTGAGCACTCAAGGAAGTCAAAAGAAAAACATCAAAACCAAAGTCGATATGG AAAAACCAAGAGAATCCTTGGGGGCCGAGGTCAATGAAGATGTACAAGAAGATGAAGAAGATAGAGTTGC TGACACTACTCTCCAGAGCCACATGATAGAATCCAACTGCCAGATGAGAACACTGGACAGCGGGATTGGA ACCTTTCCCCTCCCAGACTCAGGAAACCGCTCAGTGGGACGATACATATGCCAGCAGGACTCTCCAGAGG ACACTGATGCCCTCCTGCCTCTCCAGCCAGCCGCTTCCATGGCATCCTCTGCCAGAGCCCAAACCCTTGA CCGCGAAGTGCCTTCCTCCACAGACAGCCAGAGCTCCCCGGAGTCCATGATCATCCATTCCATATCTGAC CCCAGCATGATGGCCAGAGAGATGAGAGCCTCACAGAGTCACCTTCCCAAGCCTACCTCCTCAGGAAAAC CTAGTTTTGAAAAGCAAAATGGAGCAGAGTCAAAGGCTCACACTGGCCCATCATTCGAATATGCTGAGGA TGCAGTGGCAAGTGAGCCCCTTTCAGGATGGAGGGGTGAAGATGCTACTACTGAGACCCAGGAACCGAAA CAAGTTGAAGAAACAAAAGAAGATCCTGAGAATAGATTATCTGAAATTTCCCTGGAGTCATTCAATAAAT TTAGCAGCAATACTGTGATTTTATTAGGAAAAGAGAAGTCTCCAAGTGAGGTTGAAGGACAAAAGGAAGA GAAAGGCAAAAAGACAGAAGTGTCTTCGAGTAGCTCCGAACGGCCTGGGGTGGACCGCGTGGAGTCTCTG AGTGACTCTCTGTATGACAGTTTCTCCTCTTGTGCAAGTCAAGCATCCAACGAGGCCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001081756
Insert Size	5730 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001081756.1, NP_001075225.1
RefSeq Size	7246 bp
RefSeq ORF	5730 bp
Locus ID	210356

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